Explanipedia

Bipartite Genetically Encoded Biosensors to Sense Calcium Ion Dynamics at Membrane–Membrane Contact Sites Open

Isamu Yamaguchi, Lucia Barazzuol, Giulia Dematteis, Wenchao Zhu, Yurong Wen , et al. · 2025

Self-complementing bipartite fluorescent proteins (FPs) are useful tools for the detection of protein-protein proximity and for localizing fluorophores to membrane-membrane contact sites. Here, we report versions of circularly permuted gre…

Structural and mechanistic insights into the transcriptional regulation of chromosomal T6SS by large conjugative plasmid-encoded TetRs in <i>Acinetobacter baumannii</i> Open

Wenbo He, Zhenlin Ouyang, Jiaxin Zhang, Xiaolong Guo, Min Jiao , et al. · 2025

The type VI secretion system (T6SS) is a highly sophisticated nanomachine widely used by bacteria to achieve competitive advantage, important for survival and pathogenicity. In Acinetobacter baumannii, the large conjugative plasmid pAB3 en…

The developments and emerging trends of Autonomic Nervous System Research in Arrhythmia: a bibliometric study from 2004 to 2024 Open

Tingting Chen, Yanfeng Yang, Kun Shi, Feifei Si, Yurong Wen , et al. · 2025

The role of the autonomic nervous system in cardiovascular diseases has increasingly attracted the attention of researchers. This study aims to review research on the autonomic nervous system in arrhythmias from 2004 to 2024, with a focus …

Molybdate uptake interplay with ROS tolerance modulates bacterial pathogenesis Open

Meng Jiao, Wenbo He, Zhenlin Ouyang, Qinyue Yu, Jiaxin Zhang , et al. · 2025

The rare metal element molybdenum functions as a cofactor in molybdoenzymes that are essential to life in almost all living things. Molybdate can be captured by the periplasmic substrate-binding protein ModA of ModABC transport system in b…

Combination of single-source dual-energy computed tomography (CT) parameters and extracellular volume fraction for predicting lymphovascular and perineural invasion in colorectal cancer Open

Yuting Zhang, Yurong Wen, Shenglin Li, Liangna Deng, Tao Han , et al. · 2024

ssDECT-derived parameters and ECV fraction may serve as non-invasive tools for predicting the LVI/PNI status in CRC.

Single domain antibody: Development and application in biotechnology and biopharma Open

Ting Yu, Fang Zheng, Wenbo He, Serge Muyldermans, Yurong Wen · 2024

Summary Heavy‐chain antibodies (HCAbs) are a unique type of antibodies devoid of light chains, and comprised of two heavy chains‐only that recognize their cognate antigen by virtue of a single variable domain also referred to as VHH, singl…

Isolation, identification and genetic variation analysis of pseudorabies virus mutant strain from Chinese pig farm Open

Boping Zhou, Lingling Wang, C. Xin, Lin Ji, Shengzhang Dong , et al. · 2024

Pseudorabies (PR) is an important infectious disease affecting pig farms worldwide. In this study, we reported a pseudorabies virus (PRV) isolated from a Bartha-K61-vaccinated pig farm in Linyi, Shandong Province, China. Evidence from viru…

Mechanistic and biophysical characterization of polymyxin resistance response regulator PmrA in Acinetobacter baumannii Open

Zhenlin Ouyang, Wenbo He, Min Jiao, Qinyue Yu, Yucheng Guo , et al. · 2024

Introduction Acinetobacter baumannii PmrAB is a crucial two-component regulatory system (TCS) that plays a vital role in conferring resistance to polymyxin. PmrA, a response regulator belonging to the OmpR/PhoB family, is composed of a C-t…

Rational Engineering of an Improved Genetically Encoded pH Sensor Based on Superecliptic pHluorin Open

Yi Shen, Yurong Wen, Silvia Sposini, Anjali Amrapali Vishwanath, Ahmed S. Abdelfattah , et al. · 2023

Genetically encoded pH sensors based on fluorescent proteins are valuable tools for the imaging of cellular events that are associated with pH changes, such as exocytosis and endocytosis. Superecliptic pHluorin (SEP) is a pH-sensitive gree…

Structure and assembly of type VI secretion system cargo delivery vehicle Open

Wenbo He, Ke Wu, Zhenlin Ouyang, Yixin Bai, Wen Luo , et al. · 2023

Type VI secretion system is widely used in Gram-negative bacteria for injecting toxic effectors into neighboring prokaryotic or eukaryotic cells. Various effectors can be loaded onto the T6SS delivery tube via its core components: Hcp, Vgr…

Screening and epitope characterization of diagnostic nanobody against total and activated Bacteroides fragilis toxin Open

Yu-cheng Guo, Zhenlin Ouyang, Wenbo He, Jiaxin Zhang, Qian Qin , et al. · 2023

Enterotoxigenic Bacteroides fragilis (ETBF) can rapidly secrete an enterotoxin termed B. fragilis toxin (BFT), which is thought to be the only recognized virulence factor in ETBF. ETBF can cause acute diarrhea, inflammatory bowel disease (…

Structure and activation mechanism of Staphylococcus aureus response regulator ArlR by solution NMR Open

M. Rafid Feisal, Philip B. Liu, Yurong Wen, Peter M. Hwang · 2023

Efficacy of immune checkpoint inhibitors along with chemotherapy in non-small cell lung cancer and the impact on adverse reactions and serum tumor markers. Open

Miao Xie, Xinlin Wang, Peng Wang, An Liu, Yurong Wen , et al. · 2023

For NSCLC patients, the adoption of PD-1/PD-L1 inhibitors following chemoradiotherapy shows potential in enhancing clinical efficacy, boosting patients' immune function, and improving long-term survival rates, with premising safety profile.

Single Domain Antibody application in bacterial infection diagnosis and neutralization Open

Qian Qin, Hao Liu, Wenbo He, Yucheng Guo, Jiaxin Zhang , et al. · 2022

Increasing antibiotic resistance to bacterial infections causes a serious threat to human health. Efficient detection and treatment strategies are the keys to preventing and reducing bacterial infections. Due to the high affinity and antig…

Progress in structural and functional study of the bacterial phenylacetic acid catabolic pathway, its role in pathogenicity and antibiotic resistance Open

Min Jiao, Wenbo He, Zhenlin Ouyang, Qindong Shi, Yurong Wen · 2022

Phenylacetic acid (PAA) is a central intermediate metabolite involved in bacterial degradation of aromatic components. The bacterial PAA pathway mainly contains 12 enzymes and a transcriptional regulator, which are involved in biofilm form…

A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life Open

Sheng-Yi Wu, Yurong Wen, Nelson BC Serre, Cathrine Charlotte Heiede Laursen, Andrea Dietz , et al. · 2022

Potassium ion (K + ) plays a critical role as an essential electrolyte in all biological systems. Genetically-encoded fluorescent K + biosensors are promising tools to further improve our understanding of K + -dependent processes under nor…

Correction to: Prostate-specific oncogene OTUD6A promotes prostatic tumorigenesis via deubiquitinating and stabilizing c-Myc Open

Yunhua Peng, Jing Liu, Zhen Wang, Chun‐Ping Cui, Tiantian Zhang , et al. · 2022

An Outer Membrane Protein YiaD Contributes to Adaptive Resistance of Meropenem in Acinetobacter baumannii Open

Lei Han, Yiyuan Gao, Yuqing Liu, Siyu Yao, Shuyan Zhong , et al. · 2022

Acinetobacter baumannii can develop various resistance mechanisms to carbapenems. However, the factors involved in the evolutionary process that leads from transition to the sensitive to resistant phenotype are not clear.

Prostate-specific oncogene OTUD6A promotes prostatic tumorigenesis via deubiquitinating and stabilizing c-Myc Open

Yunhua Peng, Jing Liu, Zhen Wang, Chun‐Ping Cui, Tiantian Zhang , et al. · 2022

A genetically encoded fluorescent biosensor for extracellular l-lactate Open

Yusuke Nasu, Ciaran Murphy‐Royal, Yurong Wen, Jordan N. Haidey, Rosana S. Molina , et al. · 2021

Characterization of Amino Acid Substitutions in the Two-Component Regulatory System AdeRS Identified in Multidrug-Resistant Acinetobacter baumannii Open

Kai Lucaßen, Kyriaki Xanthopoulou, Julia Wille, Thorsten Wille, Yurong Wen , et al. · 2021

The active efflux of antimicrobials by bacteria can lead to antimicrobial resistance and persistence and can affect multiple different classes of antimicrobials. Efflux pumps are tightly regulated, and their overexpression can be mediated …

A sensitive and specific genetically encodable biosensor for potassium ions Open

Sheng-Yi Wu, Yurong Wen, Nelson Bernard Calixte Serre, Cathrine Charlotte Heiede Laursen, Andrea Dietz , et al. · 2021

Potassium ions (K + ) play a critical role as an essential electrolyte in all biological systems. Here we report the crystal structure-guided optimization and directed evolution of an improved genetically encoded fluorescent K + biosensor,…

Non-canonical phosphorylation of Bmf by p38 MAPK promotes its apoptotic activity in anoikis Open

Zhe Zhi, Zhenlin Ouyang, Yibo Ren, Ying Cheng, Peijun Liu , et al. · 2021

Proteolysis and multimerization regulate signaling along the two-component regulatory system AdeRS Open

Zhenlin Ouyang, Fang Zheng, Li Zhu, Jan Félix, Di Wu , et al. · 2021

A genetically encoded fluorescent biosensor for extracellular L-lactate Open

Yusuke Nasu, Ciaran Murphy‐Royal, Yurong Wen, Jordan N. Haidey, M. Rosana S. Molina , et al. · 2021

To enable investigations of the emerging roles of cell-to-cell shuttling of L-lactate, we have developed an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. We demonstrate that this biosensor, des…

Development and Characterization of Nanobodies Targeting the Kupffer Cell Open

Fang Zheng, Jinhong Zhou, Zhenlin Ouyang, Jiaxin Zhang, Xinyi Wang , et al. · 2021

Nanobodies that are derived from single-chain antibodies of camelids have served as powerful tools in diagnostics, therapeutics and investigation of membrane receptors' structure and function. In this study, we developed a series of nanobo…

Photocleavable proteins that undergo fast and efficient dissociation Open

Xiaocen Lu, Yurong Wen, Shuce Zhang, Wei Zhang, Yilun Chen , et al. · 2021

Photocleavable proteins can enable the light-dependent modulation of biomolecular activities with high spatiotemporal precision.

Improved Photocleavable Proteins with Faster and More Efficient Dissociation Open

Xiaocen Lu, Yurong Wen, Shuce Zhang, Wei Zhang, Yilun Chen , et al. · 2020

The photocleavable protein (PhoCl) is a green-to-red photoconvertible fluorescent protein that, when illuminated with violet light, undergoes main chain cleavage followed by spontaneous dissociation of the resulting fragments. The first ge…

The distribution of mutations and hotspots in transcription regulators of resistance-nodulation-cell division efflux pumps in tigecycline non-susceptible Acinetobacter baumannii in China Open

Qingye Xu, Xiaoting Hua, Jintao He, Di Zhang, Qiong Chen , et al. · 2020

CIC Is a Mediator of the ERK1/2-DUSP6 Negative Feedback Loop Open

Yibo Ren, Zhenlin Ouyang, Zhanwu Hou, Yuwei Yan, Zhe Zhi , et al. · 2020

DUSP6 functions as an important negative feedback component of the MAPK/ERK signaling pathway. Although DUSP6 expression is tightly regulated by ERK1/2 signaling, the molecular mechanism of this regulation remains partially understood. In …

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