Zhenglun Liang
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View article: Generating Synthetic Multi-national Longitudinal Cohorts for Clinically Grounded HIV Research
Generating Synthetic Multi-national Longitudinal Cohorts for Clinically Grounded HIV Research Open
High-quality, widely accessible international longitudinal cohort data for people living with HIV (PWH) have long been needed for advancing open science and data-driven innovation, yet stringent and incongruent privacy regulations have mad…
View article: Optimization of In Vitro Transcription by Design of Experiment to Achieve High Self-Amplifying RNA Integrity
Optimization of In Vitro Transcription by Design of Experiment to Achieve High Self-Amplifying RNA Integrity Open
Background: Self-amplifying mRNA (saRNA) holds promising application prospects. However, due to the inclusion of a replicase sequence, its extended length leads to premature termination during in vitro transcription (IVT), resulting in poo…
View article: Hijacking the Host Cell for Replication: Pro-Viral Host Factors Involved in EVA71 Infection
Hijacking the Host Cell for Replication: Pro-Viral Host Factors Involved in EVA71 Infection Open
Enterovirus A71 (EVA71) is a major pathogen that causes hand, foot, and mouth disease (HFMD). Although the symptoms of HFMD can be self-limiting, severe meningitis, encephalitis, myocarditis, and acute flaccid paralysis may occur. Upon EVA…
View article: Broad-spectrum coronavirus vaccines: integrated strategies to combat viral diversity
Broad-spectrum coronavirus vaccines: integrated strategies to combat viral diversity Open
Prioritizing Betacoronavirus-focused vaccines may provide practical near-term solutions, while long-term success hinges on integrating AI and structural biology for precision engineering. Future efforts must emphasize durable immunity, muc…
View article: Temporal trends in tuberculosis and time from enrolment in care to antiretroviral therapy initiation: A multicountry cohort study from Latin America
Temporal trends in tuberculosis and time from enrolment in care to antiretroviral therapy initiation: A multicountry cohort study from Latin America Open
View article: An attenuated coxsackievirus B5 mutant carrying VP1-N157K retains oncolytic potency against non-small cell lung cancer
An attenuated coxsackievirus B5 mutant carrying VP1-N157K retains oncolytic potency against non-small cell lung cancer Open
View article: HMGA2 interacts with KAT6A to regulate MMPs chromatin architecture and promote triple-negative breast cancer metastasis
HMGA2 interacts with KAT6A to regulate MMPs chromatin architecture and promote triple-negative breast cancer metastasis Open
Background Triple-negative breast cancer (TNBC), the most lethal breast cancer subtype, demonstrates poor prognosis due to its high rates of metastasis, recurrence, and mortality. The metastatic potential in TNBC patients serves as a criti…
View article: Research progress on immune mechanism and control strategy of dsRNA impurities in mRNA vaccine
Research progress on immune mechanism and control strategy of dsRNA impurities in mRNA vaccine Open
dsRNA as a critical quality attribute (CQA), the structural heterogeneity of it (including length and structure) and its precise immunomodulatory mechanisms affecting vaccine safety are poorly understood. Regulatory authorities have not re…
View article: Exploring the standardized detection and sampling methods of human nasal SARS-CoV-2 RBD IgA
Exploring the standardized detection and sampling methods of human nasal SARS-CoV-2 RBD IgA Open
Introduction Vaccines capable of effectively inducing mucosal immunity, particularly specific IgA antibodies, represent an ideal strategy for preventing infections and the transmission of pathogens such as SARS-CoV-2 and influenza viruses …
View article: Development of In Vitro Potency Methods to Replace In Vivo Tests for Enterovirus 71 Inactivated Vaccine (Human Diploid Cell-Based/Vero Cell-Based)
Development of In Vitro Potency Methods to Replace In Vivo Tests for Enterovirus 71 Inactivated Vaccine (Human Diploid Cell-Based/Vero Cell-Based) Open
Background: The three commercial Enterovirus 71 (EV71) inactivated vaccines which have effectively controlled the EV71 pandemic currently rely on inherent variable in vivo potency methods for batch release. To align with 3R (Replacement, R…
View article: Effectiveness of a booster dose of aerosolized or intramuscular adenovirus type 5 vectored COVID-19 vaccine in adults: a multicenter, partially randomized, platform trial in China
Effectiveness of a booster dose of aerosolized or intramuscular adenovirus type 5 vectored COVID-19 vaccine in adults: a multicenter, partially randomized, platform trial in China Open
View article: Four-Color Pseudovirus-Based Neutralization Assay: A Rapid Method for Evaluating Neutralizing Antibodies Against Quadrivalent Hand, Foot, and Mouth Disease Vaccine
Four-Color Pseudovirus-Based Neutralization Assay: A Rapid Method for Evaluating Neutralizing Antibodies Against Quadrivalent Hand, Foot, and Mouth Disease Vaccine Open
Background/Objectives: Enterovirus 71 (EV71) and coxsackieviruses A16 (CA16), A10 (CA10), and A6 (CA6) are the primary pathogens that cause hand, foot, and mouth disease (HFMD). Currently, many manufacturers are developing bivalent, trival…
View article: Exploring the standardization of human nasal antibody measurements
Exploring the standardization of human nasal antibody measurements Open
Mucosal immunity is crucial for preventing the infection and transmission of respiratory viruses. Nasal antibody is inversely correlated with a lower risk of infection with respiratory viruses. However, the current reference standard for n…
View article: Temporal Trends in Tuberculosis and the Time from Enrollment in Care to Antiretroviral Therapy Initiation Among People Living with HIV in Latin America
Temporal Trends in Tuberculosis and the Time from Enrollment in Care to Antiretroviral Therapy Initiation Among People Living with HIV in Latin America Open
View article: Anti-Tumor Analysis of the Rig-I Agonist in Vitro and in Vivo
Anti-Tumor Analysis of the Rig-I Agonist in Vitro and in Vivo Open
View article: Study on the Characterization and Degradation Pattern of Circular RNA Vaccines Using an HPLC Method
Study on the Characterization and Degradation Pattern of Circular RNA Vaccines Using an HPLC Method Open
Circular RNA (circRNA) vaccines have attracted increasing attention due to their stable closed-loop structures and persistent protein expression ability. During the synthesis process, nicked circRNAs with similar molecular weights to those…
View article: Research progress on the quality control of mRNA vaccines
Research progress on the quality control of mRNA vaccines Open
Although there has been rapid progress in research on linear mRNA vaccines, their degradation patterns remain unclear. In addition, standardized assays for key impurities, such as residual dsRNA and T7 RNA polymerase, are still lacking. Fo…
View article: Development and application of a high-sensitivity immunochromatographic test strip for detecting pseudorabies virus
Development and application of a high-sensitivity immunochromatographic test strip for detecting pseudorabies virus Open
Introduction Pseudorabies (PR) is a multi-animal comorbid disease caused by pseudorabies virus (PRV), which are naturally found in pigs. At the end of 2011, the emergence of PRV variant strains in many provinces in China had caused huge ec…
View article: Establishment of the First National Standard for Neutralizing Antibodies against SARS-CoV-2 XBB Variants
Establishment of the First National Standard for Neutralizing Antibodies against SARS-CoV-2 XBB Variants Open
Neutralizing antibodies (NtAbs) against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) are indicators of vaccine efficacy that enable immunity surveillance. However, the rapid mutation of SARS-CoV-2 variants prevents the time…
View article: A Cocktail of Lipid Nanoparticle-mRNA Vaccines Broaden Immune Responses against β-Coronaviruses in a Murine Model
A Cocktail of Lipid Nanoparticle-mRNA Vaccines Broaden Immune Responses against β-Coronaviruses in a Murine Model Open
Severe acute respiratory syndrome (SARS)-coronavirus (CoV), Middle Eastern respiratory syndrome (MERS)-CoV, and SARS-CoV-2 have seriously threatened human life in the 21st century. Emerging and re-emerging β-coronaviruses after the coronav…
View article: Progress and challenges in the clinical evaluation of immune responses to respiratory mucosal vaccines
Progress and challenges in the clinical evaluation of immune responses to respiratory mucosal vaccines Open
Introduction Following the coronavirus disease pandemic, respiratory mucosal vaccines that elicit both mucosal and systemic immune responses have garnered increasing attention. However, human physiological characteristics pose significant …
View article: A screening study on the detection strain of Coxsackievirus A6: the key to evaluating neutralizing antibodies in vaccines
A screening study on the detection strain of Coxsackievirus A6: the key to evaluating neutralizing antibodies in vaccines Open
The increasing incidence of diseases caused by Coxsackievirus A6 (CV-A6) and the presence of various mutants in the population present significant public health challenges. Given the concurrent development of multiple vaccines in China, it…
View article: Amplifying mRNA vaccines: potential versatile magicians for oncotherapy
Amplifying mRNA vaccines: potential versatile magicians for oncotherapy Open
Cancer vaccines drive the activation and proliferation of tumor-reactive immune cells, thereby eliciting tumor-specific immunity that kills tumor cells. Accordingly, they possess immense potential in cancer treatment. However, such vaccine…
View article: Non-small cell lung cancers (NSCLCs) oncolysis using coxsackievirus B5 and synergistic DNA-damage response inhibitors
Non-small cell lung cancers (NSCLCs) oncolysis using coxsackievirus B5 and synergistic DNA-damage response inhibitors Open
View article: A Novel Targeted RIG-I Receptor 5′Triphosphate Double Strain RNA-Based Adjuvant Significantly Improves the Immunogenicity of the SARS-CoV-2 Delta-Omicron Chimeric RBD-Dimer Recombinant Protein Vaccine
A Novel Targeted RIG-I Receptor 5′Triphosphate Double Strain RNA-Based Adjuvant Significantly Improves the Immunogenicity of the SARS-CoV-2 Delta-Omicron Chimeric RBD-Dimer Recombinant Protein Vaccine Open
The rapid mutation and spread of SARS-CoV-2 variants recently, especially through the emerging variants Omicron BA5, BF7, XBB and BQ1, necessitate the development of universal vaccines to provide broad spectrum protection against variants.…
View article: Standardized neutralization antibody analytical procedure for clinical samples based on the AQbD concept
Standardized neutralization antibody analytical procedure for clinical samples based on the AQbD concept Open
View article: Research Advances on the Stability of mRNA Vaccines
Research Advances on the Stability of mRNA Vaccines Open
Compared to other vaccines, the inherent properties of messenger RNA (mRNA) vaccines and their interaction with lipid nanoparticles make them considerably unstable throughout their life cycles, impacting their effectiveness and global acce…
View article: Establishment of national standard for anti-SARS-Cov-2 neutralizing antibody in China: The first National Standard calibration traceability to the WHO International Standard
Establishment of national standard for anti-SARS-Cov-2 neutralizing antibody in China: The first National Standard calibration traceability to the WHO International Standard Open
Neutralizing antibody (NtAb) levels are key indicators in the development and evaluation of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) vaccines. Establishing a unified and reliable WHO International Standard (IS) for NtAb…
View article: Research progress on substitution of <i>in vivo</i> method(s) by <i>in vitro</i> method(s) for human vaccine potency assays
Research progress on substitution of <i>in vivo</i> method(s) by <i>in vitro</i> method(s) for human vaccine potency assays Open
Owing to the variability of in vivo methods, the correlation between in vivo and in vitro methods may be low. One or more in vitro method(s) that determine the vaccine antigen content and functions, should be es…
View article: Research progress on circular RNA vaccines
Research progress on circular RNA vaccines Open
Owing to the success of linear mRNA coronavirus disease 2019 (COVID-19) vaccines, biopharmaceutical companies and research teams worldwide have attempted to develop more stable circular RNA (circRNA) vaccines and have achieved some prelimi…