Phosphoenolpyruvate carboxylase
View article: A Tale of Two Sugars: Trehalose 6-Phosphate and Sucrose
A Tale of Two Sugars: Trehalose 6-Phosphate and Sucrose Open
Trehalose 6-phosphate (Tre6P), the intermediate of trehalose biosynthesis, is an essential signal metabolite in plants, linking growth and development to carbon status. The Suc-Tre6P nexus model postulates that Tre6P is both a signal and n…
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Nitrogen supply influences photosynthesis establishment along the sugarcane leaf Open
Nitrogen (N) is a major component of the photosynthetic apparatus and is widely used as a fertilizer in crops. However, to the best of our knowledge, the dynamic of photosynthesis establishment due to differential N supply in the bioenergy…
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Molecular basis of autotrophic vs mixotrophic growth in Chlorella sorokiniana Open
In this work, we investigated the molecular basis of autotrophic vs. mixotrophic growth of Chlorella sorokiniana , one of the most productive microalgae species with high potential to produce biofuels, food and high value compounds. To inc…
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Metabolite pools and carbon flow during C<sub>4</sub>photosynthesis in maize:<sup>13</sup>CO<sub>2</sub>labeling kinetics and cell type fractionation Open
Worldwide efforts to engineer C4 photosynthesis into C3 crops require a deep understanding of how this complex pathway operates. CO2 is incorporated into four-carbon metabolites in the mesophyll, which move to the bundle sheath where they …
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Installation of C<sub>4</sub> photosynthetic pathway enzymes in rice using a single construct Open
Summary Introduction of a C 4 photosynthetic mechanism into C 3 crops offers an opportunity to improve photosynthetic efficiency, biomass and yield in addition to potentially improving nitrogen and water use efficiency. To create a two‐cel…
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Regulation of Symbiotic Nitrogen Fixation in Legume Root Nodules Open
In most legume nodules, the di-nitrogen (N2)-fixing rhizobia are present as organelle-like structures inside their root host cells. Many processes operate and interact within the symbiotic relationship between plants and nodules, including…
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Metabolite Profiling of Wheat Seedlings Induced by Chitosan: Revelation of the Enhanced Carbon and Nitrogen Metabolism Open
Chitosan plays an important role in regulating growth and eliciting defense in many plant species. However, the exact metabolic response of plants to chitosan is still not clear. The present study performed an integrative analysis of metab…
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Different Growth and Physiological Responses to Cadmium of the Three Miscanthus Species Open
Miscanthus has been proposed as a promising crop for phytoremediation due to its high biomass yield and remarkable adaptability to different environments. However, little is known about the resistance of Miscanthus spp. to cadmium (Cd). To…
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Stomatal Biology of CAM Plants Open
Recent advances in the stomatal biology of CAM plants are reviewed, and key opportunities for future progress are identified.
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Improved analysis of C<sub>4</sub>and C<sub>3</sub>photosynthesis via refined<i>in vitro</i>assays of their carbon fixation biochemistry Open
Plants operating C3 and C4 photosynthetic pathways exhibit differences in leaf anatomy and photosynthetic carbon fixation biochemistry. Fully understanding this underpinning biochemical variation is requisite to identifying solutions for i…
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Genome-wide expression profiling in leaves and roots of date palm (Phoenix dactylifera L.) exposed to salinity Open
Leaf and root tissues respond differentially to salinity stress and this study has revealed genes and pathways that are associated with responses to elevated NaCl levels and thus may play important roles in salt tolerance providing a found…
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Increased biomass accumulation in maize grown in mixed nitrogen supply is mediated by auxin synthesis Open
The use of mixed nitrate and ammonium as a nitrogen source can improve plant growth. Here, we used metabolomics and transcriptomics to study the underlying mechanisms. Maize plants were grown hydroponically in the presence of three forms o…
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Alternative Crassulacean Acid Metabolism Modes Provide Environment-Specific Water-Saving Benefits in a Leaf Metabolic Model Open
Crassulacean acid metabolism (CAM) evolved in arid environments as a water-saving alternative to C3 photosynthesis. There is great interest in engineering more drought-resistant crops by introducing CAM into C3 plants. However, it is unkno…
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Phosphorylation of Phospho <i>enol</i> pyruvate Carboxylase Is Essential for Maximal and Sustained Dark CO <sub>2</sub> Fixation and Core Circadian Clock Operation in the Obligate Crassulacean Acid Metabolism Species <i>Kalanchoë fedtschenkoi</i> Open
Phosphoenolpyruvate carboxylase (PPC; EC 4.1.1.31) catalyzes primary nocturnal CO2 fixation in Crassulacean acid metabolism (CAM) species. CAM PPC is regulated posttranslationally by a circadian clock-controlled protein kinase called phosp…
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Acclimation of Biochemical and Diffusive Components of Photosynthesis in Rice, Wheat, and Maize to Heat and Water Deficit: Implications for Modeling Photosynthesis Open
The impact of the combined effects of heat stress, increased vapor pressure deficit (VPD) and water deficit on the physiology of major crops needs to be better understood to help identifying the expected negative consequences of climate ch…
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<i>Kalanchoë</i> PPC1 Is Essential for Crassulacean Acid Metabolism and the Regulation of Core Circadian Clock and Guard Cell Signaling Genes Open
Unlike C3 plants, Crassulacean acid metabolism (CAM) plants fix CO2 in the dark using phosphoenolpyruvate carboxylase (PPC; EC 4.1.1.31). PPC combines phosphoenolpyruvate with CO2 (as HCO3 -), forming oxaloacetate. The oxaloacetate is conv…
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Shade compromises the photosynthetic efficiency of NADP-ME less than that of PEP-CK and NAD-ME C4 grasses Open
The high energy cost and apparently low plasticity of C4 photosynthesis compared with C3 photosynthesis may limit the productivity and distribution of C4 plants in low light (LL) environments. C4 photosynthesis evolved numerous times, but …
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Improved sugar-free succinate production by Synechocystis sp. PCC 6803 following identification of the limiting steps in glycogen catabolism Open
Succinate produced by microorganisms can replace currently used petroleum-based succinate but typically requires mono- or poly-saccharides as a feedstock. The cyanobacterium Synechocystis sp. PCC6803 can produce organic acids such as succi…
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Phosphorus uptake, partitioning and redistribution during grain filling in rice Open
The results demonstrate that P uptake from the soil during grain filling is a critical contributor to the P content in grains in irrigated rice. The P tracer study suggests that the mechanism of P loading into grains involves little direct…
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Construction and modular implementation of the THETA cycle for synthetic CO2 fixation Open
Synthetic biology offers the opportunity to build solutions for improved capture and conversion of carbon dioxide (CO 2 ) that outcompete those evolved by nature. Here we demonstrate the design and construction of a new-to-nature CO 2 -fix…
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Efficient Regulation of CO2 Assimilation Enables Greater Resilience to High Temperature and Drought in Maize Open
Increasing temperatures and extended drought episodes are among the major constraints affecting food production. Maize has a relatively high temperature optimum for photosynthesis compared to C 3 crops, however, the response of this import…
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The complex character of photosynthesis in cucumber fruit Open
The surface area of a mature green cucumber (Cucumis sativa L.) fruit is comparable with that of a functional leaf, but the characteristics of fruit photosynthesis and its contribution to growth are poorly understood. Here, the photosynthe…
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Melatonin Enhances Drought Tolerance by Regulating Leaf Stomatal Behavior, Carbon and Nitrogen Metabolism, and Related Gene Expression in Maize Plants Open
It is commonly known that exogenously applied melatonin can alleviate the impact of drought stress, but the mechanism used by melatonin to regulate stomatal behavior and carbon (C) and nitrogen (N) metabolism to increase drought resistance…
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Transcriptome analysis reveals global regulation in response to CO2 supplementation in oleaginous microalga Coccomyxa subellipsoidea C-169 Open
Global and collaborative regulation was employed by C-169 to assimilate more carbon and maintain carbon/nitrogen balance upon elevated CO2, which provide abundant carbon skeleton and affluent metabolic energy to sustain rapid growth and li…
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Low Salinity Improves Photosynthetic Performance in Panicum antidotale Under Drought Stress Open
Salinity and drought are two often simultaneously occurring abiotic stresses that limit the production of food crops worldwide. This study aimed to distinguish between the separate and combined impacts of drought and salinity on the plant …
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Effects of reduced carbonic anhydrase activity on CO<sub>2</sub>assimilation rates in<i>Setaria viridis</i>: a transgenic analysis Open
In C4 species, the major β-carbonic anhydrase (β-CA) localized in the mesophyll cytosol catalyses the hydration of CO2 to HCO3-, which phosphoenolpyruvate carboxylase uses in the first step of C4 photosynthesis. To address the role of CA i…
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Laying the Foundation for Crassulacean Acid Metabolism (CAM) Biodesign: Expression of the C4 Metabolism Cycle Genes of CAM in Arabidopsis Open
Crassulacean acid metabolism (CAM) is a specialized mode of photosynthesis that exploits a temporal CO2 pump with nocturnal CO2 uptake and concentration to reduce photorespiration, improve water-use efficiency (WUE), and optimize the adapt…
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Expression of the Maize Dof1 Transcription Factor in Wheat and Sorghum Open
Nitrogen is essential for plant growth and development. Improving the ability of plants to acquire and assimilate nitrogen more efficiently is a key agronomic parameter that will augment sustainability in agriculture. A transcription facto…
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Metabolic engineering of Ustilago trichophora TZ1 for improved malic acid production Open
Ustilago trichophora RK089 has been found recently as a good natural malic acid producer from glycerol. This strain has previously undergone adaptive laboratory evolution for enhanced substrate uptake rate resulting in the strain U. tricho…
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High temperature during soybean seed development differentially alters lipid and protein metabolism Open
High temperatures during seed development can affect the seed yield and quality in many crops. Here, we analyzed how high temperature alters the main seed storage compounds (lipid and protein) in soybean. At five days after R5 stage (initi…