Dynein
View article: Hierarchical Motor Control of Cytoskeletal Architecture Dictates Cell Mechanics
Hierarchical Motor Control of Cytoskeletal Architecture Dictates Cell Mechanics Open
Cell mechanics, encompassing properties like stiffness, tension, and viscoelasticity, are fundamental to crucial biological processes including cell migration, division, differentiation, and tissue morphogenesis. These mechanical propertie…
View article: Hierarchical Motor Control of Cytoskeletal Architecture Dictates Cell Mechanics
Hierarchical Motor Control of Cytoskeletal Architecture Dictates Cell Mechanics Open
Cell mechanics, encompassing properties like stiffness, tension, and viscoelasticity, are fundamental to crucial biological processes including cell migration, division, differentiation, and tissue morphogenesis. These mechanical propertie…
View article: Molecular motors orchestrate pause-and-run dynamics to facilitate intracellular transport
Molecular motors orchestrate pause-and-run dynamics to facilitate intracellular transport Open
Intracellular transport is essential for cellular organization and function. This process is driven by molecular motors that ferry cargo along microtubules, but is characterized by intermittent motility, where cargoes switch between direct…
View article: Human dynein-dynactin is a fast processive motor in living cells
Human dynein-dynactin is a fast processive motor in living cells Open
Minus-end directed transport along microtubules in eukaryotes is primarily mediated by cytoplasmic dynein and its cofactor dynactin. Significant advances have been made in recent years characterizing human dynein-dynactin structure and fun…
View article: Joubert syndrome 26 protein enforces compartmentalized motility of a ciliary kinesin
Joubert syndrome 26 protein enforces compartmentalized motility of a ciliary kinesin Open
Cilia are essential cellular antennae that rely on precise motor-driven transport to assemble and function. Two kinesin-2 motors—kinesin-II and OSM-3 in Caenorhabditis elegans —cooperate to transport cargo along cilia, with kinesin-II oper…
View article: NuMA and Ninein: Dynein Cargo-Adaptors Without a Classical Cargo
NuMA and Ninein: Dynein Cargo-Adaptors Without a Classical Cargo Open
Dynein is a minus-end-directed microtubule motor that transports a variety of cargoes. Cargo specificity is mediated by a class of adaptor proteins that bind to the interface between dynein and dynactin, along the length of the Arp1 filame…
View article: Physical and functional interaction of Lrrc56 and Odad3 is required for deployment of axonemal dyneins in vertebrate multiciliated cells
Physical and functional interaction of Lrrc56 and Odad3 is required for deployment of axonemal dyneins in vertebrate multiciliated cells Open
Primary ciliary dyskinesia (PCD) is a genetically heterogeneous motile ciliopathy characterized by chronic respiratory disease, laterality defects, hydrocephalus, and infertility, caused by impaired function of motile cilia. LRRC56 has rec…
View article: A conserved minimal core and modular extensions make the fungal flagellum
A conserved minimal core and modular extensions make the fungal flagellum Open
Flagellated fungi are pivotal for understanding the evolution of eukaryotic motility, yet knowledge of their flagellar proteins remains fragmentary. We assembled a cross-phyla atlas by mapping 303 reference flagellar proteins on 184 fungal…
View article: DNA tensiometer reveals catch-bond detachment kinetics of kinesin-1, -2 and -3
DNA tensiometer reveals catch-bond detachment kinetics of kinesin-1, -2 and -3 Open
Bidirectional cargo transport by kinesin and dynein is essential for cell viability and defects are linked to neurodegenerative diseases. Computational modeling suggests that the load-dependent off-rate is the strongest determinant of whic…
View article: In vitro motility-based tether-scanning of the kinesin motor domain
In vitro motility-based tether-scanning of the kinesin motor domain Open
View article: DNA tensiometer reveals catch-bond detachment kinetics of kinesin-1, -2 and -3
DNA tensiometer reveals catch-bond detachment kinetics of kinesin-1, -2 and -3 Open
Bidirectional cargo transport by kinesin and dynein is essential for cell viability and defects are linked to neurodegenerative diseases. Computational modeling suggests that the load-dependent off-rate is the strongest determinant of whic…
View article: <i>Arabidopsis</i> <scp>AUGMIN8</scp> Contains Two Independent Microtubule Association Domains
<i>Arabidopsis</i> <span>AUGMIN8</span> Contains Two Independent Microtubule Association Domains Open
Plant cells create a plasma membrane‐associated network of microtubules that are nucleated by γ‐tubulin ring complexes primarily through microtubule‐dependent microtubule nucleation (MDMN). This dynamic array organizes into specific patter…
View article: Chemical Approaches to Dynein Inhibition
Chemical Approaches to Dynein Inhibition Open
Cells utilize energy to maintain order within the cytoplasm. Motor proteins are the enzymes that convert the chemical energy contained in adenosine triphosphate (ATP) into directed movement along polarized filaments of actin and tubulin wi…
View article: CFAP300 loss-of-function variant causes primary ciliary dyskinesia and male infertility via disrupting sperm flagellar assembly and acrosome formation
CFAP300 loss-of-function variant causes primary ciliary dyskinesia and male infertility via disrupting sperm flagellar assembly and acrosome formation Open
Primary ciliary dyskinesia (PCD) is a genetically heterogeneous disorder characterized by impaired motility of cilia and flagella. Mutations in cilia- and flagella-associated protein 300 ( CFAP300 ) are associated with human PCD and male i…
View article: <scp>CDKL5</scp> regulates the initiation of retrograde axonal transport through <scp>CLIP170</scp> –dynactin complex formation
<span>CDKL5</span> regulates the initiation of retrograde axonal transport through <span>CLIP170</span> –dynactin complex formation Open
Cyclin‐dependent kinase‐like 5 (CDKL5) is a serine–threonine kinase implicated in regulating microtubule (MT) dynamics. Mutations in CDKL5 are associated with a rare neurodevelopmental disease called CDKL5 deficiency disorder (CDD), which …
View article: Post‐<scp>LECA</scp> Origin and Diversification of an Axonemal Outer Arm Dynein Motor
Post‐<span>LECA</span> Origin and Diversification of an Axonemal Outer Arm Dynein Motor Open
Dyneins were present in the last eukaryotic common ancestor (LECA) and play key roles in eukaryotic biology. Axonemal dyneins form the inner and outer arms that power ciliary beating, and it has long been recognized that outer arms in some…
View article: Characterisation of the manchette architecture and its role as transport scaffold using cryo-electron tomography
Characterisation of the manchette architecture and its role as transport scaffold using cryo-electron tomography Open
The development of correctly shaped sperm cells is crucial for male reproductive health and fertility. The manchette is a transient microtubule-based structure that assembles during spermiogenesis and contributes to sperm head shaping. Def…
View article: NuMA mechanically reinforces the spindle independently of its partner dynein
NuMA mechanically reinforces the spindle independently of its partner dynein Open
View article: Histone lactylation regulates DOCK4 to control heat nociception and supports Dynein-mediated Nav1.7 trafficking
Histone lactylation regulates DOCK4 to control heat nociception and supports Dynein-mediated Nav1.7 trafficking Open
Heat nociception involves thermosensors like transient receptor potential channel V1 in dorsal root ganglion (DRG) neurons, but their loss only partially impairs heat sensing, suggesting other mechanisms. Autism frequently involves abnorma…
View article: Cryo-EM captures early intermediate steps in dynein activation by LIS1
Cryo-EM captures early intermediate steps in dynein activation by LIS1 Open
View article: GPR161 mechanosensitivity at the primary cilium drives neuronal saltatory migration
GPR161 mechanosensitivity at the primary cilium drives neuronal saltatory migration Open
The saltatory migration of neurons is essential for brain formation. Whether mechanical stimuli regulate this process is unknown. Here, we show that the primary cilium acts as a mechanical sensor through GPR161. Using an ex vivo neuronal m…
View article: CDK1-dependent N-terminal NuMA phosphorylation promotes dynein-dynactin-NuMA assembly for accurate chromosome segregation
CDK1-dependent N-terminal NuMA phosphorylation promotes dynein-dynactin-NuMA assembly for accurate chromosome segregation Open
The microtubule-based motor dynein and its cofactor dynactin fulfil essential functions throughout the cell cycle, including organelle transport and mitotic spindle assembly. To achieve these diverse functions, dynein-dynactin associates w…
View article: Dynein‐Dependent Endo‐Lysosomal Degradation Drives Lewy Body Disorders Accompanied by Aβ Pathology
Dynein‐Dependent Endo‐Lysosomal Degradation Drives Lewy Body Disorders Accompanied by Aβ Pathology Open
Dementia with Lewy bodies (DLB) is a significant cause of dementia. However, the limited availability of animal and cellular models that accurately replicate early DLB pathogenesis hampers the understanding of how Aβ plaques influence α‐sy…
View article: Proteomic and structural comparison between cilia from primary ciliary dyskinesia patients with a DNAH5 defect
Proteomic and structural comparison between cilia from primary ciliary dyskinesia patients with a DNAH5 defect Open
Introduction Primary ciliary dyskinesia (PCD) is a genetic disorder affecting motile cilia across various organs, leading to recurrent respiratory infections, subfertility, and laterality defects. While several diagnostic tools exist—such …
View article: Physical and functional interaction of Lrrc56 and Odad3 controls deployment of axonemal dyneins in vertebrate multiciliated cells
Physical and functional interaction of Lrrc56 and Odad3 controls deployment of axonemal dyneins in vertebrate multiciliated cells Open
Primary ciliary dyskinesia is a genetically heterogeneous motile ciliopathy characterized chronic respiratory disease, laterality defects, hydrocephalus, and infertility, caused by impaired function of motile cilia. LRRC56 has recently eme…
View article: The intracellular bacterium Orientia tsutsugamushi uses the autotransporter ScaC to activate BICD adaptors for dynein-based motility
The intracellular bacterium Orientia tsutsugamushi uses the autotransporter ScaC to activate BICD adaptors for dynein-based motility Open
The intracellular bacterium Orientia tsutsugamushi relies on the microtubule cytoskeleton and the motor protein dynein to traffic to the perinuclear region within infected cells. However, it remains unclear how the bacterium is coupled to …
View article: Micron-scale protein transport along microtubules by kinesin-driven shepherding
Micron-scale protein transport along microtubules by kinesin-driven shepherding Open
Microtubule-based long-distance transport in eukaryotic cells typically involves the binding of cargo to motors such as highly processive kinesins for unidirectional transport. An open question is whether long-distance transport can occur …
View article: Dominant spinal muscular atrophy linked mutations in the cargo binding domain of BICD2 result in altered interactomes and dynein hyperactivity
Dominant spinal muscular atrophy linked mutations in the cargo binding domain of BICD2 result in altered interactomes and dynein hyperactivity Open
Cytoplasmic dynein-1 (dynein) is responsible for the transport of most cellular cargo towards the minus end of microtubules. Dynein activation requires the multi-subunit dynactin complex and an activating cargo adaptor. The adaptors serve …
View article: Reviewer #1 (Public review): Dominant spinal muscular atrophy linked mutations in the cargo binding domain of BICD2 result in altered interactomes and dynein hyperactivity
Reviewer #1 (Public review): Dominant spinal muscular atrophy linked mutations in the cargo binding domain of BICD2 result in altered interactomes and dynein hyperactivity Open
View article: Dominant spinal muscular atrophy linked mutations in the cargo binding domain of BICD2 result in altered interactomes and dynein hyperactivity
Dominant spinal muscular atrophy linked mutations in the cargo binding domain of BICD2 result in altered interactomes and dynein hyperactivity Open
Cytoplasmic dynein-1 (dynein) is responsible for the transport of most cellular cargo towards the minus end of microtubules. Dynein activation requires the multi-subunit dynactin complex and an activating cargo adaptor. The adaptors serve …