Nicotiana benthamiana
View article: A Multidrug and Toxic Compound Extrusion Transporter, RgMATE6, Facilitates Vacuolar Transport of Acteoside in Rehmannia glutinosa
A Multidrug and Toxic Compound Extrusion Transporter, RgMATE6, Facilitates Vacuolar Transport of Acteoside in Rehmannia glutinosa Open
Acteoside (ACT), a prominent compound of the hydroxytyrosol-type phenylethanol glycoside (HPG) class, is present in plants and holds significant potential for food and pharmaceutical applications. However, the limited production of ACT in …
View article: An innovative infection method for the accumulation of viral nanoparticles in Nicotiana benthamiana
An innovative infection method for the accumulation of viral nanoparticles in Nicotiana benthamiana Open
Tomato Bushy Stunt Virus (TBSV) naturally infects tomato plants, although it can also infect other plant species, such as Nicotiana benthamiana , a known model system in plant molecular farming. In the presented work, a novel system for TB…
View article: A Critical Commentary on "Two residues reprogram immunity receptor kinases to signal in nitrogen-fixing symbiosis" by Tsitsikli et al., Nature 2025; doi: 10.1038/s41586-025-09696-3
A Critical Commentary on "Two residues reprogram immunity receptor kinases to signal in nitrogen-fixing symbiosis" by Tsitsikli et al., Nature 2025; doi: 10.1038/s41586-025-09696-3 Open
This repository contains a comprehensive, critical re-evaluation of the research article “Two residues reprogram immunity receptors for nitrogen-fixing symbiosis” by Tsitsikli et al., Nature 2025. Our commentary—authored by Mengxi Zhu and …
View article: Conserved early steps of stemmadenine biosynthesis
Conserved early steps of stemmadenine biosynthesis Open
Stemmadenine acetate is a pivotal intermediate in the production of pharmacologically active monoterpene indole alkaloids. Here, we identify orthologs of stemmadenine acetate pathway genes (SGD, GS, GO, Redox1, Redox2, SAT) from Tabernanth…
View article: Cytokinin-mediated trichome initiation in <i>Nicotiana benthamiana</i> upon <i>Agrobacterium tumefaciens</i> infiltration
Cytokinin-mediated trichome initiation in <i>Nicotiana benthamiana</i> upon <i>Agrobacterium tumefaciens</i> infiltration Open
Infiltration of Agrobacterium tumefaciens in leaves of Nicotiana benthamiana has become a standard method for transient expression in plants. Like many Solanaceae species, N. benthamiana develops on its leaf surface capitate glandular tric…
View article: Research on the Pathogenic Mechanism of Effector FvCfem7 in Fusarium verticillioides
Research on the Pathogenic Mechanism of Effector FvCfem7 in Fusarium verticillioides Open
Fusarium verticillioides, a hemibiotrophic pathogen, infects a range of important crops and contaminates grains with fumonisin B1 (FB1) toxins, posing serious threats to yield, quality, and food safety. Secreted proteins containing Common …
View article: Four Petal-Specific TPS Drive Nocturnal Terpene Scent in <em>Jasminum sambac</em>
Four Petal-Specific TPS Drive Nocturnal Terpene Scent in <em>Jasminum sambac</em> Open
Floral volatile terpenoids are known to play important roles in plant pollination biology by attracting animal pollinators and repelling antagonists, as well as enhancing resistance to potential microbial pathogens. Terpenoid blend emitted…
View article: Identification, Subcellular Localization, and Infection-Related Expression of a Novel Haloacid Dehalogenase Gene (VmHAD) from Valsa mali Vm1
Identification, Subcellular Localization, and Infection-Related Expression of a Novel Haloacid Dehalogenase Gene (VmHAD) from Valsa mali Vm1 Open
The haloacid dehalogenase (HAD) superfamily represents a large group of enzymes across diverse taxa. However, the characteristics and functional roles of HAD members in the destructive apple canker pathogen, Valsa mali strain Vm1 (Vm1), re…
View article: Grapevine protein Src2 mediates plant disease resistance during <i>Lasiodiplodia theobromae</i> infection
Grapevine protein Src2 mediates plant disease resistance during <i>Lasiodiplodia theobromae</i> infection Open
Lysin motif (LysM) effectors contribute to the virulence of many pathogens, but the underlying mechanism of this type of effector remains poorly understood. Here, we identified a LysM-containing protein, named LtLysM2, in the plant opportu…
View article: Xyloglucan Endotransglycosylase/Hydrolase Downregulation Increases Nicotiana benthamiana Tolerance to Tobacco Mosaic Virus Infection
Xyloglucan Endotransglycosylase/Hydrolase Downregulation Increases Nicotiana benthamiana Tolerance to Tobacco Mosaic Virus Infection Open
The biological functions of the multiple members of the xyloglucan endotransglycosylase/hydrolase (XTH) protein family are rather diverse: XTHs are cell wall remodeling enzymes that participate in plant growth and development, are involved…
View article: Peptide AEDL Activates Metabolism and Autophagy in Root Cells of Nicotiana tabacum
Peptide AEDL Activates Metabolism and Autophagy in Root Cells of Nicotiana tabacum Open
The short peptide AlaGluAspLeu (AEDL) stimulates shoot and root development in Nicotiana tabacum. Growing tobacco in the presence of AEDL was found to induce autophagy and programmed cell death, as demonstrated using immunodetection of the…
View article: Development of convenient potyvirus-based vectors for simultaneous expression of two heterologous proteins in solanaceous plants
Development of convenient potyvirus-based vectors for simultaneous expression of two heterologous proteins in solanaceous plants Open
Plant virus-based vectors can deliver foreign nucleic acid sequences into host plants for expression, serving as powerful biotechnological tools in agricultural applications, such as plant functional genomic study, agronomic trait improvem…
View article: Efficient production of functional cholera toxin B subunit using geminiviral replicons in Nicotiana benthamiana
Efficient production of functional cholera toxin B subunit using geminiviral replicons in Nicotiana benthamiana Open
The cholera toxin B subunit (CTB) has the potential to be a carrier molecule and an effective adjuvant for mucosal vaccines because of its ability to enhance immune responses to antigens. CTB proteins have been expressed in plant-based exp…
View article: Replication and Movement of In Vitro-Generated Infectious RNAs of Potato Mop-Top Virus RNAs in Mechanically Inoculated Two Potato Cultivars
Replication and Movement of In Vitro-Generated Infectious RNAs of Potato Mop-Top Virus RNAs in Mechanically Inoculated Two Potato Cultivars Open
Potato mop-top virus (PMTV), vectored by soil-borne protist Spongospora subterranea f. sp. subterranea (Sss) is increasingly becoming a threat to potato production in the USA. Major means of spread is through infected tubers, vector, and c…
View article: Functional analysis of NtMYB15 from Narcissus tazetta var. chinensis in transgenic aromatic plants (Petunia hybrida and Nicotiana tabacum) regulating the colour and aroma of the flowers
Functional analysis of NtMYB15 from Narcissus tazetta var. chinensis in transgenic aromatic plants (Petunia hybrida and Nicotiana tabacum) regulating the colour and aroma of the flowers Open
View article: Identification and Characterization of the Delta-12 Fatty Acid Desaturase from Euglena gracilis
Identification and Characterization of the Delta-12 Fatty Acid Desaturase from Euglena gracilis Open
Fatty acid desaturase 12 (FAD12) is a key enzyme in fatty acid biosynthesis, responsible for converting oleic acid to linoleic acid through desaturase activity. Euglena gracilis (Euglena) is an emerging platform for the industrial producti…
View article: Mutualist-pathogen co-colonisation modulates phosphoinositide signatures at host intracellular interfaces
Mutualist-pathogen co-colonisation modulates phosphoinositide signatures at host intracellular interfaces Open
View article: Integrated Omics Dataset of Bamboo mosaic virus (BaMV)–Infected Nicotiana benthamiana Leaves
Integrated Omics Dataset of Bamboo mosaic virus (BaMV)–Infected Nicotiana benthamiana Leaves Open
This dataset supports the study investigating virus-induced metabolic reprogramming and redox regulation during Bamboo mosaic virus (BaMV) infection in Nicotiana benthamiana. It includes raw and processed data from metabolomic, metabolic f…
View article: Integrated Omics Dataset of Bamboo mosaic virus (BaMV)–Infected Nicotiana benthamiana Leaves
Integrated Omics Dataset of Bamboo mosaic virus (BaMV)–Infected Nicotiana benthamiana Leaves Open
This dataset supports the study investigating virus-induced metabolic reprogramming and redox regulation during Bamboo mosaic virus (BaMV) infection in Nicotiana benthamiana. It includes raw and processed data from metabolomic, me…
View article: CRISPR/Cas9‐Mediated Knockouts of the <i>ALG3</i> and <i>GNTI</i> in <i>N. benthamiana</i> and Their Application to Pharmaceutical Production
CRISPR/Cas9‐Mediated Knockouts of the <i>ALG3</i> and <i>GNTI</i> in <i>N. benthamiana</i> and Their Application to Pharmaceutical Production Open
N ‐Glycosylation critically influences the efficacy, safety and pharmacokinetic properties of biopharmaceuticals. Plant expression platforms offer multiple advantages for the production of N ‐glycosylated proteins, but their use is impeded…
View article: A CNL protein forms an NLR pair with NRCX to modulate plant immunity
A CNL protein forms an NLR pair with NRCX to modulate plant immunity Open
Nucleotide-binding leucine-rich repeat (NLR) proteins assemble into genetically linked pairs to mediate effector-triggered immunity (ETI) in plants. Here, we characterize the paired NLRs NRCX and NARY (NRCX adjacent resistance gene Y) in N…
View article: Heterologous Production of Cyprosin B in <i>Nicotiana benthamiana</i>: Unveiling the Role of the Plant‐Specific Insert Domain in Protein Function and Subcellular Localisation
Heterologous Production of Cyprosin B in <i>Nicotiana benthamiana</i>: Unveiling the Role of the Plant‐Specific Insert Domain in Protein Function and Subcellular Localisation Open
Plant systems have gained increased attention as an alternative platform for producing heterologous proteins, particularly for industrially relevant proteins. The Cynara cardunculus L. flower extract is traditionally used in cheese product…
View article: Transcriptomic changes associated with infection of Nicotiana benthamiana plants with tomato ringspot virus (genus Nepovirus) during the acute symptomatic stage and after symptom recovery
Transcriptomic changes associated with infection of Nicotiana benthamiana plants with tomato ringspot virus (genus Nepovirus) during the acute symptomatic stage and after symptom recovery Open
We have characterized the transcriptome of Nicotiana benthamiana plants infected with tomato ringspot virus (ToRSV), a nepovirus. We analyzed two different stages of infection: the acute systemic symptomatic stage and the symptom recovery …
View article: Intron hairpin RNA derived from the HC-Pro gene confers resistance to papaya ringspot virus in transgenic Nicotiana benthamiana
Intron hairpin RNA derived from the HC-Pro gene confers resistance to papaya ringspot virus in transgenic Nicotiana benthamiana Open
In the present study, cDNA from a papaya leaf sample infected with Papaya Ringspot Virus (PRSV) was used for the amplification of the HC-Pro gene, which yielded an expected amplicon size of ~1350 bp. The partial coding sequence of HC-Pro w…
View article: Analysis of salivary proteins in gall-inducing psylla and their potential influence on host plants
Analysis of salivary proteins in gall-inducing psylla and their potential influence on host plants Open
View article: Genome-Wide Identification of the Glycosyl Hydrolase Family 1 Genes in Brassica napus L. and Functional Characterization of BnBGLU77
Genome-Wide Identification of the Glycosyl Hydrolase Family 1 Genes in Brassica napus L. and Functional Characterization of BnBGLU77 Open
The β-glucosidases (BGLUs) of Glycoside Hydrolase Family 1 (GH1) exhibit essential functions in plant secondary metabolism and stress responses, mediated by their dual catalytic capabilities in hydrolysis and transglycosylation. This study…
View article: Coordinated actions of NLR-assembled and glutamate receptor–like calcium channels in plant effector-triggered immunity
Coordinated actions of NLR-assembled and glutamate receptor–like calcium channels in plant effector-triggered immunity Open
The plant immune system utilizes nucleotide-binding/leucine-rich repeat (NLR) proteins to detect pathogen virulence factors (effectors) inside host cells and transduce recognition to rapid defense. In dicotyledenous plants, pathogen activa…
View article: Receptor-like kinase SlRLK-like positively regulates sugar accumulation and fruit ripening in tomato
Receptor-like kinase SlRLK-like positively regulates sugar accumulation and fruit ripening in tomato Open
Introduction The ripening process of tomato fruits involves many complex changes. The elucidation of the ripening pathways contributes to the reduction of post-harvest losses and improvement of fruit quality. However, much is unknown about…
View article: Transient expression of human interleukin-15 in <i>Nicotiana benthamiana</i>
Transient expression of human interleukin-15 in <i>Nicotiana benthamiana</i> Open
View article: MdCDPK24 Encoding Calcium-Dependent Protein Kinase Enhances Apple Resistance to Colletotrichum gloeosporioides
MdCDPK24 Encoding Calcium-Dependent Protein Kinase Enhances Apple Resistance to Colletotrichum gloeosporioides Open
Calcium-dependent protein kinases (CDPKs) are unique serine/threonine kinases that play significant roles in response to environmental stresses in plants. In this study, we comprehensively characterized the CDPK gene family in the apple cu…