Explanipedia

Less-deformable erythrocyte subpopulations biomechanically induce endothelial inflammation in sickle cell disease Open

Christina Caruso, Xiaopo Cheng, Marina E. Michaud, Hannah Szafraniec, Beena Thomas , et al. · 2024

Sickle cell disease (SCD) is canonically characterized by reduced red blood cell (RBC) deformability, leading to microvascular obstruction and inflammation. Although the biophysical properties of sickle RBCs are known to influence SCD vasc…

PB0814 Endothelial Cells, Not Blood, Dictate Where, When, and How Hemostasis Initiates under Flow Conditions: Mechanistic Studies Using a Vascularized Hemostasis-on-a-Chip System Open

Yumiko Sakurai, E. Hardy, Iris van Moort, Ruben Bierings, Yijie Qiu , et al. · 2023

Aims:The aim was to study the impact of ABO genotyping on VWD diagnostic on top of the VWF sequence variant in a selected population of VWD3C.Methods: Using Next Generation Sequencing, we genotyped 102 VWD3C (24 ± 14years) included in the …

HTRS2023.O6A.1 Leveraging quantitative platelet adhesion and platelet geometry-sensing assays to evaluate platelet function in type 1 von Willebrand disease Open

Sally Azer, Oluwamayokun Oshinowo, Yumiko Sakurai, Megan Brown, Meredith E. Fay , et al. · 2023

Multiplatform analyses reveal distinct drivers of systemic pathogenesis in adult versus pediatric severe acute COVID-19 Open

Samuel Druzak, Elizabeth Iffrig, Blaine R. Roberts, Tiantian Zhang, Kirby S. Fibben , et al. · 2023

Hemostasis-on-a-chip / incorporating the endothelium in microfluidic models of bleeding Open

Yumiko Sakurai, Elaissa T. Hardy, Wilbur A. Lam · 2023

Currently, point-of-care assays for human platelet function and coagulation are used to assess bleeding risks and drug testing, but they lack intact endothelium, a critical component of the human vascular system. Within these assays, the a…

Membrane curvature and PS localize coagulation proteins to filopodia and retraction fibers of endothelial cells Open

Christopher V. Carman, Dessislava N. Nikova, Yumiko Sakurai, Jialan Shi, Valerie A. Novakovic , et al. · 2022

Prior reports indicate that the convex membrane curvature of phosphatidylserine (PS)-containing vesicles enhances formation of binding sites for factor Va and lactadherin. Yet, the relationship of convex curvature to localization of these …

Multiplatform Analyses Reveal Distinct Drivers of Systemic Pathogenesis in Adult Versus Pediatric COVID-19 Open

Samuel Druzak, Elizabeth Iffrig, Blaine R. Roberts, Tiantian Zhang, Anne M. Roberts , et al. · 2022

Platelet heterogeneity enhances blood clot volumetric contraction: An example of asynchrono-mechanical amplification Open

Yueyi Sun, David R. Myers, Svetoslav Nikolov, Oluwamayokun Oshinowo, John Baek , et al. · 2021

Correction: A novel mutation in the SLCO2A1 gene, encoding a prostaglandin transporter, induces chronic enteropathy Open

Keisuke Jimbo, Toshiaki Okuno, Ryuichi Ohgaki, Kou Nishikubo, Yuri Kitamura , et al. · 2021

[This corrects the article DOI: 10.1371/journal.pone.0241869.].

Significant differences in single-platelet biophysics exist across species but attenuate during clot formation Open

Oluwamayokun Oshinowo, Renee Copeland, Yumiko Sakurai, Meredith E. Fay, Brian G. Petrich , et al. · 2021

Key Points Human, canine, ovine, and porcine platelets exhibit disparate biophysical signatures, whereas human and murine platelets are similar. Multiple biophysical parameters integrate during clot formation, measured by bulk clot contrac…

A novel mutation in the SLCO2A1 gene, encoding a prostaglandin transporter, induces chronic enteropathy Open

Keisuke Jimbo, Toshiaki Okuno, Ryuichi Ohgaki, Kou Nishikubo, Tetsuhisa Kitamura , et al. · 2020

Chronic enteropathy associated with SLCO2A1 gene (CEAS) is caused by loss-of-function mutations in SLCO2A1 , which encodes a prostaglandin (PG) transporter. In this study, we report a sibling case of CEAS with a novel pathogenic variant of…

Getting a good view:<i>in vitro</i>imaging of platelets under flow Open

Oluwamayokun Oshinowo, Tamara P. Lambert, Yumiko Sakurai, Renee Copeland, Caroline E. Hansen , et al. · 2020

As the anucleate cells responsible for hemostasis and thrombosis, platelets are exposed to a myriad of biophysical and biochemical stimuli within vasculature and heterogeneous blood clots. Highly controlled, reductionist in vitro im…

The CFTR gene variants in Japanese children with idiopathic pancreatitis Open

Manami Iso, Mitsuyoshi Suzuki, Kumiko Yanagi, Kei Minowa, Yumiko Sakurai , et al. · 2019

The cystic fibrosis transmembrane conductance regulator ( CFTR ) gene has been reported as one of the pancreatitis susceptibility genes. Although many variants of CFTR have been reported in Caucasian patients, there are few data in Japanes…

Microvasculature-on-a-chip for the long-term study of endothelial barrier dysfunction and microvascular obstruction in disease Open

Yongzhi Qiu, Byungwook Ahn, Yumiko Sakurai, Caroline E. Hansen, Reginald Tran , et al. · 2018

A microengineered vascularized bleeding model that integrates the principal components of hemostasis Open

Yumiko Sakurai, Elaissa T. Hardy, Byungwook Ahn, Reginald Tran, Meredith E. Fay , et al. · 2018

Extracellular fluid tonicity impacts sickle red blood cell deformability and adhesion Open

Marcus A. Carden, Meredith E. Fay, Xinran Lu, Robert G. Mannino, Yumiko Sakurai , et al. · 2017

Key Points Intravenous fluids are used when treating VOE, but guidelines are lacking, and how IVF tonicity affects sickle red cell biomechanics is unknown. Modifying extracellular fluid tonicity alters deformability, adhesivity, and occlus…

Microfluidic Transduction Harnesses Mass Transport Principles to Enhance Gene Transfer Efficiency Open

Reginald Tran, David R. Myers, Gabriela Denning, Jordan E Shields, Allison M. Lytle , et al. · 2017

Magnetic forces enable controlled drug delivery by disrupting endothelial cell-cell junctions Open

Yongzhi Qiu, Sheng Tong, Linlin Zhang, Yumiko Sakurai, David R. Myers , et al. · 2017

Single-platelet nanomechanics measured by high-throughput cytometry Open

David R. Myers, Yongzhi Qiu, Meredith E. Fay, Michael Tennenbaum, Daniel Chester , et al. · 2016

Genetic Analysis of Japanese Children With Acute Recurrent and Chronic Pancreatitis Open

Nobutomo Saito, Mitsuyoshi Suzuki, Yumiko Sakurai, Satoshi Nakano, Nakayuki Naritaka , et al. · 2016

Objectives: Causes of acute recurrent pancreatitis (ARP) or chronic pancreatitis (CP) are sometimes difficult to determine in children. In such patients, genetic analysis may prove helpful. The present study analyzed mutations of cationic …

Cellular softening mediates leukocyte demargination and trafficking, thereby increasing clinical blood counts Open

Meredith E. Fay, David R. Myers, Amit Kumar, Cory Turbyfield, Rebecca Byler , et al. · 2016

Significance Clinical hematologists have long known that antiinflammatory glucocorticoids such as dexamethasone and blood pressure-supporting catecholamines such as epinephrine cause leukocytes to demarginate from the vascular wall and mic…

Platelet geometry sensing spatially regulates α-granule secretion to enable matrix self-deposition Open

Yumiko Sakurai, Jennifer L. Fitch-Tewfik, Yongzhi Qiu, Byungwook Ahn, David R. Myers , et al. · 2015

Key Points The geometric orientation of the underlying matrix regulates platelet α-granule secretion. On geometrically constrained matrices, platelets self-deposit additional matrix, providing more cell membrane to extend spreading.

Resolving the multifaceted mechanisms of the ferric chloride thrombosis model using an interdisciplinary microfluidic approach Open

Jordan C. Ciciliano, Yumiko Sakurai, David R. Myers, Meredith E. Fay, Béatrice Hechler , et al. · 2015

Key Points The multivariate mechanism of FeCl3-induced thrombosis is rooted in colloidal chemistry, mass transfer, and biological clotting. FeCl3-induced thrombosis is mediated by charge-based binding of proteins (cell surface bound and so…

Platelet Mechanosensing of Collagen Matrices Open

Matthew F. Kee, David R. Myers, Yumiko Sakurai, Wilbur A. Lam, Yongzhi Qiu · 2015

During vascular injury, platelets adhere to exposed subendothelial proteins, such as collagen, on the blood vessel walls to trigger clot formation. Although the biochemical signalings of platelet-collagen interactions have been well charac…

Our Strategy for Fingertip Amputation Open

H Mogami, Yumiko Sakurai · 2015

Twenty-one patients were comprised of 16 males, 5 females, aged one to 77 (average age 36.0).Amputation levels were as follows: subzone I, 10 fingers; subzone II, 7 fingers; subzone III, 1 finger; and subzone IV, 4 fingers (Table 3).Type o…

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