Zengpeng Han
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View article: AAV11 mediates efficient retrograde trans-neuronal transduction of astrocytes
AAV11 mediates efficient retrograde trans-neuronal transduction of astrocytes Open
Understanding the mechanisms by which astrocytes regulate neural circuits is crucial for elucidating the mechanisms underlying cognitive and behavioral abnormalities in psychiatric disorders and for exploring potential therapeutic or inter…
View article: Investigating the relationships of structural and functional neural networks of primary visual cortex with engineered AAVs and chemogenetic-fMRI techniques
Investigating the relationships of structural and functional neural networks of primary visual cortex with engineered AAVs and chemogenetic-fMRI techniques Open
Rationale: Neural networks are crucial for brain function, but the relationship between functional and structural networks remains unclear, hindering disease understanding and treatment. This study employs AAVs, MRI reporter gene (A…
View article: Engineered AAV13 Variants with Enhanced Transduction and Confined Spread
Engineered AAV13 Variants with Enhanced Transduction and Confined Spread Open
Precise targeting of specific regions within the central nervous system (CNS) is crucial for both scientific research and gene therapy in the context of brain diseases. Adeno-associated virus 13 (AAV13) is known for its restricted diffusio…
View article: AAV11 enables efficient retrograde targeting of projection neurons and enhances astrocyte-directed transduction
AAV11 enables efficient retrograde targeting of projection neurons and enhances astrocyte-directed transduction Open
View article: CLEAR Strategy Inhibited HSV Proliferation Using Viral Vectors Delivered CRISPR-Cas9
CLEAR Strategy Inhibited HSV Proliferation Using Viral Vectors Delivered CRISPR-Cas9 Open
Herpes simplex virus type 1 (HSV-1) is a leading cause of encephalitis and infectious blindness. The commonly used clinical therapeutic drugs are nucleoside analogues such as acyclovir. However, current drugs for HSV cannot eliminate the l…
View article: Computer-Aided Directed Evolution Generates Novel AAV Variants with High Transduction Efficiency
Computer-Aided Directed Evolution Generates Novel AAV Variants with High Transduction Efficiency Open
Adeno-associated viruses (AAVs) have become safe and effective tools for therapeutic in vivo gene drug delivery. Among many AAV serotypes, AAV2 is the most well-characterized. Although many studies have been carried out on the engineering …
View article: Brain-wide N2cG compensation permits glycoprotein-deleted rabies virus to trace neural circuits across multiple synapses
Brain-wide N2cG compensation permits glycoprotein-deleted rabies virus to trace neural circuits across multiple synapses Open
Rabies-viruses-based retrograde tracers can spread across multiple synapses in a retrograde direction in the nervous system of rodents and primates, making them powerful tools for determining the structure and function of the complicated n…
View article: AAV13 Enables Precise Targeting of Local Neural Populations
AAV13 Enables Precise Targeting of Local Neural Populations Open
As powerful tools for local gene delivery, adeno-associated viruses (AAVs) are widely used for neural circuit studies and therapeutical purposes. However, most of them have the characteristics of large diffusion range and retrograde labeli…
View article: Brain-wide TVA compensation allows rabies virus to retrograde target cell-type-specific projection neurons
Brain-wide TVA compensation allows rabies virus to retrograde target cell-type-specific projection neurons Open
View article: Improved production and expanded application of CVS-N2c-ΔG virus for retrograde tracing
Improved production and expanded application of CVS-N2c-ΔG virus for retrograde tracing Open
Neurotropic virus tracers, particularly those with low toxicity and high efficient tracing, are powerful tools for structural and functional dissections of neural circuits. The retrograde trans-mono-synaptic technology based on rabies viru…
View article: AAV11 permits efficient retrograde targeting of projection neurons
AAV11 permits efficient retrograde targeting of projection neurons Open
Viral tracers that permit efficient retrograde targeting of projection neurons are powerful vehicles for structural and functional dissections of the neural circuit and for the treatment of brain diseases. Recombinant adeno-associated viru…
View article: A rabies virus– based toolkit for efficient retrograde labeling and monosynaptic tracing
A rabies virus– based toolkit for efficient retrograde labeling and monosynaptic tracing Open
Analyzing the structure and function of the brain's neural network is critical for identifying the working principles of the brain and the mechanisms of brain diseases. Recombinant rabies viral vectors allow for the retrograde labeling of …
View article: Popularizing recombinant baculovirus-derived OneBac system for scaling-up production of all recombinant adeno-associated virus vector serotypes
Popularizing recombinant baculovirus-derived OneBac system for scaling-up production of all recombinant adeno-associated virus vector serotypes Open
Recombinant adeno-associated virus (rAAV) has been widely used as an efficient transgenic vector in biomedical research, as well as gene therapy. Serotype-associated transduction efficiency, tissue- or cell-type tropism and immunological p…
View article: High-brightness anterograde transneuronal HSV1 H129 tracer modified using a Trojan horse-like strategy
High-brightness anterograde transneuronal HSV1 H129 tracer modified using a Trojan horse-like strategy Open
View article: Development of Versatile and Flexible Sf9 Packaging Cell Line-Dependent OneBac System for Large-Scale Recombinant Adeno-Associated Virus Production
Development of Versatile and Flexible Sf9 Packaging Cell Line-Dependent OneBac System for Large-Scale Recombinant Adeno-Associated Virus Production Open
Recombinant adeno-associated viruses (rAAVs) are excellent vectors for gene delivery. However, current Sf9/Cap-Rep packaging cell line-dependent OneBac systems still lack versatility and flexibility for large-scale production of rAAVs. In …
View article: A Recombinant Baculovirus Efficiently Generates Recombinant Adeno-Associated Virus Vectors in Cultured Insect Cells and Larvae
A Recombinant Baculovirus Efficiently Generates Recombinant Adeno-Associated Virus Vectors in Cultured Insect Cells and Larvae Open
Current large-scale recombinant adeno-associated virus (rAAV) production systems based on the baculovirus expression vector (BEV) remain complicated and cost-intensive, and they lack versatility and flexibility. Here we present a novel rec…
View article: Functional annotation of Alzheimer's disease associated loci revealed by GWASs
Functional annotation of Alzheimer's disease associated loci revealed by GWASs Open
Genome-wide association studies (GWASs) discovered a number of SNPs and genes associated with Alzheimer's disease (AD). However, how these SNPs and genes influence the liability to AD is not fully understood. We deployed computational appr…