Molecular binding
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Optical Biosensors for Label-Free Detection of Small Molecules Open
Label-free optical biosensors are an intriguing option for the analyses of many analytes, as they offer several advantages such as high sensitivity, direct and real-time measurement in addition to multiplexing capabilities. However, develo…
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Quantifying Protein–Protein Interactions by Molecular Counting with Mass Photometry Open
Interactions between biomolecules control the processes of life in health and their malfunction in disease, making their characterization and quantification essential. Immobilization‐ and label‐free analytical techniques are desirable beca…
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Propagating and localized surface plasmon resonance sensing — A critical comparison based on measurements and theory Open
With its potential for ultrasensitive, label-free detection of molecular interactions, sensing methods based on the surface plasmon resonance (SPR) effect fully meet the requirements for modern analytical techniques. Already established by…
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Polymer effects modulate binding affinities in disordered proteins Open
Significance The ability to form large interaction networks was one of the driving forces in the evolution of disordered proteins. However, their advantageous flexibility is accompanied by a tremendous solvent sensitivity that does not see…
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Control of Protein Orientation on Gold Nanoparticles Open
Gold nanoparticles (Au NPs) have attracted much attention due to their potential applications in nano-medicine. While numerous studies have quantified biomolecular adsorption to Au NPs in terms of equilibrium binding constants, far less is…
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Molecular electronics sensors on a scalable semiconductor chip: A platform for single-molecule measurement of binding kinetics and enzyme activity Open
For nearly 50 years, the vision of using single molecules in circuits has been seen as providing the ultimate miniaturization of electronic chips. An advanced example of such a molecular electronics chip is presented here, with the importa…
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KnowVolution of the Polymer-Binding Peptide LCI for Improved Polypropylene Binding Open
The functionalization of polymer surfaces by polymer-binding peptides offers tremendous opportunities for directed immobilization of enzymes, bioactive peptides, and antigens. The application of polymer-binding peptides as adhesion promote…
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Real-time monitoring of binding events on a thermostabilized human A2A receptor embedded in a lipid bilayer by surface plasmon resonance Open
Membrane proteins (MPs) are prevalent drug discovery targets involved in many cell processes. Despite their high potential as drug targets, the study of MPs has been hindered by limitations in expression, purification and stabilization in …
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Photonic ring resonance is a versatile platform for performing multiplex immunoassays in real time Open
Photonic ring resonance is a property of light where in certain circumstances specific wavelengths are trapped in a ring resonator. Sensors based on silicon photonic ring resonators function by detecting the interaction between light circu…
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Recent advances in optical biosensing approaches for biomarkers detection Open
A biomarker is an indicator for sensing the human state of health. There has been a wide-scale investigation into using portable analytical platforms for real-time monitoring of biomarkers. Biomolecular recognition, manufacturing simplific…
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Covalent immobilization of molecularly imprinted polymer nanoparticles using an epoxy silane Open
Molecularly imprinted polymers (MIPs) can be used as antibody mimics to develop robust chemical sensors. One challenging problem in using MIPs for sensor development is the lack of reliable conjugation chemistry that allows MIPs to be fixe…
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Plasmonic Nanosensor Array for Multiplexed DNA-based Pathogen Detection Open
In this research we introduce a plasmonic nanoparticle based optical biosensor for monitoring of molecular binding events. The sensor utilizes spotted gold nanoparticle arrays as sensing platform. The nanoparticle spots are functionalized …
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Salt-mediated two-site ligand binding by the cocaine-binding aptamer Open
Multisite ligand binding by proteins is commonly utilized in the regulation of biological systems and exploited in a range of biochemical technologies. Aptamers, although widely utilized in many rationally designed biochemical systems, are…
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Dictating Nanoparticle Assembly via Systems-Level Control of Molecular Multivalency Open
Nanoparticle assembly can be controlled by multivalent binding interactions between surface ligands, indicating that more precise control over these interactions is important to design complex nanoscale architectures. It has been well-esta…
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Real-Time Label-Free Direct Electronic Monitoring of Topoisomerase Enzyme Binding Kinetics on Graphene Open
Monolayer graphene field-effect sensors operating in liquid have been widely deployed for detecting a range of analyte species often under equilibrium conditions. Here we report on the real-time detection of the binding kinetics of the ess…
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Revisiting the Rate-Limiting Step of the ANS–Protein Binding at the Protein Surface and Inside the Hydrophobic Cavity Open
8-Anilino-1-naphthalenesulfonic acid (ANS) is used as a hydrophobic fluorescence probe due to its high intensity in hydrophobic environments, and also as a microenvironment probe because of its unique ability to exhibit peak shift and inte…
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Resolving Non‐Specific and Specific Adhesive Interactions of Catechols at Solid/Liquid Interfaces at the Molecular Scale Open
The adhesive system of mussels evolved into a powerful and adaptive system with affinity to a wide range of surfaces. It is widely known that thereby 3,4‐dihydroxyphenylalanine (Dopa) plays a central role. However underlying binding energi…
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Rational design of molecularly imprinted polymers Open
We present an integrated theory of molecular imprinting and derive a set of general design principles for molecularly imprinted polymers.
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Multivalent weak interactions enhance selectivity of interparticle binding Open
Significance The selectivity of binding of colloidal particles is an important research topic for the field of targeted drug delivery. Extensive theoretical work has shown that high selectivity can be obtained by using multivalent weak int…
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Quantification of Single-Molecule Protein Binding Kinetics in Complex Media with Prism-Coupled Plasmonic Scattering Imaging Open
Measuring molecular binding is critical for understanding molecular-scale biological processes and screening drugs. Label-free detection technologies, such as surface plasmon resonance (SPR), have been developed for analyzing analytes in t…
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PEGylated Artificial Antibodies: Plasmonic Biosensors with Improved Selectivity Open
Molecular imprinting, which involves the formation of artificial recognition elements or cavities with complementary shape and chemical functionality to the target species, is a powerful method to overcome a number of limitations associate…
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Continuous Small-Molecule Monitoring with a Digital Single-Particle Switch Open
The ability to continuously measure concentrations of small molecules is important for biomedical, environmental, and industrial monitoring. However, because of their low molecular mass, it is difficult to quantify concentrations of such m…
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Characterization of human serum albumin's interactions with safranal and crocin using multi-spectroscopic and molecular docking techniques Open
Interaction mechanisms of human serum albumin (HSA) with safranal and crocin were studied using UV-Vis absorption, fluorescence quenching and circular dichroism (CD) spectroscopies as well as molecular docking techniques. Changes in absorb…
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A Versatile Sub-Nanomolar Fluorescent Ligand Enables NanoBRET Binding Studies and Single-Molecule Microscopy at the Histamine H<sub>3</sub> Receptor Open
The histamine H3 receptor (H3R) is considered an attractive drug target for various neurological diseases. We here report the synthesis of UR-NR266, a novel fluorescent H3R ligand. Broad pharmacological characterization revealed UR-NR266 a…
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Dynamic single-molecule sensing by actively tuning binding kinetics for ultrasensitive biomarker detection Open
Significance The detection of low-abundance molecular biomarkers is key to the liquid–biopsy-based disease diagnosis. Existing methods are limited by the affinity and specificity of recognition probes and the mass transportation of analyte…
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Single‐Molecule Binding Assay Using Nanopores and Dimeric NP Conjugates Open
The ability to measure biomarkers, both specifically and selectively at the single‐molecule level in biological fluids, has the potential to transform the diagnosis, monitoring, and therapeutic intervention of diseases. The use of nanopore…
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Selective Detection of Nitroexplosives Using Molecular Recognition within Self-Assembled Plasmonic Nanojunctions Open
We demonstrate that the reproducibility of sensors for nitroaromatics based on surface-enhanced Raman spectroscopy (SERS) can be significantly improved via a hierarchical aqueous self-assembly approach mediated by the multifunctional macro…
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Isothermal Titration Calorimetry: A Biophysical Method to Characterize the Interaction between Label-free Biomolecules in Solution Open
This protocol can be applied to analyze the direct interaction between a soluble protein and a target ligand molecule using Isothermal Titration Calorimetry (ITC, Malvern). ITC allows the biophysical characterization of binding between lab…
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A Multiscale Simulation Approach to Modeling Drug–Protein Binding Kinetics Open
Drug-target binding kinetics has recently emerged as a sometimes critical determinant of in vivo efficacy and toxicity. Its rational optimization to improve potency or reduce side effects of drugs is, however, extremely difficult. Molecula…
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Biosensor platform for parallel surface plasmon-enhanced epifluorescence and surface plasmon resonance detection Open
For the first time, plasmonic diffraction grating is implemented for the combined direct and epifluorescence-based readout of assays in real-time. This plasmonic structure serves for grating-coupled surface plasmon resonance (SPR) monitori…