Yongjian Xiao
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View article: Theoretical Framework for Absolute Quantification in Digital Immunoassays and Advancements in Microfluidic-Free Droplet-Based Digital Immunoassay Methodologies
Theoretical Framework for Absolute Quantification in Digital Immunoassays and Advancements in Microfluidic-Free Droplet-Based Digital Immunoassay Methodologies Open
Conventional single-molecule immunoassay models, which model on a single discrete Poisson distribution, are inadequate for achieving theoretical absolute quantification of target proteins. We present a new method that models the capture of…
View article: A Theoretical Framework for Absolute Quantification in Digital Immunoassays and the Advancements in Droplet-Based Digital Immunoassay Methodologies
A Theoretical Framework for Absolute Quantification in Digital Immunoassays and the Advancements in Droplet-Based Digital Immunoassay Methodologies Open
Conventional single-molecule immunoassay models, which model on a single discrete Poisson distribution, are inadequate for achieving theoretical absolute quantification of target proteins. We introduce a novel approach that models the capt…
View article: Development of Absolute Quantification Methods for Digital Immunoassays: Theoretical Framework and Establishment of Droplet-Based Digital Immunoassay Techniques
Development of Absolute Quantification Methods for Digital Immunoassays: Theoretical Framework and Establishment of Droplet-Based Digital Immunoassay Techniques Open
This paper analyzes the current digital Immunoassays techniques and their theoretical foundations. Traditional single-molecule immunoassay models based on a single discrete Poisson distribution cannot explain or achieve absolute quantifica…
View article: Cold-Sintered ZnO Ceramic Composites Co-Doped with Polytetrafluoroethylene and Oxides
Cold-Sintered ZnO Ceramic Composites Co-Doped with Polytetrafluoroethylene and Oxides Open
Grain boundaries play a significant role in determining the performance of ceramic-based materials. The modulation of interfacial structures provides a promising approach to improve the physicochemical and electrical properties of ceramic …
View article: Potential diagnostic value of miRNAs in sexually transmitted infections
Potential diagnostic value of miRNAs in sexually transmitted infections Open
MiRNAs are small endogenous non-coding RNAs that have been demonstrated to be involved in post-transcriptional gene silencing, regulating a number of metabolic functions in the human body, including immune response, cellular physiology, or…
View article: A Promising Tool in Serological Diagnosis: Current Research Progress of Antigenic Epitopes in Infectious Diseases
A Promising Tool in Serological Diagnosis: Current Research Progress of Antigenic Epitopes in Infectious Diseases Open
Infectious diseases, caused by various pathogens in the clinic, threaten the safety of human life, are harmful to physical and mental health, and also increase economic burdens on society. Infections are a complex mechanism of interaction …
View article: Laboratory Diagnostic Tools for Syphilis: Current Status and Future Prospects
Laboratory Diagnostic Tools for Syphilis: Current Status and Future Prospects Open
With the increasing number of patients infected with syphilis in the past 20 years, early diagnosis and early treatment are essential to decline syphilis prevalence. Owing to its diverse manifestations, which may occur in other infections,…
View article: Identification of key genes and pathways in syphilis combined with diabetes: a bioinformatics study
Identification of key genes and pathways in syphilis combined with diabetes: a bioinformatics study Open
View article: Additional file 1 of Identification of key genes and pathways in syphilis combined with diabetes: a bioinformatics study
Additional file 1 of Identification of key genes and pathways in syphilis combined with diabetes: a bioinformatics study Open
Additional file 1. Sample information and sequencing data of syphilis combined with diabetes and controls.
View article: The N‐terminal D1 domain of <i>Treponema pallidum </i>flagellin binding to TLR5 is required but not sufficient in activation of TLR5
The N‐terminal D1 domain of <i>Treponema pallidum </i>flagellin binding to TLR5 is required but not sufficient in activation of TLR5 Open
Syphilis is a chronic bacterial infection caused by Treponema pallidum ( T pallidum ) and the pathogenesis that T pallidum infection induces immunopathological damages in skin and other tissues remains unclear. We have previously reported …
View article: Immunogenicity and protective efficacy against <i>Treponema pallidum</i> in New Zealand rabbits immunized with plasmid DNA encoding flagellin
Immunogenicity and protective efficacy against <i>Treponema pallidum</i> in New Zealand rabbits immunized with plasmid DNA encoding flagellin Open
Plasmid DNA encoding flagellin FlaB3 was used as a vaccination candidate for the evaluation of immunogenicity and protection against Treponema pallidum subsp. pallidum dissemination. First, intramuscular injection of the flag…
View article: Immunogenicity and protective efficacy against <i>Treponema pallidum</i> in New Zealand rabbits immunized with plasmid DNA encoding flagellin
Immunogenicity and protective efficacy against <i>Treponema pallidum</i> in New Zealand rabbits immunized with plasmid DNA encoding flagellin Open
Plasmid DNA encoding flagellin FlaB3 was used as a vaccination candidate for the evaluation of immunogenicity and protection against Treponema pallidum subsp. pallidum dissemination. First, intramuscular injection of the flagellin encoded …
View article: The outer membrane protein Tp92 of <i>Treponema pallidum</i> induces human mononuclear cell death and <scp>IL</scp>‐8 secretion
The outer membrane protein Tp92 of <i>Treponema pallidum</i> induces human mononuclear cell death and <span>IL</span>‐8 secretion Open
Treponema pallidum is the pathogen that causes syphilis, a sexually transmitted disease; however, the pathogenic mechanism of this organism remains unclear. Tp92 is the only T. pallidum outer membrane protein that has structural features s…
View article: Intramuscular primary immunization by nucleic acid vaccine pcDNA/Gpd‑IL‑2 and enhanced immunization with mucosal adjuvant CpG‑ODN and Gpd‑IL‑2 recombinant protein effectively induced strong mucosal immune responses and immune protective effects against Treponema pallidum skin infection
Intramuscular primary immunization by nucleic acid vaccine pcDNA/Gpd‑IL‑2 and enhanced immunization with mucosal adjuvant CpG‑ODN and Gpd‑IL‑2 recombinant protein effectively induced strong mucosal immune responses and immune protective effects against Treponema pallidum skin infection Open
The present study aimed to evaluate the immune effect of intramuscular primary immunization by the nucleic acid vaccine pcDNA/glycerophosphodiester phosphodiesterase-interleukin-2 (pcDNA/Gpd-IL-2) and enhanced immunization 2 weeks later wi…
View article: Screening and identification of immunoactive FlaB protein fragments of Treponema pallidum for the serodiagnosis of syphilis
Screening and identification of immunoactive FlaB protein fragments of Treponema pallidum for the serodiagnosis of syphilis Open
Flagellin is a classical pathogen-associated molecular pattern that can evoke a robust immune response. We have demonstrated previously that three full-length flagellins of Treponema pallidum, namely FlaB1, FlaB2 and FlaB3, did have diagno…
View article: Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay for the Detection of Treponema pallidum DNA in the Peripheral Blood of Secondary Syphilis Patients
Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay for the Detection of Treponema pallidum DNA in the Peripheral Blood of Secondary Syphilis Patients Open
Secondary syphilis (SS) has always been puzzling for the clinicians because of the similarity of the appearance of skin rashes with other dermatoses. Serological assays are useful, but less sensitive at an early stage of SS or when patient…
View article: A novel ELISA using a recombinant outer membrane protein, rTp0663, as the antigen for serological diagnosis of syphilis
A novel ELISA using a recombinant outer membrane protein, rTp0663, as the antigen for serological diagnosis of syphilis Open
View article: Factors associated with syphilis infection: a comprehensive analysis based on a case-control study
Factors associated with syphilis infection: a comprehensive analysis based on a case-control study Open
SUMMARY This study aimed to comprehensively evaluate factors that influence the likelihood of syphilis infection from risk-taking behaviours and medical conditions. A retrospective case-control study was conducted by enrolling 664 syphilis…