Plasmid
View article: Figure S5 from TRIM50 Inhibits Gastric Cancer Progression by Regulating the Ubiquitination and Nuclear Translocation of JUP
Figure S5 from TRIM50 Inhibits Gastric Cancer Progression by Regulating the Ubiquitination and Nuclear Translocation of JUP Open
Figure S5: 293T cells were transfected with Myc-TRIM50 plasmid, Flag-JUP plasmid and HA-Ub plasmid, whole-cell lysates were subjected to SDS denaturation and immunoprecipitation with JUP antibody and immunoblot analysis with the indicated …
View article: Lactiplantibacillus koreensis sp. nov. and Lactiplantibacillus kimchii sp. nov., isolated from kimchi, a traditional Korean fermented food
Lactiplantibacillus koreensis sp. nov. and Lactiplantibacillus kimchii sp. nov., isolated from kimchi, a traditional Korean fermented food Open
Two Gram-stain-positive, facultatively anaerobic, rod-shaped, and non-motile lactic acid bacterial strains, designated as strains CBA3605T and CBA3606T, were isolated from kimchi, a traditional Korean fermented food. Both strains were oxid…
View article: Dual Transmission Dynamics of Carbapenem Resistance in Pediatric Oncology: Plasmid-Mediated and Clonal Spread of blaNDM-5 Enterobacterales
Dual Transmission Dynamics of Carbapenem Resistance in Pediatric Oncology: Plasmid-Mediated and Clonal Spread of blaNDM-5 Enterobacterales Open
CRE bloodstream infections in pediatric oncology patients are driven by both clonal expansion and plasmid-mediated dissemination, with plasmids playing a dominant role, especially in E. coli. These findings highlight the limitations of str…
View article: Construction and Validation of an E. coli Whole-Cell Biosensor Based on CadC and GFP for Heavy Metal Detection
Construction and Validation of an E. coli Whole-Cell Biosensor Based on CadC and GFP for Heavy Metal Detection Open
Heavy metal contamination is a serious environmental and public health concern that demands rapid and reliable detection methods. Whole-cell biosensors offer a cost-effective and sensitive approach for real-time monitoring. In this study, …
View article: Novel insights into the molecular mechanisms of LGMDD2: role of TNPO3 in experimental cell and zebrafish models
Novel insights into the molecular mechanisms of LGMDD2: role of TNPO3 in experimental cell and zebrafish models Open
Transportin 3 (TNPO3) is a nuclear carrier for serine/arginine-rich proteins which are essential for mRNA splicing and metabolism. Mutations in the TNPO3 gene result in a protein with an extended C-terminal domain, leading to the onset of …
View article: Comparison of the Presence of Heavy Metal Resistance Genes in Salmonella enterica and Their Association with Antibiotic Resistance
Comparison of the Presence of Heavy Metal Resistance Genes in Salmonella enterica and Their Association with Antibiotic Resistance Open
Metals are widely used in animal feed for their growth-stimulating and antimicrobial effects; yet, there is potential concern that their use can promote antimicrobial resistance through co-selection. However, the prevalence of these metal …
View article: Test TLE3 ChIP-seq SCP1
Test TLE3 ChIP-seq SCP1 Open
Anti-FLAG ChIP-seq (RPBPM normalized) for ParB1-3xFLAG in Streptomyces coelicolor A3(2) plasmid SCP1. Files included: - TLE3_SCP1.bw: BigWig coverage track scp1_fixed.fa: Reference genome sequence SCP1_genes_only.gff3: Gene annotations Dat…
View article: Figure 1 from EML4-ALK Variant 3 Promotes Mitotic Errors and Spindle Assembly Checkpoint Deficiency Leading to Increased Microtubule Poison Sensitivity
Figure 1 from EML4-ALK Variant 3 Promotes Mitotic Errors and Spindle Assembly Checkpoint Deficiency Leading to Increased Microtubule Poison Sensitivity Open
Expression of EML4-ALK V3 increases microtubule stability in mitosis. A, H3122 and H2228 cells were incubated on ice for 15 minutes before being fixed and stained with α-tubulin (red) and CENP-A (green) antibodies. DNA was stained w…
View article: Structure of the conjugation surface exclusion protein TraT
Structure of the conjugation surface exclusion protein TraT Open
Conjugal transfer of plasmids between bacteria is a major route for the spread of antimicrobial resistance. Many conjugative plasmids encode exclusion systems that inhibit redundant conjugation. In incompatibility group F (IncF) plasmids s…
View article: Reviewer #2 (Public review): SMC5/6-mediated plasmid silencing is directed by SIMC1–SLF2 and antagonized by the SV40 large T antigen
Reviewer #2 (Public review): SMC5/6-mediated plasmid silencing is directed by SIMC1–SLF2 and antagonized by the SV40 large T antigen Open
View article: SMC5/6-mediated plasmid silencing is directed by SIMC1–SLF2 and antagonized by the SV40 large T antigen
SMC5/6-mediated plasmid silencing is directed by SIMC1–SLF2 and antagonized by the SV40 large T antigen Open
SMC5/6 is unique among the Structural Maintenance of Chromosomes (SMC) complexes in its ability to repress transcription from extrachromosomal circular DNA (ecDNA), including viral genomes and plasmids. Previously, we showed that human SMC…
View article: Generation of a HiBiT-expressing recombinant rat hepacivirus supporting both in vivo and in vitro infection
Generation of a HiBiT-expressing recombinant rat hepacivirus supporting both in vivo and in vitro infection Open
The lack of immuno-competent animal models of hepatitis C virus (HCV) infection has been an obstacle to vaccine development and research on immune responses. Hepacivirus ratti (Norway rat hepacivirus-1: NRHV1) is a virus closely related to…
View article: Reviewer #1 (Public review): SMC5/6-mediated plasmid silencing is directed by SIMC1–SLF2 and antagonized by the SV40 large T antigen
Reviewer #1 (Public review): SMC5/6-mediated plasmid silencing is directed by SIMC1–SLF2 and antagonized by the SV40 large T antigen Open
View article: Correction to “Chondroitin Sulphate Coated DNA‐Nanoplexes Enhances Transfection Efficiency by Controlling Plasmid Release from Endosomes: A New Insight into Modulating Non‐Viral Gene Transfection”
Correction to “Chondroitin Sulphate Coated DNA‐Nanoplexes Enhances Transfection Efficiency by Controlling Plasmid Release from Endosomes: A New Insight into Modulating Non‐Viral Gene Transfection” Open
View article: eLife Assessment: SMC5/6-mediated plasmid silencing is directed by SIMC1–SLF2 and antagonized by the SV40 large T antigen
eLife Assessment: SMC5/6-mediated plasmid silencing is directed by SIMC1–SLF2 and antagonized by the SV40 large T antigen Open
View article: Author response: SMC5/6-mediated plasmid silencing is directed by SIMC1–SLF2 and antagonized by the SV40 large T antigen
Author response: SMC5/6-mediated plasmid silencing is directed by SIMC1–SLF2 and antagonized by the SV40 large T antigen Open
View article: Human gut strains of <i>Desulfovibrio piger</i> exhibit spontaneous induction of multiple prophages
Human gut strains of <i>Desulfovibrio piger</i> exhibit spontaneous induction of multiple prophages Open
Sulfate-reducing bacterium Desulfovibrio piger is a common member of the human gastrointestinal microbiome, associated with inflammatory conditions but also prevalent in healthy individuals. This suggests that lifestyle factors may shape i…
View article: Reviewer #3 (Public review): SMC5/6-mediated plasmid silencing is directed by SIMC1–SLF2 and antagonized by the SV40 large T antigen
Reviewer #3 (Public review): SMC5/6-mediated plasmid silencing is directed by SIMC1–SLF2 and antagonized by the SV40 large T antigen Open
View article: Figure 5 from EML4-ALK Variant 3 Promotes Mitotic Errors and Spindle Assembly Checkpoint Deficiency Leading to Increased Microtubule Poison Sensitivity
Figure 5 from EML4-ALK Variant 3 Promotes Mitotic Errors and Spindle Assembly Checkpoint Deficiency Leading to Increased Microtubule Poison Sensitivity Open
SAC deficiencies in cells expressing EML4-ALK V3 are dependent on ALK activity. A, Beas-2B parental cells were either mock transfected or transfected with plasmids encoding YFP alone, YFP-EML4-ALK V3 WT (wild-type) or YFP-EML4-ALK V…
View article: Cytokinin-mediated trichome initiation in <i>Nicotiana benthamiana</i> upon <i>Agrobacterium tumefaciens</i> infiltration
Cytokinin-mediated trichome initiation in <i>Nicotiana benthamiana</i> upon <i>Agrobacterium tumefaciens</i> infiltration Open
Infiltration of Agrobacterium tumefaciens in leaves of Nicotiana benthamiana has become a standard method for transient expression in plants. Like many Solanaceae species, N. benthamiana develops on its leaf surface capitate glandular tric…
View article: Detection and Preliminary Genomic Characterization of Poultry-Derived Salmonella enterica from Southern Kazakhstan
Detection and Preliminary Genomic Characterization of Poultry-Derived Salmonella enterica from Southern Kazakhstan Open
Background/Objectives: Salmonella enterica is a major cause of foodborne infection globally, with poultry acting as an important reservoir. However, data from Central Asia remain limited. This study provides preliminary phenotypic and geno…
View article: Global geographic and genomic epidemiology analysis of carbapenem-resistant <i>Escherichia coli</i> carrying <i>bla</i> <sub>NDM-9</sub>
Global geographic and genomic epidemiology analysis of carbapenem-resistant <i>Escherichia coli</i> carrying <i>bla</i> <sub>NDM-9</sub> Open
Carbapenem-resistant Escherichia coli (CREC), particularly strains producing New Delhi metallo-β-lactamase-9 (NDM-9), pose a growing threat as agents of nosocomial infections. Despite their emergence since 2013, a comprehensive global phyl…
View article: Figure S5 from LAMC2 Regulates Key Transcriptional and Targetable Effectors to Support Pancreatic Cancer Growth
Figure S5 from LAMC2 Regulates Key Transcriptional and Targetable Effectors to Support Pancreatic Cancer Growth Open
Suppl. Figure 5. A. RNA expression of KRAS, AREG, DUSP6 and FOSL1 in PATU8902 and HPAFII cells expressing a TET-inducible KRAS shRNA or a control shRNA. B. Box and whiskers plot showing Mean Fluorescence Intensity (MFI) of Areg, Dusp6, Fos…
View article: kjolley/BIGSdb: Version 1.51.6
kjolley/BIGSdb: Version 1.51.6 Open
This release adds two new analysis plugins and other improvements: New GeneScanner plugin New PlasmidFinder plugin Updates to various plugins to support analysis fields (third-party results stored as JSON within the database) Improved per…
View article: EXPRESSION OF CONJUGATION GENES IS CONTROLLED BY PROCESSIVE ANTITERMINATION AND A NOVEL ZIPPER-TYPE TRANSCRIPTIONAL ATTENUATION MECHANISM
EXPRESSION OF CONJUGATION GENES IS CONTROLLED BY PROCESSIVE ANTITERMINATION AND A NOVEL ZIPPER-TYPE TRANSCRIPTIONAL ATTENUATION MECHANISM Open
Proper expression of genes clustered in operons, particularly large operons, can be complex, often consisting of multiple regulatory switches. The conjugation operon present on the Bacillus subtilis conjugative plasmid pLS20 is over 32 kb …
View article: Prevalent and diverse new plasmid-encoded heavy metal and antimicrobial resistance in Klebsiella strains isolated from hospital wastewater
Prevalent and diverse new plasmid-encoded heavy metal and antimicrobial resistance in Klebsiella strains isolated from hospital wastewater Open
The rise of antibiotic-resistant Klebsiella pneumoniae poses a significant global health threat. Plasmids, as mobile genetic elements, play a critical role in bacterial adaptation by facilitating the spread of resistance genes. To analyze …
View article: Liposome-mediated co-delivery of enzalutamide and PMEPA1 overexpression plasmid suppresses prostate cancer progression by targeting the androgen receptor signaling pathway
Liposome-mediated co-delivery of enzalutamide and PMEPA1 overexpression plasmid suppresses prostate cancer progression by targeting the androgen receptor signaling pathway Open
View article: Genomic characterisation of multidrug-resistant Salmonella enterica serovar Kentucky ST198 isolates from various sources in Algeria, North Africa
Genomic characterisation of multidrug-resistant Salmonella enterica serovar Kentucky ST198 isolates from various sources in Algeria, North Africa Open
Salmonella enterica serovar Kentucky ( S . Kentucky) sequence type (ST) 198 has emerged as a globally disseminated multidrug-resistant (MDR) lineage posing significant public health challenges. The aim of this study was to characterise 125…
View article: Development of two compatible plasmids to assess sRNA-mediated post-transcriptional regulation in Acinetobacter baumannii
Development of two compatible plasmids to assess sRNA-mediated post-transcriptional regulation in Acinetobacter baumannii Open
Post-transcriptional regulation can be mediated by small regulatory RNAs (sRNAs) in bacteria, which can act by base-pairing to a target mRNA. The discovery and mechanistic validation of base-pairing sRNAs in multidrug-resistant Acinetobact…
View article: BarTn7: Optimizing Bacterial Lineage Tracking at Sub-Species Resolution for Population Dynamics in Ecological and Evolutionary Studies
BarTn7: Optimizing Bacterial Lineage Tracking at Sub-Species Resolution for Population Dynamics in Ecological and Evolutionary Studies Open
Communities of bacteria undergo population bottlenecks which are crucial to their population, ecological, and evolutionary dynamics. However, conventional amplicon sequencing cannot distinguish such demographic shifts between very closely …