Yuko Takagi
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View article: Nanoscale Surface Metal-Coating Method without Pretreatment for High-Magnification Biological Observation and Applications
Nanoscale Surface Metal-Coating Method without Pretreatment for High-Magnification Biological Observation and Applications Open
Biospecimen imaging is essential across various fields. In particular, a considerable amount of research has focused on developing pretreatment techniques, ranging from freeze-drying to the use of highly conductive polymers, and on advance…
View article: Storage Stability of Nitrite in Nitrite Analysis of Meat Products
Storage Stability of Nitrite in Nitrite Analysis of Meat Products Open
For the analysis of nitrite ions in food, the stabilities of nitrite ions in meat products and their standard solutions were evaluated. Nitrite is easily oxidized or reduced; hence, products with standard solutions or colour retention agen…
View article: Genetic and Functional Analyses of Archaeal ATP-Dependent RNA Ligase in C/D Box sRNA Circularization and Ribosomal RNA Processing
Genetic and Functional Analyses of Archaeal ATP-Dependent RNA Ligase in C/D Box sRNA Circularization and Ribosomal RNA Processing Open
RNA ligases play important roles in repairing and circularizing RNAs post-transcriptionally. In this study, we generated an allelic knockout of ATP-dependent RNA ligase (Rnl) in the hyperthermophilic archaeon Thermococcus kodakarensis to i…
View article: Study of Rapid HPLC Method for the Determination of Antioxidants in Foods, and Standard Solution Stability during Storage
Study of Rapid HPLC Method for the Determination of Antioxidants in Foods, and Standard Solution Stability during Storage Open
In this study, we simultaneously determined three antioxidants, butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), and tert-butylhydroquinone (TBHQ), using HPLC equipped both with a photodiode array detector and a fluorescence…
View article: Differentiating Trypanosoma cruzi in a Host Mammalian Cell Imaged in Aqueous Liquid by Atmospheric Scanning Electron Microscopy
Differentiating Trypanosoma cruzi in a Host Mammalian Cell Imaged in Aqueous Liquid by Atmospheric Scanning Electron Microscopy Open
Using Atmospheric Scanning Electron Microscopy (ASEM), we visualized interaction between infectious stage of Trypanosoma cruzi and completely intact host mammalian cell. Plasma membrane appears translucent under ASEM, which not only enable…
View article: Crystal structures of the RNA triphosphatase from Trypanosoma cruzi provide insights into how it recognizes the 5′-end of the RNA substrate
Crystal structures of the RNA triphosphatase from Trypanosoma cruzi provide insights into how it recognizes the 5′-end of the RNA substrate Open
View article: Utilization of proliferable extracellular amastigotes for transient gene expression, drug sensitivity assay, and CRISPR/Cas9-mediated gene knockout in Trypanosoma cruzi
Utilization of proliferable extracellular amastigotes for transient gene expression, drug sensitivity assay, and CRISPR/Cas9-mediated gene knockout in Trypanosoma cruzi Open
Trypanosoma cruzi has three distinct life cycle stages; epimastigote, trypomastigote, and amastigote. Amastigote is the replication stage in host mammalian cells, hence this stage of parasite has clinical significance in drug development r…
View article: Development of a Motion-Based Cell-Counting System for <i>Trypanosoma</i> Parasite Using a Pattern Recognition Approach
Development of a Motion-Based Cell-Counting System for <i>Trypanosoma</i> Parasite Using a Pattern Recognition Approach Open
Automated cell counters that utilize still images of sample cells are widely used. However, they are not well suited to counting slender, aggregate-prone microorganisms such as Trypanosoma cruzi. Here, we developed a motion-based cell-coun…
View article: Crystal structures of U6 snRNA-specific terminal uridylyltransferase
Crystal structures of U6 snRNA-specific terminal uridylyltransferase Open
View article: Nanomolar Inhibitors of Trypanosoma brucei RNA Triphosphatase
Nanomolar Inhibitors of Trypanosoma brucei RNA Triphosphatase Open
Eukaryal taxa differ with respect to the structure and mechanism of the RNA triphosphatase (RTPase) component of the mRNA capping apparatus. Protozoa, fungi, and certain DNA viruses have a metal-dependent RTPase that belongs to the triphos…
View article: Multitarget, quantitative nanoplasmonic electrical field-enhanced resonating device (NE <sup>2</sup> RD) for diagnostics
Multitarget, quantitative nanoplasmonic electrical field-enhanced resonating device (NE <sup>2</sup> RD) for diagnostics Open
Significance Biosensing technologies have significant impact on medical diagnostics but difficulties in the handling of complex biospecimens, portability, and nonlinearity in dynamic detection range present considerable technical bottlenec…
View article: The messenger RNA decapping and recapping pathway in <i>Trypanosoma</i>
The messenger RNA decapping and recapping pathway in <i>Trypanosoma</i> Open
Significance The 5′ end of eukaryotic mRNA is capped and methylated to protect mRNA from degradation and enhance protein synthesis. However, the cap can be removed from mRNA by decapping. We identified a recapping enzyme with 5′-monophosph…