Thrombin
View article: SARS-CoV-2 Spike Protein Amyloid Fibrils Impair Fibrin Formation and Fibrinolysis
SARS-CoV-2 Spike Protein Amyloid Fibrils Impair Fibrin Formation and Fibrinolysis Open
Long COVID, or postacute sequelae of COVID-19 from SARS-CoV-2 infection, is a persistent debilitating disease affecting multiple systems and organs. Long COVID pathophysiology is a complex and not fully established process. One prevailing …
View article: Detrimental Effect of Plasma From Patients With Severe Aortic Stenosis on Valvular Endothelial Cells: Role of Proinflammatory Cytokines and Factor Xa
Detrimental Effect of Plasma From Patients With Severe Aortic Stenosis on Valvular Endothelial Cells: Role of Proinflammatory Cytokines and Factor Xa Open
Background The role of plasma and its components in the progression of aortic stenosis (AS) remains insufficiently investigated. This study aimed to assess whether plasma from patients with AS induces oxidative stress and contributes to va…
View article: Size‐Dependent Ultrasound Activation of Thrombin Catalytic Activity by Mechano‐Nanoswitches
Size‐Dependent Ultrasound Activation of Thrombin Catalytic Activity by Mechano‐Nanoswitches Open
Integrating nanostructures with mechanochemistry significantly boosts drug loading capabilities and sonomechanical drug activation, outperforming traditional polymer systems. Nevertheless, the influence of nanoparticles’ physicochemical pr…
View article: Transferrin-Functionalized Liposomes Enhance MAPT-ASO Transport Across a 3D Blood–Brain Barrier Microvascular Network Model
Transferrin-Functionalized Liposomes Enhance MAPT-ASO Transport Across a 3D Blood–Brain Barrier Microvascular Network Model Open
Tau pathology is a defining hallmark of Alzheimer’s disease (AD), closely associated with cognitive decline. Antisense oligonucleotides targeting the tau-encoding gene MAPT (MAPT-ASO) have shown promise in clinical trials, but their therap…
View article: Heparin Resistance in Cardiac Surgery with Cardiopulmonary Bypass: Mechanisms, Clinical Implications, and Evidence-Based Management
Heparin Resistance in Cardiac Surgery with Cardiopulmonary Bypass: Mechanisms, Clinical Implications, and Evidence-Based Management Open
Background: Unfractionated heparin (UFH) is the standard anticoagulant during cardiopulmonary bypass (CPB). A clinically relevant subset develops heparin resistance (HR)—failure to reach adequate anticoagulation with usual UFH—raising thro…
View article: Next-generation FVIIIa-mimetic bispecific antibody NXT007: evaluation in preclinical models of hemostasis and thrombosis
Next-generation FVIIIa-mimetic bispecific antibody NXT007: evaluation in preclinical models of hemostasis and thrombosis Open
NXT007 is a next-generation factor (F)VIIIa-mimetic bispecific antibody currently in Phase 1/2 trials. It was developed by optimizing the framework of emicizumab to achieve hemostatic normalization in people with hemophilia A (PwHA). Here,…
View article: Activated protein C promotes human lung cancer progression through the release of tumor extracellular vesicles and transfer of microRNA-200a
Activated protein C promotes human lung cancer progression through the release of tumor extracellular vesicles and transfer of microRNA-200a Open
Blood coagulation and cancer are intricately related. Hypercoagulation associated with cancer leads to aberrant thrombin generation, which contributes to thrombosis. Thrombin also activates anticoagulant protein C and the activated protein…
View article: Production, Purification, and Characterization of a Novel Cysteine-Rich Anticoagulant from the Medicinal Leech and the Functional Role of Its C-Terminal Motif
Production, Purification, and Characterization of a Novel Cysteine-Rich Anticoagulant from the Medicinal Leech and the Functional Role of Its C-Terminal Motif Open
The saliva of the medicinal leech Hirudo medicinalis contains a wide range of biologically active compounds, including multiple anticoagulants. Previously, we identified a novel cysteine-rich anticoagulant protein (CRA) from leech saliva a…
View article: To generate functional anti-protease-activated receptor-4 (PAR4), a G protein-coupled receptor, antibodies through PAR4-mRNA-LNP immunization
To generate functional anti-protease-activated receptor-4 (PAR4), a G protein-coupled receptor, antibodies through PAR4-mRNA-LNP immunization Open
G protein-coupled receptors (GPCRs) are key therapeutic targets for various diseases, such as the thrombin receptor protease-activated receptor 4 (PAR4) involved in thrombotic cardiovascular disorders. However, the structural complexity of…
View article: Effects of Neutrophil Extracellular Traps on Platelet-Driven Blood Clot Contraction
Effects of Neutrophil Extracellular Traps on Platelet-Driven Blood Clot Contraction Open
Immunothrombosis can affect substantially the course and outcomes of severe infections and immune-mediated diseases. While inflammatory thrombi are neutrophil-rich, impact of neutrophils on clot contraction, a key modulator of thrombus sta…
View article: Loss of nuclear myosin 1 causes hemostatic defects and immune dysregulation
Loss of nuclear myosin 1 causes hemostatic defects and immune dysregulation Open
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View article: Role of Rubicon in Platelets: A Promoter of Thrombosis but Not an Autophagy Repressor
Role of Rubicon in Platelets: A Promoter of Thrombosis but Not an Autophagy Repressor Open
Rubicon (RUN domain Beclin-1-interacting and cysteine-rich-containing protein) is a negative regulator of autophagy in nucleated cells; however, its role in platelets is unstudied. Here, we identify an autophagy-independent role for Rubico…
View article: Linking Thrombin to α-Synuclein truncation reveals a molecular bridge between neuroinflammation and Parkinson disease
Linking Thrombin to α-Synuclein truncation reveals a molecular bridge between neuroinflammation and Parkinson disease Open
Proteolytic processing of α-Synuclein (Syn) contributes to the molecular diversity and toxicity of its aggregates in Parkinson disease (PD), yet the proteases responsible for generating disease-relevant truncations remain incompletely defi…
View article: eVLP‐Mediated Cas9 Delivery for Preventing IBMIR in Islet Transplantation
eVLP‐Mediated Cas9 Delivery for Preventing IBMIR in Islet Transplantation Open
Islet transplantation is a promising strategy for effective β‐cell replacement in patients with type 1 diabetes. However, its success is hindered significantly by instant blood‐mediated inflammatory reaction (IBMIR), which leads to rapid g…
View article: Novel anticoagulant proteins identified in the salivary glands of Rhipicephalus sanguineus and their interaction with coagulation factor X
Novel anticoagulant proteins identified in the salivary glands of Rhipicephalus sanguineus and their interaction with coagulation factor X Open
In the salivary glands of the hematophagous arthropod R. sanguineus, a total of 122 protein coding sequences interacting with coagulation factor X (FX) were identified using the yeast two-hybrid (Y2H) technology, including 100 characterize…
View article: Serum peptidomics profiling reveals fibrinogen alpha chain-derived peptides associated with clinical outcome in patients with heart failure
Serum peptidomics profiling reveals fibrinogen alpha chain-derived peptides associated with clinical outcome in patients with heart failure Open
Our findings suggest that the complement and coagulation pathway was associated with HF outcome. A FIBA-derived serum peptides model can serve as an outcome classifier in patients with HF.
View article: From Pandemic to Practice: How COVID-19 Has Reshaped Haemostasis in Cardiac Surgery: A Narrative Review
From Pandemic to Practice: How COVID-19 Has Reshaped Haemostasis in Cardiac Surgery: A Narrative Review Open
The utilisation of cardiopulmonary bypass (CPB) during cardiac surgery is often associated with complex haemostatic perturbations, frequently manifesting as a paradoxical risk of both bleeding and thrombosis. This is postulated to be drive…
View article: Estrogen and obesity synergistically suppress protein S via HIF1α, enhancing thrombosis potential
Estrogen and obesity synergistically suppress protein S via HIF1α, enhancing thrombosis potential Open
Venous thromboembolism (VTE) is a leading cause of morbidity and mortality, with risk heightened in premenopausal women with obesity or use estrogen-based oral contraceptives. When both risk factors are present, the thrombosis risk increas…
View article: P selectin promotes SARS-CoV-2 interactions with platelets and the endothelium
P selectin promotes SARS-CoV-2 interactions with platelets and the endothelium Open
The physiology of SARS-CoV-2 virus/host interactions is not well understood. To better understand host/virus interactions, we performed a CRISPR activation screen to identify host genes that confer resistance to authentic SARS-CoV-2. This …
View article: Dried plasma retains hemostatic function and thermal stability during Arctic military operations
Dried plasma retains hemostatic function and thermal stability during Arctic military operations Open
Background Dried plasma offers a practical alternative for remote damage control resuscitation, providing hemostatic support and volume replacement. The Arctic presents challenges that necessitate the need for blood‐based resuscitation to …
View article: Transitioning of Patients from Direct-Acting Oral Anticoagulant to Heparin: Impact on Laboratory Testing
Transitioning of Patients from Direct-Acting Oral Anticoagulant to Heparin: Impact on Laboratory Testing Open
Direct oral anticoagulants (DOACs), including direct thrombin inhibitors (dabigatran) and direct factor Xa inhibitors (apixaban, rivaroxaban, edoxaban), have transformed anticoagulant management in recent years due to their predictable pha…
View article: Thrombin generation to predict breakthrough bleeding in patients with acquired hemophilia A under emicizumab prophylaxis
Thrombin generation to predict breakthrough bleeding in patients with acquired hemophilia A under emicizumab prophylaxis Open
Acquired hemophilia A (AHA) is a serious bleeding disorder due to neutralizing autoantibodies against factor VIII (FVIII). Emicizumab mimics the activity of FVIIIa restoring thrombin generation. It was shown to protect patients with AHA fr…
View article: Heparinized Dendritic Mesoporous Silica Nanoparticles for the Selective Adsorption and Isolation of Thrombin
Heparinized Dendritic Mesoporous Silica Nanoparticles for the Selective Adsorption and Isolation of Thrombin Open
View article: A Label-Free Fluorescence Polarization for Protein Detection Based on Albumin Nanoparticles
A Label-Free Fluorescence Polarization for Protein Detection Based on Albumin Nanoparticles Open
Human serum albumin (HSA), an endogenous protein, was employed in the synthesis of nanoparticles. The treatment of an HSA solution with ethanol and glutaraldehyde resulted in the formation of human serum albumin nanoparticles (HSA NPs), wh…
View article: Ex vivo effects of low dose delayed release rapamycin on agonist-induced platelet aggregation, activation and procoagulant platelet phenotypes in domestic cats
Ex vivo effects of low dose delayed release rapamycin on agonist-induced platelet aggregation, activation and procoagulant platelet phenotypes in domestic cats Open
Cardiogenic arterial thromboembolism (CATE) is a complication of hypertrophic cardiomyopathy (HCM) with a high mortality rate. Despite anti-platelet drugs use, on-treatment recurrence rate remains high indicating a critical need to discove…
View article: Albumin coatings for counteracting uremic metabolites effects on host responses to biomaterials
Albumin coatings for counteracting uremic metabolites effects on host responses to biomaterials Open
Chronic kidney disease is a progressive condition characterized by a decline in kidney function that is the impetus for an increased retention of uremic metabolites (UMs) in the blood compartment which is correlated with adverse patient ou…
View article: ALAI MUMIYO (SHILAJIT): TRADITIONAL ORIGINS AND EXPERIMENTAL EVIDENCE OF ANTICOAGULANT AND FIBRINOLYTIC ACTIVITY
ALAI MUMIYO (SHILAJIT): TRADITIONAL ORIGINS AND EXPERIMENTAL EVIDENCE OF ANTICOAGULANT AND FIBRINOLYTIC ACTIVITY Open
The effect of Alai mumiyo extract (25 mg/kg) on blood coagulation in dogs was studied. Ten healthy animals received the extract orally, and coagulation parameters were measured at baseline, 30, and 60 minutes. The extract significantly pro…
View article: ALAI MUMIYO (SHILAJIT): TRADITIONAL ORIGINS AND EXPERIMENTAL EVIDENCE OF ANTICOAGULANT AND FIBRINOLYTIC ACTIVITY
ALAI MUMIYO (SHILAJIT): TRADITIONAL ORIGINS AND EXPERIMENTAL EVIDENCE OF ANTICOAGULANT AND FIBRINOLYTIC ACTIVITY Open
The effect of Alai mumiyo extract (25 mg/kg) on blood coagulation in dogs was studied. Ten healthy animals received the extract orally, and coagulation parameters were measured at baseline, 30, and 60 minutes. The extract significantly pro…
View article: Engineering a membrane-independent human prothrombinase through parsimonious mutation of factor Xa.
Engineering a membrane-independent human prothrombinase through parsimonious mutation of factor Xa. Open
View article: 437 A case study of venous thromboembolism in a patient with congenital afibrinogenemia
437 A case study of venous thromboembolism in a patient with congenital afibrinogenemia Open
Introduction/Objective Arterial and venous thromboembolism are known complications of congenital afibrinogenemia; however, management is challenging due to the lack of established guidelines for anticoagulation regimens. Methods/Case Repor…