Yong Jun Goh
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View article: Lactobacillus acidophilus Expressing Murine Rotavirus VP8 and Mucosal Adjuvants Induce Virus-Specific Immune Responses
Lactobacillus acidophilus Expressing Murine Rotavirus VP8 and Mucosal Adjuvants Induce Virus-Specific Immune Responses Open
Rotavirus diarrhea-associated illness remains a major cause of global death in children under five, attributable in part to discrepancies in vaccine performance between high- and low-middle-income countries. Next-generation probiotic vacci…
View article: Genomic and epigenetic landscapes drive CRISPR-based genome editing in<i>Bifidobacterium</i>
Genomic and epigenetic landscapes drive CRISPR-based genome editing in<i>Bifidobacterium</i> Open
Bifidobacterium is a commensal bacterial genus ubiquitous in the human gastrointestinal tract, which is associated with a range of health benefits. The advent of CRISPR-based genome editing technologies provides opportunities to investigat…
View article: <i>In Vivo</i> Transcriptome of Lactobacillus acidophilus and Colonization Impact on Murine Host Intestinal Gene Expression
<i>In Vivo</i> Transcriptome of Lactobacillus acidophilus and Colonization Impact on Murine Host Intestinal Gene Expression Open
To date, our basis for comprehending the probiotic mechanisms of Lactobacillus acidophilus , one of the most widely consumed probiotic microbes, was largely limited to in vitro functional genomic studies. Using a germfree murine colonizati…
View article: Portable CRISPR-Cas9 <sup>N</sup> System for Flexible Genome Engineering in Lactobacillus acidophilus, Lactobacillus gasseri, and Lactobacillus paracasei
Portable CRISPR-Cas9 <sup>N</sup> System for Flexible Genome Engineering in Lactobacillus acidophilus, Lactobacillus gasseri, and Lactobacillus paracasei Open
This work describes the development of a lactobacillus CRISPR-based editing system for genome manipulations in three Lactobacillus species belonging to the lactic acid bacteria (LAB), which are commonly known for their long history of use …
View article: S-layer associated proteins contribute to the adhesive and immunomodulatory properties of Lactobacillus acidophilus NCFM
S-layer associated proteins contribute to the adhesive and immunomodulatory properties of Lactobacillus acidophilus NCFM Open
View article: An 1,4-α-Glucosyltransferase Defines a New Maltodextrin Catabolism Scheme in Lactobacillus acidophilus
An 1,4-α-Glucosyltransferase Defines a New Maltodextrin Catabolism Scheme in Lactobacillus acidophilus Open
The degradation of starch in the small intestine generates short linear and branched α-glucans. The latter are poorly digestible by humans, rendering them available to the gut microbiota, e.g., lactobacilli adapted to the small intestine a…
View article: The S-layer Associated Serine Protease Homolog PrtX Impacts Cell Surface-Mediated Microbe-Host Interactions of Lactobacillus acidophilus NCFM
The S-layer Associated Serine Protease Homolog PrtX Impacts Cell Surface-Mediated Microbe-Host Interactions of Lactobacillus acidophilus NCFM Open
Health-promoting aspects attributed to probiotic microorganisms, including adhesion to intestinal epithelia and modulation of the host mucosal immune system, are mediated by proteins found on the bacterial cell surface. Notably, certain pr…
View article: Deletion of S-Layer Associated Ig-Like Domain Protein Disrupts the Lactobacillus acidophilus Cell Surface
Deletion of S-Layer Associated Ig-Like Domain Protein Disrupts the Lactobacillus acidophilus Cell Surface Open
Bacterial surface-layers (S-layers) are crystalline arrays of repeating proteinaceous subunits that coat the exterior of many cell envelopes. S-layers have demonstrated diverse functions in growth and survival, maintenance of cell integrit…
View article: Comparative Analysis of Lactobacillus gasseri and Lactobacillus crispatus Isolated From Human Urogenital and Gastrointestinal Tracts
Comparative Analysis of Lactobacillus gasseri and Lactobacillus crispatus Isolated From Human Urogenital and Gastrointestinal Tracts Open
Lactobacillus crispatus and Lactobacillus gasseri are two of the main Lactobacillus species found in the healthy vaginal microbiome and have also previously been identified and isolated from the human gastrointestinal …
View article: A study on decompresses heat pump dryer for drying of shiitake mushrooms at medium temperature
A study on decompresses heat pump dryer for drying of shiitake mushrooms at medium temperature Open
This study developed a drying method and a dryer that are optimized for shiitake mushrooms. They are mainly being cultivated and consumed as food and medicine in Korea, Japan, China, and Southeast Asia. They generally contain lots of moist…
View article: Genome editing using the endogenous type I CRISPR-Cas system in <i>Lactobacillus crispatus</i>
Genome editing using the endogenous type I CRISPR-Cas system in <i>Lactobacillus crispatus</i> Open
CRISPR-Cas systems are now widely used for genome editing and transcriptional regulation in diverse organisms. The compact and portable nature of class 2 single effector nucleases, such as Cas9 or Cas12, has facilitated directed genome mod…
View article: Outcomes and characterization of chromosomal self-targeting by native CRISPR-Cas systems in <i>Streptococcus thermophilus</i>
Outcomes and characterization of chromosomal self-targeting by native CRISPR-Cas systems in <i>Streptococcus thermophilus</i> Open
CRISPR-Cas systems provide adaptive immunity against phages in prokaryotes via DNA-encoded, RNA-mediated, nuclease-dependent targeting and cleavage. Due to inefficient and relatively limited DNA repair pathways in bacteria, CRISPR-Cas syst…
View article: <i>Lactobacillus acidophilus</i> Metabolizes Dietary Plant Glucosides and Externalizes Their Bioactive Phytochemicals
<i>Lactobacillus acidophilus</i> Metabolizes Dietary Plant Glucosides and Externalizes Their Bioactive Phytochemicals Open
Therapeutically active glycosylated phytochemicals are ubiquitous in the human diet. The human gut microbiota (HGM) modulates the bioactivities of these compounds, which consequently affect host physiology and microbiota composition. Despi…
View article: Investigating the Effect of Growth Phase on the Surface-Layer Associated Proteome of Lactobacillus acidophilus Using Quantitative Proteomics
Investigating the Effect of Growth Phase on the Surface-Layer Associated Proteome of Lactobacillus acidophilus Using Quantitative Proteomics Open
Bacterial surface-layers (S-layers) are semi-porous crystalline arrays that self-assemble to form the outermost layer of some cell envelopes. S-layers have been shown to act as scaffolding structures for the display of auxiliary proteins e…
View article: The S-layer Associated Serine Protease Homolog PrtX Impacts Cell Surface-Mediated Microbe-Host Interactions of Lactobacillus acidophilus NCFM
The S-layer Associated Serine Protease Homolog PrtX Impacts Cell Surface-Mediated Microbe-Host Interactions of Lactobacillus acidophilus NCFM Open
Health-promoting aspects attributed to probiotic microorganisms, including adhesion to intestinal epithelia and modulation of the host mucosal immune system, are mediated by proteins found on the bacterial cell surface. Notably, certain pr…
View article: An Extracellular Cell-Attached Pullulanase Confers Branched α-Glucan Utilization in Human Gut Lactobacillus acidophilus
An Extracellular Cell-Attached Pullulanase Confers Branched α-Glucan Utilization in Human Gut Lactobacillus acidophilus Open
Of the few predicted extracellular glycan-active enzymes, glycoside hydrolase family 13 subfamily 14 (GH13_14) pullulanases are the most common in human gut lactobacilli. These enzymes share a unique modular organization, not observed in o…
View article: Deletion of Lipoteichoic Acid Synthase Impacts Expression of Genes Encoding Cell Surface Proteins in Lactobacillus acidophilus
Deletion of Lipoteichoic Acid Synthase Impacts Expression of Genes Encoding Cell Surface Proteins in Lactobacillus acidophilus Open
Lactobacillus acidophilus NCFM is a well-characterized probiotic microorganism, supported by a decade of genomic and functional phenotypic investigations. L. acidophilus deficient in lipoteichoic acid (LTA), a major immunosti…
View article: Outside front cover: Differential proteome and cellular adhesion analyses of the probiotic bacterium <i>Lactobacillus acidophilus</i> NCFM grown on raffinose – an emerging prebiotic
Outside front cover: Differential proteome and cellular adhesion analyses of the probiotic bacterium <i>Lactobacillus acidophilus</i> NCFM grown on raffinose – an emerging prebiotic Open
DOI: 10.1002/pmic.201500212 Lactobacillus acidophilus NCFM is a commonly used and well-studied probiotic bacterium. By the present differential proteome analysis, new insights into its synbiotic combination with the emerging prebiotic raff…
View article: Contents: Proteomics 9'16
Contents: Proteomics 9'16 Open
Lactobacillus acidophilus NCFM is a commonly used and well-studied probiotic bacterium.By the present differential proteome analysis, new insights into its synbiotic combination with the emerging prebiotic raffinose through comparative who…
View article: Prebiotics: why definitions matter
Prebiotics: why definitions matter Open
View article: <scp>SIGNR</scp> 3‐dependent immune regulation by <i>Lactobacillus acidophilus</i> surface layer protein A in colitis
<span>SIGNR</span> 3‐dependent immune regulation by <i>Lactobacillus acidophilus</i> surface layer protein A in colitis Open