Photobleaching
View article: Near-infrared MINFLUX imaging enabled by suppression of fluorophore blinking
Near-infrared MINFLUX imaging enabled by suppression of fluorophore blinking Open
MINimal photon FLUXes (MINFLUX) offers nanometer localization precision, with lower fluorophore requirements than for other super-resolution microscopy (SRM) techniques. Nonetheless, low localization probabilities hamper its application, a…
View article: Dataset for three-dimensional lattice structured illumination microscopy
Dataset for three-dimensional lattice structured illumination microscopy Open
Structured illumination microscopy (SIM) is widely used for live-cell super-resolution imaging. Compared with the two-dimensional implementation (2D-SIM), the three-dimensional implementation (3D-SIM) provides twofold improvement in both l…
View article: Dataset for three-dimensional lattice structured illumination microscopy
Dataset for three-dimensional lattice structured illumination microscopy Open
Structured illumination microscopy (SIM) is widely used for live-cell super-resolution imaging. Compared with the two-dimensional implementation (2D-SIM), the three-dimensional implementation (3D-SIM) provides twofold improvement in both l…
View article: Fluorophores in the near-infrared region
Fluorophores in the near-infrared region Open
Fluorescence imaging in the near-infrared (NIR) and short-wave infrared (SW-IR) ranges has emerged as a promising area, providing wide-ranging possibilities for use in both biology and medicine. This spectral range is advantageous for biol…
View article: High content 3D Imaging by dual-view oblique plane microscopy
High content 3D Imaging by dual-view oblique plane microscopy Open
Oblique plane microscopy (OPM) is a form of light-sheet fluorescence microscopy (LSFM) employing a single microscope objective at the sample for both fluorescence excitation and detection. Dual-view OPM (dOPM) is an optically folded form o…
View article: Autocatalytic Circular DNA Powered Plasmonic CRISPR/Cas12a Platform for Ultrasensitive Non‐Nucleic Acid Target Sensing
Autocatalytic Circular DNA Powered Plasmonic CRISPR/Cas12a Platform for Ultrasensitive Non‐Nucleic Acid Target Sensing Open
CRISPR/Cas12a‐based detection of non‐nucleic acid targets faces two major challenges: 1) limited sensitivity due to the inherent inability to pre‐amplify non‐nucleic acid targets, and 2) suboptimal performance of traditional reporters caus…
View article: Study on the Airway Mucus Penetration Performance of Biodegradable Nanoparticles Based on Surface PEGylation in a Cystic Fibrosis Model
Study on the Airway Mucus Penetration Performance of Biodegradable Nanoparticles Based on Surface PEGylation in a Cystic Fibrosis Model Open
Cystic fibrosis (CF) airway mucus is thick and sticky, which makes it hard for nanoparticles to move and deliver drugs effectively. In this study, degradable PLGA nanoparticles were coated with polyethylene glycol (PEG) of different molecu…
View article: alyssadongqiliu/fluorescence-brightness-tracker: v0.1.0 - Initial Release
alyssadongqiliu/fluorescence-brightness-tracker: v0.1.0 - Initial Release Open
FluoTrack v0.1.0 - Initial Release First release of FluoTrack: Multi-target fluorescence tracker with Hungarian algorithm Features Multi-target detection: Adaptive spot detection using MAD-based thresholding Hungarian algorithm: Optimal tr…
View article: alyssadongqiliu/fluorescence-brightness-tracker: v0.1.0 - Initial Release
alyssadongqiliu/fluorescence-brightness-tracker: v0.1.0 - Initial Release Open
FluoTrack v0.1.0 - Initial Release First release of FluoTrack: Multi-target fluorescence tracker with Hungarian algorithm Features Multi-target detection: Adaptive spot detection using MAD-based thresholding Hungarian algorithm: Optimal tr…
View article: Understanding divergent Brown Carbon Photobleaching Rates from Molecular Perspective
Understanding divergent Brown Carbon Photobleaching Rates from Molecular Perspective Open
The global radiative effect of brown carbon (BrC) remains highly uncertain. BrC’s photobleaching, which significantly alerts its radiative effect, has been still poorly constrained. This study investigates photobleaching rates of laborator…
View article: Graded regulation of microtubule-binding of Tau by the phosphorylation state of the proline-rich region in living neurons
Graded regulation of microtubule-binding of Tau by the phosphorylation state of the proline-rich region in living neurons Open
Tau protein is a microtubule-associated protein that plays a crucial role in maintaining neuronal morphology and axonal transport. While phosphorylation is known to regulate Tau-microtubule interactions, the contribution of specific phosph…
View article: Poly(2‐Propyl‐2‐Oxazoline)‐Induced Lipid Nanotube Formation in Phospholipid Multilayers
Poly(2‐Propyl‐2‐Oxazoline)‐Induced Lipid Nanotube Formation in Phospholipid Multilayers Open
Synthetic amphiphilic polymers are promising regulators of biomembrane morphology because of their moderate hydrophobicity and tunable molecular properties, including higher‐order structure, charge, topology, and stimuli responsiveness. Ho…
View article: Protocol for autofluorescence removal in microglia by photobleaching in free-floating immunofluorescent staining of mouse brain tissue
Protocol for autofluorescence removal in microglia by photobleaching in free-floating immunofluorescent staining of mouse brain tissue Open
View article: Modeling Photobleaching and Absorption in Skin Tissue Using Nonlinear Optics: Applications in Laser Techniques and Dosimetry for Photodynamic Therapy
Modeling Photobleaching and Absorption in Skin Tissue Using Nonlinear Optics: Applications in Laser Techniques and Dosimetry for Photodynamic Therapy Open
Photodynamic therapy (PDT) efficacy arises from the interplay of photosensitizer photobleaching, evolving tissue optics, and nonlinear light–tissue interactions. Conventional dosimetry assumes exponential decay and constant optical propert…
View article: Green synthesis of silver nanoparticles from Centella asiatica and its application in photodegradation of methylene blue dye in paper industry effluent
Green synthesis of silver nanoparticles from Centella asiatica and its application in photodegradation of methylene blue dye in paper industry effluent Open
View article: Dataset for " All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins"
Dataset for " All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins" Open
Dataset that supports the observation, characterization and application of the paper entitled "All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins", where By mapping Reversibly Switchable Fluores…
View article: Light sheet microscopy and spectroscopy of whispering gallery mode microlaser particles
Light sheet microscopy and spectroscopy of whispering gallery mode microlaser particles Open
Whispering gallery mode microlasers are a recently developed sensing tool which can be used to make localised measurements of refractive index changes in a biological tissue through examination of their emission spectrum. This allows the d…
View article: Dataset for " All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins"
Dataset for " All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins" Open
Dataset that supports the observation, characterization and application of the paper entitled "All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins", where By mapping Reversibly Switchable Fluores…
View article: Continuous exchange of an inner-membrane ring component is required for assembly and function of the type III secretion system
Continuous exchange of an inner-membrane ring component is required for assembly and function of the type III secretion system Open
Major bacterial pathogens manipulate eukaryotic target cells by injecting effector proteins through type III secretion systems (T3SS). Recent in situ observations revealed that these large molecular machines, often referred to as injectiso…
View article: Galvanometer-scanning transient phase microscopy with balanced detection and arbitrary pump polarization
Galvanometer-scanning transient phase microscopy with balanced detection and arbitrary pump polarization Open
Transient absorption microscopy measures excited-state kinetics based on the imaginary part of the pump-induced perturbation to the complex refractive index, i.e. $\Im \{Δ\mathcal N\}$, with applications in both materials and biomedical sc…
View article: Galvanometer-scanning transient phase microscopy with balanced detection and arbitrary pump polarization
Galvanometer-scanning transient phase microscopy with balanced detection and arbitrary pump polarization Open
Transient absorption microscopy measures excited-state kinetics based on the imaginary part of the pump-induced perturbation to the complex refractive index, i.e. $\Im \{Δ\mathcal N\}$, with applications in both materials and biomedical sc…
View article: Modulating Photodissociation and Photobleaching via Plasmon Resonance to Enhance Light-Induced Nitric Oxide Release
Modulating Photodissociation and Photobleaching via Plasmon Resonance to Enhance Light-Induced Nitric Oxide Release Open
Localized therapeutic action and targeted drug release offer compelling advantages over traditional systemic drug administration. This is particularly important for nitric oxide (NO), whose biological effects vary greatly depending on conc…
View article: SlimVar for rapid in vivo single-molecule tracking of chromatin regulators in plants
SlimVar for rapid in vivo single-molecule tracking of chromatin regulators in plants Open
Epigenetic regulation occurs over many rounds of cell division in higher organisms. However, visualisation of the regulators in vivo is limited by imaging dynamic molecules deep in tissue. We report a technology—Variable-angle Slimfield mi…
View article: ARHGAP4/MYH9/β-catenin/c-Jun positive feedback loop promotes colorectal cancer stemness
ARHGAP4/MYH9/β-catenin/c-Jun positive feedback loop promotes colorectal cancer stemness Open
View article: Miniaturized Single‐Photon Level Computational Complex Field Imaging System via Meta‐Optics
Miniaturized Single‐Photon Level Computational Complex Field Imaging System via Meta‐Optics Open
Compact optical systems are revolutionizing precision measurement with their miniaturized design and functionality. At the forefront of this revolution are metalenses, composed of sub‐wavelength nanostructures. They have emerged as novel p…
View article: Label-Free Longitudinal Imaging of Single Cell Drug Response with a 3D-Printed Cell Culture Platform
Label-Free Longitudinal Imaging of Single Cell Drug Response with a 3D-Printed Cell Culture Platform Open
Image-based phenotypic screening has emerged as a powerful tool for revealing single-cell heterogeneity and dynamic phenotypic responses in preclinical drug discovery. Compared to traditional static end-point assays, live-cell longitudinal…
View article: Author Correction: Impact of photobleaching on quantitative, spatio-temporal, super-resolution imaging of mitochondria in live C. elegans larvae
Author Correction: Impact of photobleaching on quantitative, spatio-temporal, super-resolution imaging of mitochondria in live C. elegans larvae Open
View article: Environmentally Friendly Photobleaching Method Using Visible Light for Removing Natural Stains from Clothing
Environmentally Friendly Photobleaching Method Using Visible Light for Removing Natural Stains from Clothing Open
View article: Photothermal and Photodynamic Strategies for Diagnosis and Therapy of Alzheimer’s Disease by Modulating Amyloid-β Aggregation
Photothermal and Photodynamic Strategies for Diagnosis and Therapy of Alzheimer’s Disease by Modulating Amyloid-β Aggregation Open
Amyloid-β (Aβ) aggregates are considered as the important factors of Alzheimer’s disease (AD). Multifunctional materials have shown significant effects in the diagnosis and treatment of AD by modulating the aggregation of Aβ and production…
View article: Fluorescence recovery after photobleaching reveals different behaviour of tropomyosin isoforms Tpm3.1 and Tpm4.2 in dendritic spines
Fluorescence recovery after photobleaching reveals different behaviour of tropomyosin isoforms Tpm3.1 and Tpm4.2 in dendritic spines Open
Actin is the predominant cytoskeletal structure in both the pre- and the post-synaptic compartment of excitatory synapses in the brain, which are formed between the distal part of the axon and the distal site of dendritic spines. Tropomyos…