Yuncheng Man
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View article: Synergistic therapeutic effects of endolysin BNT331 and a <i>Lactobacillus crispatus</i> consortium in a human vagina chip model of bacterial vaginosis
Synergistic therapeutic effects of endolysin BNT331 and a <i>Lactobacillus crispatus</i> consortium in a human vagina chip model of bacterial vaginosis Open
Bacterial vaginosis (BV) is a vaginal infection caused by an imbalance in the vaginal microbiome characterized by a decrease in healthy bacteria dominated by Lactobacillus crispatus along with a concomitant increase in dysbiotic bacteria, …
View article: Endothelial CLK2 as a therapeutic target for acute radiation syndrome
Endothelial CLK2 as a therapeutic target for acute radiation syndrome Open
Damage to the vascular endothelium is a major contributor to acute radiation injury in multiple organs that underlies acute radiation syndrome (ARS), yet there are no FDA-approved radiation countermeasures targeting endothelial cells. Use …
View article: New sickle cell disease therapies modulate red blood cell deformability via Ca2+, ATP, and 2,3-DPG in normoxia and hypoxia
New sickle cell disease therapies modulate red blood cell deformability via Ca2+, ATP, and 2,3-DPG in normoxia and hypoxia Open
View article: <i>Ex vivo</i> recapitulation of intramuscular mRNA vaccination with naïve and recall antigens using a human Lymphoid Follicle Chip platform
<i>Ex vivo</i> recapitulation of intramuscular mRNA vaccination with naïve and recall antigens using a human Lymphoid Follicle Chip platform Open
Summary Predicting the efficacy and toxicity of intramuscular mRNA vaccines remains challenging. Here, we describe an ex vivo human cell-based model that replicates immune responses to lipid nanoparticle (LNP)-based mRNA vaccines that requ…
View article: Host Serine Proteases and Antiviral Innate Immunity as Potential Therapeutic Targets in Influenza A Virus Infection-Induced COPD Exacerbations
Host Serine Proteases and Antiviral Innate Immunity as Potential Therapeutic Targets in Influenza A Virus Infection-Induced COPD Exacerbations Open
Lung manifestations of chronic obstructive pulmonary disease (COPD) are often exacerbated by influenza A virus infections; however, the underlying mechanisms remain largely unknown, and hence therapeutic options are limited. Using a physio…
View article: Preclinical assessment of pan-influenza A virus CRISPR RNA therapeutics in a human lung alveolus chip
Preclinical assessment of pan-influenza A virus CRISPR RNA therapeutics in a human lung alveolus chip Open
Human Organ Chips as a useful preclinical tool for rapid efficacy and safety assessment of pan-influenza antiviral CRISPR RNA therapeutics for combating future influenza pandemics.
View article: Exacerbation of influenza virus induced lung injury by alveolar macrophages and its suppression by pyroptosis blockade in a human lung alveolus chip
Exacerbation of influenza virus induced lung injury by alveolar macrophages and its suppression by pyroptosis blockade in a human lung alveolus chip Open
Alveolar macrophages (AMs) are the major sentinel immune cells in human alveoli and play a central role in eliciting host inflammatory responses upon distal lung viral infection. Here, we incorporated peripheral human monocyte-derived macr…
View article: Motion blur microscopy: in vitro imaging of cell adhesion dynamics in whole blood flow
Motion blur microscopy: in vitro imaging of cell adhesion dynamics in whole blood flow Open
Imaging and characterizing the dynamics of cellular adhesion in blood samples is of fundamental importance in understanding biological function. In vitro microscopy methods are widely used for this task but typically require diluting the b…
View article: Beta-defensin index: A functional biomarker for oral cancer detection
Beta-defensin index: A functional biomarker for oral cancer detection Open
There is an unmet clinical need for a non-invasive and cost-effective test for oral squamous cell carcinoma (OSCC) that informs clinicians when a biopsy is warranted. Human beta-defensin 3 (hBD-3), an epithelial cell-derived anti-microbial…
View article: Rapid measurement of hemoglobin-oxygen dissociation by leveraging Bohr effect and Soret band bathochromic shift
Rapid measurement of hemoglobin-oxygen dissociation by leveraging Bohr effect and Soret band bathochromic shift Open
We present a rapid hemoglobin-oxygen dissociation measurement approach to screen for and detect hemoglobin-oxygen affinity abnormalities and to evaluate the function and efficacy of hemoglobin modifying therapies within minutes.
View article: A human lung alveolus-on-a-chip model of acute radiation-induced lung injury
A human lung alveolus-on-a-chip model of acute radiation-induced lung injury Open
View article: Motion Blur Microscopy
Motion Blur Microscopy Open
Imaging and characterizing the dynamics of cellular adhesion in blood samples is of fundamental importance in understanding biological function. In vitro microscopy methods are widely used for this task, but typically require diluting the …
View article: Catch bonds in sickle cell disease: Shear-enhanced adhesion of red blood cells to laminin
Catch bonds in sickle cell disease: Shear-enhanced adhesion of red blood cells to laminin Open
View article: Membrane bending and sphingomyelinase-associated, sulfatide-dependent hypoxic adhesion of sickle mature erythrocytes
Membrane bending and sphingomyelinase-associated, sulfatide-dependent hypoxic adhesion of sickle mature erythrocytes Open
Abnormal erythrocyte adhesion owing to polymerization of sickle hemoglobin is central to the pathophysiology of sickle cell disease (SCD). Mature erythrocytes constitute >80% of all erythrocytes in SCD; however, the relative contributio…
View article: Sickle red blood cell‐derived extracellular vesicles activate endothelial cells and enhance sickle red cell adhesion mediated by von Willebrand factor
Sickle red blood cell‐derived extracellular vesicles activate endothelial cells and enhance sickle red cell adhesion mediated by von Willebrand factor Open
Summary Endothelial activation and sickle red blood cell (RBC) adhesion are central to the pathogenesis of sickle cell disease (SCD). Quantitatively, RBC‐derived extracellular vesicles (REVs) are more abundant from SS RBCs compared with he…
View article: Beta Defensin Index (BDI): A Novel Biomarker for Oral Cancer Detection
Beta Defensin Index (BDI): A Novel Biomarker for Oral Cancer Detection Open
View article: Microfluidic concurrent assessment of red blood cell adhesion and microcapillary occlusion: potential hemorheological biomarkers in sickle cell disease
Microfluidic concurrent assessment of red blood cell adhesion and microcapillary occlusion: potential hemorheological biomarkers in sickle cell disease Open
A microfluidic assay integrated with capillary network-inspired microcapillary arrays and endothelial-associated protein functionalization enables concurrent assessment of red blood cell adhesion and microcapillary occlusion.
View article: Multispectral imaging for MicroChip electrophoresis enables point-of-care newborn hemoglobin variant screening
Multispectral imaging for MicroChip electrophoresis enables point-of-care newborn hemoglobin variant screening Open
Hemoglobin (Hb) disorders affect nearly 7% of the world's population. Globally, around 400,000 babies are born annually with sickle cell disease (SCD), primarily in sub-Saharan Africa where morbidity and mortality rates are high. Screening…
View article: A microfluidic device for assessment of E-selectin-mediated neutrophil recruitment to inflamed endothelium and prediction of therapeutic response in sickle cell disease
A microfluidic device for assessment of E-selectin-mediated neutrophil recruitment to inflamed endothelium and prediction of therapeutic response in sickle cell disease Open
View article: Catch bonds in sickle cell disease: shear-enhanced adhesion of red blood cells to laminin
Catch bonds in sickle cell disease: shear-enhanced adhesion of red blood cells to laminin Open
Could the phenomenon of catch bonding—force-strengthened cellular adhesion—play a role in sickle cell disease, where abnormal red blood cell (RBC) adhesion obstructs blood flow? Here we investigate the dynamics of sickle RBCs adhering to a…
View article: OcclusionChip: A functional microcapillary occlusion assay complementary to ektacytometry for detection of small-fraction red blood cells with abnormal deformability
OcclusionChip: A functional microcapillary occlusion assay complementary to ektacytometry for detection of small-fraction red blood cells with abnormal deformability Open
Red blood cell (RBC) deformability is a valuable hemorheological biomarker that can be used to assess the clinical status and response to therapy of individuals with sickle cell disease (SCD). RBC deformability has been measured by ektacyt…
View article: <scp>Antithrombin‐III</scp> mitigates thrombin‐mediated endothelial cell contraction and sickle red blood cell adhesion in microscale flow
<span>Antithrombin‐III</span> mitigates thrombin‐mediated endothelial cell contraction and sickle red blood cell adhesion in microscale flow Open
Summary Individuals with sickle cell disease (SCD) have persistently elevated thrombin generation that results in a state of systemic hypercoagulability. Antithrombin‐III (ATIII), an endogenous serine protease inhibitor, inhibits several e…
View article: PUM1 mediates the posttranscriptional regulation of human fetal hemoglobin
PUM1 mediates the posttranscriptional regulation of human fetal hemoglobin Open
The fetal-to-adult hemoglobin switching at about the time of birth involves a shift in expression from γ-globin to β-globin in erythroid cells. Effective re-expression of fetal γ-globin can ameliorate sickle cell anemia and β-thalassemia. …
View article: Sickle red blood cell derived extracellular vesicles activate endothelial cells and enhance sickle red cell adhesion mediated by von Willebrand factor
Sickle red blood cell derived extracellular vesicles activate endothelial cells and enhance sickle red cell adhesion mediated by von Willebrand factor Open
Endothelial activation and sickle red blood cell (RBC) adhesion are central to the pathogenesis of sickle cell disease (SCD). Quantitatively, RBC-derived extracellular vesicles, REVs, are more abundant from SS RBCs compared with healthy RB…
View article: Anticipatory Burden in Adult-Child Caregivers: A Concept Analysis
Anticipatory Burden in Adult-Child Caregivers: A Concept Analysis Open
This study aims to analyze the concept of anticipatory burden in adult-child caregivers. A systematic literature review was performed using four databases, Pubmed, CINAHL, PsycINFO and Medline, with the keywords of “anticipatory burden” an…
View article: Multispectral Imaging for MicroChip Electrophoresis Enables Point-of-Care Newborn Hemoglobin Variant Screening
Multispectral Imaging for MicroChip Electrophoresis Enables Point-of-Care Newborn Hemoglobin Variant Screening Open
Hemoglobin (Hb) disorders affect nearly 7% of the world’s population. Globally, around 400,000 babies are born annually with sickle cell disease (SCD), primarily in sub-Saharan Africa where morbidity and mortality rates are high. Although …
View article: Blood Flow Velocimetry in a Microchannel During Coagulation Using Particle Image Velocimetry and Wavelet-Based Optical Flow Velocimetry
Blood Flow Velocimetry in a Microchannel During Coagulation Using Particle Image Velocimetry and Wavelet-Based Optical Flow Velocimetry Open
This article describes novel measurements of the velocity of whole blood flow in a microchannel during coagulation. The blood is imaged volumetrically using a simple optical setup involving a white light source and a microscope camera. The…
View article: Cover Image
Cover Image Open
The cover image is based on the Original Article Standardized microfl uidic assessment of red blood cell mediated microcapillary occlusion: association with clinical phenotype and hydroxyurea responsiveness in sickle cell disease by Umut G…
View article: Emerging point-of-care technologies for anemia detection
Emerging point-of-care technologies for anemia detection Open
In this critical review, emerging non-invasive and minimally invasive point-of-care anemia detection technologies are discussed, highlighting future directions and the need for multiplexed approaches and integrated disease etiology identif…
View article: Microfluidic electrical impedance assessment of red blood cell-mediated microvascular occlusion
Microfluidic electrical impedance assessment of red blood cell-mediated microvascular occlusion Open
Microfluidic Impedance Red Cell Assay (MIRCA) is integrated with capillary network-inspired micropillar arrays and electrical impedance sensing electrodes to enable standardized assessment of red blood cell-mediated microvascular occlusion.