Interference microscopy
View article
Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics Open
When imaging through tissue, the optical inhomogeneities of the sample generate aberrations that can prevent effective Stimulated Emission Depletion (STED) imaging. This is particularly problematic for 3D-enhanced STED. We present here an …
View article
Fundamental aspects of resolution and precision in vertical scanning white-light interferometry Open
We discuss the height and lateral resolution that can be achieved in vertical scanning white-light interferometry (SWLI). With respect to interferometric height resolution, phase-shifting interferometry (PSI) is assumed to provide the high…
View article
Stable and simple quantitative phase-contrast imaging by Fresnel biprism Open
Digital holographic (DH) microscopy has grown into a powerful nondestructive technique for the real-time study of living cells including dynamic membrane changes and cell fluctuations in nanometer and sub-nanometer scales. The conventional…
View article
Epi-illumination gradient light interference microscopy for imaging opaque structures Open
Multiple scattering and absorption limit the depth at which biological tissues can be imaged with light. In thick unlabeled specimens, multiple scattering randomizes the phase of the field and absorption attenuates light that travels long …
View article
Line-field confocal time-domain optical coherence tomography with dynamic focusing Open
A time-domain optical coherence tomography technique is introduced for high-resolution B-scan imaging in real-time. The technique is based on a two-beam interference microscope with line illumination and line detection using a broadband sp…
View article
Spatial light interference microscopy: principle and applications to biomedicine Open
In this paper, we review spatial light interference microscopy (SLIM), a common-path, phase-shifting interferometer, built onto a phase-contrast microscope, with white-light illumination. As one of the most sensitive quantitative phase ima…
View article
Tutorial: Common path self-referencing digital holographic microscopy Open
Quantitative phase imaging of cells provides important morphological information about them, leading to their characterization, comparison, and identification. The interference principle when applied to microscopy provides high-contrast qu…
View article
Single-exposure quantitative phase imaging in color-coded LED microscopy Open
We demonstrate single-shot quantitative phase imaging (QPI) in a platform of color-coded LED microscopy (cLEDscope). The light source in a conventional microscope is replaced by a circular LED pattern that is trisected into subregions with…
View article
Controlling the wettability of polycarbonate substrates by producing hierarchical structures using Direct Laser Interference Patterning Open
New strategies for the fabrication of surface structures using Direct Laser Interference Patterning are presented in this work, aiming to fabricate hierarchical structures with selective wetting properties. Polycarbonate sheets have been s…
View article
Label-free colorectal cancer screening using deep learning and spatial light interference microscopy (SLIM) Open
Current pathology workflow involves staining of thin tissue slices, which otherwise would be transparent, followed by manual investigation under the microscope by a trained pathologist. While the hematoxylin and eosin (H&E) stain is well-e…
View article
Development of a general model for direct laser interference patterning of polymers Open
This study investigates the general mechanism of Direct Laser Interference Patterning (DLIP) involved in the structuring process of polymer materials. An empirical model is developed taking into account experimental observations of DLIP-tr…
View article
Lens aberration compensation in interference microscopy Open
Emergence of products that feature functional surfaces with complex geometries, such as freeform optics in consumer electronics and augmented reality and virtual reality, requires high-accuracy non-contact surface measurement. However, lar…
View article
Scattering and three-dimensional imaging in surface topography measuring interference microscopy Open
Surface topography measuring interference microscopy is a three-dimensional (3D) imaging technique that provides quantitative analysis of industrial and biomedical specimens. Many different instrument modalities and configurations exist, b…
View article
High-Resolution Quantitative Phase Imaging of Plasmonic Metasurfaces with Sensitivity down to a Single Nanoantenna Open
Optical metasurfaces have emerged as a new generation of building blocks for multifunctional optics. Design and realization of metasurface elements place ever-increasing demands on accurate assessment of phase alterations introduced by com…
View article
LED-based interference-reflection microscopy combined with optical tweezers for quantitative three-dimensional microtubule imaging Open
Optical tweezers combined with various microscopy techniques are a versatile tool for single-molecule force spectroscopy. However, some combinations may compromise measurements. Here, we combined optical tweezers with total-internal-reflec…
View article
Structured illumination microscopy for dual-modality 3D sub-diffraction\n resolution fluorescence and refractive-index reconstruction Open
Though structured illumination (SI) microscopy is a popular imaging technique\nconventionally associated with fluorescent super-resolution, recent works have\nsuggested its applicability towards sub-diffraction coherent imaging with\nquant…
View article
Biomimetic Surface Structuring Using Laser Based Interferometric Methods Open
This review investigates the capabilities of laser-based interferometric methods for producing structures with multiple-scaled surface features imitating natural examples. Firstly, laser interference lithography is used to produce hierarch…
View article
Multifunctional Hierarchical Surface Structures by Femtosecond Laser Processing Open
Hierarchical surface structures were fabricated on fused silica by using a fs-laser with a pulse duration τ = 300 fs and a wavelength λ = 512 nm. The resulting surface structures were characterized by scanning electron microscopy, atomic f…
View article
Microsphere-assisted phase-shifting profilometry Open
In the present work, we have investigated the combination of a superresolution microsphere-assisted 2D imaging technique with low-coherence phase-shifting interference microscopy. The imaging performance of this technique is studied by num…
View article
Physical modeling of interference enhanced imaging and characterization of single nanoparticles Open
Interferometric imaging schemes have gained significant interest due to their superior sensitivity over imaging techniques that are solely based on scattered signal. In this study, we outline the theoretical foundations of imaging and char…
View article
Signal modeling in low coherence interference microscopy on example of rectangular grating Open
Besides the illumination wavelength also the numerical aperture (NA) of a microscope objective affects the fringe spacing in interference microscopy. Therefore, at high NA values an effective wavelength should be obtained by calibration. A…
View article
Modeling of interference microscopy beyond the linear regime Open
Coherence scanning interferometry (CSI), a type of interference microscopy, has found broad applications in advanced manufacturing industry providing high-accuracy surface topography measurement. Enhancement of the metrological capability …
View article
Review of interferometric spectroscopy of scattered light for the quantification of subdiffractional structure of biomaterials Open
Optical microscopy is the staple technique in the examination of microscale material structure in basic science and applied research. Of particular importance to biology and medical research is the visualization and analysis of the weakly …
View article
Compensated Microsphere-Assisted Interference Microscopy Open
International audience
View article
Single-shot incoherent color digital holographic microscopy system with static polarization-sensitive optical elements Open
We propose a digital holographic microscope for the single-shot multicolor three-dimensional (3D) image sensing of specimens illuminated by spatially and temporally incoherent white light. The proposed microscope exploits self-interference…
View article
Coherence scanning and phase imaging optical interference microscopy at the lateral resolution limit Open
To get physical insight into the 3D transfer characteristics of interference microscopy at high numerical apertures we study reflecting rectangular grating structures. In general, the height obtained from phase information seems to be redu…
View article
Wide field of view common-path lateral-shearing digital holographic interference microscope Open
Quantitative three-dimensional (3-D) imaging of living cells provides important information about the cell morphology and its time variation. Off-axis, digital holographic interference microscopy is an ideal tool for 3-D imaging, parameter…
View article
Wavefront Microscopy Using Quadriwave Lateral Shearing Interferometry: From Bioimaging to Nanophotonics Open
International audience
View article
Snapshot multi-wavelength interference microscope Open
A snapshot multi-wavelength interference microscope is proposed for high-speed measurement of large vertical range discontinuous microstructures and surface roughness. A polarization CMOS camera with a linear micro-polarizer array and Baye…
View article
Single-shot two-frame π-shifted spatially multiplexed interference phase microscopy Open
Single-shot, two-frame, π-shifted spatially multiplexed interference microscopy (π-SMIM) is presented as an improvement to previous SMIM implementations, introducing a versatile, robust, fast, and accurate method for cumbersome, noisy, and…