Supercritical fluid chromatography
View article: Solvent Supercritical Fluid Technologies to Extract Bioactive Compounds from Natural Sources: A Review
Solvent Supercritical Fluid Technologies to Extract Bioactive Compounds from Natural Sources: A Review Open
Supercritical fluid technologies offer a propitious method for drug discovery from natural sources. Such methods require relatively short processing times, produce extracts with little or no organic co-solvent, and are able to extract bioa…
View article
Supercritical fluids in separation and purification: A review Open
The properties of the supercritical fluid near the critical point can be changed by varying the pressure and temperature, allowing selective and faster extraction. Supercritical fluids exhibit liquid-like density and gas – like viscosity &…
View article
Optimizing separations in online comprehensive two‐dimensional liquid chromatography Open
Online comprehensive two‐dimensional liquid chromatography has become an attractive option for the analysis of complex nonvolatile samples found in various fields (e.g. environmental studies, food, life, and polymer sciences). Two‐dimensio…
View article
Mixed-mode chromatography in pharmaceutical and biopharmaceutical applications Open
Mixed-mode chromatography (MMC) is a fast growing area in recent years, thanks to the new generation of mixed-mode stationary phases and better understanding of multimode interactions. MMC has superior applications in the separation of com…
View article
The Application of Supercritical Fluid Extraction in Phenolic Compounds Isolation from Natural Plant Materials Open
The separation of phenolic compounds by supercritical fluid extraction has been widely studied throughout the last two decades. This is evidenced by a number of publications and articles. Supercritical fluid extraction (SFE) has become thu…
View article
Recent trends in chiral supercritical fluid chromatography Open
This paper presents a review of the recent literature covering separations of enantiomers achieved with supercritical fluid chromatography (SFC) or subcritical fluid chromatography. First of all, the recent progress in SFC technology are e…
View article
A Review: Uses of Gas Chromatography-Mass Spectrometry (GC-MS) Technique for Analysis of Bioactive Natural Compounds of Some Plants Open
Chromatography is the term used to describe a separation technique in which a mobile phase carrying a mixture is caused to move in contact with a selectively absorbent stationary phase. It also plays a fundamental role as an analytical tec…
View article
RPLC-HILIC and SFC with Mass Spectrometry: Polarity-Extended Organic Molecule Screening in Environmental (Water) Samples Open
Trace organic compounds are important in environmental analysis because they impact water quality and introduce potential (eco)toxicological effects. Current analytical methods mostly rely on gas chromatography (GC) or reversed-phase liqui…
View article
Lipidomics by Supercritical Fluid Chromatography Open
This review enlightens the role of supercritical fluid chromatography (SFC) in the field of lipid analysis. SFC has been popular in the late 1980s and 1990s before almost disappearing due to the commercial success of liquid chromatography …
View article
High-Performance Liquid Chromatography (HPLC): A review Open
Today HPLC is widely applied for separations and purifications in a variety of areas including pharmaceuticals, biotechnology, environmental, polymer and food industries. It is accomplished by injection of a small amount of liquid sample i…
View article
Coupling Front-End Separations, Ion Mobility Spectrometry, and Mass Spectrometry For Enhanced Multidimensional Biological and Environmental Analyses Open
Ion mobility spectrometry (IMS) is a widely used analytical technique for rapid molecular separations in the gas phase. Though IMS alone is useful, its coupling with mass spectrometry (MS) and front-end separations is extremely beneficial …
View article
Molecularly Imprinted Polymers for the Identification and Separation of Chiral Drugs and Biomolecules Open
Molecularly imprinting polymers (MIPs) have been extensively applied in chromatography for the separation of chiral drugs. In this review, we mainly summarize recent developments of various MIPs used as chiral stationary phases (CSPs) in h…
View article
Chiral Separations in Preparative Scale: A Medicinal Chemistry Point of View Open
Enantiomeric separation is a key step in the development of a new chiral drug. Preparative liquid chromatography (LC) continues to be the technique of choice either during the drug discovery process, to achieve a few milligrams, or to a sc…
View article
Recent advances in liquid and gas chromatography methodology for extending coverage of the metabolome Open
The metabolome is the complete complement of metabolites (small organic biomolecules). In order to comprehensively understand the effect of stimuli on a biological system, it is important to detect as many of the metabolites within that sy…
View article
Supercritical Extraction Techniques for Obtaining Biologically Active Substances from a Variety of Plant Byproducts Open
Supercritical fluid extraction (SFE) techniques have garnered significant attention as green and sustainable methods for obtaining biologically active substances from a diverse array of plant byproducts. This paper comprehensively reviews …
View article
Supercritical fluid extraction as a suitable technology to recover bioactive compounds from flowers Open
Flowers are the most fragile plant organ that contains a wide range of variable compounds. Due to their nutritional, pharmacological and aromatic properties, they have been used in several products, from food to the pharmaceutical, and tex…
View article
Pushing the speed limit in enantioselective supercritical fluid chromatography Open
Chromatographic enantioseparations on the order of a few seconds can be achieved by supercritical fluid chromatography using short columns packed with chiral stationary phases. The evolution of ‘world record’ speeds for the chromatographic…
View article
<i>Cannabis</i> through the looking glass: chemo- and enantio-selective separation of phytocannabinoids by enantioselective ultra high performance supercritical fluid chromatography Open
An extreme ee was measured for Δ9-THC in medicinal marijuana, by applying the ICCA method under eUHPSFC, using sub-2 μm Whelk-O1 columns.
View article
Metamorphosis of supercritical fluid chromatography: A viable tool for the analysis of polar compounds? Open
Recent developments in supercritical fluid chromatography (SFC) have highlighted the applicability of this technique for the analysis of highly polar compounds. The combination of polar stationary phases and CO2-based mobile phases with an…
View article
Recent Achievements and Future Challenges in Supercritical Fluid Chromatography for the Enantioselective Separation of Chiral Pharmaceuticals Open
During the last years, supercritical fluid chromatography (SFC) has attracted a continuously growing number of users. Thanks to the introduction of state-of-the-art equipment, this technique has allowed to run three-to-five times faster se…
View article
Preparative Scale Resolution of Enantiomers Enables Accelerated Drug Discovery and Development Open
The provision of pure enantiomers is of increasing importance not only for the pharmaceutical industry but also for agro-chemistry and biotechnology. In drug discovery and development, the enantiomers of a chiral drug depict unique chemica…
View article
Co-Solvent Selection for Supercritical Fluid Extraction (SFE) of Phenolic Compounds from Labisia pumila Open
A preliminary study was conducted to study the effects of different types and concentrations of co-solvents based on yield, composition and antioxidants capacity of extract prior to optimization studies of supercritical fluid extraction (S…
View article
Environmentally Friendly Procedure Based on Supercritical Fluid Chromatography and Tandem Mass Spectrometry Molecular Networking for the Discovery of Potent Antiviral Compounds from <i>Euphorbia semiperfoliata</i> Open
A supercritical fluid chromatography-based targeted purification procedure using tandem mass spectrometry and molecular networking was developed to analyze, annotate, and isolate secondary metabolites from complex plant extract mixture. Th…
View article
Analysis of Enantiomers in Products of Food Interest Open
The separation of enantiomers has been started in the past and continues to be a topic of great interest in various fields of research, mainly because these compounds could be involved in biological processes such as, for example, those re…
View article
Supercritical Fluid Chromatography – Theoretical Background and Applications on Natural Products Open
The use of supercritical fluid chromatography for natural product analysis as well as underlying theoretical mechanisms and instrumental requirements are summarized in this review. A short introduction focusing on the historical developmen…
View article
Emerging Developments in Separation Techniques and Analysis of Chiral Pharmaceuticals Open
Chiral separation, the process of isolating enantiomers from a racemic mixture, holds paramount importance in diverse scientific disciplines. Using chiral separation methods like chromatography and electrophoresis, enantiomers can be isola…
View article
Recent developments in preparative-scale supercritical fluid- and liquid chromatography for chiral separations Open
International audience
View article
Expanding the potential of chiral chromatography for high-throughput screening of large compound libraries by means of sub–2μm Whelk-O 1 stationary phase in supercritical fluid conditions Open
With the aim of exploring the potential of ultra-fast chiral chromatography for high-throughput analysis, the new sub-2 micron Whelk-O 1 chiral stationary phase (CSP) has been employed in supercritical fluid conditions to screen 129 racema…
View article
Use of on‐line supercritical fluid extraction‐supercritical fluid chromatography/tandem mass spectrometry to analyze disease biomarkers in dried serum spots compared with serum analysis using liquid chromatography/tandem mass spectrometry Open
Rationale The analytical stability and throughput of biomarker assays based on dried serum spots (DSS) are strongly dependent on the extraction process and determination method. In the present study, an on‐line system based on supercritica…
View article
Quantitative analysis of vitamin D and its main metabolites in human milk by supercritical fluid chromatography coupled to tandem mass spectrometry Open
A novel method to quantitate vitamin D and its main metabolites (vitamin D3, vitamin D2, and their 25-hydroxy metabolites) in breast milk by supercritical fluid chromatography has been developed and fully validated. A small volume of sampl…