Brucella abortus
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<i>Brucella abortus</i> Triggers a cGAS-Independent STING Pathway To Induce Host Protection That Involves Guanylate-Binding Proteins and Inflammasome Activation Open
Immunity against microbes depends on recognition of pathogen-associated molecular patterns by innate receptors. Signaling pathways triggered by Brucella abortus DNA involves TLR9, AIM2, and stimulator of IFN genes (STING). In this study, w…
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Bovine brucellosis: prevalence, risk factors, economic cost and control options with particular reference to India- a review Open
Background: Brucellosis is an economically important zoonotic disease with worldwide distribution, with low-income countries being more affected. The disease is endemic in India, a country that house the world’s largest cattle and buffalo …
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Brucella spp. of amphibians comprise genomically diverse motile strains competent for replication in macrophages and survival in mammalian hosts Open
Twenty-one small Gram-negative motile coccobacilli were isolated from 15 systemically diseased African bullfrogs ( Pyxicephalus edulis ), and were initially identified as Ochrobactrum anthropi by standard microbiological identification sys…
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Brucella abortus Induces a Warburg Shift in Host Metabolism That Is Linked to Enhanced Intracellular Survival of the Pathogen Open
Intracellular bacterial pathogens exploit host cell resources to replicate and survive inside the host. Targeting these host systems is one promising approach to developing novel antimicrobials to treat intracellular infections. We show th…
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Transposon Sequencing of Brucella abortus Uncovers Essential Genes for Growth <i>In Vitro</i> and Inside Macrophages Open
Brucella abortus is a class III zoonotic bacterial pathogen able to survive and replicate inside host cells, including macrophages. Here we report a multidimensional transposon sequencing analysis to identify genes essential for Brucella a…
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Brucella Downregulates Tumor Necrosis Factor-α to Promote Intracellular Survival via Omp25 Regulation of Different MicroRNAs in Porcine and Murine Macrophages Open
Brucella spp. impedes the production of pro-inflammatory cytokines by its outer membrane protein Omp25 in order to promote survival and immune evasion. However, how Omp25 regulates tumor necrosis factor (TNF-α) expression in different mamm…
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ONE Health Approach to Address Zoonotic Brucellosis: A Spatiotemporal Associations Study Between Animals and Humans Open
Background: Brucellosis is one of the most significant zoonosis over the world, threatening both veterinary and human public health. However, few studies were focused on nationwide animal brucellosis and made association with human brucell…
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Control of animal brucellosis: The Malaysian experience Open
Brucellosis is a zoonotic disease characterized by reproductive failure in animals and undulent fever in humans. In cattle, it is caused by Brucella abortus while in goats by Brucella melitensis, the main cause of brucellosis in humans. Br…
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Bovine Brucellosis: Epidemiology, Public Health Implications, and Status of Brucellosis in Ethiopia Open
Dereje Tulu Ethiopia Institute of Agricultural Research, Tepi Agricultural Research Center, Tepi, EthiopiaCorrespondence: Dereje TuluEthiopia Institute of Agricultural Research, Tepi Agricultural Research Center, PO Box 34, Tepi, EthiopiaE…
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Lateral flow biosensor combined with loop-mediated isothermal amplification for simple, rapid, sensitive, and reliable detection of <em>Brucella</em> spp Open
Brucella species is responsible for brucellosis in human and animals, which is still of public health, veterinarian, and economic concern in many regions of the world. Here, a novel molecular diagnosis assay, termed loop-mediated isotherma…
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Interleukin 6 Promotes<i>Brucella abortus</i>Clearance by Controlling Bactericidal Activity of Macrophages and CD8<sup>+</sup>T Cell Differentiation Open
To date, the implications of interleukin 6 (IL-6) for immune responses in the context of Brucella infection are still unknown. In the present study, we found that Brucella abortus infection induced marked production of IL-6 in mice that wa…
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The sero-prevalence of brucellosis in cattle and their herders in Bahr el Ghazal region, South Sudan Open
We report a high prevalence of anti-Brucella antibodies in cattle and their herders in Bahr el Ghazal, indicating an enzootic status in the cattle population being an important source of infection for humans. This represents a genuine publ…
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Molecular identification of <i>Brucella</i> species and biovars associated with animal and human infection in Iran. Open
Brucellosis is a costly contagious disease of human, domestic and wild animals. It is a serious health problem in Iran causing significant economic losses therefore, control approaches to prevent its spread are of great importance. In Iran…
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Improved antibacterial function of Rifampicin-loaded solid lipid nanoparticles on <i>Brucella abortus</i> Open
The objective of this study was to assess the antibacterial activity of Rifampicin-loaded solid lipid nanoparticles on Brucella abortus 544. Rifampicin-loaded solid lipid nanoparticles were prepared by a modified microemulsion/sonication m…
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Isolation of Brucella abortus and Brucella melitensis from Seronegative Cows is a Serious Impediment in Brucellosis Control Open
Brucellosis is a zoonosis occurring worldwide, with economic and public health impacts. Its diagnosis remains a challenge in endemic countries and basically relies on serology. The present study was carried out on two dairy cattle farms al…
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Brucella abortus Infection of Placental Trophoblasts Triggers Endoplasmic Reticulum Stress-Mediated Cell Death and Fetal Loss via Type IV Secretion System-Dependent Activation of CHOP Open
Brucella abortus infects the placenta of pregnant cows, where it replicates to high levels and triggers abortion of the calf. The aborted material is highly infectious and transmits infection to both cows and humans, but very little is kno…
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Brucella-positive raw milk cheese sold on the inner European market: A public health threat due to illegal import? Open
Travel and migration are the major drivers of human brucellosis in Western Europe. The infection is usually transmitted through the consumption of unpasteurized milk or dairy products in or from endemic regions. Although eradicated from li…
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Control of bovine brucellosis from 1998 to 2009 in the State of Mato Grosso do Sul, Brazil Open
This study used disease prevalence as an indicator to assess the effectiveness of the bovine brucellosis vaccination program implemented by the state of Mato Grosso do Sul. The state was divided into three regions: Pantanal, Planalto Sul, …
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Development of new generation of vaccines for Brucella abortus Open
Brucella abortus is a Gram-negative facultative and intracellular bacteria, it causes bovine brucellosis, a zoonotic disease that is responsible for considerable economic loss to owners of domesticated animals and can cause problems in oth…
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A T4SS Effector Targets Host Cell Alpha-Enolase Contributing to Brucella abortus Intracellular Lifestyle Open
Brucella abortus, the causative agent of bovine brucellosis, invades and replicates within cells inside a membrane-bound compartment known as the Brucella containing vacuole (BCV). After trafficking along the endocytic and secretory pathwa…
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<i>Notes from the Field:</i> Human <i>Brucella abortus</i> RB51 Infections Caused by Consumption of Unpasteurized Domestic Dairy Products — United States, 2017–2019 Open
Since August 2017, CDC has confirmed three cases of brucellosis attributed to Brucella abortus cattle vaccine strain RB51. Each case was associated with consumption of domestically acquired unpasteurized (raw) milk products.
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An evaluation of serological tests in the diagnosis of bovine brucellosis in naturally infected cattle in KwaZulu-Natal province in South Africa Open
The diagnostic sensitivity (DSe) of the Rose Bengal test (RBT), the complement fixation test (CFT), the serum agglutination test (SAT), the competitive enzyme-linked immunosorbent assay (cELISA) and the indirect ELISA (iELISA) were determi…
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Bayesian Estimation of Sensitivity and Specificity of Rose Bengal, Complement Fixation, and Indirect ELISA Tests for the Diagnosis of Bovine Brucellosis in Ethiopia Open
Test evaluation in the absence of a gold standard test was conducted for the diagnosis and screening of bovine brucellosis using three commercially available tests including RBPT, CFT, and I-ELISA in National Animal Health Diagnostic and I…
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Glial Cell–Elicited Activation of Brain Microvasculature in Response to <i>Brucella abortus</i> Infection Requires ASC Inflammasome–Dependent IL-1β Production Open
Blood–brain barrier activation and/or dysfunction are a common feature of human neurobrucellosis, but the underlying pathogenic mechanisms are largely unknown. In this article, we describe an immune mechanism for inflammatory activation of…
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Study of serum bactericidal and splenic activity of Total-OMP- CagA combination from Brucella abortus and Helicobacter pylori in BALB/c mouse model Open
Our results showed that the combination vaccine was effective against an oral exposure and the bacterial burden in the spleen, serum and gastric tissues were reduced in mice immunized with the Total- OMP-CagA.
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Isolation & characterization of Brucella melitensis isolated from patients suspected for human brucellosis in India Open
Background & objectives: Brucellosis is endemic in the southern part of India. A combination of biochemical, serological and molecular methods is required for identification and biotyping of Brucella . The present study describes the isola…
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Effect of vaccination in lowering the prevalence of bovine brucellosis in the State of Mato Grosso, Brazil Open
The study was conducted to verify the efficacy of the bovine brucellosis vaccination program implemented by the state of Mato Grosso, using prevalence as an indicator. The state was divided into four regions: Pantanal, Milk, Fattening, and…
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Highly Sensitive Bacteriophage-Based Detection of Brucella abortus in Mixed Culture and Spiked Blood Open
For decades, bacteriophages (phages) have been used for Brucella species identification in the diagnosis and epidemiology of brucellosis. Traditional Brucella phage typing is a multi-day procedure including the isolation of a pure culture,…
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Origins and global context of Brucella abortus in Italy Open
Although related strains exist worldwide, B. abortus isolates from Italy are substantially different than those found in much of the rest of Europe and North America, and are more closely related to strains from the Middle East and Asia. O…
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Prevalence of bovine brucellosis in slaughtered cattle and barriers to better protection of abattoir workers in Ibadan, South-Western Nigeria Open
Our findings indicate moderate prevalence (7.8%) of bovine brucellosis with sex of cattle being a risk factor. A notable barrier to better protection of abattoir workers against brucellosis is perceived inconvenience arising from use of gl…