Aldolase A
View article: Multigene manipulation of photosynthetic carbon assimilation increases CO2 fixation and biomass yield in tobacco
Multigene manipulation of photosynthetic carbon assimilation increases CO2 fixation and biomass yield in tobacco Open
Over the next 40 years it has been estimated that a 50% increase in the yield of grain crops such as wheat and rice will be required to meet the food and fuel demands of the increasing world population. Transgenic tobacco plants have been …
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Simultaneous stimulation of sedoheptulose 1,7‐bisphosphatase, fructose 1,6‐bisphophate aldolase and the photorespiratory glycine decarboxylase‐H protein increases <span>CO</span><sub>2</sub> assimilation, vegetative biomass and seed yield in Arabidopsis Open
Summary In this article, we have altered the levels of three different enzymes involved in the Calvin–Benson cycle and photorespiratory pathway. We have generated transgenic Arabidopsis plants with altered combinations of sedoheptulose 1,7…
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Role of Conformational Dynamics in the Evolution of Retro-Aldolase Activity Open
Enzymes exist as ensembles of conformations that are important for function. Tuning these populations of conformational states through mutation enables evolution toward additional activities. Here we computationally evaluate the population…
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Revisited Metabolic Control and Reprogramming Cancers by Means of the Warburg Effect in Tumor Cells Open
Aerobic glycolysis is an emerging hallmark of many human cancers, as cancer cells are defined as a “metabolically abnormal system”. Carbohydrates are metabolically reprogrammed by its metabolizing and catabolizing enzymes in such abnormal …
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Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice Open
Increasing evidence suggests a role for excessive intake of fructose in the Western diet as a contributor to the current epidemics of metabolic syndrome and obesity. Hereditary fructose intolerance (HFI) is a difficult and potentially leth…
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Loss‐of‐Function Genetic Screening Identifies Aldolase A as an Essential Driver for Liver Cancer Cell Growth Under Hypoxia Open
Background and Aims Hypoxia is a common feature of the tumor microenvironment (TME), which promotes tumor progression, metastasis, and therapeutic drug resistance through a myriad of cell activities in tumor and stroma cells. While targeti…
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Zinc disrupts central carbon metabolism and capsule biosynthesis in Streptococcus pyogenes Open
Neutrophils release free zinc to eliminate the phagocytosed bacterial pathogen Streptococcus pyogenes (Group A Streptococcus ; GAS). In this study, we investigated the mechanisms underpinning zinc toxicity towards this human pathogen, resp…
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Molecular Characterization, Gene Evolution, and Expression Analysis of the Fructose-1, 6-bisphosphate Aldolase (FBA) Gene Family in Wheat (Triticum aestivum L.) Open
Fructose-1, 6-bisphosphate aldolase (FBA) is a key plant enzyme that is involved in glycolysis, gluconeogenesis, and the Calvin cycle. It plays significant roles in biotic and abiotic stress responses, as well as in regulating growth and d…
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Proteomic analysis reveals dynamic regulation of fruit development and sugar and acid accumulation in apple Open
Understanding the fruit developmental process is critical for fruit quality improvement. Here, we report a comprehensive proteomic analysis of apple fruit development over five growth stages, from young fruit to maturity, coupled with meta…
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Aldolase B suppresses hepatocellular carcinogenesis by inhibiting G6PD and pentose phosphate pathways Open
Metabolic reprogramming is a core hallmark of cancer but it remains poorly defined in hepatocellular carcinogenesis (HCC). Here we show that hepatic aldolase B (Aldob) suppresses HCC by directly binding and inhibiting the rate-limiting enz…
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Multifunctional Fructose 1,6-Bisphosphate Aldolase as a Therapeutic Target Open
Fructose 1,6-bisphosphate aldolase is a ubiquitous cytosolic enzyme that catalyzes the fourth step of glycolysis. Aldolases are classified into three groups: Class-I, Class-IA, and Class-II; all classes share similar structural features bu…
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Lactylome Analysis Unveils Lactylation‐Dependent Mechanisms of Stemness Remodeling in the Liver Cancer Stem Cells Open
Lactate plays a critical role as an energy substrate, metabolite, and signaling molecule in hepatocellular carcinoma (HCC). Intracellular lactate‐derived protein lysine lactylation (Kla) is identified as a contributor to the progression of…
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Therapeutic Targeting of Aldolase A Interactions Inhibits Lung Cancer Metastasis and Prolongs Survival Open
Cancer metabolic reprogramming promotes tumorigenesis and metastasis; however, the underlying molecular mechanisms are still being uncovered. In this study, we show that the glycolytic enzyme aldolase A (ALDOA) is a key enzyme involved in …
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The aldolase inhibitor aldometanib mimics glucose starvation to activate lysosomal AMPK Open
The activity of 5′-adenosine monophosphate-activated protein kinase (AMPK) is inversely correlated with the cellular availability of glucose. When glucose levels are low, the glycolytic enzyme aldolase is not bound to fructose-1,6-bisphosp…
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Structure–Function Relationships between Aldolase C/Zebrin II Expression and Complex Spike Synchrony in the Cerebellum Open
Simple and regular anatomical structure is a hallmark of the cerebellar cortex. Parasagittally arrayed alternate expression of aldolase C/zebrin II in Purkinje cells (PCs) has been extensively studied, but surprisingly little is known abou…
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Entner–Doudoroff pathway for sulfoquinovose degradation in <i>Pseudomonas putida</i> SQ1 Open
Significance Phototrophic organisms worldwide produce estimated 10 gigatons of sulfoquinovose (SQ) per year; hence, complete degradation of SQ by bacteria is an important part of the biogeochemical sulfur cycle. Here, we show that Pseudomo…
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Fructose-bisphosphate aldolase A is a key regulator of hypoxic adaptation in colorectal cancer cells and involved in treatment resistance and poor prognosis Open
Hypoxia is an essential feature of cancer malignancy, but there are no methods for the routine detection of hypoxia-inducible prognostic factors and potential therapeutic targets. We reported previously that the hypoxic tumor cells of meta…
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Combining Aldolases and Transaminases for the Synthesis of 2-Amino-4-hydroxybutanoic Acid Open
Amino acids are of paramount importance as chiral building blocks of life, for drug development in modern medicinal chemistry, and for the manufacture of industrial products. In this work, the stereoselective synthesis of (S)- and (R)-2-am…
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Rewiring of embryonic glucose metabolism via suppression of PFK-1 and aldolase during mouse chorioallantoic branching Open
Adapting the energy metabolism state to changing bioenergetic demands is essential for mammalian development accompanying massive cell proliferation and cell differentiation. However, it remains unclear how developing embryos meet the chan…
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Aldolase A promotes proliferation and G<sub>1</sub>/S transition via the EGFR/MAPK pathway in non‐small cell lung cancer Open
Background Our previous study demonstrated that aldolase A (ALDOA) is overexpressed in clinical human lung squamous cell carcinoma and that ALDOA promotes epithelial–mesenchymal transition and tumorigenesis. The present study aimed to expl…
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Accelerating Enzymatic Catalysis Using Vortex Fluidics Open
Enzymes catalyze chemical transformations with outstanding stereo‐ and regio‐specificities, but many enzymes are limited by their long reaction times. A general method to accelerate enzymes using pressure waves contained within thin films …
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Efficient laboratory evolution of computationally designed enzymes with low starting activities using fluorescence-activated droplet sorting Open
De novo biocatalysts with non-natural functionality are accessible by computational enzyme design. The catalytic activities obtained for the initial designs are usually low, but can be optimized significantly by directed evolution. Neverth…
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Enzymatic One‐Step Reduction of Carboxylates to Aldehydes with Cell‐Free Regeneration of ATP and NADPH Open
The direct generation of aldehydes from carboxylic acids is often a challenging synthetic task but undoubtedly attractive in view of abundant supply of such feedstocks from nature. Though long known, biocatalytic carboxylate reductions are…
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Mechanism-based inhibition of an aldolase at high concentrations of its natural substrate acetaldehyde: structural insights and protective strategies Open
Understanding the deactivation mechanism of 2-deoxy- d -ribose-5-phosphate aldolase by its natural substrate leads to a single mutant showing complete acetaldehyde resistance.
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Regulation of Polyhydroxybutyrate Synthesis in the Soil Bacterium Bradyrhizobium diazoefficiens Open
Polyhydroxybutyrate (PHB) is a carbon and energy reserve polymer in various prokaryotic species. We determined that, when grown with mannitol as the sole carbon source, Bradyrhizobium diazoefficiens produces a homopolymer composed only of …
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Flavonoids as a Natural Treatment Against Entamoeba histolytica Open
Over the past 20 years, gastrointestinal infections in developing countries have been a serious health problem and are the second leading cause of morbidity among all age groups. Among pathogenic protozoans that cause diarrheal disease, th…
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Aldolase A promotes epithelial‐mesenchymal transition to increase malignant potentials of cervical adenocarcinoma Open
Recent studies have revealed that metabolic reprogramming is closely associated with epithelial‐mesenchymal transition (EMT) during cancer progression. Aldolase A (ALDOA) is a key glycolytic enzyme that is highly expressed in several types…
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Beef tenderness and intramuscular fat proteomic biomarkers: muscle type effect Open
Tenderness and intramuscular fat content are key attributes for beef sensory qualities. Recently some proteomic analysis revealed several proteins which are considered as good biomarkers of these quality traits. This study focuses on the a…
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Excretion of cytoplasmic proteins (<span>ECP</span>) in <span><i>S</i></span><i>taphylococcus aureus</i> Open
Summary E xcretion of c ytoplasmic p roteins ( ECP ) is a common physiological feature in bacteria and eukaryotes. However, how these proteins without a typical signal peptide are excreted in bacteria is poorly understood. We studied the e…
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Minimalist Protein Engineering of an Aldolase Provokes Unprecedented Substrate Promiscuity Open
Application of aldolases for the asymmetric synthesis of multifunctional chiral products is hampered by their reputed strict nucleophile (=aldol donor) specificity owing to a mechanistic requirement for creating a carbanion nucleophile in …