Yusuf Dölen
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View article: Galectin-9 regulates dendritic cell polarity and uropod contraction by modulating RhoA activity
Galectin-9 regulates dendritic cell polarity and uropod contraction by modulating RhoA activity Open
Adaptive immunity relies on dendritic cell (DC) migration to transport antigens from tissues to lymph nodes. Galectins, a family of β-galactoside–binding proteins, control cell membrane organization, exerting crucial roles in multiple phys…
View article: Immunofilaments Are Well Tolerated after Local or Systemic Administration in Mice
Immunofilaments Are Well Tolerated after Local or Systemic Administration in Mice Open
The invention of nanosized biomaterials has paved the way for novel therapeutics that can manipulate cells on a nanoscale. Nanosized immunofilaments (IFs) are synthetic filamentous polymers consisting out of polyisocyanopeptides, which hav…
View article: pH and ROS Responsiveness of Polymersome Nanovaccines for Antigen and Adjuvant Codelivery: An In Vitro and In Vivo Comparison
pH and ROS Responsiveness of Polymersome Nanovaccines for Antigen and Adjuvant Codelivery: An In Vitro and In Vivo Comparison Open
The antitumor immunity can be enhanced through the synchronized codelivery of antigens and immunostimulatory adjuvants to antigen-presenting cells, particularly dendritic cells (DCs), using nanovaccines (NVs). To study the influence of int…
View article: Galectin-9 regulates dendritic cell polarity and uropod contraction by modulating RhoA activity
Galectin-9 regulates dendritic cell polarity and uropod contraction by modulating RhoA activity Open
Adaptive immunity relies on dendritic cell (DC) migration to transport antigens from tissues to lymph nodes. Galectins, a family of β-galactoside-binding proteins, control cell membrane organisation, exerting crucial roles in multiple phys…
View article: Direct In Vivo Activation of T Cells with Nanosized Immunofilaments Inhibits Tumor Growth and Metastasis
Direct In Vivo Activation of T Cells with Nanosized Immunofilaments Inhibits Tumor Growth and Metastasis Open
Adoptive T cell therapy has successfully been implemented for the treatment of cancer. Nevertheless, ex vivo expansion of T cells by artificial antigen-presenting cells (aAPCs) remains cumbersome and can compromise T cell functionality, th…
View article: Immunofilaments Provide a Nanoscale Platform for In Vivo T Cell Expansion and Cancer Immunotherapy
Immunofilaments Provide a Nanoscale Platform for In Vivo T Cell Expansion and Cancer Immunotherapy Open
Adoptive T cell therapy has successfully been implemented for the treatment of cancer. Nevertheless, the precarious ex vivo expansion of T cells by artificial antigen-presenting cells (aAPCs) remains cumbersome and can compromise T cell fu…
View article: Efficient targeting of NY-ESO-1 tumor antigen to human cDC1s by lymphotactin results in cross-presentation and antigen-specific T cell expansion
Efficient targeting of NY-ESO-1 tumor antigen to human cDC1s by lymphotactin results in cross-presentation and antigen-specific T cell expansion Open
Background Type 1 conventional dendritic cells (cDC1s) are characterized by their ability to induce potent CD8 + T cell responses. In efforts to generate novel vaccination strategies, notably against cancer, human cDC1s emerge as an ideal …
View article: Efficient targeting of NY-ESO-1 tumor antigen to human cDC1s by lymphotactin results in cross-presentation and antigen-specific T cell expansion
Efficient targeting of NY-ESO-1 tumor antigen to human cDC1s by lymphotactin results in cross-presentation and antigen-specific T cell expansion Open
Type 1 conventional dendritic cells (cDC1s) are characterized by their ability to induce potent CD8 + T cell responses. In efforts to generate novel vaccination strategies, notably against cancer, human cDC1s emerge as an ideal target to d…
View article: Enhanced Antitumor Efficacy through an “AND gate” Reactive Oxygen‐Species‐Dependent pH‐Responsive Nanomedicine Approach
Enhanced Antitumor Efficacy through an “AND gate” Reactive Oxygen‐Species‐Dependent pH‐Responsive Nanomedicine Approach Open
Anticancer drug delivery strategies are designed to take advantage of the differential chemical environment in solid tumors independently, or to high levels of reactive oxygen species (ROS) or to low pH, compared to healthy tissue. Here, t…
View article: PLGA Nanoparticles Co-encapsulating NY-ESO-1 Peptides and IMM60 Induce Robust CD8 and CD4 T Cell and B Cell Responses
PLGA Nanoparticles Co-encapsulating NY-ESO-1 Peptides and IMM60 Induce Robust CD8 and CD4 T Cell and B Cell Responses Open
Tumor-specific neoantigens can be highly immunogenic, but their identification for each patient and the production of personalized cancer vaccines can be time-consuming and prohibitively expensive. In contrast, tumor-associated antigens ar…
View article: Nanovaccine administration route is critical to obtain pertinent iNKt cell help for robust anti-tumor T and B cell responses
Nanovaccine administration route is critical to obtain pertinent iNKt cell help for robust anti-tumor T and B cell responses Open
Nanovaccines, co-delivering antigen and invariant natural killer T (iNKT) cell agonists, proved to be very effective in inducing anti-tumor T cell responses due to their exceptional helper function. However, it is known that iNKT cells are…
View article: Cross-talk between iNKT cells and CD8 T cells in the spleen requires the IL-4/CCL17 axis for the generation of short-lived effector cells
Cross-talk between iNKT cells and CD8 T cells in the spleen requires the IL-4/CCL17 axis for the generation of short-lived effector cells Open
Mounting an effective immune response relies critically on the coordinated interactions between adaptive and innate compartments. How and where immune cells from these different compartments interact is still poorly understood. Here, we de…
View article: Microfluidics-Assisted Size Tuning and Biological Evaluation of PLGA Particles
Microfluidics-Assisted Size Tuning and Biological Evaluation of PLGA Particles Open
Polymeric particles made up of biodegradable and biocompatible polymers such as poly(lactic-co-glycolic acid) (PLGA) are promising tools for several biomedical applications including drug delivery. Particular emphasis is placed on the size…
View article: Microfluidics-Assisted Size Tuning and Biological Evaluation of PLGA Particles
Microfluidics-Assisted Size Tuning and Biological Evaluation of PLGA Particles Open
Polymeric particles made up of biodegradable and biocompatible polymers such as poly(lactic-co-glycolic acid) (PLGA) are promising tools for several biomedical applications including drug delivery. Particular emphasis is placed on the size…
View article: Functional diversification of hybridoma-produced antibodies by CRISPR/HDR genomic engineering
Functional diversification of hybridoma-produced antibodies by CRISPR/HDR genomic engineering Open
We develop a universal platform for CRISPR/HDR-engineering of hybridomas to obtain functionally diverse antibody panels.
View article: Multicore Liquid Perfluorocarbon‐Loaded Multimodal Nanoparticles for Stable Ultrasound and <sup>19</sup>F MRI Applied to In Vivo Cell Tracking
Multicore Liquid Perfluorocarbon‐Loaded Multimodal Nanoparticles for Stable Ultrasound and <sup>19</sup>F MRI Applied to In Vivo Cell Tracking Open
Ultrasound is the most commonly used clinical imaging modality. However, in applications requiring cell‐labeling, the large size and short active lifetime of ultrasound contrast agents limit their longitudinal use. Here, 100 nm radius, cli…
View article: Functional diversification of hybridoma produced antibodies by CRISPR/HDR genomic engineering
Functional diversification of hybridoma produced antibodies by CRISPR/HDR genomic engineering Open
Hybridoma technology is instrumental for the development of novel antibody therapeutics and diagnostics. Recent preclinical and clinical studies highlight the importance of antibody isotype for therapeutic efficacy. However, since the sequ…
View article: Injectable Biomimetic Hydrogels as Tools for Efficient T Cell Expansion and Delivery
Injectable Biomimetic Hydrogels as Tools for Efficient T Cell Expansion and Delivery Open
Biomaterial-based scaffolds are promising tools for controlled immunomodulation. They can be applied as three dimensional (3D) culture systems in vitro, whereas in vivo they may be used to dictate cellular localization and exert spatiotemp…
View article: Endolysosomal‐Escape Nanovaccines through Adjuvant‐Induced Tumor Antigen Assembly for Enhanced Effector CD8<sup>+</sup> T Cell Activation
Endolysosomal‐Escape Nanovaccines through Adjuvant‐Induced Tumor Antigen Assembly for Enhanced Effector CD8<sup>+</sup> T Cell Activation Open
The activation of tumor‐specific effector immune cells is key for successful immunotherapy and vaccination is a powerful strategy to induce such adaptive immune responses. However, the generation of effective anticancer vaccines is challen…