Micropatterning
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A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures Open
The biological performance of artificial biomaterials is closely related to their structure characteristics. Cell adhesion, migration, proliferation, and differentiation are all strongly affected by the different scale structures of biomat…
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Regulation of nuclear architecture, mechanics, and nucleocytoplasmic shuttling of epigenetic factors by cell geometric constraints Open
Significance Growing evidence suggests that cytoskeleton-mediated transmission of mechanical forces from the extracellular matrix (ECM) to the nucleus alters nuclear mechanics and architecture and these alterations can, in turn, impact the…
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Droplet‐Array (DA) Sandwich Chip: A Versatile Platform for High‐Throughput Cell Screening Based on Superhydrophobic–Superhydrophilic Micropatterning Open
A droplet-array (DA) sandwich chip is a miniaturized platform for cell-based high-throughput screening. It is based on sandwiching of a glass slide with a preprinted library and a superhydrophobic-superhydrophilic pattern, which consists o…
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Bridging the Gap: From 2D Cell Culture to 3D Microengineered Extracellular Matrices Open
Historically the culture of mammalian cells in the laboratory has been performed on planar substrates with media cocktails that are optimized to maintain phenotype. However, it is becoming increasingly clear that much of biology discerned …
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Low-cost and cleanroom-free fabrication of microneedles Open
We present a facile, low-cost and cleanroom-free technique for the fabrication of microneedles using molds created by laser ablation. Microneedle mold with high aspect ratios is achieved on acrylic sheet by engraving a specific pattern of …
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Surface acoustic waves induced micropatterning of cells in gelatin methacryloyl (GelMA) hydrogels Open
Acoustic force patterning is an emerging technology that provides a platform to control the spatial location of cells in a rapid, accurate, yet contactless manner. However, very few studies have been reported on the usage of acoustic force…
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Simultaneous Micropatterning of Fibrous Meshes and Bioinks for the Fabrication of Living Tissue Constructs Open
Fabrication of biomimetic tissues holds much promise for the regeneration of cells or organs that are lost or damaged due to injury or disease. To enable the generation of complex, multicellular tissues on demand, the ability to design and…
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Laser-based three-dimensional multiscale micropatterning of biocompatible hydrogels for customized tissue engineering scaffolds Open
Significance In this paper we present results on 3D, multiscale laser machining of soft, transparent biomaterials suited for cellular growth and/or implantation. We use an ultrafast laser to generate high-resolution, 3D structures within t…
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Universal three-dimensional crosslinker for all-photopatterned electronics Open
All-solution processing of large-area organic electronics requires multiple steps of patterning and stacking of various device components. Here, we report the fabrication of highly integrated arrays of polymer thin-film transistors and log…
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Micropatterning of cells via adjusting surface wettability using plasma treatment and graphene oxide deposition Open
The wettability of a polymer surface plays a critical role in cell-cell interaction and behavior. The degree to which a surface is hydrophobic or hydrophilic affects the adhesion and behavior of cells. Two distinct techniques for patternin…
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Photo Processing for Biomedical Hydrogels Design and Functionality: A Review Open
A large number of opportunities for biomedical hydrogel design and functionality through photo-processing have stretched the limits of innovation. As both photochemical understanding and engineering technologies continue to develop, more c…
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Freeform micropatterning of living cells into cell culture medium using direct inkjet printing Open
Microfabrication methods have widely been used to control the local cellular environment on a micron scale. However, accurately mimicking the complexity of the in vivo tissue architecture while maintaining the freedom of form and design is…
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Micropatterning of TCR and LFA-1 ligands reveals complementary effects on cytoskeleton mechanics in T cells Open
Micropatterning of T cell ligands reveals their specific contributions to actin assembly and the generation of cytoskeletal forces in immunesynapse formation.
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Micropatterning Strategies to Engineer Controlled Cell and Tissue Architecture in Vitro Open
Micropatterning strategies, which enable control over cell and tissue architecture in vitro, have emerged as powerful platforms for modelling tissue microenvironments at different scales and complexities. Here, we provide an overview of po…
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Spatial distribution of cell–cell and cell–ECM adhesions regulates force balance while maintaining E-cadherin molecular tension in cell pairs Open
Mechanical linkage between cell–cell and cell–extracellular matrix (ECM) adhesions regulates cell shape changes during embryonic development and tissue homoeostasis. We examined how the force balance between cell–cell and cell–ECM adhesion…
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Mapping intracellular mechanics on micropatterned substrates Open
Significance Cell mechanics is crucial for many, if not all, cell functions. Although mechanics at the scale of the whole cell is extensively documented, there are only few methods to measure intracellular mechanics at the local scale. Her…
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Epigenetically heterogeneous tumor cells direct collective invasion through filopodia-driven fibronectin micropatterning Open
Specialized leader cells use stable filopodia to create linear fibronectin tracks to promote collective cancer invasion.
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Inkjet printing of mammalian cells – Theory and applications Open
Inkjet bioprinting is a new and versatile technology which has found novel applications in cell biology and associated biomedical research. Cells suspended in a low-viscosity liquid medium can be readily dispensed using piezoelectric and t…
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Methods of Micropatterning and Manipulation of Cells for Biomedical Applications Open
Micropatterning and manipulation of mammalian and bacterial cells are important in biomedical studies to perform in vitro assays and to evaluate biochemical processes accurately, establishing the basis for implementing biomedical microelec…
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Tailored Graphene Micropatterns by Wafer‐Scale Direct Transfer for Flexible Chemical Sensor Platform Open
2D materials, such as graphene, exhibit great potential as functional materials for numerous novel applications due to their excellent properties. The grafting of conventional micropatterning techniques on new types of electronic devices i…
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Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells Open
Efficient translation of human induced pluripotent stem cells (hiPSCs) requires scalable cell manufacturing strategies for optimal self-renewal and functional differentiation. Traditional manual cell culture is variable and labor intensive…
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3D cardiac μtissues within a microfluidic device with real-time contractile stress readout Open
We present the development of three-dimensional (3D) cardiac microtissues within a microfluidic device with the ability to quantify real-time contractile stress measurementsin situ.
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Aluminum nanostructures with strong visible-range SERS activity for versatile micropatterning of molecular security labels Open
A ciphertext-embedded machine-readable micro-QR code is constructed using structurally uniform visible-range SERS-active Al nanostructures with an average SERS enhancement factor of >104.
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Micropatterned Hydrogel Surface with High-Aspect-Ratio Features for Cell Guidance and Tissue Growth Open
Surface topography has been introduced as a new tool to coordinate cell selection, growth, morphology, and differentiation. The materials explored so far for making such structural surfaces are mostly rigid and impermeable. Hydrogel, on th…
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Click-crosslinkable and photodegradable gelatin hydrogels for cytocompatible optical cell manipulation in natural environment Open
This paper describes the generation of “click-crosslinkable“ and “photodegaradable“ gelatin hydrogels from the reaction between dibenzocycloctyl-terminated photoclevable tetra-arm polyethylene glycol and azide-modified gelatin. The hydroge…
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Cell chirality: emergence of asymmetry from cell culture Open
Increasing evidence suggests that intrinsic cell chirality significantly contributes to the left–right (LR) asymmetry in embryonic development, which is a well-conserved characteristic of living organisms. With animal embryos, several theo…
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Micropatterned Scaffolds with Immobilized Growth Factor Genes Regenerate Bone and Periodontal Ligament‐Like Tissues Open
Periodontal disease destroys supporting structures of teeth. However, tissue engineering strategies offer potential to enhance regeneration. Here, the strategies of patterned topography, spatiotemporally controlled growth factor gene deliv…
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Cells‐Micropatterning Biomaterials for Immune Activation and Bone Regeneration Open
Natural tissues are composed of ordered architectural organizations of multiple tissue cells. The spatial distribution of cells is crucial for directing cellular behavior and maintaining tissue homeostasis and function. Herein, an artifici…
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Photopolymerizable Zwitterionic Polymer Patterns Control Cell Adhesion and Guide Neural Growth Open
Developing materials that reduce or eliminate fibrosis encapsulation of neural prosthetic implants could significantly enhance implant fidelity by improving the tissue/electrode array interface. Here, we report on the photografting and pat…
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Cytoskeletal tension regulates mesodermal spatial organization and subsequent vascular fate Open
Significance Human-induced pluripotent stem cells (hiPSCs) offer an opportunity to study how the developing embryo twists, folds, and expands into a collection of highly self-organized tissues. Introducing varying degrees of confinement wi…