Takifugu rubripes
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Identification of the sex-determining locus in grass puffer (Takifugu niphobles) provides evidence for sex-chromosome turnover in a subset of Takifugu species Open
There is increasing evidence for frequent turnover in sex chromosomes in vertebrates. Yet experimental systems suitable for tracing the detailed process of turnover are rare. In theory, homologous turnover is possible if the new sex-determ…
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Analysis of the Amyloidogenic Potential of Pufferfish (<i>Takifugu rubripes</i>) Islet Amyloid Polypeptide Highlights the Limitations of Thioflavin-T Assays and the Difficulties in Defining Amyloidogenicity Open
Islet amyloid polypeptide (IAPP, amylin) forms pancreatic amyloid in type-2 diabetes, a process that contributes to the loss of β-cell mass in the disease. IAPP has been found in all higher organisms examined, but not all species form amyl…
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Branchial nitrogen cycle symbionts can remove ammonia in fish gills Open
Summary Knowledge of the mechanisms by which fish excrete their metabolic nitrogenous waste and insights into nitrogen cycling in aquaculture systems is of utmost importance to improve the sustainable commercial production of fish. In fish…
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Including planocerid flatworms in the diet effectively toxifies the pufferfish, Takifugu niphobles Open
Beginning with the larval stages, marine pufferfish such as Takifugu niphobles contain tetrodotoxin (TTX), an extremely potent neurotoxin. Although highly concentrated TTX has been detected in adults and juveniles of these fish, the source…
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Changes in DNA methylation during epigenetic-associated sex reversal under low temperature in Takifugu rubripes Open
DNA methylation has frequently been implicated in sex determination and differentiation in teleost species. In order to detect the DNA methylation patterns established during sexual differentiation in tiger pufferfish T. rubripes, we perfo…
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An evaluation of microalgae as a recombinant protein oral delivery platform for fish using green fluorescent protein (GFP) Open
Recombinant proteins produced by biological systems such as bacteria, yeasts, mammalian and insect cell cultures are widely used for clinical or industrial purposes. Most therapeutic protein drugs require purification, cold chain, and inje…
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Repeated translocation of a supergene underlying rapid sex chromosome turnover in <i>Takifugu</i> pufferfish Open
Significance Although turnover of sex chromosomes is very common in many vertebrate lineages, the transition process is still elusive. We studied the sex-determining region (SDR) of 12 congeneric fish species of Takifugu fish that compose …
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The effects of five different drying methods on the quality of semi-dried Takifugu obscurus fillets Open
This study investigated the effects of sun drying (SD), hot air drying (HAD), cold air drying (CAD), vacuum drying (VD), and vacuum freeze drying (VFD) on the meat quality of semi-dried Takifugu obscurus fillets. The quality changes in the…
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Toxic Takifugu pardalis eggs found in Takifugu niphobles gut: Implications for TTX accumulation in the pufferfish Open
Pufferfish (Takifugu spp.) possess a potent neurotoxin, tetrodotoxin (TTX). TTX has been detected in various organisms including food animals of pufferfish, and TTX-producing bacteria have been isolated from these animals. TTX in marine pu…
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Dietary taurine stimulates the hepatic biosynthesis of both bile acids and cholesterol in the marine teleost, tiger puffer (<i>Takifugu rubripes</i>) Open
Taurine (TAU) plays important roles in the metabolism of bile acids, cholesterol and lipids. However, little relevant information has been available in fish where TAU has been identified as a conditionally essential nutrient. The present s…
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Characterization and evolutionary analysis of tributyltin‐binding protein and pufferfish saxitoxin and tetrodotoxin‐binding protein genes in toxic and nontoxic pufferfishes Open
Understanding the evolutionary mechanisms of toxin accumulation in pufferfishes has been long‐standing problem in toxicology and evolutionary biology. Pufferfish saxitoxin and tetrodotoxin‐binding protein (PSTBP) is involved in the transpo…
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Contrasting Toxin Selectivity between the Marine Pufferfish Takifugu pardalis and the Freshwater Pufferfish Pao suvattii Open
To clarify the differences in toxin selectivity between marine and freshwater pufferfish, we conducted experiments in artificially reared nontoxic specimens of Takifugu pardalis (marine) and Pao suvattii (freshwater) using tetrodotoxin (TT…
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Adaptive evolution of low-salinity tolerance and hypoosmotic regulation in a euryhaline teleost, Takifugu obscurus Open
The mechanism of osmoregulation is crucial for maintaining growth, development, and life activities in teleosts. Takifugu obscurus, the only euryhaline species in the genus Takifugu, is a proper model organism for studying the mechanism of…
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Deep sequencing, profiling and detailed annotation of microRNAs in Takifugu rubripes Open
We elucidated miRNA expression patterns in various organs of T. rubripes. Most miRNA sequences are conserved in vertebrates, indicating that the basic functions of vertebrate miRNAs share a common evolution. Some miRNA species exhibit diff…
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Pufferfish Saxitoxin and Tetrodotoxin Binding Protein (PSTBP) Analogues in the Blood Plasma of the Pufferfish Arothron nigropunctatus, A. hispidus, A. manilensis, and Chelonodon patoca Open
Pufferfish saxitoxin and tetrodotoxin (TTX) binding protein (PSTBP) is a glycoprotein that we previously isolated from the blood plasma of the pufferfish Takifugu pardalis; this protein was also detected in seven species of the genus Takif…
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A Chromosome—Level Genome Assembly of the Spotted Scat (<i>Scatophagus argus</i>) Open
The spotted scat, Scatophagus argus is a member of the family Scatophagidae found in Indo-Pacific coastal waters. It is an emerging commercial aquaculture species, particularly in East and Southeast Asia. In this study, the first chromosom…
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An Accurate Detection Model of Takifugu rubripes Using an Improved YOLO-V7 Network Open
In aquaculture, the accurate recognition of fish underwater has outstanding academic value and economic benefits for scientifically guiding aquaculture production, which assists in the analysis of aquaculture programs and studies of fish b…
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DDEYOLOv9: Network for Detecting and Counting Abnormal Fish Behaviors in Complex Water Environments Open
Accurately detecting and counting abnormal fish behaviors in aquaculture is essential. Timely detection allows farmers to take swift action to protect fish health and prevent economic losses. This paper proposes an enhanced high-precision …
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Beta-Hydroxysteroid Dehydrogenase Genes in Orange-Spotted Grouper (Epinephelus coioides): Genome-Wide Identification and Expression Analysis During Sex Reversal Open
Beta-hydroxysteroid dehydrogenases (β-HSDs) are a group of steroidogenic enzymes that are involved in steroid biosynthesis and metabolism, and play a crucial role in mammalian physiology and development, including sex determination and dif…
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Copper exposure causes alteration in the intestinal microbiota and metabolites in Takifugu rubripes Open
Copper is an environmental pollutant, and copper in aquatic environments mainly comes from soil and water. It enters the environment through atmospheric deposition, sewage discharge, and industrial production, and enters aquatic organisms,…
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The yellowtail (<i>Seriola quinqueradiata</i>) genome and transcriptome atlas of the digestive tract Open
Seriola quinqueradiata (yellowtail) is the most widely farmed and economically important fish in aquaculture in Japan. In this study, we used the genome of haploid yellowtail fish larvae for de novo assembly of whole-genome sequences, and …
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Characterization and Functional Analysis of Two Transmembrane C-Type Lectins in Obscure Puffer (Takifugu obscurus) Open
C-type lectins (CTLs) have received widespread attention in animal immune responses. In the present study, two CTLs (ToCTL1 and ToCTL2) were identified from obscure puffer Takifugu obscurus. The open reading frames of ToCTL1 and ToCTL2 wer…
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Mucosal IgM Antibody with <span>d</span>-Mannose Affinity in Fugu <i>Takifugu rubripes</i> Is Utilized by a Monogenean Parasite <i>Heterobothrium okamotoi</i> for Host Recognition Open
How parasites recognize their definitive hosts is a mystery; however, parasitism is reportedly initiated by recognition of certain molecules on host surfaces. Fish ectoparasites make initial contact with their hosts at body surfaces, such …
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Relevance of Light Spectra to Growth of the Rearing Tiger Puffer Takifugu rubripes Open
In fish, light (photoperiod, intensity and spectra) is main regulator in many physiological actions includinggrowth. We investigate the effect of light spectra on the somatic growth and growth-related gene expression in the rearing tiger p…
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Source diversity of Artemia enrichment boosts goldfish (Carassius auratus) performance, β-carotene content, pigmentation, immune-physiological and transcriptomic responses Open
This study aimed to assess the impact of spirulina and/or canthaxanthin-enriched Artemia on the goldfish ( Carassius auratus ) growth, pigmentation, blood analysis, immunity, intestine and liver histomorphology, and expression of somatolac…
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Toxicity in Takifugu rubripes exposed to acute ammonia: Effects on immune responses, brain neurotransmitter levels, and thyroid endocrine hormones Open
Exposure to ammonia can cause convulsions, coma, and death. In this study, we investigate the effects of ammonia exposure on immunoregulatory and neuroendocrine changes in Takifugu rubripes. Fish were sampled at 0, 12, 24, 48, and 96 h fol…
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Complete genome of a unicellular parasite (Antonospora locustae) and transcriptional interactions with its host locust Open
Microsporidia are a large group of unicellular parasites that infect insects and mammals. The simpler life cycle of microsporidia in insects provides a model system for understanding their evolution and molecular interactions with their ho…
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Transcriptome Analysis Identifies Key Metabolic Changes in the Brain of Takifugu rubripes in Response to Chronic Hypoxia Open
The brain is considered to be an extremely sensitive tissue to hypoxia, and the brain of fish plays an important role in regulating growth and adapting to environmental changes. As an important aquatic organism in northern China, the econo…
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Genetic Dissection of a Precocious Phenotype in Male Tiger Pufferfish (Takifugu rubripes) using Genotyping by Random Amplicon Sequencing, Direct (GRAS-Di) Open
The novel non-targeted PCR-based genotyping system, namely Genotyping by Random Amplicon Sequencing, Direct (GRAS-Di), is characterized by the simplicity in library construction and robustness against DNA degradation and is expected to fac…
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Genomic selection for heterobothriosis resistance concurrent with body size in the tiger pufferfish, Takifugu rubripes Open
Parasite resistance traits in aquaculture species often have moderate heritability, indicating the potential for genetic improvements by selective breeding. However, parasite resistance is often synonymous with an undesirable negative corr…