Echinocyte
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Measuring cell surface area and deformability of individual human red blood cells over blood storage using quantitative phase imaging Open
The functionality and viability of stored human red blood cells (RBCs) is an important clinical issue in transfusions. To systematically investigate changes in stored whole blood, the hematological properties of individual RBCs were quanti…
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Rapid clearance of storage-induced microerythrocytes alters transfusion recovery Open
Permanent availability of red blood cells (RBCs) for transfusion depends on refrigerated storage, during which morphologically altered RBCs accumulate. Among these, a subpopulation of small RBCs, comprising type III echinocytes, spheroechi…
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A coarse-grained red blood cell membrane model to study stomatocyte-discocyte-echinocyte morphologies Open
An improved red blood cell (RBC) membrane model is developed based on the bilayer coupling model (BCM) to accurately predict the complete sequence of stomatocyte-discocyte-echinocyte (SDE) transformation of a RBC. The coarse-grained (CG)-R…
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MD/DPD Multiscale Framework for Predicting Morphology and Stresses of Red Blood Cells in Health and Disease Open
Healthy red blood cells (RBCs) have remarkable deformability, squeezing through narrow capillaries as small as 3 microns in diameter without any damage. However, in many hematological disorders the spectrin network and lipid bilayer of dis…
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Red blood cells ageing markers: a multi-parametric analysis. Open
Various types of lesions accumulated at different chemical and cellular levels during storage, which could impact their in vivo recovery after transfusion. A marked effect was observed after four weeks of storage, which corroborates recent…
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Washing stored red blood cells in an albumin solution improves their morphologic and hemorheologic properties Open
BACKGROUND Prolonged storage of red blood cells (RBCs) leads to storage lesions, which may impair clinical outcomes after transfusion. A hallmark of storage lesions is progressive echinocytic shape transformation, which can be partially re…
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Time Dependent Assessment of Morphological Changes: Leukodepleted Packed Red Blood Cells Stored in SAGM Open
Usually packed red blood cells (pRBCs) require specific conditions in storage procedures to ensure the maximum shelf life of up to 42 days in 2–6°C. However, molecular and biochemical consequences can affect the stored blood cells; these c…
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Donor‐dependent aging of young and old red blood cell subpopulations: Metabolic and functional heterogeneity Open
Background Characteristics of red blood cells (RBCs) are influenced by donor variability. This study assessed quality and metabolomic variables of RBC subpopulations of varied biologic age in red blood cell concentrates (RCCs) from male an…
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Shape matters: the effect of red blood cell shape on perfusion of an artificial microvascular network Open
BACKGROUND The shape of human red blood cells (RBCs) deteriorates progressively throughout hypothermic storage, with echinocytosis being the most prevalent pathway of this morphologic lesion. As a result, each unit of stored blood contains…
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An Automated Method for Counting Red Blood Cells using Image Processing Open
In healthcare, blood testing is observed to be as one of the most significant medical examination tests. In pathology labs, different types of blood cells are counted to diagnose the diseases patient. By counting RBCs (Red Blood Cells) in …
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The Influence of Oxidative Stress and Natural Antioxidants on Morphometric Parameters of Red Blood Cells, the Hemoglobin Oxygen Binding Capacity, and the Activity of Antioxidant Enzymes Open
Using a wide range of different physical and chemical methods, it was found that the oxidative stress caused by addition of hydrogen peroxide to the incubation medium has a significant effect on the conformation of haematoporphyrin, influe…
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Three-dimensional counting of morphologically normal human red blood cells via digital holographic microscopy Open
Counting morphologically normal cells in human red blood cells (RBCs) is extremely beneficial in the health care field. We propose a three-dimensional (3-D) classification method of automatically determining the morphologically normal RBCs…
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Maintenance and regulation of asymmetric phospholipid distribution in human erythrocyte membranes: implications for erythrocyte functions Open
Purpose of review The article summarizes new insights into the molecular mechanisms for the maintenance and regulation of the asymmetric distribution of phospholipids in human erythrocyte membranes. We focus on phosphatidylserine, which is…
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Molecular mechanism of action of chlorogenic acid on erythrocyte and lipid membranes Open
The high antioxidant capacity of chlorogenic acid (CGA) in respect to biological systems is commonly known, though the molecular mechanism underlying that activity is not known. The aim of the study was to determine that mechanism at the m…
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<i>In vivo</i>photoacoustic microscopy of human cuticle microvasculature with single-cell resolution Open
As a window on the microcirculation, human cuticle capillaries provide rich information about the microvasculature, such as its morphology, density, dimensions, or even blood flow speed. Many imaging technologies have been employed to imag…
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Evaluation of the CellaVision Advanced RBC Application for Detecting Red Blood Cell Morphological Abnormalities Open
The Advanced RBC Application of CellaVision DM9600 is a valuable screening tool for detecting RBC morphological abnormalities.
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Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy Open
The present study describes utilization of two photon excitation fluorescence (2PE) microscopy for visualization of the hemoglobin in human and porcine erythrocytes and their empty membranes (i.e., ghosts). High-quality, label- and fixatio…
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An In Vitro Study of the Effect of Cytotoxic Triorganotin Dimethylaminophenylazobenzoate Complexes on Red Blood Cells Open
Interactions of tributyltin (TBTA) and triphenyltin (TPhTA) 2-[4 (dimethylamino)phenylazo]benzoates, showing promising cytostatic activity against tumor cells, with erythrocytes and with erythrocyte membranes and model lipid membranes have…
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Automatic Classification of Red Blood Cell Morphology Based on Quantitative Phase Imaging Open
Classification of the morphology of red blood cells (RBCs) plays an extremely important role in evaluating the quality of long‐term stored blood, as RBC storage lesions such as transformation of discocytes to echinocytes and then to sphero…
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Effect of silver nanoparticles on some blood parameters in rats Open
The present study aims are investigating the effects of oral administration of AgNPs on some blood the parameters to show potential of toxicity of AgNPs as tool therapy and diagnosis. In this study, 20 healthy male and female rats were use…
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Influence of Standard Laboratory Procedures on Measures of Erythrocyte Damage Open
The ability to characterize the mechanical properties of erythrocytes is important in clinical and research contexts: to diagnose and monitor hematologic disorders, as well as to optimize the design of cardiovascular implants and blood cir…
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Influence of Pre-Storage Irradiation on the Oxidative Stress Markers, Membrane Integrity, Size and Shape of the Cold Stored Red Blood Cells Open
Background: To investigate the extent of oxidative damage and changes in morphology of manually isolated red blood cells (RBCs) from whole blood, cold stored (up to 20 days) in polystyrene tubes and subjected to pre-storage irradiation (50…
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Modelling of Red Blood Cell Morphological and Deformability Changes during In-Vitro Storage Open
Storage lesion is a critical issue facing transfusion treatments, and it adversely affects the quality and viability of stored red blood cells (RBCs). RBC deformability is a key indicator of cell health. Deformability measurements of each …
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Internalization of nano- and micro-plastics in human erythrocytes leads to oxidative stress and estrogen receptor-mediated cellular responses Open
Plastic material versatility has resulted in a substantial increase in its use in several sectors of our everyday lives. Consequently, concern regarding human exposure to nano-plastics (NPs) and micro-plastics (MPs) has recently increased.…
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RedTell: an AI tool for interpretable analysis of red blood cell morphology Open
Introduction: Hematologists analyze microscopic images of red blood cells to study their morphology and functionality, detect disorders and search for drugs. However, accurate analysis of a large number of red blood cells needs automated c…
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Automated three-dimensional morphology-based clustering of human erythrocytes with regular shapes: stomatocytes, discocytes, and echinocytes Open
We present unsupervised clustering methods for automatic grouping of human red blood cells (RBCs) extracted from RBC quantitative phase images obtained by digital holographic microscopy into three RBC clusters with regular shapes, includin…
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Effective cell membrane tension protects red blood cells against malaria invasion Open
A critical step in how malaria parasites invade red blood cells (RBCs) is the wrapping of the membrane around the egg-shaped merozoites. Recent experiments have revealed that RBCs can be protected from malaria invasion by high membrane ten…
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Hemolysis Pathways during Storage of Erythrocytes and Inter-Donor Variability in Erythrocyte Morphology Open
Background: Red blood cells (RBCs) stored for transfusions can lyse over the course of the storage period. The lysis is traditionally assumed to occur via the formation of spiculated echinocyte forms, so that cells that appear smoother are…
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Storage Primes Erythrocytes for Necroptosis and Clearance Open
Storage-induced sensitization to RBC necroptosis and clearance is important as it may be relevant to hemolytic transfusion reactions.
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Understanding the Behaviour of Human Cell Types under Simulated Microgravity Conditions: The Case of Erythrocytes Open
Erythrocytes are highly specialized cells in human body, and their main function is to ensure the gas exchanges, O2 and CO2, within the body. The exposure to microgravity environment leads to several health risks such as those affecting re…