Autofluorescence
View article: Fluorophores in the near-infrared region
Fluorophores in the near-infrared region Open
Fluorescence imaging in the near-infrared (NIR) and short-wave infrared (SW-IR) ranges has emerged as a promising area, providing wide-ranging possibilities for use in both biology and medicine. This spectral range is advantageous for biol…
View article: High content 3D Imaging by dual-view oblique plane microscopy
High content 3D Imaging by dual-view oblique plane microscopy Open
Oblique plane microscopy (OPM) is a form of light-sheet fluorescence microscopy (LSFM) employing a single microscope objective at the sample for both fluorescence excitation and detection. Dual-view OPM (dOPM) is an optically folded form o…
View article: Imaging flow cytometry enables label-free cell sorting of morphological variants from populations of the unculturable bacterium <i>Pasteuria ramosa</i>
Imaging flow cytometry enables label-free cell sorting of morphological variants from populations of the unculturable bacterium <i>Pasteuria ramosa</i> Open
Bacterial populations often display remarkable morphological heterogeneity. Fluorescence-activated cell sorting (FACS) is an important tool for understanding this diversity. FACs allows researchers to obtain pure samples of each morphologi…
View article: An Infrared Energy Device for the Treatment of Facial Skin Aging
An Infrared Energy Device for the Treatment of Facial Skin Aging Open
Objectives: To evaluate the cellular and clinical responses to facial treatment using a wide-spectrum, long-pulse infrared medical device. Methods: Thirty patients with facial skin laxity and photodamage underwent a single treatment sessio…
View article: CLEAR-AF: Improved Autofluorescence Subtraction for Multiplexed Tissue Imaging via Polar Transformation and Gaussian Mixture Models
CLEAR-AF: Improved Autofluorescence Subtraction for Multiplexed Tissue Imaging via Polar Transformation and Gaussian Mixture Models Open
Cyclic Multiplexed ImmunoFluorescence (cMIF) can profile dozens to hundreds of proteins at subcellular resolution, offering unprecedented insights into tissue architecture in health and disease. However, measured signals are affected by ti…
View article: Autofluorescence alone is not enough: a combined strategy for accurate identification of parathyroid tissue in thyroidectomy specimens
Autofluorescence alone is not enough: a combined strategy for accurate identification of parathyroid tissue in thyroidectomy specimens Open
Importance: Inadvertent excision of parathyroid glands (PTGs) during thyroidectomy remains a common and significant surgical complication, potentially leading to hypoparathyroidism. While near-infrared autofluorescence (NIRAF) imaging enha…
View article: Diagnostic Performance of Ring Aperture Retro Mode Imaging for Detecting Pigment Migration in Age-Related Macular Degeneration (AMD)
Diagnostic Performance of Ring Aperture Retro Mode Imaging for Detecting Pigment Migration in Age-Related Macular Degeneration (AMD) Open
Background/Objectives: Pigment migration is a key biomarker of progression in age-related macular degeneration (AMD). This study assessed the diagnostic performance of ring aperture Retro mode (RAR) imaging for detecting pigment migration …
View article: Compatibility of fast tissue clearing with targeted fluorescence imaging and pathology analysis using clinical head and neck cancer biopsies
Compatibility of fast tissue clearing with targeted fluorescence imaging and pathology analysis using clinical head and neck cancer biopsies Open
Complete tumor removal is crucial for successful surgical oncological outcomes. Targeted fluorescence imaging facilitates differentiation between healthy and cancerous tissue, enabling intraoperative margin assessment. However, its depth s…
View article: Altered dimerization of certain riboflavin transporter 2 mutants: a possible source of UPR, altered calcium signalling and mitochondrial derangements in RTD2
Altered dimerization of certain riboflavin transporter 2 mutants: a possible source of UPR, altered calcium signalling and mitochondrial derangements in RTD2 Open
Riboflavin transporter deficiency Type 2 (RTD2, OMIM #614707), formerly known as Brown-Vialetto-Van Laere Syndrome 2 (BVVLS 2), is a rare autosomal recessive neurodegenerative disorder caused by biallelic variants in the SLC52A2 gene, enco…
View article: Cellular responses of the green parasitic microalga Coccomyxa parasitica to osmotic stress
Cellular responses of the green parasitic microalga Coccomyxa parasitica to osmotic stress Open
Osmotic stress profoundly affects cellular physiology and behavior in microalgae, yet its impact on parasitic species remains poorly characterized. In this study, we investigated the response of the parasitic microalga Coccomyxa parasitica…
View article: Soybean single-seed respiration evaluation through spectral imaging
Soybean single-seed respiration evaluation through spectral imaging Open
Seed respiration is a key metabolic process linked to physiological status. Q2 respiration analysis enables detailed profiling of individual seeds, and combined with multispectral imaging, allows to explore seed-to-seed relationships betwe…
View article: Method for Lignin Analysis in Wood by Fluorescence Microscopy
Method for Lignin Analysis in Wood by Fluorescence Microscopy Open
Native Amazonian species present high anatomical variation which is reflected in their lignin content. This study tested different lignin fluorescence treatments and fluorescence intensity processing methods in native Amazonian woods. Vali…
View article: Deep Learning Virtual Staining Dataset: Breast, Mouse, and Organoids
Deep Learning Virtual Staining Dataset: Breast, Mouse, and Organoids Open
This dataset contains comprehensive image data for training and evaluating deep learning models dedicated to virtual staining of histopathological images. The goal is to generate H&E-like pathology images from autofluorescence (AF) or mult…
View article: Deep Learning Virtual Staining Dataset: Breast, Mouse, and Organoids
Deep Learning Virtual Staining Dataset: Breast, Mouse, and Organoids Open
This dataset contains comprehensive image data for training and evaluating deep learning models dedicated to virtual staining of histopathological images. The goal is to generate H&E-like pathology images from autofluorescence (AF) or mult…
View article: Denoising of fluorescence lifetime imaging data via principal component analysis
Denoising of fluorescence lifetime imaging data via principal component analysis Open
Fluorescence Lifetime Imaging Microscopy (FLIM) quantifies autofluorescence lifetime to assess cellular metabolism, therapeutic efficacy, and disease progression. These dynamic and heterogeneous processes complicate signal analysis. Fit-fr…
View article: Parathyroid autofluorescence: A conditionally cost-effective tool to reduce postoperative hypocalcemia after total thyroidectomy
Parathyroid autofluorescence: A conditionally cost-effective tool to reduce postoperative hypocalcemia after total thyroidectomy Open
View article: Plant cell wall enzymatic hydrolysis: Predicting yield dynamics from autofluorescence and morphological temporal changes
Plant cell wall enzymatic hydrolysis: Predicting yield dynamics from autofluorescence and morphological temporal changes Open
Enzymatic hydrolysis of plant cell walls into fermentable sugars is a critical step in biotechnological conversion, yet efficiency is limited by cell wall recalcitrance. Predicting conversion yields of cell wall-derived sugars during hydro…
View article: Protocol for autofluorescence removal in microglia by photobleaching in free-floating immunofluorescent staining of mouse brain tissue
Protocol for autofluorescence removal in microglia by photobleaching in free-floating immunofluorescent staining of mouse brain tissue Open
View article: Supplemental Analysis Pipeline from Wide-Field Optical Redox Imaging with Leading-Edge Detection Enables Assessment of Treatment Response and Heterogeneity in Patient-Derived Cancer Organoids
Supplemental Analysis Pipeline from Wide-Field Optical Redox Imaging with Leading-Edge Detection Enables Assessment of Treatment Response and Heterogeneity in Patient-Derived Cancer Organoids Open
Supplemental Analysis pipeline contains the CellProfiler pipeline used to generate leading-edge data reported in the manuscript. Dependency: CellProfiler v4.0.7 Inputs: Widefield Autofluorescence images of patient derived cancer organoids …
View article: Data from Wide-Field Optical Redox Imaging with Leading-Edge Detection Enables Assessment of Treatment Response and Heterogeneity in Patient-Derived Cancer Organoids
Data from Wide-Field Optical Redox Imaging with Leading-Edge Detection Enables Assessment of Treatment Response and Heterogeneity in Patient-Derived Cancer Organoids Open
Patient-derived cancer organoids (PDCO) are a valuable model to recapitulate human disease in culture, with important implications for drug development. However, current methods for rapidly and reproducibly assessing PDCOs are limited. Lab…
View article: Improving detection accuracy of heterogeneity in biological tissues through the combination of modulation-demodulation frame accumulation techniques and enhanced vgg16
Improving detection accuracy of heterogeneity in biological tissues through the combination of modulation-demodulation frame accumulation techniques and enhanced vgg16 Open
Light source has obvious absorption and scattering effects during the transmission process of biological tissues, making it difficult to identify heterogeneities in multi-spectral images. This paper achieves a gradual improvement in the cl…
View article: HAMscope: a snapshot Hyperspectral Autofluorescence Miniscope for real-time molecular imaging
HAMscope: a snapshot Hyperspectral Autofluorescence Miniscope for real-time molecular imaging Open
We introduce HAMscope, a compact, snapshot hyperspectral autofluorescence miniscope that enables real-time, label-free molecular imaging in a wide range of biological systems. By integrating a thin polymer diffuser into a widefield minisco…
View article: HAMscope: a snapshot Hyperspectral Autofluorescence Miniscope for real-time molecular imaging
HAMscope: a snapshot Hyperspectral Autofluorescence Miniscope for real-time molecular imaging Open
We introduce HAMscope, a compact, snapshot hyperspectral autofluorescence miniscope that enables real-time, label-free molecular imaging in a wide range of biological systems. By integrating a thin polymer diffuser into a widefield minisco…
View article: Acridine Orange dye for long-term staining and live imaging of cnidarian development and regeneration
Acridine Orange dye for long-term staining and live imaging of cnidarian development and regeneration Open
As the sister phylum to Bilateria, Cnidaria, holds a phylogenetic position that is crucial in evolutionary studies of animal development. However, the analysis of cnidarian species, as well as other emerging models, is limited by the scarc…
View article: Genetically encoding stimulated Raman-scattering probes for cell imaging using infrared fluorescent proteins
Genetically encoding stimulated Raman-scattering probes for cell imaging using infrared fluorescent proteins Open
Stimulated Raman scattering (SRS) microscopy offers great potential to surpass fluorescent-based approaches, owing to the sharp linewidth of Raman vibrations amenable to super-multiplex cell imaging, but currently lacks one crucial compone…
View article: Genetically encoding stimulated Raman-scattering probes for cell imaging using infrared fluorescent proteins
Genetically encoding stimulated Raman-scattering probes for cell imaging using infrared fluorescent proteins Open
Stimulated Raman scattering (SRS) microscopy offers great potential to surpass fluorescent-based approaches, owing to the sharp linewidth of Raman vibrations amenable to super-multiplex cell imaging, but currently lacks one crucial compone…
View article: Light sheet microscopy and spectroscopy of whispering gallery mode microlaser particles
Light sheet microscopy and spectroscopy of whispering gallery mode microlaser particles Open
Whispering gallery mode microlasers are a recently developed sensing tool which can be used to make localised measurements of refractive index changes in a biological tissue through examination of their emission spectrum. This allows the d…
View article: Flow cytometry dataset for OMIP publication_Petti
Flow cytometry dataset for OMIP publication_Petti Open
OMIP-XX: A 27-color spectral flow cytometry panel integrating intrinsic cell autofluorescence for the comprehensive characterization of mouse epithelial tumor immune microenvironment and draining lymph nodes. Luciana Petti1, Hyame Djebbour…
View article: Autofluorescence of Renal Tissue and Its Impact on Fluorescence‐Guided Lithotripsy
Autofluorescence of Renal Tissue and Its Impact on Fluorescence‐Guided Lithotripsy Open
When illuminated with green light, tissue shows negligible autofluorescence in comparison to urinary stones. In automatically controlled lithotripsy, this property is utilized to prevent the laser from being triggered if the fiber is mispo…
View article: Flow cytometry dataset for OMIP publication_Petti
Flow cytometry dataset for OMIP publication_Petti Open
OMIP-XX: A 27-color spectral flow cytometry panel integrating intrinsic cell autofluorescence for the comprehensive characterization of mouse epithelial tumor immune microenvironment and draining lymph nodes. Luciana Petti1, Hyame Djebbour…