Zoë L. Wilson YOU? Author Swipe

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Saturation Mutagenesis Genome Engineering of Infective ΦX174 Bacteriophage <i>via</i> Unamplified Oligo Pools and Golden Gate Assembly Open

Matthew S. Faber, James T. Van Leuven, Martina M. Ederer, Yesol Sapozhnikov, Zoë L. Wilson , et al. · 2019

Here we present a novel protocol for the construction of saturation single-site-and massive multisite-mutant libraries of a bacteriophage. We segmented the ΦX174 genome into 14 nontoxic and nonreplicative fragments compatible with Golden G…

Saturation mutagenesis genome engineering of infective ΦX174 bacteriophage via unamplified oligo pools and golden gate assembly Open

Matthew S. Faber, James T. Van Leuven, Martina M. Ederer, Yesol Sapozhnikov, Zoë L. Wilson , et al. · 2019

Here we present a novel protocol for the construction of saturation single-site—and massive multi-site—mutant libraries of a bacteriophage. We segmented the ΦX174 genome into 14 non-toxic and non-replicative fragments compatible with golde…