Yoshihiro Hase
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View article: Characterization of Fucoxanthin-Deficient Strains in the Haptophyte <i>Tisochrysis lutea</i> Induced by Heavy-Ion Beam Irradiation
Characterization of Fucoxanthin-Deficient Strains in the Haptophyte <i>Tisochrysis lutea</i> Induced by Heavy-Ion Beam Irradiation Open
Although microalgae such as diatoms and haptophytes have been studied to optimize fucoxanthin production, the complete biosynthetic pathway of fucoxanthin remains unclear. In this study, we subjected the haptophyte Tisochrysis lutea cells …
View article: Agents to Achieve Goals in a Real-Time Environment in Video Games
Agents to Achieve Goals in a Real-Time Environment in Video Games Open
View article: Isolation and Characterization of High-Temperature-Tolerant Mutants of Bradyrhizobium diazoefficiens USDA110 by Carbon-Ion Beam Irradiation
Isolation and Characterization of High-Temperature-Tolerant Mutants of Bradyrhizobium diazoefficiens USDA110 by Carbon-Ion Beam Irradiation Open
Biofertilizers are promising technologies for achieving sustainable agriculture. However, high-temperature tolerance is a constraint that limits the function of microbial inoculants. To characterize the genetic changes responsible for the …
View article: Comparative analysis of seed and seedling irradiation with gamma rays and carbon ions for mutation induction in Arabidopsis
Comparative analysis of seed and seedling irradiation with gamma rays and carbon ions for mutation induction in Arabidopsis Open
The molecular nature of mutations induced by ionizing radiation and chemical mutagens in plants is becoming clearer owing to the availability of high-throughput DNA sequencing technology. However, few studies have compared the induced muta…
View article: Proton-Cluster-Beam Lethality and Mutagenicity in Bacillus subtilis Spores
Proton-Cluster-Beam Lethality and Mutagenicity in Bacillus subtilis Spores Open
The unique energy transfer characteristics of swift cluster ions have attracted the attention of many researchers working on the analysis or processing of material surfaces, but the effects on living organisms remain unclear. We irradiated…
View article: Characterization of gamma irradiation-induced mutations in Arabidopsis mutants deficient in non-homologous end joining
Characterization of gamma irradiation-induced mutations in Arabidopsis mutants deficient in non-homologous end joining Open
To investigate the involvement of the non-homologous end joining (NHEJ) pathway in plant mutagenesis by ionizing radiation, we conducted a genome-wide characterization of the mutations induced by gamma rays in NHEJ-deficient Arabidopsis mu…
View article: A Soybean Deletion Mutant That Moderates the Repression of Flowering by Cool Temperatures
A Soybean Deletion Mutant That Moderates the Repression of Flowering by Cool Temperatures Open
Ambient growing temperature and photoperiod are major environmental stimuli that summer annual crops use to adjust their reproductive phenology so as to maximize yield. Variation in flowering time among soybean (Glycine max) cultiva…
View article: Genetic Consequences of Acute/Chronic Gamma and Carbon Ion Irradiation of Arabidopsis thaliana
Genetic Consequences of Acute/Chronic Gamma and Carbon Ion Irradiation of Arabidopsis thaliana Open
Gamma rays are the most frequently used ionizing radiation in plant mutagenesis; however, few studies are available on the characteristics of mutations at a genome-wide level. Here, we quantitatively and qualitatively characterized the mut…
View article: Molecular Analysis of Carbon Ion-Induced Mutations in DNA Repair-Deficient Strains of Saccharomyces cerevisiae
Molecular Analysis of Carbon Ion-Induced Mutations in DNA Repair-Deficient Strains of Saccharomyces cerevisiae Open
Mutations caused by ion beams have been well-studied in plants, including ornamental flowers, rice, and algae. It has been shown that ion beams have several significantly interesting features, such as a high biological effect and unique mu…
View article: Genome sequencing of ion-beam-induced mutants facilitates detection of candidate genes responsible for phenotypes of mutants in rice
Genome sequencing of ion-beam-induced mutants facilitates detection of candidate genes responsible for phenotypes of mutants in rice Open
Ion beams are physical mutagens used for plant and microbe breeding that cause mutations via a distinct mechanism from those of chemical mutagens or gamma rays. We utilized whole-exome sequencing of rice DNA in order to understand the prop…
View article: Experimental Study on the Biological Effect of Cluster Ion Beams in Bacillus subtilis Spores
Experimental Study on the Biological Effect of Cluster Ion Beams in Bacillus subtilis Spores Open
Cluster ion beams have unique features in energy deposition, but their biological effects are yet to be examined. In this study, we employed bacterial spores as a model organism, established an irradiation method, and examined the lethal e…
View article: Physiological status of plant tissue affects the frequency and types of mutations induced by carbon-ion irradiation in Arabidopsis
Physiological status of plant tissue affects the frequency and types of mutations induced by carbon-ion irradiation in Arabidopsis Open
Ionizing radiation including heavy-ion beams has been widely used in mutation breeding. Dry seeds, seedlings, and cultured tissues are often used for mutagenesis; however, little is known about the differences in induced mutations among th…
View article: Mutation induction of orchid plants by ion beams
Mutation induction of orchid plants by ion beams Open
Mutation induction using ionizing radiation provides an effective alternative for the improvement of orchids. Ion beams were used because they have much higher linear energy transfer (LET) than X-rays and gamma rays subsequently cause high…
View article: Frequent generation of mutants with coincidental changes in multiple traits via ion-beam irradiation in soybean
Frequent generation of mutants with coincidental changes in multiple traits via ion-beam irradiation in soybean Open
Ion beams are powerful mutagens that can induce novel mutants in plants. We previously established a system for producing a mutant population of soybean via ion-beam irradiation, isolated plants that had chlorophyll deficiency, and maintai…