Yongxi Zhao
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View article: Mechanical Constraints Promote Noncanonical Base‐Pairing Interactions
Mechanical Constraints Promote Noncanonical Base‐Pairing Interactions Open
Mechanical perturbations profoundly influence nucleobase interactions in cells, serving as key modulators of fundamental cellular processes such as transcriptional regulation. However, their precise roles remain elusive due to methodologic…
View article: Construction And Molecular Characterization Of Rare Salivary Gland Tumor Organoids
Construction And Molecular Characterization Of Rare Salivary Gland Tumor Organoids Open
Aim or purpose: To establish a personalized research model for rare subtypes of salivary gland tumor, bridging foundational investigations and clinical applications to advance precision oncology in this understudied field. Materials and me…
View article: Microneedle-Based DNA Tension Gauge Tethers Enable In Vivo Monitoring of Cell Mechanics during Skin Tissue Regeneration
Microneedle-Based DNA Tension Gauge Tethers Enable In Vivo Monitoring of Cell Mechanics during Skin Tissue Regeneration Open
The mechanical force exerted by dermal fibroblasts is crucial for promoting cutaneous tissue regeneration and wound healing. However, the implantation of a force interface in vivo or within tissue has become a new challenge in measuring me…
View article: ABC‐seq Expands Small RNAs Profiling with Successive Nucleic Acid Structure‐Differentiated Enzymatic Recognition
ABC‐seq Expands Small RNAs Profiling with Successive Nucleic Acid Structure‐Differentiated Enzymatic Recognition Open
Small RNAs (smRNAs) play crucial roles in gene regulation, but current sequencing methods face a set of tangled challenges, including adapter ligation bias, high adapter dimer byproducts, and amplification variability, which limit their ca…
View article: Location‐Differentiated DNA Hierarchical Cyclic Construction Demarcating Identical Biomolecules Across Cell Membrane Microenvironments
Location‐Differentiated DNA Hierarchical Cyclic Construction Demarcating Identical Biomolecules Across Cell Membrane Microenvironments Open
Local molecular composition across cell membrane microenvironments exerts profound effects on cellular physiology and pathology. Yet spatial demarcation of identical biomolecules inside and outside lipid raft microdomains has not been dete…
View article: Composite Mapping for Peptide‐Based Data Storage with Higher Coding Density and Fewer Synthesis Cycles
Composite Mapping for Peptide‐Based Data Storage with Higher Coding Density and Fewer Synthesis Cycles Open
Peptides are natural information‐bearing mediums and are promising for high‐density data storage. However, conventional mapping of one amino acid (AA) to one binary code has limited the improvement of coding density by increasing the total…
View article: Proximity-activated DNA scanning encoded sequencing for massive access to membrane proteins nanoscale organization
Proximity-activated DNA scanning encoded sequencing for massive access to membrane proteins nanoscale organization Open
Cellular structure maintenance and function regulation critically depend on the composition and spatial distribution of numerous membrane proteins. However, current methods face limitations in spatial coverage and data scalability, hinderi…
View article: Sequential DNA Coding for Programmable Information Encryption
Sequential DNA Coding for Programmable Information Encryption Open
DNA molecules, with highly variable sequences and inherent programmability, emerge as a promising material for next‐generation information storage and data encryption. However, due to the singular encryption method or limited randomness of…
View article: DNA‐Encoded Fluorescence Signals for Imaging Analysis
DNA‐Encoded Fluorescence Signals for Imaging Analysis Open
Fluorescence imaging has been a powerful technique for the visualization of interest biomolecules. It is widely used in basic biological research, clinical medicine, and other fields. However, the fluorescence signals are often too weak fo…
View article: Molecule Differentiation Encoding Microscopy to Dissect Dense Biomolecules in Cellular Nanoenvironments below Spatial Resolution
Molecule Differentiation Encoding Microscopy to Dissect Dense Biomolecules in Cellular Nanoenvironments below Spatial Resolution Open
Cellular biomolecules may exhibit dense distribution and organization at the nanoscale to govern vital biological processes. However, it remains a common challenge to digitize the spatially dense biomolecules under the spatial resolution o…
View article: Molecule Differentiation Encoding Microscopy to Dissect Dense Biomolecules in Cellular Nanoenvironments below Spatial Resolution
Molecule Differentiation Encoding Microscopy to Dissect Dense Biomolecules in Cellular Nanoenvironments below Spatial Resolution Open
Cellular biomolecules may exhibit dense distribution and organization at the nanoscale to govern vital biological processes. However, it remains a common challenge to digitize the spatially dense biomolecules under the spatial resolution o…
View article: ABC-seq expands small RNAs content with randomized adapter pools operation
ABC-seq expands small RNAs content with randomized adapter pools operation Open
Cellular gene expression is widely regulated by a large number of small RNAs (smRNAs). The ability to in-depth analysis of smRNAs content is vital for comprehensive understanding their architecture and function. However, experimental appro…
View article: Ionic Strength‐Mediated “DNA Corona Defects” for Efficient Arrangement of Single‐Walled Carbon Nanotubes
Ionic Strength‐Mediated “DNA Corona Defects” for Efficient Arrangement of Single‐Walled Carbon Nanotubes Open
Single‐stranded DNA oligonucleotides wrapping on the surface of single‐walled carbon nanotubes (SWCNTs), described as DNA corona, are often used as a dispersing agent for SWCNTs. The uneven distribution of DNA corona along SWCNTs is relate…
View article: Microwell array chip-based single-cell analysis
Microwell array chip-based single-cell analysis Open
This review summarizes microwell array chip-based single-cell analysis of versatile biomolecules including nucleic acids, proteins and metabolites.
View article: Hierarchical DNA branch assembly-encoded fluorescent nanoladders for single-cell transcripts imaging
Hierarchical DNA branch assembly-encoded fluorescent nanoladders for single-cell transcripts imaging Open
Spatial visualization of single-cell transcripts is limited by signal specificity and multiplexing. Here, we report hierarchical DNA branch assembly-encoded fluorescent nanoladders, which achieve denoised and highly multiplexed signal ampl…
View article: CRISPR/Cas system-guided plasmid mutagenesis without sequence restriction
CRISPR/Cas system-guided plasmid mutagenesis without sequence restriction Open
Plasmid mutagenesis is an essential step to engineer protein variants with desired properties. Direct generation of mutations on plasmids is a promising method compared to PCR-based methods and subcloning, yet it suffers from the sequence …
View article: Cellular macromolecules-tethered DNA walking indexing to explore nanoenvironments of chromatin modifications
Cellular macromolecules-tethered DNA walking indexing to explore nanoenvironments of chromatin modifications Open
Exploring spatial organization and relationship of diverse biomolecules within cellular nanoenvironments is important to elucidate the fundamental processes of life. However, it remains methodologically challenging. Herein, we report a mol…
View article: Research on Design Method of Half-Wavelength Tuning Net
Research on Design Method of Half-Wavelength Tuning Net Open
View article: Nucleic Acids Analysis
Nucleic Acids Analysis Open
View article: Click-encoded rolling FISH for visualizing single-cell RNA polyadenylation and structures
Click-encoded rolling FISH for visualizing single-cell RNA polyadenylation and structures Open
Spatially resolved visualization of RNA processing and structures is important for better studying single-cell RNA function and landscape. However, currently available RNA imaging methods are limited to sequence analysis, and not capable o…
View article: Locus-patterned sequence oriented enrichment for multi-dimensional gene analysis
Locus-patterned sequence oriented enrichment for multi-dimensional gene analysis Open
A locus-patterned sequence oriented enrichment was proposed to analyze the gene sequence, locus variation and molecular abundance of heterogeneous genes.
View article: DHX9 inhibits epithelial-mesenchymal transition in human lung adenocarcinoma cells by regulating STAT3.
DHX9 inhibits epithelial-mesenchymal transition in human lung adenocarcinoma cells by regulating STAT3. Open
DHX9 has numerous functions regulating transcription, translation, RNA processing and transport, and DNA replication and maintenance of genomic stability. It is involved in human cancers as either an oncogene or tumor suppressor. However, …
View article: Programming <i>in situ</i> accelerated DNA walkers in diffusion-limited microenvironments
Programming <i>in situ</i> accelerated DNA walkers in diffusion-limited microenvironments Open
In situ accelerated DNA walkers in diffusion-limited microenvironments are reported and utilized to monitor cytoplasmic enzyme catalysis and pathways.
View article: Sol–Gel Synthesis of Metal–Phenolic Coordination Spheres and Their Derived Carbon Composites
Sol–Gel Synthesis of Metal–Phenolic Coordination Spheres and Their Derived Carbon Composites Open
A formaldehyde‐assisted metal–ligand crosslinking strategy is used for the synthesis of metal–phenolic coordination spheres based on sol–gel chemistry. A range of mono‐metal (Co, Fe, Al, Ni, Cu, Zn, Ce), bi‐metal (Fe‐Co, Co‐Zn) and multi‐m…
View article: Engineered Janus probes modulate nucleic acid amplification to expand the dynamic range for direct detection of viral genomes in one microliter crude serum samples
Engineered Janus probes modulate nucleic acid amplification to expand the dynamic range for direct detection of viral genomes in one microliter crude serum samples Open
Janus probes were designed to expand the dynamic range of amplification for viral genome quantification in 1 μL crude serum.
View article: Single copy-sensitive electrochemical assay for circulating methylated DNA in clinical samples with ultrahigh specificity based on a sequential discrimination–amplification strategy
Single copy-sensitive electrochemical assay for circulating methylated DNA in clinical samples with ultrahigh specificity based on a sequential discrimination–amplification strategy Open
A sequential discrimination–amplification (SEDA) electrochemical strategy was constructed for the identification of single-copy circulating methylated DNA.
View article: Graphene oxide/core–shell structured metal–organic framework nano-sandwiches and their derived cobalt/N-doped carbon nanosheets for oxygen reduction reactions
Graphene oxide/core–shell structured metal–organic framework nano-sandwiches and their derived cobalt/N-doped carbon nanosheets for oxygen reduction reactions Open
A metal–organic framework (MOF) seed-mediated deposition route is developed to synthesize graphene oxide/core–shell structured MOF nano-sandwiches.
View article: Reliability and Economic Comparison of Ultra-long-distance Transmission Mode
Reliability and Economic Comparison of Ultra-long-distance Transmission Mode Open
Combining with the existing transmission technology which is feasible in engineering and theory, four types of ultra-long-distance transmission mode, in terms of UHV AC, UHV DC (±800 kV and ±1 000 kV) and Half-wavelength AC transmission sy…