Yuuta Imoto
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View article: Beyond Clathrin: Decoding the Mechanism of Ultrafast Endocytosis
Beyond Clathrin: Decoding the Mechanism of Ultrafast Endocytosis Open
Endocytosis in non-neuronal cells requires gradual recruitment of proteins to endocytic sites for inducing membrane curvature and forming contractile scaffolds around the neck of endocytic pits. This recruitment process is thought to be ra…
View article: Ultrastructural membrane dynamics of mouse and human cortical synapses
Ultrastructural membrane dynamics of mouse and human cortical synapses Open
Live human brain tissues provide unique opportunities for understanding synaptic transmission. Investigations have been limited to anatomy, electrophysiology, and protein localization, while crucial parameters such as synaptic vesicle dyna…
View article: Dynamin 1xA interacts with Endophilin A1 via its spliced long C-terminus for ultrafast endocytosis
Dynamin 1xA interacts with Endophilin A1 via its spliced long C-terminus for ultrafast endocytosis Open
Summary Dynamin 1 (Dyn1) has two major splice variants, xA and xB, with unique C-terminal extensions of 20 and 7 amino acids, respectively. Of these, only Dyn1xA is enriched at endocytic zones and accelerates vesicle fission during ultrafa…
View article: Dynamin is primed at endocytic sites for ultrafast endocytosis
Dynamin is primed at endocytic sites for ultrafast endocytosis Open
Dynamin mediates fission of vesicles from the plasma membrane during endocytosis. Typically, dynamin is recruited from the cytosol to endocytic sites, requiring seconds to tens of seconds. However, ultrafast endocytosis in neurons internal…
View article: Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis
Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis Open
Compensatory endocytosis keeps the surface area of secretory cells constant following exocytosis. At chemical synapses, clathrin-independent ultrafast endocytosis maintains such homeostasis. This endocytic pathway is temporally and spatial…
View article: Dynamin forms liquid-like condensates at synapses to support ultrafast endocytosis
Dynamin forms liquid-like condensates at synapses to support ultrafast endocytosis Open
Summary Endocytosis at synapses is accelerated by the pre-accumulation of Dynamin 1xA at the endocytic zone by Syndapin 1. However, it is unclear how these proteins support the ultrafast kinetics of endocytosis. Here we report that these p…
View article: Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradation
Axonal transport of Hrs is activity dependent and facilitates synaptic vesicle protein degradation Open
Turnover of synaptic vesicle (SV) proteins is vital for the maintenance of healthy and functional synapses. SV protein turnover is driven by neuronal activity in an endosomal sorting complex required for transport (ESCRT)-dependent manner.…
View article: Smooth Loop-Like Mitochondrial Nucleus in the Primitive Red Alga <i>Cyanidioschyzon merolae</i> Revealed by Drying Treatment
Smooth Loop-Like Mitochondrial Nucleus in the Primitive Red Alga <i>Cyanidioschyzon merolae</i> Revealed by Drying Treatment Open
It is thought that mitochondria were generated by the symbiosis of autonomous α-proteobacteria and a eukaryote-like organism derived from an archaeon of the species Sulfolobus. Soon after the symbiosis, most of the genome of the α-proteoba…
View article: Dynamin is primed at endocytic sites for ultrafast endocytosis
Dynamin is primed at endocytic sites for ultrafast endocytosis Open
Summary Dynamin mediates fission of vesicles from the plasma membrane during endocytosis. Typically, dynamin is recruited from the cytosol to endocytic sites, requiring seconds to tens of seconds. However, ultrafast endocytosis in neurons …
View article: Mammalian Homologue NME3 of DYNAMO1 Regulates Peroxisome Division
Mammalian Homologue NME3 of DYNAMO1 Regulates Peroxisome Division Open
Peroxisomes proliferate by sequential processes comprising elongation, constriction, and scission of peroxisomal membrane. It is known that the constriction step is mediated by a GTPase named dynamin-like protein 1 (DLP1) upon efficient lo…
View article: Molecular Basis of Mitochondrial and Peroxisomal Division Machineries
Molecular Basis of Mitochondrial and Peroxisomal Division Machineries Open
Mitochondria and peroxisomes are ubiquitous subcellular organelles that are highly dynamic and possess a high degree of plasticity. These organelles proliferate through division of pre-existing organelles. Studies on yeast, mammalian cells…
View article: Mitotic Karyotype of the Primitive Red Alga <i>Cyanidioschyzon merolae</i> 10D
Mitotic Karyotype of the Primitive Red Alga <i>Cyanidioschyzon merolae</i> 10D Open
It is important to understand how a single circular chromosome in the prokaryotic nucleus evolved into multiple linear chromosomes in the eukaryotic nucleus. In most eukaryotic cells that have ＞∼15 Mbp of genomic DNA, chromosomes remain co…
View article: Recent insights into peroxisome biogenesis and associated diseases
Recent insights into peroxisome biogenesis and associated diseases Open
Peroxisomes are single-membrane organelles present in eukaryotes. The functional importance of peroxisomes in humans is represented by peroxisome-deficient peroxisome biogenesis disorders (PBDs), including Zellweger syndrome. Defects in th…
View article: Axonal transport of Hrs is activity-dependent and rate limiting for synaptic vesicle protein degradation
Axonal transport of Hrs is activity-dependent and rate limiting for synaptic vesicle protein degradation Open
Turnover of synaptic vesicle (SV) proteins is vital for the maintenance of healthy and functional synapses. SV protein turnover is driven by neuronal activity in an ESCRT (endosomal sorting complex required for transport)-dependent manner.…
View article: ESCRT Machinery Mediates Cytokinetic Abscission in the Unicellular Red Alga Cyanidioschyzon merolae
ESCRT Machinery Mediates Cytokinetic Abscission in the Unicellular Red Alga Cyanidioschyzon merolae Open
In many eukaryotes, cytokinesis proceeds in two successive steps: first, ingression of the cleavage furrow and second, abscission of the intercellular bridge. In animal cells, the actomyosin contractile ring is involved in the first step, …
View article: Molecular basis of local energy generation during mitochondrial and peroxisomal division
Molecular basis of local energy generation during mitochondrial and peroxisomal division Open
GTPase dynamin-related protein (Dnm1)-mediated membrane fission is an important membrane remodeling event supporting the proliferation and housekeeping function of semiautonomous organelles such as the mitochondrion and peroxisome. Dnm1 is…
View article: Dynamics of the nucleoside diphosphate kinase protein DYNAMO2 correlates with the changes in the global GTP level during the cell cycle of <i>Cyanidioschyzon merolae</i>
Dynamics of the nucleoside diphosphate kinase protein DYNAMO2 correlates with the changes in the global GTP level during the cell cycle of <i>Cyanidioschyzon merolae</i> Open
GTP is an essential source of energy that supports a large array of cellular mechanochemical structures ranging from protein synthesis machinery to cytoskeletal apparatus for maintaining the cell cycle. However, GTP regulation during the c…
View article: Glycosyltransferase MDR1 assembles a dividing ring for mitochondrial proliferation comprising polyglucan nanofilaments
Glycosyltransferase MDR1 assembles a dividing ring for mitochondrial proliferation comprising polyglucan nanofilaments Open
Significance The mitochondrion-dividing (MD) ring mediates binary division of mitochondria. However, the molecular identity of the MD ring is currently unknown. We show that the glycosyltransferase MITOCHONDRION-DIVIDING RING1 (MDR1) regul…
View article: Defining the dynamin-based ring organizing center on the peroxisome-dividing machinery isolated from <i>Cyanidioschyzon merolae</i>
Defining the dynamin-based ring organizing center on the peroxisome-dividing machinery isolated from <i>Cyanidioschyzon merolae</i> Open
Organelle division is executed through contraction of a ring-shaped supramolecular dividing machinery. A core component of the machinery is the dynamin-based ring conserved during the division of mitochondrion, plastid and peroxisome. Here…
View article: Genome Size of the Ultrasmall Unicellular Freshwater Green Alga, <i>Medakamo hakoo</i> 311, as Determined by Staining with 4′,6-Diamidino-2-phenylindole after Microwave Oven Treatments: II. Comparison with <i>Cyanidioschyzon merolae</i>, <i>Saccharomyces cerevisiae</i> (<i>n</i>, 2<i>n</i>), and <i>Chlorella variabilis</i>
Genome Size of the Ultrasmall Unicellular Freshwater Green Alga, <i>Medakamo hakoo</i> 311, as Determined by Staining with 4′,6-Diamidino-2-phenylindole after Microwave Oven Treatments: II. Comparison with <i>Cyanidioschyzon merolae</i>, <i>Saccharomyces cerevisiae</i> (<i>n</i>, 2<i>n</i>), and <i>Chlorella variabilis</i> Open
Eukaryotic cells contain three types of nuclei: the cell nucleus, the mitochondrial nuclei (nucleoids), and plastid nuclei (nucleoids). Here, we report that the chlorophyte strain Medakamo hakoo 311 has the smallest cell nucleus genome amo…
View article: Cytological Evidence of Cell-Nuclear Genome Size of a New Ultra-Small Unicellular Freshwater Green Alga, “<i>Medakamo hakoo</i>” strain M-hakoo 311 I. Comparison with <i>Cyanidioschyzon merolae</i> and <i>Ostreococcus tauri</i>
Cytological Evidence of Cell-Nuclear Genome Size of a New Ultra-Small Unicellular Freshwater Green Alga, “<i>Medakamo hakoo</i>” strain M-hakoo 311 I. Comparison with <i>Cyanidioschyzon merolae</i> and <i>Ostreococcus tauri</i> Open
Ultra-small unicellular algae provide information on the basic cellular mechanisms and essential genes that support the lives of photosynthetic eukaryotes, including higher plants. We have discovered the smallest free-living photosynthetic…