Young Ah Goo
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View article: Deciphering acquired resistance mechanisms to sustained auxin-inducible protein degradation in cells and mice
Deciphering acquired resistance mechanisms to sustained auxin-inducible protein degradation in cells and mice Open
Targeted protein degradation is a favorable strategy for studying the immediate downstream effects of protein loss-of-function. An appealing platform among these technologies is the auxin-inducible degron (AID) system. Although this system…
View article: Exploitable mechanisms of antibody and CAR mediated macrophage cytotoxicity
Exploitable mechanisms of antibody and CAR mediated macrophage cytotoxicity Open
Macrophages infiltrate solid tumors and either support survival or induce cancer cell death through phagocytosis or cytotoxicity. To uncover regulators of macrophage cytotoxicity towards cancer cells, we perform two co-culture CRISPR scree…
View article: Cord blood proteomics identifies biomarkers of early-onset neonatal sepsis
Cord blood proteomics identifies biomarkers of early-onset neonatal sepsis Open
BACKGROUNDSymptoms of early-onset neonatal sepsis (EOS) in preterm infants are nonspecific and overlap with normal postnatal physiological adaptations and noninfectious pathologies. This clinical uncertainty and the lack of reliable EOS di…
View article: An Antibody Biomarker Associated with Onchocerca volvulus Microfilariae Identified by Proteomic Analysis of Parasite Tissues Isolated from Paraffin Embedded O. volvulus Nodules by Laser Capture Microdissection
An Antibody Biomarker Associated with Onchocerca volvulus Microfilariae Identified by Proteomic Analysis of Parasite Tissues Isolated from Paraffin Embedded O. volvulus Nodules by Laser Capture Microdissection Open
Onchocerciasis (river blindness), a neglected tropical disease caused by the filarial nematode Onchocerca volvulus , impacts millions of people in sub-Saharan Africa. The WHO coordinates global efforts to eliminate onchocerciasis and has p…
View article: Advances in Spatial Multi‐Omics: A Review of Multi‐Modal Mass Spectrometry Imaging and Laser Capture Microdissection‐LCMS Integration
Advances in Spatial Multi‐Omics: A Review of Multi‐Modal Mass Spectrometry Imaging and Laser Capture Microdissection‐LCMS Integration Open
Mass spectrometry has long been utilized to characterize a variety of biomolecules such as proteins, metabolites, and lipids. Most MS‐based omics studies rely on bulk analysis; however, bulk approaches often overlook low‐abundance molecule…
View article: Spatial proteomics of Onchocerca volvulus with pleomorphic neoplasms shows local and systemic dysregulation of protein expression
Spatial proteomics of Onchocerca volvulus with pleomorphic neoplasms shows local and systemic dysregulation of protein expression Open
Onchocerca volvulus is the agent of onchocerciasis (river blindness) and targeted by WHO for elimination though mass drug administration with ivermectin. A small percentage of adult female worms develop pleomorphic neoplasms (PN) which occ…
View article: Evolution of the umbilical cord blood proteome across gestational development
Evolution of the umbilical cord blood proteome across gestational development Open
View article: Spatial proteomics of<i>Onchocerca volvulus</i>with pleomorphic neoplasms shows local and systemic dysregulation of protein expression
Spatial proteomics of<i>Onchocerca volvulus</i>with pleomorphic neoplasms shows local and systemic dysregulation of protein expression Open
Onchocerca volvulus is the agent of onchocerciasis (river blindness) and targeted by WHO for elimination though mass drug administration with ivermectin. A small percentage of adult worms develop pleomorphic neoplasms (PN) that are positiv…
View article: Enhanced Staphylococcus aureus protection by uncoupling of the α-toxin-ADAM10 interaction during murine neonatal vaccination
Enhanced Staphylococcus aureus protection by uncoupling of the α-toxin-ADAM10 interaction during murine neonatal vaccination Open
View article: The Impact of Milk on Gut Permeability, Fecal 16S rRNA Gene Microbiota Profiling, and Fecal Metabolomics in Children with Moderate Malnutrition in Sierra Leone: A Double-Blind, Randomized Controlled Trial
The Impact of Milk on Gut Permeability, Fecal 16S rRNA Gene Microbiota Profiling, and Fecal Metabolomics in Children with Moderate Malnutrition in Sierra Leone: A Double-Blind, Randomized Controlled Trial Open
Abnormal intestinal permeability do not improve with 4 wk of supplementary feeding. Fecal rRNA do not differ with consumption of different diets. This trial was registered at clinicaltrials.gov as NCT04216043.
View article: Experimental and Computational Evaluation of Lipidomic In-Source Fragmentation as a Result of Postionization with Matrix-Assisted Laser Desorption/Ionization
Experimental and Computational Evaluation of Lipidomic In-Source Fragmentation as a Result of Postionization with Matrix-Assisted Laser Desorption/Ionization Open
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can provide spatially resolved molecular information about a sample. Recently, a postionization approach (MALDI-2) has been commercially integrated with MALD…
View article: Reduced guanidinoacetate in plasma of patients with autosomal dominant Fanconi syndrome due to heterozygous <scp>P341L <i>GATM</i></scp> variant and study of organoids towards treatment
Reduced guanidinoacetate in plasma of patients with autosomal dominant Fanconi syndrome due to heterozygous <span>P341L <i>GATM</i></span> variant and study of organoids towards treatment Open
Autosomal dominant Fanconi syndrome due to a GATM variant (GATM‐FS), causes accumulation of misfolded arginine‐glycine amidinotransferase (AGAT) in proximal renal tubules leading to cellular injury. GATM‐FS presents during childhood and pr…
View article: Evolution of the Umbilical Cord Blood Proteome Across Gestational Development
Evolution of the Umbilical Cord Blood Proteome Across Gestational Development Open
Neonatal health is dependent on early risk stratification, diagnosis, and timely management of potentially devastating conditions, particularly in the setting of prematurity. Many of these conditions are poorly predicted in real-time by cl…
View article: Supplementary Figure S7 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Supplementary Figure S7 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Supplementary Figure 7. Biophysical characterization of the purified L111 antibody. A. Analysis of purified L111 antibody integrity by non-reduced (top) and reduced (bottom) capillary electrophoresis-SDS (CE-SDS) on a Perkin Elmer LabChip …
View article: Data from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Data from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Purpose:Tax-interacting protein 1 (TIP1) is a cancer-specific radiation-inducible cell surface antigen that plays a role in cancer progression and resistance to therapy. This study aimed to develop a novel anti-TIP1 human antibody for noni…
View article: Supplementary Figure S3 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Supplementary Figure S3 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Supplementary Figure 3. A. Representative chromatogram showing protein A purification profile of the L111 antibody. B. Representative size exclusion chromatogram in S200 10/300 GL columns of the purified L111 antibody.
View article: Supplementary Figure S12 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Supplementary Figure S12 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Supplementary Figure 12. In vitro characterization of [89Zr]Zr-DFO-L111. A. SDS-PAGE under non-reducing and reducing conditions for the [89Zr]Zr-DFO-L111 developed by autoradiography. Four two-fold dilutions were loaded on the SDS-PAGE gel…
View article: Supplementary Figure S9 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Supplementary Figure S9 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Supplementary Figure 9. A. HPLC-SEC analysis of L111 and L111-DFO on an Agilent 1200 series HPLC system using a Zorbax GF-250 9.4 mm ID x 25 cm column (Agilent). DFO conjugation did not change the retention time of the antibody, and no agg…
View article: Supplementary Table S1 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Supplementary Table S1 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Supplementary Table 1. Rank ordering of the anti-TIP1 antibodies based on their KD values
View article: Supplementary Figure S13 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Supplementary Figure S13 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Supplementary Figure 13. A. Schematic representation of the tumor implantation, irradiation, antibody injection, PET imaging and post-PET biodistribution schedule. B and C. The tumor SUV max in H460 tumors (B) and A549 tumors (C) at days 2…
View article: Supplementary Figure S10 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Supplementary Figure S10 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Supplementary Figure 10. A. In vitro stability of [89Zr]Zr-DFO-L111 antibodies that were either freshly conjugated with DFO or thawed from -80°C after DFO conjugation. The stability was assessed in human serum by instant thin-layer chromat…
View article: Supplementary Figure S1 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Supplementary Figure S1 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Supplementary Figure 1. Strategy for WashU II phage display library creation. The human antibody repertoires were amplified by PCR from the cDNA of mixed human peripheral blood mononuclear cells and cloned randomly into a phagemid vector. …
View article: Supplementary Figure S6 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Supplementary Figure S6 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Supplementary Figure 6. Flow cytometry evaluation of the purified anti-TIP1 antibodies on H460 (A) and A549 (B) cells. The percentage of anti-TIP1+ vs. antibody concentration was plotted in GraphPad Prism software using One Site-specific b…
View article: Supplementary Figure S8 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Supplementary Figure S8 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Supplementary Figure 8. DFO-to-L111 ratio (DAR) analysis by native MS. The deconvoluted mass spectra (deconvoluted using Intact Mass, Protein Metrics Inc) show the increasing numbers of DFO (+1 to +8). The respective DARs are shown in red.
View article: Supplementary Figure S13 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Supplementary Figure S13 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Supplementary Figure 13. A. Schematic representation of the tumor implantation, irradiation, antibody injection, PET imaging and post-PET biodistribution schedule. B and C. The tumor SUV max in H460 tumors (B) and A549 tumors (C) at days 2…
View article: Supplementary Figure S11 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Supplementary Figure S11 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Supplementary Figure 11. Specific uptake of the [89Zr]Zr-DFO-L111 in tumors in vivo. Bar graph showing the uptake of the [89Zr]Zr-DFO-Isotype or [89Zr]Zr-DFO-L111 at days 2 and 5 in nude mice bearing A549 tumors in the hindlimb (n=4). Tumo…
View article: Supplementary Table S2 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Supplementary Table S2 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Supplementary Table 2. The analysis of the DFO-to-antibody ratio for the D10-L111 by native mass spectrometry
View article: Supplementary Figure S12 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Supplementary Figure S12 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Supplementary Figure 12. In vitro characterization of [89Zr]Zr-DFO-L111. A. SDS-PAGE under non-reducing and reducing conditions for the [89Zr]Zr-DFO-L111 developed by autoradiography. Four two-fold dilutions were loaded on the SDS-PAGE gel…
View article: Supplementary Table S3 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Supplementary Table S3 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Supplementary Table 3. Rank ordering of the DFO conjugated L111 antibodies based on their KD values
View article: Supplementary Figure S11 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library
Supplementary Figure S11 from Development of a [<sup>89</sup>Zr]Zr-labeled Human Antibody using a Novel Phage-displayed Human scFv Library Open
Supplementary Figure 11. Specific uptake of the [89Zr]Zr-DFO-L111 in tumors in vivo. Bar graph showing the uptake of the [89Zr]Zr-DFO-Isotype or [89Zr]Zr-DFO-L111 at days 2 and 5 in nude mice bearing A549 tumors in the hindlimb (n=4). Tumo…