Spin trapping
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Free Radical Scavenging and Cellular Antioxidant Properties of Astaxanthin Open
Astaxanthin is a coloring agent which is used as a feed additive in aquaculture nutrition. Recently, potential health benefits of astaxanthin have been discussed which may be partly related to its free radical scavenging and antioxidant pr…
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Unveiling singlet oxygen spin trapping in catalytic oxidation processes using in situ kinetic EPR analysis Open
Singlet oxygen ( 1 O 2 ) plays a pivotal role in numerous catalytic oxidation processes utilized in water purification and chemical synthesis. The spin-trapping method based on electron paramagnetic resonance (EPR) analysis is commonly emp…
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Free-Radical Generation from Bulk Nanobubbles in Aqueous Electrolyte Solutions: ESR Spin-Trap Observation of Microbubble-Treated Water Open
Microbubbles are very fine bubbles that shrink and collapse underwater within several minutes, leading to the generation of free radicals. Electron spin resonance spectroscopy (ESR) confirmed the generation of hydroxyl radicals under stron…
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Mechanisms for ·O2- and ·OH Production on Flowerlike BiVO4 Photocatalysis Based on Electron Spin Resonance Open
Many studies have focused on the use of BiVO4 as a photocatalyst, but few have investigated the production of free radicals during the photocatalytic process. Following synthesis of flowerlike BiVO4 and characterization by X-ray diffractio…
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Imaging free radicals in organelles, cells, tissue, and in vivo with immuno-spin trapping Open
The accurate and sensitive detection of biological free radicals in a reliable manner is required to define the mechanistic roles of such species in biochemistry, medicine and toxicology. Most of the techniques currently available are eith…
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Are Radicals Formed During Anion-Exchange Membrane Fuel Cell Operation? Open
In this paper we present a study on stable radicals and short-lived species generated in anion-exchange membrane (AEM) fuel cells (AEMFCs) during operation. The in situ measurements are performed with a micro-AEMFC inserted into a resonato…
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Acid Is Key to the Radical-Trapping Antioxidant Activity of Nitroxides Open
Persistent dialkylnitroxides (e.g., 2,2,6,6-tetramethylpiperidin-1-oxyl, TEMPO) play a central role in the activity of hindered amine light stabilizers (HALS)-additives that inhibit the (photo)oxidative degradation of consumer and industri…
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Reduced Graphene Oxide as a Metal‐Free Catalyst for the Light‐Assisted Fenton‐Like Reaction Open
Natural sunlight irradiation increases the catalytic activity of reduced graphene oxide (rGO) as metal‐free Fenton‐like catalyst for phenol degradation. Generation of hydroxyl radical intermediates was confirmed by EPR spectroscopy using a…
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Free Radical Production and Characterization of Heat-Not-Burn Cigarettes in Comparison to Conventional and Electronic Cigarettes Open
With conventional cigarettes, the burning cone reaches temperatures of >900 °C, resulting in the production of numerous toxicants and significant levels of highly reactive free radicals. In attempts to eliminate combustion while still deli…
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Review—Quantification of Hydrogen Peroxide by Electrochemical Methods and Electron Spin Resonance Spectroscopy Open
Reactive oxygen species (ROS) exhibit different spatial and temporal distributions as well as concentrations in- and outside the cell, therebyfunctioningassignalingorpathogen-destroyingmolecules.EspeciallytheROSH2O2 isimportantforthepatho/…
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Electron Paramagnetic Resonance for the Detection of Electrochemically Generated Hydroxyl Radicals: Issues Associated with Electrochemical Oxidation of the Spin Trap Open
For the detection of electrochemically produced hydroxyl radicals (HO·) from the oxidation of water on a boron-doped diamond (BDD) electrode, electron paramagnetic resonance spectroscopy (EPR) in combination with spin trap labels is a popu…
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Online Quantification of Criegee Intermediates of α-Pinene Ozonolysis by Stabilization with Spin Traps and Proton-Transfer Reaction Mass Spectrometry Detection Open
Biogenic alkenes, which are among the most abundant volatile organic compounds in the atmosphere, are readily oxidized by ozone. Characterizing the reactivity and kinetics of the first-generation products of these reactions, carbonyl oxide…
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Physiological and pathophysiological reactive oxygen species as probed by EPR spectroscopy: the underutilized research window on muscle ageing Open
Reactive oxygen and nitrogen species (ROS and RNS) play crucial roles in triggering, mediating and regulating physiological and pathophysiological signal transduction pathways within the cell. Within the cell, ROS efflux is firmly controll…
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Comparison of different methods for measuring the superoxide radical by EPR spectroscopy in buffer, cell lysates and cells Open
As superoxide anion is of keen interest in biomedical research, it is highly desirable to have a technique allowing its detection sensitively and specifically in biological media. If electron paramagnetic resonance (EPR) techniques and pro…
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Role of the Hydroxyl Radical-Generating System in the Estimation of the Antioxidant Activity of Plant Extracts by Electron Paramagnetic Resonance (EPR) Open
The scavenging activity of hydroxyl radicals, produced by the Fenton reaction, is commonly used to quantify the antioxidant capacity of plant extracts. In this study, three Fenton systems (Fe/phosphate buffer, Fe/quinolinic acid and Fe/pho…
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Photoactivity properties of ZnO doped with cerium ions: an EPR study Open
In the present study, we investigated the effect of cerium doping in zinc oxide matrix, used as photoactive material. Cerium ions into the matrix of ZnO can act like a 'trap' for the electrons, lowering the e -/h + recombination rate and s…
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Electron Paramagnetic Resonance Spin Trapping (EPR–ST) Technique in Photopolymerization Processes Open
To face economic issues of the last ten years, free-radical photopolymerization (FRP) has known an impressive enlightenment. Multiple performing photoinitiating systems have been designed to perform photopolymerizations in the visible or n…
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Operando EPR Study of Radical Formation in Anion-Exchange Membrane Fuel Cells Open
The alkaline operating environment of anion-exchange membrane (AEM) fuel cells (AEMFCs) makes it possible to employ a wide variety of catalysts, including platinum group metals (PGMs) as well as PGM-free materials. However, little is under…
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Hydroxyl Radical Generation by the H2O2/CuII/Phenanthroline System under Both Neutral and Alkaline Conditions: An EPR/Spin-Trapping Investigation Open
The copper–phenanthroline complex CuI(Phen)2 was the first artificial nuclease studied in biology. The mechanism responsible for this activity involves CuII(Phen)2 and H2O2. Even if H2O2/Cu systems have been extensively studied in biology …
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N-tert-butylmethanimine N-oxide is an efficient spin-trapping probe for EPR analysis of glutathione thiyl radical Open
The electron spin resonance (EPR) spin-trapping technique allows detection of radical species with nanosecond half-lives. This technique is based on the high rates of addition of radicals to nitrones or nitroso compounds (spin traps; STs).…
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Electron paramagnetic resonance (EPR) for investigating relevant players of redox reactions: Radicals, metalloproteins and transition metal ions Open
Electron paramagnetic resonance (EPR) spectroscopy is unique in providing robust information about free radicals, transition metal ions and metalloenzymes, which are crucial players in redox processes. EPR had a major role in advancing the…
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Copper(II) Thiosemicarbazone Complexes and Their Proligands upon UVA Irradiation: An EPR and Spectrophotometric Steady-State Study Open
X- and Q-band electron paramagnetic resonance (EPR) spectroscopy was used to characterize polycrystalline Cu(II) complexes that contained sodium 5-sulfonate salicylaldehyde thiosemicarbazones possessing a hydrogen, methyl, ethyl, or phenyl…
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Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K Open
The accurate and specific detection of reactive oxygen species (ROS) in different cellular and tissue compartments is essential to the study of redox-regulated signaling in biological settings. Electron paramagnetic resonance spectroscopy …
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Inhibition of phagocytic activity of ARPE-19 cells by free radical mediated oxidative stress Open
Oxidative stress is a main factor responsible for key changes leading to the onset of age-related macular degeneration (ARMD) that occur in the retinal pigment epithelium (RPE), which is involved in phagocytosis of photoreceptor outer segm…
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Green and Effective Removal of Aqueous Graphene Oxide under UV-Light Irradiation Open
The potential extensive application of graphene oxide (GO) in various fields results in the possibility of its release into the natural environment with negative impacts on humans and the ecosystem. The UV-induced removal behavior of aqueo…
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Antiproliferative activity of novel isatinyl/indanyl nitrones (INs) as potential spin trapping agents of free radical intermediates Open
Isatinyl and indanyl nitrones (INs) were synthesized and evaluated for their antiproliferative activity and antioxidant properties.
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Use of a cocktail of spin traps for fingerprinting large range of free radicals in biological systems Open
It is well established that the formation of radical species centered on various atoms is involved in the mechanism leading to the development of several diseases or to the appearance of deleterious effects of toxic molecules. The detectio…
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On the Effect of the Internal Pressure on the Photoinduced Spin‐Crossover Behavior of [Fe<i><sub>x</sub></i>M<sub>1–<i>x</i></sub>(1,10‐phenanthroline)<sub>2</sub>(NCS)<sub>2</sub>] Solid Solutions (M = Ni<sup>II</sup>, Zn<sup>II</sup>, and Cd<sup>II</sup>) Open
The thermal and light‐induced magnetic properties of a family of metal‐diluted spin‐crossover complexes, [Fe x M 1– x (phen) 2 (NCS) 2 ] (phen = 1,10‐phenantholine and M = Ni II , Cd II ), are reported and compared to previous results obta…
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Synthesis, characterization, and application of 2D/2D TiO2-GO-ZnFe2O4 obtained by the fluorine-free lyophilization method for solar light-driven photocatalytic degradation of ibuprofen Open
In this study, we report the potential of 2D/2D TiO 2 -GO-ZnFe 2 O 4 photocatalyst obtained using the fluorine-free lyophilization technique for the degradation of ibuprofen belonging to the group of active pharmaceutical ingredients (API)…
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<i>In Vivo</i> and <i>In Situ</i> Detection of Macromolecular Free Radicals Using Immuno-Spin Trapping and Molecular Magnetic Resonance Imaging Open
The approach can also be used to assess therapeutic agents that are either free radical scavengers or generate free radicals. Smaller probe constructs and radical identification approaches are being considered. The focus of this review is …