Yuriko Sakaguchi
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View article: Translation dysregulation in cancer as a source for targetable antigens
Translation dysregulation in cancer as a source for targetable antigens Open
Aberrant peptides presented by major histocompatibility complex (MHC) molecules are targets for tumor eradication, as these peptides can be recognized as foreign by T cells. Protein synthesis in malignant cells is dysregulated, which may r…
View article: <scp>ICLAMP</scp> : a novel technique to explore adenosine deamination via inosine chemical labeling and affinity molecular purification
<span>ICLAMP</span> : a novel technique to explore adenosine deamination via inosine chemical labeling and affinity molecular purification Open
Recent developments in sequencing and bioinformatics have advanced our understanding of adenosine‐to‐inosine (A‐to‐I) RNA editing. Surprisingly, recent analyses have revealed the capability of adenosine deaminase acting on RNA (ADAR) to ed…
View article: Substrate specificity of <i>Mycobacterium tuberculosis</i> tRNA terminal nucleotidyltransferase toxin MenT3
Substrate specificity of <i>Mycobacterium tuberculosis</i> tRNA terminal nucleotidyltransferase toxin MenT3 Open
Mycobacterium tuberculosis transfer RNA (tRNA) terminal nucleotidyltransferase toxin, MenT3, incorporates nucleotides at the 3′-CCA end of tRNAs, blocking their aminoacylation and inhibiting protein synthesis. Here, we show that MenT3 most…
View article: An anticodon-sensing T-boxzyme generates the elongator nonproteinogenic aminoacyl-tRNA <i>in situ</i> of a custom-made translation system for incorporation
An anticodon-sensing T-boxzyme generates the elongator nonproteinogenic aminoacyl-tRNA <i>in situ</i> of a custom-made translation system for incorporation Open
In the hypothetical RNA world, ribozymes could have acted as modern aminoacyl-tRNA synthetases (ARSs) to charge tRNAs, thus giving rise to the peptide synthesis along with the evolution of a primitive translation apparatus. We previously r…
View article: Reversible RNA phosphorylation stabilizes tRNA for cellular thermotolerance
Reversible RNA phosphorylation stabilizes tRNA for cellular thermotolerance Open
Post-transcriptional modifications have critical roles in tRNA stability and function 1–4 . In thermophiles, tRNAs are heavily modified to maintain their thermal stability under extreme growth temperatures 5,6 . Here we identified 2′-phosp…
View article: Mechanistic insights into tRNA cleavage by a contact-dependent growth inhibitor protein and translation factors
Mechanistic insights into tRNA cleavage by a contact-dependent growth inhibitor protein and translation factors Open
Contact-dependent growth inhibition is a mechanism of interbacterial competition mediated by delivery of the C-terminal toxin domain of CdiA protein (CdiA–CT) into neighboring bacteria. The CdiA–CT of enterohemorrhagic Escherichia coli EC8…
View article: Molecular basis of glycyl-tRNAGly acetylation by TacT from Salmonella Typhimurium
Molecular basis of glycyl-tRNAGly acetylation by TacT from Salmonella Typhimurium Open
Bacterial toxin-antitoxin modules contribute to the stress adaptation, persistence, and dormancy of bacteria for survival under environmental stresses and are involved in bacterial pathogenesis. In Salmonella Typhimurium, the Gcn5-related …
View article: The Effect of tRNA[Ser]Sec Isopentenylation on Selenoprotein Expression
The Effect of tRNA[Ser]Sec Isopentenylation on Selenoprotein Expression Open
Transfer RNA[Ser]Sec carries multiple post-transcriptional modifications. The A37G mutation in tRNA[Ser]Sec abrogates isopentenylation of base 37 and has a profound effect on selenoprotein expression in mice. Patients with a homozygous pat…
View article: Dynamic changes in tRNA modifications and abundance during T cell activation
Dynamic changes in tRNA modifications and abundance during T cell activation Open
The tRNA pool determines the efficiency, throughput, and accuracy of translation. Previous studies have identified dynamic changes in the tRNA (transfer RNA) supply and mRNA (messenger RNA) demand during cancerous proliferation. Yet dynami…
View article: Complete chemical structures of human mitochondrial tRNAs
Complete chemical structures of human mitochondrial tRNAs Open
Mitochondria generate most cellular energy via oxidative phosphorylation. Twenty-two species of mitochondrial (mt-)tRNAs encoded in mtDNA translate essential subunits of the respiratory chain complexes. mt-tRNAs contain post-transcriptiona…
View article: Epigenetic loss of the transfer RNA-modifying enzyme TYW2 induces ribosome frameshifts in colon cancer
Epigenetic loss of the transfer RNA-modifying enzyme TYW2 induces ribosome frameshifts in colon cancer Open
Significance Defects in transfer RNA (tRNA) modifications occur in human pathologies such as cancer; however, how these alterations contribute to the disease is poorly understood. One example is the tumor-specific hypomodification of posit…
View article: Substrate specificities of Escherichia coli ItaT that acetylates aminoacyl-tRNAs
Substrate specificities of Escherichia coli ItaT that acetylates aminoacyl-tRNAs Open
Escherichia coli ItaT toxin reportedly acetylates the α-amino group of the aminoacyl-moiety of Ile-tRNAIle specifically, using acetyl-CoA as an acetyl donor, thereby inhibiting protein synthesis. The mechanism of the substrate specificity …
View article: Dynamic changes in tRNA modifications and abundance during T-cell activation
Dynamic changes in tRNA modifications and abundance during T-cell activation Open
The tRNA pool determines the efficiency, throughput, and accuracy of translation. Previous studies have identified dynamic changes in the tRNA supply and mRNA demand during cancerous proliferation. Yet, dynamic changes may occur also durin…
View article: Metabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease
Metabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease Open
Modified uridine containing taurine, 5-taurinomethyluridine (τm(5)U), is found at the anticodon first position of mitochondrial (mt-)transfer RNAs (tRNAs). Previously, we reported that τm(5)U is absent in mt-tRNAs with pathogenic mutations…
View article: Biochemical and structural characterization of oxygen-sensitive 2-thiouridine synthesis catalyzed by an iron-sulfur protein TtuA
Biochemical and structural characterization of oxygen-sensitive 2-thiouridine synthesis catalyzed by an iron-sulfur protein TtuA Open
Significance One of the posttranscriptional modifications of tRNA, 2-thiouridine (s 2 U), enhances thermostability. Although extensive studies have been conducted to understand the mechanism behind this modification, many ill-defined point…
View article: Human BCDIN3D monomethylates cytoplasmic histidine transfer RNA
Human BCDIN3D monomethylates cytoplasmic histidine transfer RNA Open
Human RNA methyltransferase BCDIN3D is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. Here, we show that cytoplasmic tRNAHis is the primary target of BCDIN3D in human …
View article: Identification of 2-methylthio cyclic N6-threonylcarbamoyladenosine (ms2ct6A) as a novel RNA modification at position 37 of tRNAs
Identification of 2-methylthio cyclic N6-threonylcarbamoyladenosine (ms2ct6A) as a novel RNA modification at position 37 of tRNAs Open
Transfer RNA modifications play pivotal roles in protein synthesis. N6-threonylcarbamoyladenosine (t6A) and its derivatives are modifications found at position 37, 3΄-adjacent to the anticodon, in tRNAs responsible for ANN codons. These mo…