Yosuke Funato
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Phosphatase‐independent role of phosphatase of regenerating liver in cancer progression Open
Phosphatase of regenerating liver (PRL) is a family of protein tyrosine phosphatases (PTPs) that are anchored to the plasma membrane by prenylation. They are frequently overexpressed in various types of malignant cancers and their roles in…
Biological importance and therapeutic potential of Mg<sup>2+</sup> transporter CNNM Open
Cyclin M (CNNM) constitute a family of proteins that function as Mg2+-extruding transporters by stimulating Na+/Mg2+ exchange, and thereby control intracellular Mg2+ levels. This CNNM-mediated Mg2+-extrusion is inhibited by the direct bind…
PRL stimulates mitotic errors by suppressing kinetochore-localized activation of AMPK during mitosis Open
Phosphatase of regenerating liver (PRL) is frequently overexpressed in various malignant cancers and is known to be a driver of malignancy. Here, we demonstrated that PRL overexpression causes mitotic errors that accompany spindle misorien…
A novel role for PRL in regulating epithelial cell density by inducing apoptosis at confluence Open
Maintaining proper epithelial cell density is essential for the survival of multicellular organisms. Although regulation of cell density through apoptosis is well known, its mechanistic details remain elusive. Here, we report the involveme…
The emerging roles and therapeutic potential of cyclin M/CorC family of Mg2+ transporters Open
Cyclin M (CNNM) and its prokaryotic ortholog CorC belong to a family of proteins that function as Mg2+-extruding transporters by stimulating Na+/Mg2+ exchange, and thereby control intracellular Mg2+ levels. The Mg2+-extruding function of C…
Identification and mechanistic analysis of an inhibitor of the CorC Mg2+ transporter Open
The CorC/CNNM family of Na+-dependent Mg2+ transporters is ubiquitously conserved from bacteria to humans. CorC, the bacterial CorC/CNNM family of proteins, is involved in resistance to antibiotic exposure and in the survival of pathogenic…
Identification and mechanistic analysis of an inhibitor of the CorC Mg <sup>2+</sup> transporter Open
The CorC/CNNM family of Na + -dependent Mg 2+ transporters is ubiquitously conserved from bacteria to humans. CorC, the bacterial member of the CorC/CNNM family of proteins, is involved in resistance to antibiotic exposure and in the survi…
Structural basis for the Mg <sup>2+</sup> recognition and regulation of the CorC Mg <sup>2+</sup> transporter Open
Crystal structures of the CorC Mg 2+ transporter revealed the unique Mg 2+ binding site associated with genetic diseases.
View article: Magnesium efflux from Drosophila Kenyon cells is critical for normal and diet-enhanced long-term memory
Magnesium efflux from Drosophila Kenyon cells is critical for normal and diet-enhanced long-term memory Open
Dietary magnesium (Mg 2+ ) supplementation can enhance memory in young and aged rats. Memory-enhancing capacity was largely ascribed to increases in hippocampal synaptic density and elevated expression of the NR2B subunit of the NMDA-type …
View article: Author response: Magnesium efflux from Drosophila Kenyon cells is critical for normal and diet-enhanced long-term memory
Author response: Magnesium efflux from Drosophila Kenyon cells is critical for normal and diet-enhanced long-term memory Open
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Dietary magnesium (Mg2+) supp…
View article: Involvement of I-BAR protein IRSp53 in tumor cell growth via extracellular microvesicle secretion
Involvement of I-BAR protein IRSp53 in tumor cell growth via extracellular microvesicle secretion Open
Cellular protrusions mediated by the membrane-deforming I-BAR domain protein IRSp53 are involved in cell migration, including metastasis. However, the role of IRSp53 in cell proliferation remains unclear. Here, we examined the role of IRSp…
Control of protein function through oxidation and reduction of persulfidated states Open
Thioredoxin system–mediated persulfidation of Cys residues controls protein function and protects them from oxidative stress.
Phosphatase of regenerating liver sensitizes MET to functional activation by hepatocyte growth factor Open
Phosphatase of regenerating liver (PRL) is overexpressed in metastatic cancers and actively drives their malignant progression. Many studies on cultured cancer cells have implied PRL overexpression as a stimulant for cellular signaling inv…
Molecular function and biological importance of CNNM family Mg2+ transporters Open
Cyclin M (CNNM) family proteins are evolutionarily conserved Mg2+ transporters. They extrude Mg2+ from cells and maintain intracellular Mg2+ levels within the normal range. Moreover, they play an important role in Mg2+ (re)absorption in th…
The cyclic nucleotide–binding homology domain of the integral membrane protein CNNM mediates dimerization and is required for Mg2+ efflux activity Open
Proteins of the cyclin M family (CNNMs; also called ancient conserved domain proteins, or ACDPs) are represented by four integral membrane proteins that have been proposed to function as Mg2+ transporters. CNNMs are associated with a numbe…
CrossTalk proposal: CNNM proteins are Na<sup>+</sup>/Mg<sup>2+</sup> exchangers playing a central role in transepithelial Mg<sup>2+</sup> (re)absorption Open
Magnesium (Mg) homeostasis is maintained by both intestinal absorption and renal reabsorption. Commonly, in these processes, Mg2+ is transported from the tubular lumen to the body interior, passing through epithelial sheets. Transcellular …
Rebuttal from Yosuke Funato, Kazuharu Furutani, Yoshihisa Kurachi and Hiroaki Miki Open
Arjona and de Baaij (2018) nicely summarize the history of cyclin M (CNNM) research. However, we do not agree with their conclusion that CNNM proteins are not Na+/Mg2+ exchangers. Their conclusion is primarily based on the following observ…
Visualization of long-term Mg<sup>2+</sup>dynamics in apoptotic cells using a novel targetable fluorescent probe Open
Long-term Mg2+imaging during apoptosis using a HaloTag-coupled Mg2+probe demonstrated a Mg2+concentration increase caused by dissociation of Mg2+from ATP.
Mg2+ Extrusion from Intestinal Epithelia by CNNM Proteins Is Essential for Gonadogenesis via AMPK-TORC1 Signaling in Caenorhabditis elegans Open
Mg2+ serves as an essential cofactor for numerous enzymes and its levels are tightly regulated by various Mg2+ transporters. Here, we analyzed Caenorhabditis elegans strains carrying mutations in genes encoding cyclin M (CNNM) Mg2+ transpo…
The Mg2+ transporter CNNM4 regulates sperm Ca2+ homeostasis and is essential for reproduction Open
Ca2+ influx triggers sperm capacitation; however, the underlying regulatory mechanisms remain incompletely understood. Here, we show that CNNM4, a Mg2+ transporter, is required for Ca2+ influx during capacitation. We find that Cnnm4-defici…