Yutaka Sugihara
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View article: Distant metastases of melanoma exhibit varying extent of intrapatient proteogenomic heterogeneity
Distant metastases of melanoma exhibit varying extent of intrapatient proteogenomic heterogeneity Open
Background Metastatic melanoma is a highly aggressive disease with poor survival rates despite recent therapeutic advancements with immunotherapy. The proteomic landscape of advanced melanoma remains poorly understood, especially regarding…
View article: Mitochondrial proteome landscape unveils key insights into melanoma severity and treatment strategies
Mitochondrial proteome landscape unveils key insights into melanoma severity and treatment strategies Open
Background Melanoma, the deadliest form of skin cancer, exhibits resistance to conventional therapies, particularly in advanced and metastatic stages. Mitochondrial pathways, including oxidative phosphorylation and mitochondrial translatio…
View article: Proteogenomic Profiling of Treatment-Naïve Metastatic Malignant Melanoma
Proteogenomic Profiling of Treatment-Naïve Metastatic Malignant Melanoma Open
Background: Melanoma is a highly heterogeneous disease, and a deeper molecular classification is essential for improving patient stratification and treatment approaches. Here, we describe the histopathology-driven proteogenomic landscape o…
View article: Histopathology-assisted proteogenomics provides foundations for stratification of melanoma metastases
Histopathology-assisted proteogenomics provides foundations for stratification of melanoma metastases Open
Here we describe the histopathology-driven proteogenomic landscape of 142 treatment-naïve metastatic melanoma samples. We identified five proteomic subtypes that integrate the immune and stroma microenvironment components, and associate wi…
View article: Proteogenomic Characterization Reveals Therapeutic Opportunities Related to Mitochondrial Function in Melanoma
Proteogenomic Characterization Reveals Therapeutic Opportunities Related to Mitochondrial Function in Melanoma Open
Summary The dynamics of more than 1900 mitochondrial proteins was explored through quantitative proteomics in 151 melanoma-related tissue samples of both surgical and autopsy origin. Dysregulation of mitochondrial pathways in primary tumor…
View article: An Observational Study on the Molecular Profiling of Primary Melanomas Reveals a Progression Dependence on Mitochondrial Activation
An Observational Study on the Molecular Profiling of Primary Melanomas Reveals a Progression Dependence on Mitochondrial Activation Open
Melanoma in advanced stages is one of the most aggressive tumors and the deadliest of skin cancers. To date, the histopathological staging focuses on tumor thickness, and clinical staging is a major estimate of the clinical behavior of pri…
View article: The human melanoma proteome atlas—Defining the molecular pathology
The human melanoma proteome atlas—Defining the molecular pathology Open
The MM500 study is an initiative to map the protein levels in malignant melanoma tumor samples, focused on in‐depth histopathology coupled to proteome characterization. The protein levels and localization were determined for a broad spectr…
View article: The Human Melanoma Proteome Atlas—Complementing the melanoma transcriptome
The Human Melanoma Proteome Atlas—Complementing the melanoma transcriptome Open
The MM500 meta‐study aims to establish a knowledge basis of the tumor proteome to serve as a complement to genome and transcriptome studies. Somatic mutations and their effect on the transcriptome have been extensively characterized in mel…
View article: Matrix‐assisted laser desorption ionization ‐ mass spectrometry imaging of erlotinib reveals a limited tumor tissue distribution in a non‐small‐cell lung cancer mouse xenograft model
Matrix‐assisted laser desorption ionization ‐ mass spectrometry imaging of erlotinib reveals a limited tumor tissue distribution in a non‐small‐cell lung cancer mouse xenograft model Open
Erlotinib has been used to treat patients with EGFR-mutated non-small-cell lung cancer (NSCLC) for almost two decades; however, acquired resistance sooner or later develops against its blockade, thus, low efficacy is inevitable in some pat…
View article: Proteogenomics Reveals how Metastatic Melanoma Modulates the Immune System to Allow Immune Evasion
Proteogenomics Reveals how Metastatic Melanoma Modulates the Immune System to Allow Immune Evasion Open
Summary Malignant melanoma (MM) develops from the melanocytes and in its advanced stage is the most aggressive type of skin cancer. Here we report a comprehensive analysis on a prospective cohort study, including non-tumor, primary and met…
View article: Identification and Validation of VEGFR2 Kinase as a Target of Voacangine by a Systematic Combination of DARTS and MSI
Identification and Validation of VEGFR2 Kinase as a Target of Voacangine by a Systematic Combination of DARTS and MSI Open
Although natural products are an important source of drugs and drug leads, identification and validation of their target proteins have proven difficult. Here, we report the development of a systematic strategy for target identification and…
View article: Identification and Validation of VEGFR2 Kinase as a Target of Voacangine by a Systematic Combination of DARTS and MSI
Identification and Validation of VEGFR2 Kinase as a Target of Voacangine by a Systematic Combination of DARTS and MSI Open
Although natural products are an important source of drugs and drug leads, identification and validation of their target proteins have proven difficult. Here, we report the development of a systematic strategy for target identification and…
View article: Amyloid-specific extraction using organic solvents
Amyloid-specific extraction using organic solvents Open
View article: The Hidden Story of Heterogeneous B-raf V600E Mutation Quantitative Protein Expression in Metastatic Melanoma—Association with Clinical Outcome and Tumor Phenotypes
The Hidden Story of Heterogeneous B-raf V600E Mutation Quantitative Protein Expression in Metastatic Melanoma—Association with Clinical Outcome and Tumor Phenotypes Open
In comparison to other human cancer types, malignant melanoma exhibits the greatest amount of heterogeneity. After DNA-based detection of the BRAF V600E mutation in melanoma patients, targeted inhibitor treatment is the current recommendat…
View article: The Hidden Story on Heterogeneous B-raf V600E Mutation Quantitative Protein Expression in Metastatic Melanoma – Association with Clinical Outcome and Tumor Phenotypes
The Hidden Story on Heterogeneous B-raf V600E Mutation Quantitative Protein Expression in Metastatic Melanoma – Association with Clinical Outcome and Tumor Phenotypes Open
In comparison to other human cancer types, malignant melanoma exhibits the greatest amount of heterogeneity. After DNA-based detection of the BRAF V600E mutation in melanoma patients, targeted inhibitor treatment is the current recommendat…
View article: Novel functional proteins coded by the human genome discovered in metastases of melanoma patients
Novel functional proteins coded by the human genome discovered in metastases of melanoma patients Open
View article: Improved survival prognostication of node-positive malignant melanoma patients utilizing shotgun proteomics guided by histopathological characterization and genomic data
Improved survival prognostication of node-positive malignant melanoma patients utilizing shotgun proteomics guided by histopathological characterization and genomic data Open
View article: Clinical protein science in translational medicine targeting malignant melanoma
Clinical protein science in translational medicine targeting malignant melanoma Open
View article: Challenging the heterogeneity of disease presentation in malignant melanoma—impact on patient treatment
Challenging the heterogeneity of disease presentation in malignant melanoma—impact on patient treatment Open
View article: Biobank integration of large‐scale clinical and histopathology melanoma studies within the European Cancer Moonshot Lund Center
Biobank integration of large‐scale clinical and histopathology melanoma studies within the European Cancer Moonshot Lund Center Open
We present the Cancer Moonshot clinical project located at the European center in Lund. Here, tissue and blood samples have been collected and stored in a large‐scale biobank. Multiple clinical centers around the world are participating an…
View article: Endogenous expression mapping of malignant melanoma by mass spectrometry imaging
Endogenous expression mapping of malignant melanoma by mass spectrometry imaging Open
Background Currently, only a limited number of molecular biomarkers for malignant melanoma exist. This is the case for both diagnosing the disease, staging, and efficiently measuring the response to therapy by tracing the progression of di…
View article: Correlation of histopathologic characteristics to protein expression and function in malignant melanoma
Correlation of histopathologic characteristics to protein expression and function in malignant melanoma Open
In this feasibility study, promising results show the feasibility of relating proteomics to histopathology and clinical outcome, and insight thus can be gained into the molecular processes driving the disease. The combined analysis of hist…
View article: Limited Tumor Tissue Drug Penetration Contributes to Primary Resistance against Angiogenesis Inhibitors
Limited Tumor Tissue Drug Penetration Contributes to Primary Resistance against Angiogenesis Inhibitors Open
Resistance mechanisms against antiangiogenic drugs are unclear. Here, we correlated the antitumor and antivascular properties of five different antiangiogenic receptor tyrosine kinase inhibitors (RTKIs) (motesanib, pazopanib, sorafenib, su…
View article: Workflow for large-scale analysis of melanoma tissue samples
Workflow for large-scale analysis of melanoma tissue samples Open
The aim of the present study was to create an optimal workflow for analysing a large cohort of malignant melanoma tissue samples. Samples were lysed with urea and enzymatically digested with trypsin or trypsin/Lys C. Buffer exchange or dil…
View article: A Protein Deep Sequencing Evaluation of Metastatic Melanoma Tissues
A Protein Deep Sequencing Evaluation of Metastatic Melanoma Tissues Open
Malignant melanoma has the highest increase of incidence of malignancies in the western world. In early stages, front line therapy is surgical excision of the primary tumor. Metastatic disease has very limited possibilities for cure. Recen…