Zhenlin Ouyang
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View article: Structural and mechanistic insights into the transcriptional regulation of chromosomal T6SS by large conjugative plasmid-encoded TetRs in <i>Acinetobacter baumannii</i>
Structural and mechanistic insights into the transcriptional regulation of chromosomal T6SS by large conjugative plasmid-encoded TetRs in <i>Acinetobacter baumannii</i> Open
The type VI secretion system (T6SS) is a highly sophisticated nanomachine widely used by bacteria to achieve competitive advantage, important for survival and pathogenicity. In Acinetobacter baumannii, the large conjugative plasmid pAB3 en…
View article: Molybdate uptake interplay with ROS tolerance modulates bacterial pathogenesis
Molybdate uptake interplay with ROS tolerance modulates bacterial pathogenesis Open
The rare metal element molybdenum functions as a cofactor in molybdoenzymes that are essential to life in almost all living things. Molybdate can be captured by the periplasmic substrate-binding protein ModA of ModABC transport system in b…
View article: The Functional Study of Response Regulator ArlR Mutants in Staphylococcus Aureus
The Functional Study of Response Regulator ArlR Mutants in Staphylococcus Aureus Open
Staphylococcus aureus is a major cause of hospital-associated infections worldwide. The organism’s ability to form biofilms has led to resistance against current treatment options such as beta-lactams, glycopeptides, and daptomycin. The Ar…
View article: Mechanistic and biophysical characterization of polymyxin resistance response regulator PmrA in Acinetobacter baumannii
Mechanistic and biophysical characterization of polymyxin resistance response regulator PmrA in Acinetobacter baumannii Open
Introduction Acinetobacter baumannii PmrAB is a crucial two-component regulatory system (TCS) that plays a vital role in conferring resistance to polymyxin. PmrA, a response regulator belonging to the OmpR/PhoB family, is composed of a C-t…
View article: Structure and assembly of type VI secretion system cargo delivery vehicle
Structure and assembly of type VI secretion system cargo delivery vehicle Open
Type VI secretion system is widely used in Gram-negative bacteria for injecting toxic effectors into neighboring prokaryotic or eukaryotic cells. Various effectors can be loaded onto the T6SS delivery tube via its core components: Hcp, Vgr…
View article: Screening and epitope characterization of diagnostic nanobody against total and activated Bacteroides fragilis toxin
Screening and epitope characterization of diagnostic nanobody against total and activated Bacteroides fragilis toxin Open
Enterotoxigenic Bacteroides fragilis (ETBF) can rapidly secrete an enterotoxin termed B. fragilis toxin (BFT), which is thought to be the only recognized virulence factor in ETBF. ETBF can cause acute diarrhea, inflammatory bowel disease (…
View article: Progress in structural and functional study of the bacterial phenylacetic acid catabolic pathway, its role in pathogenicity and antibiotic resistance
Progress in structural and functional study of the bacterial phenylacetic acid catabolic pathway, its role in pathogenicity and antibiotic resistance Open
Phenylacetic acid (PAA) is a central intermediate metabolite involved in bacterial degradation of aromatic components. The bacterial PAA pathway mainly contains 12 enzymes and a transcriptional regulator, which are involved in biofilm form…
View article: An Outer Membrane Protein YiaD Contributes to Adaptive Resistance of Meropenem in Acinetobacter baumannii
An Outer Membrane Protein YiaD Contributes to Adaptive Resistance of Meropenem in Acinetobacter baumannii Open
Acinetobacter baumannii can develop various resistance mechanisms to carbapenems. However, the factors involved in the evolutionary process that leads from transition to the sensitive to resistant phenotype are not clear.
View article: Development and Characterization of Nanobodies Targeting the Kupffer Cell
Development and Characterization of Nanobodies Targeting the Kupffer Cell Open
Nanobodies that are derived from single-chain antibodies of camelids have served as powerful tools in diagnostics, therapeutics and investigation of membrane receptors' structure and function. In this study, we developed a series of nanobo…
View article: CIC Is a Mediator of the ERK1/2-DUSP6 Negative Feedback Loop
CIC Is a Mediator of the ERK1/2-DUSP6 Negative Feedback Loop Open
DUSP6 functions as an important negative feedback component of the MAPK/ERK signaling pathway. Although DUSP6 expression is tightly regulated by ERK1/2 signaling, the molecular mechanism of this regulation remains partially understood. In …
View article: Deciphering the activation and recognition mechanisms of Staphylococcus aureus response regulator ArlR
Deciphering the activation and recognition mechanisms of Staphylococcus aureus response regulator ArlR Open
Staphylococcus aureus ArlRS is a key two-component regulatory system necessary for adhesion, biofilm formation, and virulence. The response regulator ArlR consists of a C-terminal DNA-binding effector domain and an N-terminal receiver doma…
View article: Trimeric structure of the mouse Kupffer cell C‐type lectin receptor Clec4f
Trimeric structure of the mouse Kupffer cell C‐type lectin receptor Clec4f Open
The C‐type lectin receptor Clec4f has been identified as a specific surface marker for Kupffer cells, although its ortholog is absent in humans and its biological function remains elusive. Here, we report the crystal structure of a truncat…
View article: NIRF-Molecular Imaging with Synovial Macrophages-Targeting Vsig4 Nanobody for Disease Monitoring in a Mouse Model of Arthritis
NIRF-Molecular Imaging with Synovial Macrophages-Targeting Vsig4 Nanobody for Disease Monitoring in a Mouse Model of Arthritis Open
Nanobody against V-set and Ig domain-containing 4 (Vsig4) on tissue macrophages, such as synovial macrophages, could visualize joint inflammation in multiple experimental arthritis models via single-photon emission computed tomography imag…
View article: ERK-mediated phosphorylation regulates SOX10 sumoylation and targets expression in mutant BRAF melanoma
ERK-mediated phosphorylation regulates SOX10 sumoylation and targets expression in mutant BRAF melanoma Open
In human mutant BRAF melanoma cells, the stemness transcription factor FOXD3 is rapidly induced by inhibition of ERK1/2 signaling and mediates adaptive resistance to RAF inhibitors. However, the mechanism underlying ERK signaling control o…
View article: Crystal structure of master biofilm regulator CsgD regulatory domain reveals an atypical receiver domain
Crystal structure of master biofilm regulator CsgD regulatory domain reveals an atypical receiver domain Open
The master regulator CsgD switches planktonic growth to biofilm formation by activating synthesis of curli fimbriae and cellulose in Enterobacteriaceae. CsgD was classified to be the LuxR response regulatory family, while its cognate senso…
View article: Mechanistic insight into how multidrug resistant Acinetobacter baumannii response regulator AdeR recognizes an intercistronic region
Mechanistic insight into how multidrug resistant Acinetobacter baumannii response regulator AdeR recognizes an intercistronic region Open
AdeR-AdeS is a two-component regulatory system, which controls expression of the adeABC efflux pump involved in Acinetobacter baumannii multidrug resistance. AdeR is a response regulator consisting of an N-terminal receiver domain and a C-…