Zilei Liu
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View article: Characterizing Foxp3+ and Foxp3- T cells in the homeostatic state and after allo-activation: resting CD4+Foxp3+ Tregs have molecular characteristics of activated T cells
Characterizing Foxp3+ and Foxp3- T cells in the homeostatic state and after allo-activation: resting CD4+Foxp3+ Tregs have molecular characteristics of activated T cells Open
Due to the intracellular expression of Foxp3 it is impossible to purify viable Foxp3 + cells on the basis of Foxp3 staining. Consequently CD4 + Foxp3 + regulatory T cells (Tregs) in mice have mostly been characterized using CD4 + CD25 + T …
View article: Detecting Tumor Specific Antigen-Reactive T cells from Tumor Infiltrating Lymphocytes via Interaction Dependent Fucosyl-biotinylation
Detecting Tumor Specific Antigen-Reactive T cells from Tumor Infiltrating Lymphocytes via Interaction Dependent Fucosyl-biotinylation Open
Summary Re-activation and clonal expansion of tumor specific antigen (TSA)-reactive T cells are critical to the success of checkpoint blockade and adoptive transfer of tumor-infiltrating lymphocyte (TIL) based therapies. There are no relia…
View article: d-Idose, d-Iduronic Acid, and d-Idonic Acid from d-Glucose via Seven-Carbon Sugars
d-Idose, d-Iduronic Acid, and d-Idonic Acid from d-Glucose via Seven-Carbon Sugars Open
A practical synthesis of the very rare sugar d-idose and the stable building blocks for d-idose, d-iduronic, and d-idonic acids from ido-heptonic acid requires only isopropylidene protection, Shing silica gel-supported periodate cleavage o…
View article: Novel Approaches to Access Arylfluorosulfates and Sulfamoyl Fluorides Based on Sulfur (VI) Fluoride Exchange
Novel Approaches to Access Arylfluorosulfates and Sulfamoyl Fluorides Based on Sulfur (VI) Fluoride Exchange Open
Sulfur (VI) fluoride exchange (SuFEx) is a new family of click chemistry reactions that relies on readily available sulfuryl fluoride (SO 2 F 2 ) and ethenesulfonyl fluoride to build diverse chemical structures bearing the S VI ‐F motif, s…
View article: A Single-Step Chemoenzymatic Reaction for the Construction of Antibody–Cell Conjugates
A Single-Step Chemoenzymatic Reaction for the Construction of Antibody–Cell Conjugates Open
Employing live cells as therapeutics is a direction of future drug discovery. An easy and robust method to modify the surfaces of cells directly to incorporate novel functionalities is highly desirable. However, genetic methods for cell-su…
View article: Single-step Enzymatic Glycoengineering for the Construction of Antibody-cell Conjugates
Single-step Enzymatic Glycoengineering for the Construction of Antibody-cell Conjugates Open
Employing live cells as therapeutics is a direction of future drug discovery. An easy and robust method to modify the surfaces of cells directly to incorporate novel functionalities is highly desirable. However, many current methods for ce…
View article: SuFEx Click Chemistry Enabled Late-Stage Drug Functionalization
SuFEx Click Chemistry Enabled Late-Stage Drug Functionalization Open
Sulfur(VI) Fluoride Exchange (SuFEx) is a new family of click chemistry transformations which relies on readily available materials to produce compounds bearing the SVI-F motif. The potential of SuFEx in drug discovery has just started to …
View article: 6‐Deoxyhexoses from <scp>l</scp>‐Rhamnose in the Search for Inducers of the Rhamnose Operon: Synergy of Chemistry and Biotechnology
6‐Deoxyhexoses from <span>l</span>‐Rhamnose in the Search for Inducers of the Rhamnose Operon: Synergy of Chemistry and Biotechnology Open
In the search for alternative non‐metabolizable inducers in the l ‐rhamnose promoter system, the synthesis of fifteen 6‐deoxyhexoses from l ‐rhamnose demonstrates the value of synergy between biotechnology and chemistry. The readily availa…
View article: Synthetic Chemical Inducers and Genetic Decoupling Enable Orthogonal Control of the <i>rhaBAD</i> Promoter
Synthetic Chemical Inducers and Genetic Decoupling Enable Orthogonal Control of the <i>rhaBAD</i> Promoter Open
External control of gene expression is crucial in synthetic biology and biotechnology research and applications, and is commonly achieved using inducible promoter systems. The E. coli rhamnose-inducible rhaBAD promoter has properties super…
View article: Synthesis and biological evaluation of rare monosaccharides and their mimics
Synthesis and biological evaluation of rare monosaccharides and their mimics Open
This thesis describes work in the synthesis and biological evaluation of different carbohydrates and their derivatives. Azetidine carboxylic acid (Aze), as a pharmacophore in medicinal chemistry, is attracting more and more researchers in …
View article: 3-Fluoroazetidinecarboxylic Acids and <i>trans,trans-</i>3,4-Difluoroproline as Peptide Scaffolds: Inhibition of Pancreatic Cancer Cell Growth by a Fluoroazetidine Iminosugar
3-Fluoroazetidinecarboxylic Acids and <i>trans,trans-</i>3,4-Difluoroproline as Peptide Scaffolds: Inhibition of Pancreatic Cancer Cell Growth by a Fluoroazetidine Iminosugar Open
Reverse aldol opening renders amides of 3-hydroxyazetidinecarboxylic acids (3-OH-Aze) unstable above pH 8. Aze, found in sugar beet, is mis-incorporated for proline in peptides in humans and is associated with multiple sclerosis and terato…