Phase imaging
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Adaptive dynamic range shift (ADRIFT) quantitative phase imaging Open
Quantitative phase imaging (QPI) with its high-contrast images of optical phase delay (OPD) maps is often used for label-free single-cell analysis. Contrary to other imaging methods, sensitivity improvement has not been intensively explore…
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Quantitative Phase Imaging: Recent Advances and Expanding Potential in Biomedicine Open
Quantitative phase imaging (QPI) is a label-free, wide-field microscopy approach with significant opportunities for biomedical applications. QPI uses the natural phase shift of light as it passes through a transparent object, such as a mam…
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Tomographic phase microscopy: principles and applications in bioimaging [Invited] Open
Tomographic phase microscopy (TPM) is an emerging optical microscopic technique for bioimaging. TPM uses digital holographic measurements of complex scattered fields to reconstruct three-dimensional refractive index (RI) maps of cells with…
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Quantitative phase imaging for medical diagnosis Open
Optical microscopy is an indispensable diagnostic tool in modern healthcare. As a prime example, pathologists rely exclusively on light microscopy to investigate tissue morphology in order to make a diagnosis. While advances in light micro…
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High-resolution and large field-of-view Fourier ptychographic microscopy and its applications in biomedicine Open
Fourier ptychographic microscopy (FPM) is a promising and fast-growing computational imaging technique with high resolution, wide field-of-view (FOV) and quantitative phase recovery, which effectively tackles the problems of phase loss, ab…
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Light sheet theta microscopy for rapid high-resolution imaging of large biological samples Open
The reported LSTM approach is a significant step for the rapid high-resolution quantitative mapping of the structure and function of very large biological systems, such as a clarified thick coronal slab of human brain and uniformly expande…
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Single-pixel digital holography with phase-encoded illumination Open
We demonstrate imaging of complex amplitude objects through digital holography with phase-structured illumination and bucket detection. The object is sampled with a set of micro-structured phase patterns implemented onto a liquid-crystal s…
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Characterising live cell behaviour: Traditional label-free and quantitative phase imaging approaches Open
Label-free imaging uses inherent contrast mechanisms within cells to create image contrast without introducing dyes/labels, which may confound results. Quantitative phase imaging is label-free and offers higher content and contrast compare…
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Quantitative phase microscopy spatial signatures of cancer cells Open
We present cytometric classification of live healthy and cancerous cells by using the spatial morphological and textural information found in the label‐free quantitative phase images of the cells. We compare both healthy cells to primary t…
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Resolution enhancement in quantitative phase microscopy Open
Quantitative phase microscopy (QPM), a technique combining phase imaging and microscopy, enables visualization of the 3D topography in reflective samples, as well as the inner structure or refractive index distribution of transparent and t…
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Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy Open
We present a simple and fast phase aberration compensation method in digital holographic microscopy (DHM) for quantitative phase imaging of living cells. By analyzing the frequency spectrum of an off-axis hologram, phase aberrations can be…
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Label-free biochemical quantitative phase imaging with mid-infrared photothermal effect Open
Label-free optical imaging is valuable in biology and medicine because of its non-destructive nature. Quantitative phase imaging (QPI) and molecular vibrational imaging (MVI) are the two most successful label-free methods, providing morpho…
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Epi-mode tomographic quantitative phase imaging in thick scattering samples Open
Quantitative phase imaging (QPI) is an important tool in biomedicine that allows for the microscopic investigation of live cells and other thin, transparent samples. Importantly, this technology yields access to the cellular and sub-cellul…
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Quantitative phase imaging for cell culture quality control Open
The potential of quantitative phase imaging (QPI) with digital holographic microscopy (DHM) for quantification of cell culture quality was explored. Label‐free QPI of detached single cells in suspension was performed by Michelson interfero…
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Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC) Open
We present a new technique for quantitative phase and amplitude microscopy from a single color image with coded illumination. Our system consists of a commercial brightfield microscope with one hardware modification-an inexpensive 3D print…
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Quantitative Phase Imaging Flow Cytometry for Ultra‐Large‐Scale Single‐Cell Biophysical Phenotyping Open
Cellular biophysical properties are the effective label‐free phenotypes indicative of differences in cell types, states, and functions. However, current biophysical phenotyping methods largely lack the throughput and specificity required i…
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Phase-resolved terahertz self-detection near-field microscopy Open
At terahertz (THz) frequencies, scattering-type scanning near-field optical microscopy (s-SNOM) based on continuous wave sources mostly relies on cryogenic and bulky detectors, which represents a major constraint for its practical applicat…
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All‐Optical Phase Recovery: Diffractive Computing for Quantitative Phase Imaging Open
Quantitative phase imaging (QPI) is a label‐free computational imaging technique that provides optical path length information of specimens. In modern implementations, the quantitative phase image of an object is reconstructed digitally th…
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Phase Retrieval: From Computational Imaging to Machine Learning: A tutorial Open
Phase retrieval consists in the recovery of a complex-valued signal from intensity-only measurements. As it pervades a broad variety of applications, many researchers have striven to develop phase-retrieval algorithms. Classical approaches…
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Real‐Time Stain‐Free Classification of Cancer Cells and Blood Cells Using Interferometric Phase Microscopy and Machine Learning Open
We present a method for real‐time visualization and automatic processing for detection and classification of untreated cancer cells in blood during stain‐free imaging flow cytometry using digital holographic microscopy and machine learning…
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Roadmap on Digital Holography-Based Quantitative Phase Imaging Open
Quantitative Phase Imaging (QPI) provides unique means for the imaging of biological or technical microstructures, merging beneficial features identified with microscopy, interferometry, holography, and numerical computations. This roadmap…
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3D-printed biological cell phantom for testing 3D quantitative phase imaging systems Open
As the 3D quantitative phase imaging (QPI) methods mature, their further development calls for reliable tools and methods to characterize and compare their metrological parameters. We use refractive index engineering during two-photon lase…
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Quantitative phase microscopy for cellular dynamics based on transport of intensity equation Open
We demonstrate a simple method for quantitative phase imaging of tiny transparent objects such as living cells based on the transport of intensity equation. The experiments are performed using an inverted bright field microscope upgraded w…
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Tutorial: Common path self-referencing digital holographic microscopy Open
Quantitative phase imaging of cells provides important morphological information about them, leading to their characterization, comparison, and identification. The interference principle when applied to microscopy provides high-contrast qu…
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Quantitative phase contrast imaging with a nonlocal angle-selective metasurface Open
Phase contrast microscopy has played a central role in the development of modern biology, geology, and nanotechnology. It can visualize the structure of translucent objects that remains hidden in regular optical microscopes. The optical la…
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Quantitative Phase Imaging for Label-Free Analysis of Cancer Cells—Focus on Digital Holographic Microscopy Open
To understand complex biological processes, scientists must gain insight into the function of individual living cells. In contrast to the imaging of fixed cells, where a single snapshot of the cell’s life is retrieved, live-cell imaging al…
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Ghost diffraction holographic microscopy Open
Unconventional systems that adopt the concept of ghost schemes have led to advancements in some imaging applications. However, their application in quantitative phase imaging remains a challenge. Here, we introduce a basis for quantitative…
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White-light quantitative phase imaging unit Open
We introduce the white-light quantitative phase imaging unit (WQPIU) as a practical realization of quantitative phase imaging (QPI) on standard microscope platforms. The WQPIU is a compact stand-alone unit which measures sample induced pha…
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Automated Fourier space region-recognition filtering for off-axis digital holographic microscopy Open
Automated label-free quantitative imaging of biological samples can greatly benefit high throughput diseases diagnosis. Digital holographic microscopy (DHM) is a powerful quantitative label-free imaging tool that retrieves structural detai…
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Scan-less hyperspectral dual-comb single-pixel-imaging in both amplitude and phase Open
We have developed a hyperspectral imaging scheme that involves a combination of dual-comb spectroscopy and Hadamard-transform-based single-pixel imaging. The scheme enables us to obtain 12,000 hyperspectral images of amplitude and phase at…