Intravital microscopy
View article: In vivo volumetric visualization and quantification of cumulus expansion in mice with intravital optical coherence tomography
In vivo volumetric visualization and quantification of cumulus expansion in mice with intravital optical coherence tomography Open
View article: μLectures: Intravital microscopy
μLectures: Intravital microscopy Open
This presentation is part of the μLecture series about useful microscopy-related topics, organized by the Microscopy Service Centre, IEM CAS. It explores the use of live animals in microscopy and introduces intravital imaging as a powerful…
View article: μLectures: Intravital microscopy
μLectures: Intravital microscopy Open
This presentation is part of the μLecture series about useful microscopy-related topics, organized by the Microscopy Service Centre, IEM CAS. It explores the use of live animals in microscopy and introduces intravital imaging as a powerful…
View article: Stable intracranial imaging of dura mater-engrafted pancreatic islet cells in awake mice
Stable intracranial imaging of dura mater-engrafted pancreatic islet cells in awake mice Open
By transplanting pancreatic islets onto the dura mater of the mouse brain, we establish a microscopy platform that enables longitudinal intravital imaging of otherwise optically inaccessible tissue. The system combines a cranial window wit…
View article: Influence of biophysical and biochemical cues on Plasmodium sporozoite dynamics in skin tissue
Influence of biophysical and biochemical cues on Plasmodium sporozoite dynamics in skin tissue Open
View article: Bacterial Induced Calcium Dynamics in Dendritic Cells In Vivo 2225
Bacterial Induced Calcium Dynamics in Dendritic Cells In Vivo 2225 Open
Description Dendritic cells (DCs) are sentinels that orchestrate immune responses to infections, cancer, and tissue damage. DCs play a crucial role bridging the innate and adaptive immune systems, eliciting tailored adaptive immune respons…
View article: Real-time visualization of neutrophil distribution in the thymus following CXCR4 antagonist (plerixafor) treatment using intravital microscopy 3656
Real-time visualization of neutrophil distribution in the thymus following CXCR4 antagonist (plerixafor) treatment using intravital microscopy 3656 Open
Description Plerixafor, a CXCR4 antagonist, is commonly used in clinical treatments and promotes neutrophil production by acting on hematopoietic stem cells. However, current studies on its pharmacokinetics (PK) and mechanisms often rely o…
View article: CNSC-47. REAL-TIME CORRELATIVE MRI AND INTRAVITAL MICROSCOPY REVEAL MICROVASCULAR SIGNATURES IN BRAIN METASTASES
CNSC-47. REAL-TIME CORRELATIVE MRI AND INTRAVITAL MICROSCOPY REVEAL MICROVASCULAR SIGNATURES IN BRAIN METASTASES Open
Understanding brain vasculature in metastases requires correlating MRI resolution limitations with microscopic ground truth. Using the BRIDGE framework, we correlated two-photon (2P) microscopy with MRI (T2, T2*, TOF) to analyze vessel vis…
View article: Highly dynamic dural sinus endothelial cells support meningeal immunity 4052
Highly dynamic dural sinus endothelial cells support meningeal immunity 4052 Open
Description The CNS is covered by three interconnected membranes referred to as the meninges that host a diverse immune network. Situated within the skull-interfacing dura mater are venous sinuses, which are large veins thought to serve pr…
View article: Characterization of age-dependent changes to hemodynamics and thymocyte motility in the native thymus after SL-TBI using two-photon intravital microendoscopy 4457
Characterization of age-dependent changes to hemodynamics and thymocyte motility in the native thymus after SL-TBI using two-photon intravital microendoscopy 4457 Open
Description The thymus, as the primary site of T cell maturation, plays a critical role in the functionality of the adaptive immune system, and as such, the recovery of the thymus after injury and the role that the thymus vasculature syste…
View article: The dual non-competitive CXCR1/2 inhibitor ladarixin impairs neutrophil extravasation without altering intravascular adhesion
The dual non-competitive CXCR1/2 inhibitor ladarixin impairs neutrophil extravasation without altering intravascular adhesion Open
In many acute and chronic inflammatory disorders, recruitment of neutrophils plays a critical role in preventing disease severity and ensuring survival. On the other hand, neutrophil accumulation during inflammation can also favor disease …
View article: Spleen Tyrosine Kinase Inhibition Mitigates Hemin-Induced Thromboinflammation in an Organ-Specific Manner in Sickle Cell Mice
Spleen Tyrosine Kinase Inhibition Mitigates Hemin-Induced Thromboinflammation in an Organ-Specific Manner in Sickle Cell Mice Open
BACKGROUND: Sickle cell disease (SCD) is a challenging genetic disorder characterized by hemolytic anemia, vaso-occlusive crises (VOC), and progressive organ damage. Despite its severity, effective treatments are limited. The recent withdr…
View article: Prospect for studying the vital anatomy and topography of the spleen in humans depending on gender based on computer tomography data
Prospect for studying the vital anatomy and topography of the spleen in humans depending on gender based on computer tomography data Open
The aim of the work is to determine new data on the intravital anatomy of the spleen using computed tomography in patients depending on gender. The computed tomographic anatomy and topography of the spleen were studied based on the analysi…
View article: Real-time Imaging of Vascular Changes and Extravasation in the Chicken Chorioallantoic Membrane (CAM) Model Exposed to Ultrasound and Microbubbles
Real-time Imaging of Vascular Changes and Extravasation in the Chicken Chorioallantoic Membrane (CAM) Model Exposed to Ultrasound and Microbubbles Open
Slow and fast extravasation kinetics could be due to diffusion or ultrasound-induced convection. This study demonstrates that the CAM model is highly useful for studying bioeffects induced by sonopermeation at a vascular level.
View article: A multiscale vascular atlas of blood vessels supplying the urinary bladder of male and female mice
A multiscale vascular atlas of blood vessels supplying the urinary bladder of male and female mice Open
The vasculature of the urinary bladder is embedded within a highly dynamic environment impacted by cycles of voiding and continence, placing unique demands on tissue perfusion and immune surveillance. This study has applied multiscale micr…
View article: Microglial cyclooxygenase-1 modulates cerebral capillary basal tone in vivo in mice
Microglial cyclooxygenase-1 modulates cerebral capillary basal tone in vivo in mice Open
View article: Brain precapillary sphincters modulate myogenic tone in adult and aged mice
Brain precapillary sphincters modulate myogenic tone in adult and aged mice Open
Brain precapillary sphincters, which are surrounded by contractile pericytes and are located at the junction of penetrating arterioles and first-order capillaries, can increase their diameter by ~ 30% in a few seconds during sensory stimul…
View article: Visualizing Vascular Bone Marrow Niche Alterations in Diabetes
Visualizing Vascular Bone Marrow Niche Alterations in Diabetes Open
BACKGROUND: Diabetes is characterized by chronic hyperglycemia that leads to systemic vascular complications. Hyperglycemia impairs endothelial function and promotes vascular inflammation, resulting in leukocytosis, altered hematopoiesis, …
View article: Microendoscopy for periodic intravital end-to-end tumor imaging of cancer cells
Microendoscopy for periodic intravital end-to-end tumor imaging of cancer cells Open
The spatiotemporal heterogeneity in intratumor proliferative behavior of cancer cells deeply affects tumor environment characteristics and the efficacy of anticancer treatments. Thus, intravital imaging with unlimited imaging depth and cel…
View article: Intravital microscopy for assessment of anti-tumor nanotherapeutic delivery
Intravital microscopy for assessment of anti-tumor nanotherapeutic delivery Open
The development of effective relief for cancer is one of the most urgent tasks of biomedicine. Despite the success of anti-tumor nanotherapeutics, low targeted delivery effectiveness remains a major limiting factor for widespread introduct…
View article: PMN recruitment in inflammatory lung injury models follows classical transendothelial migration paradigms requiring PECAM-1 and CD99
PMN recruitment in inflammatory lung injury models follows classical transendothelial migration paradigms requiring PECAM-1 and CD99 Open
Previous studies have shown that neutrophil extravasation in the lung was selectin-independent and the requirement for leukocyte integrins was stimulus-dependent. This study demonstrates that PECAM-1 and CD99 are required for PMN transmigr…
View article: A multi-scale vascular atlas of blood vessels within the urinary bladder of male and female mice
A multi-scale vascular atlas of blood vessels within the urinary bladder of male and female mice Open
The vascular supply of the urinary bladder is embedded within a highly dynamic environment that includes alternating cycles of regional compression or stretching during bladder filling, sustained continence and voiding. These place unique …
View article: Editorial: Community series in intravital microscopy imaging of leukocytes, volume II
Editorial: Community series in intravital microscopy imaging of leukocytes, volume II Open
View article: Protocol for detecting 3D dermal fibroblast cellular dynamics in mice using an intravital imaging technique
Protocol for detecting 3D dermal fibroblast cellular dynamics in mice using an intravital imaging technique Open
View article: Topographic anatomy of the liver of children and adolescents according to intravital imaging
Topographic anatomy of the liver of children and adolescents according to intravital imaging Open
The aim of the study was to establish age- and gender-related patterns of liver topography in children and adolescents based on intravital visualization data for use in determining the skeletotopy of abdominal organs. Material and methods.…
View article: Imaging technology in tracking the intravital fate of transplanted stem cells
Imaging technology in tracking the intravital fate of transplanted stem cells Open
Stem cell therapy emerges as a promising alternative strategy for diseases that currently lack effective treatment options. Investigating the pharmacokinetic properties of stem cells, such as their survival, migration, differentiation, and…
View article: Bosutinib mitigates inflammation in experimental sepsis
Bosutinib mitigates inflammation in experimental sepsis Open
Background Sepsis, a leading cause of death globally, lacks targeted and effective treatment. Its pathophysiology involves unbalanced inflammation, marked by a high release of inflammatory mediators, leukocyte recruitment, vascular changes…
View article: Mimicking the Liver Sinusoidal Endothelial Cell Niche In Vitro to Enhance Fenestration in a Genetic Model of Systemic Inflammation
Mimicking the Liver Sinusoidal Endothelial Cell Niche In Vitro to Enhance Fenestration in a Genetic Model of Systemic Inflammation Open
Liver sinusoidal endothelial cells (LSECs) play a crucial role in hepatic homeostasis, clearance, and microcirculatory regulation. Their fenestrations—patent transcellular pores—are essential for proper liver function, yet disappear in pat…
View article: Intravital imaging of neovascularization by two-photon laser scanning microscopy in tibial bone defects
Intravital imaging of neovascularization by two-photon laser scanning microscopy in tibial bone defects Open
Neovascularization plays a critical role in bone regeneration and skeletal development. Our understanding of weight-bearing bone healing has been hindered by the lack of a reliable method that allows tracking neovascularization at a high s…
View article: Intravital imaging of peritubular microcirculation impairment in cisplatin-induced acute kidney injury
Intravital imaging of peritubular microcirculation impairment in cisplatin-induced acute kidney injury Open
Despite the accumulation of cisplatin in proximal tubules, direct visualization of the surrounding peritubular microcirculation, including its change in cisplatin-induced acute kidney injury (AKI), is lacking. Here, using fluorescence and …