Zelin Shan
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View article: Nucleosome Engagement Regulates RORγt Structure and Dynamics
Nucleosome Engagement Regulates RORγt Structure and Dynamics Open
The retinoic acid-related orphan receptor gamma (RORγt) acts as the major transcriptional activator in Th17 cell development and function to mediate adaptive immune defenses against pathogenic infection. RORγt engages accessible DNA respon…
View article: Two-Metal Ion Mechanism of DNA Cleavage by Activated, Filamentous SgrAI
Two-Metal Ion Mechanism of DNA Cleavage by Activated, Filamentous SgrAI Open
Enzymes that form filamentous assemblies with modulated enzymatic activities have gained increasing attention in recent years. SgrAI is a sequence specific type II restriction endonuclease that forms polymeric filaments. SgrAI filamentatio…
View article: Oligomeric HIV-1 Integrase Structures Reveal Functional Plasticity for Intasome Assembly and RNA Binding
Oligomeric HIV-1 Integrase Structures Reveal Functional Plasticity for Intasome Assembly and RNA Binding Open
Integrase (IN) performs dual essential roles during HIV-1 replication. During ingress, IN functions within an oligomeric “intasome” assembly to catalyze viral DNA integration into host chromatin. During late stages of infection, tetrameric…
View article: Overcoming resolution attenuation during tilted cryo-EM data collection
Overcoming resolution attenuation during tilted cryo-EM data collection Open
Structural biology efforts using cryogenic electron microscopy are frequently stifled by specimens adopting “preferred orientations” on grids, leading to anisotropic map resolution and impeding structure determination. Tilting the specimen…
View article: Mechanisms of HIV-1 integrase resistance to dolutegravir and potent inhibition of drug-resistant variants
Mechanisms of HIV-1 integrase resistance to dolutegravir and potent inhibition of drug-resistant variants Open
HIV-1 infection depends on the integration of viral DNA into host chromatin. Integration is mediated by the viral enzyme integrase and is blocked by integrase strand transfer inhibitors (INSTIs), first-line antiretroviral therapeutics wide…
View article: Overcoming Resolution Attenuation During Tilted Cryo-EM Data Collection
Overcoming Resolution Attenuation During Tilted Cryo-EM Data Collection Open
Structural biology efforts using cryogenic electron microscopy are frequently stifled by specimens adopting “preferred orientations” on grids, leading to anisotropic map resolution and impeding structure determination. Tilting the specimen…
View article: A Unified Watson-Crick Geometry Drives Transcription of Six-Letter Expanded DNA Alphabets by Cellular RNA Polymerase
A Unified Watson-Crick Geometry Drives Transcription of Six-Letter Expanded DNA Alphabets by Cellular RNA Polymerase Open
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View article: Mechanisms of HIV-1 Integrase Resistance to Dolutegravir and Potent Inhibition of Drug Resistant Variants
Mechanisms of HIV-1 Integrase Resistance to Dolutegravir and Potent Inhibition of Drug Resistant Variants Open
HIV-1 infection depends on the integration of viral DNA into host chromatin. Integration is mediated by the viral enzyme integrase and is blocked by integrase strand transfer inhibitors (INSTIs), first-line antiretroviral therapeutics wide…
View article: Pretransition state and apo structures of the filament-forming enzyme SgrAI elucidate mechanisms of activation and substrate specificity
Pretransition state and apo structures of the filament-forming enzyme SgrAI elucidate mechanisms of activation and substrate specificity Open
Enzyme filamentation is a widespread phenomenon that mediates enzyme regulation and function. For the filament-forming sequence-specific DNA endonuclease SgrAI, the process of filamentation both accelerates its DNA cleavage activity and ex…
View article: Multivalent interactions essential for lentiviral integrase function
Multivalent interactions essential for lentiviral integrase function Open
A multimer of retroviral integrase (IN) synapses viral DNA ends within a stable intasome nucleoprotein complex for integration into a host cell genome. Reconstitution of the intasome from the maedi-visna virus (MVV), an ovine lentivirus, r…
View article: WW domain–mediated regulation and activation of E3 ubiquitin ligase Suppressor of Deltex
WW domain–mediated regulation and activation of E3 ubiquitin ligase Suppressor of Deltex Open
The Nedd4 family E3 ligases Itch and WWP1/2 play crucial roles in the regulation of cell cycle progression and apoptosis and are closely correlated with cancer development and metastasis. It has been recently shown that the ligase activiti…