Microscopy ≈ MicroscopyMicroscopy
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Expansion microscopy Open
Overcoming the limits of the microscope The resolution of a light microscope is limited. Physicists have long since worked out what these limits are and which parameters determine the spatial resolution. Many groups have nevertheless made …
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Atomic structure of sensitive battery materials and interfaces revealed by cryo–electron microscopy Open
Frozen in time The electrochemical processes occurring in a battery are highly dynamic. To understand the complexities of the charge and discharge cycles, you need to be able to watch the processes in situ or to freeze the battery rapidly …
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Suite2p: beyond 10,000 neurons with standard two-photon microscopy Open
Two-photon microscopy of calcium-dependent sensors has enabled unprecedented recordings from vast populations of neurons. While the sensors and microscopes have matured over several generations of development, computational methods to proc…
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Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes Open
Superresolution imaging in sharper focus An optical microscope cannot distinguish objects separated by less than half the wavelength of light. Superresolution techniques have broken this “diffraction limit” and provided exciting new insigh…
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Fibril structure of amyloid-β(1–42) by cryo–electron microscopy Open
Elucidating pathological fibril structure Amyloid-β (Aβ) is a key pathological contributor to Alzheimer's disease. Gremer et al. used cryoelectron microscopy data to build a high-quality de novo atomic model of Aβ fibrils (see the Perspect…
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Nucleus segmentation across imaging experiments: the 2018 Data Science Bowl Open
Segmenting the nuclei of cells in microscopy images is often the first step in the quantitative analysis of imaging data for biological and biomedical applications. Many bioimage analysis tools can segment nuclei in images but need to be s…
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Atomic force microscopy-based mechanobiology Open
Mechanobiology emerges at the crossroads of medicine, biology, biophysics and engineering and describes how the responses of proteins, cells, tissues and organs to mechanical cues contribute to development, differentiation, physiology and …
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Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications Open
FLIM is advantageous for probing molecular environments of fluorophores to inform on fluorophore behavior that cannot be elucidated with intensity measurements alone. Development of FLIM technologies, analysis, and applications will furthe…
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Visualizing and discovering cellular structures with super-resolution microscopy Open
Super-resolution microscopy has overcome a long-held resolution barrier—the diffraction limit—in light microscopy and enabled visualization of previously invisible molecular details in biological systems. Since their conception, super-reso…
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ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging Open
Imaging techniques for visualizing and analyzing precise morphology and gene expression patterns are essential for understanding biological processes during development in all organisms. With the aid of chemical screening, we developed a c…
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Deep learning microscopy Open
We demonstrate that a deep neural network can significantly improve optical microscopy, enhancing its spatial resolution over a large field of view and depth of field. After its training, the only input to this network is an image acquired…
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Deep-STORM: super-resolution single-molecule microscopy by deep learning Open
We present an ultra-fast, precise, parameter-free method, which we term\nDeep-STORM, for obtaining super-resolution images from stochastically-blinking\nemitters, such as fluorescent molecules used for localization microscopy.\nDeep-STORM …
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Long-range hot-carrier transport in hybrid perovskites visualized by ultrafast microscopy Open
Extraction of photoexcited charge carriers transported up to 600 nanometers in CH 3 NH 3 PbI 3 could boost solar cell efficiency.
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Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method Open
Single-cell analysis reveals aspects of cellular physiology not evident from population-based studies, particularly in the case of highly multiplexed methods such as mass cytometry (CyTOF) able to correlate the levels of multiple signallin…
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Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms Open
Continuing the resolution revolution The living cell contains dynamic, spatially complex subassemblies that are sensitive to external perturbations. To minimize such perturbations, cells should be imaged in their native multicellular envir…
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Super-Resolution Structured Illumination Microscopy Open
Super-resolved structured illumination microscopy (SR-SIM) is among the most rapidly growing fluorescence microscopy techniques that can surpass the optical diffraction limit. The strength of SR-SIM is that it can be readily applied to sam…
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Robust Nucleus/Cell Detection and Segmentation in Digital Pathology and Microscopy Images: A Comprehensive Review Open
Digital pathology and microscopy image analysis is widely used for comprehensive studies of cell morphology or tissue structure. Manual assessment is labor intensive and prone to interobserver variations. Computer-aided methods, which can …
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Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking Open
Single-molecule super-resolution fluorescence microscopy and single-particle tracking are two imaging modalities that illuminate the properties of cells and materials on spatial scales down to tens of nanometers or with dynamical informati…
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Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy Open
Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and cellular structures at the nanometer scale. Employing these techniques in living cells has opened up the possibility to study dynamic processes with …
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3D intensity and phase imaging from light field measurements in an LED array microscope Open
Realizing high resolution across large volumes is challenging for 3D imaging techniques with high-speed acquisition. Here, we describe a new method for 3D intensity and phase recovery from 4D light field measurements, achieving enhanced re…
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2.2 Å resolution cryo-EM structure of β-galactosidase in complex with a cell-permeant inhibitor Open
Pushing the limits of electron microscopy Recent advances in cryo–electron microscopy (cryo-EM) allow structures of large macromolecules to be determined at near-atomic resolution. So far, though, resolutions approaching 2 Å, where feature…
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smiFISH and FISH-quant – a flexible single RNA detection approach with super-resolution capability Open
Single molecule FISH (smFISH) allows studying transcription and RNA localization by imaging individual mRNAs in single cells. We present smiFISH (single molecule inexpensive FISH), an easy to use and flexible RNA visualization and quantifi…
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An automated approach for microplastics analysis using focal plane array (FPA) FTIR microscopy and image analysis Open
We present an automated approach to reduce the time demand currently needed for data analyses. We have developed a novel analysis pipeline, followed by image analysis with Python and Simple ITK image processing modules.
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Classifying and segmenting microscopy images with deep multiple instance learning Open
Motivation : High-content screening (HCS) technologies have enabled large scale imaging experiments for studying cell biology and for drug screening. These systems produce hundreds of thousands of microscopy images per day and their utilit…
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Oufti: an integrated software package for high‐accuracy, high‐throughput quantitative microscopy analysis Open
Summary With the realization that bacteria display phenotypic variability among cells and exhibit complex subcellular organization critical for cellular function and behavior, microscopy has re‐emerged as a primary tool in bacterial resear…
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Deep Learning of Atomically Resolved Scanning Transmission Electron Microscopy Images: Chemical Identification and Tracking Local Transformations Open
Recent advances in scanning transmission electron and scanning probe microscopies have opened exciting opportunities in probing the materials structural parameters and various functional properties in real space with angstrom-level precisi…
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Discrimination Between Normal and Cancerous Cells Using AFM Open
Currently, biomechanics of living cells is in the focus of interest due to noticeable capability of such techniques like atomic force microscopy (AFM) to probe cellular properties at the single cell level directly on living cells. The rese…
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Cortical column and whole-brain imaging with molecular contrast and nanoscale resolution Open
Combining expansion and the lattice light sheet Optical and electron microscopy have made tremendous inroads into understanding the complexity of the brain. Gao et al. introduce an approach for high-resolution tracing of neurons, their sub…
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Ultrasound Localization Microscopy and Super-Resolution: A State of the Art Open
Because it drives the compromise between resolution and penetration, the diffraction limit has long represented an unreachable summit to conquer in ultrasound imaging. Within a few years after the introduction of optical localization micro…
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Ultrabright and Fluorogenic Probes for Multicolor Imaging and Tracking of Lipid Droplets in Cells and Tissues Open
Lipid droplets (LDs) are intracellular lipid-rich organelles that regulate the storage of neutral lipids and were recently found to be involved in many physiological processes, metabolic disorders, and diseases including obesity, diabetes,…