Microdialysis
View article: Lipid Composition and Thermotropic Properties of Meibum of Animal Models and Humans with Meibomian Gland Dysfunction
Lipid Composition and Thermotropic Properties of Meibum of Animal Models and Humans with Meibomian Gland Dysfunction Open
Meibum—a lipid-rich secretion produced by holocrine Meibomian glands (MG)—plays a central role in maintaining ocular surface homeostasis in humans. Previously, changes in MG lipidomes induced by inactivation of critical genes of meibogenes…
View article: Sex‐specific impact of repeated adolescent vapour exposure to JWH‐018 on dopamine response, behaviour and pharmacokinetics across adolescence and adulthood
Sex‐specific impact of repeated adolescent vapour exposure to JWH‐018 on dopamine response, behaviour and pharmacokinetics across adolescence and adulthood Open
Background and Purpose Alarming trends show that vaping e‐cigarettes containing synthetic cannabinoid receptor agonists, such as JWH‐018, is increasing among youth. However, the effects of these trends are unclear in both sexes. We therefo…
View article: Preclinical pharmacokinetic characterization of (R)-ketamine injection, a novel antidepressant glutamatergic agent
Preclinical pharmacokinetic characterization of (R)-ketamine injection, a novel antidepressant glutamatergic agent Open
Introduction Ketamine is a significant class of antidepressant drugs, and the pharmacokinetic and pharmacodynamic characteristics of its enantiomers exhibit differences. Among them, intravenous infusion of (R)-ketamine can effectively alle…
View article: Relation Between Inflammatory Parameters and Insulin Resistance Indices in Cows During Early Lactation
Relation Between Inflammatory Parameters and Insulin Resistance Indices in Cows During Early Lactation Open
Background/Objectives: Early lactation in high-producing dairy cows is a critical period characterized by pronounced negative energy balance, enhanced lipomobilization, and the development of insulin resistance (IR), often accompanied by l…
View article: IFNγ-associated immune–metabolic remodeling drives serotonin–kynurenine imbalance with cortical vulnerability in lupus-prone mice
IFNγ-associated immune–metabolic remodeling drives serotonin–kynurenine imbalance with cortical vulnerability in lupus-prone mice Open
Introduction Neuropsychiatric systemic lupus erythematosus (NPSLE) is a major clinical challenge, characterized by heterogeneous manifestations and the absence of reliable biomarkers. The mechanisms linking systemic autoimmunity to neurona…
View article: Pro-Cognitive Effect of Royal Jelly Is Linked with Increased Burst Activity of Mesocorticolimbic Dopaminergic Neurons
Pro-Cognitive Effect of Royal Jelly Is Linked with Increased Burst Activity of Mesocorticolimbic Dopaminergic Neurons Open
Background: Royal jelly is a protein-rich honeybee secretion that is used in the nutrition of larvae and adult queens. Previous studies have reported that royal jelly had induced pro-cognitive, anxiolytic, and antidepressant-like effects i…
View article: Protective effects of liver-derived apolipoprotein A1 against heat stress-induced hypothalamic lipid metabolism and blood-brain barrier integrity
Protective effects of liver-derived apolipoprotein A1 against heat stress-induced hypothalamic lipid metabolism and blood-brain barrier integrity Open
Heat stress (HS), a prevalent occupational and environmental hazard, has increasingly been recognized as a major contributor to multiple physiological disorders. The hypothalamus, a key regulator of thermoregulation and endocrine signaling…
View article: Pharmacodynamic effects and plasma pharmacokinetics of N, N-dimethyltryptamine after intranasal versus subcutaneous administration in male rats
Pharmacodynamic effects and plasma pharmacokinetics of N, N-dimethyltryptamine after intranasal versus subcutaneous administration in male rats Open
Rationale There is growing interest in the therapeutic utility of psychedelic compounds that act as serotonin-2 A receptor (5-HT 2A ) agonists. N , N -dimethyltryptamine (DMT) is a 5-HT 2A agonist that produces intense and short-lived psyc…
View article: Elevated glioma-related cortical glutamate <i>in vivo</i> is associated with <i>ex vivo</i> interictal discharges in human brain slices
Elevated glioma-related cortical glutamate <i>in vivo</i> is associated with <i>ex vivo</i> interictal discharges in human brain slices Open
Gliomas are the most common type of malignant brain tumour and are frequently associated with seizures. Seizures associated with gliomas are frequently refractory to conventional antiseizure medications and constitute a significant source …
View article: Losing the Filter: How Kynurenine Pathway Dysregulation Impairs Habituation
Losing the Filter: How Kynurenine Pathway Dysregulation Impairs Habituation Open
Habituation is a fundamental form of non-associative learning that allows organisms to filter out repetitive, non-salient stimuli but declines with age. While the kynurenine pathway (KP) of tryptophan metabolism is implicated in psychiatri…
View article: Catecholaminergic Adaptation to Extreme Military Stress: Norepinephrine and Dopamine Responses During and After SERE Training
Catecholaminergic Adaptation to Extreme Military Stress: Norepinephrine and Dopamine Responses During and After SERE Training Open
Catecholamines are fundamental mediators of the stress response, regulating arousal, vigilance, and adaptive behavior. However, their dynamics under extreme real-life conditions remain insufficiently explored. Survival, Evasion, Resistance…
View article: Measurement of Glutamate Suppression in a 6-OHDA-Induced Dopamine Deficiency Rat Model Following Acute Single-Dose L-DOPA Using GluCEST/MRS
Measurement of Glutamate Suppression in a 6-OHDA-Induced Dopamine Deficiency Rat Model Following Acute Single-Dose L-DOPA Using GluCEST/MRS Open
Background/Objectives: The Glutamate Chemical Exchange Saturation Transfer (GluCEST) technique is an advanced imaging modality that enables non-invasive glutamate quantification using MRI. Methods: This study evaluated glutamate dynamics i…
View article: Metabolic and Ischemic Stress Synergistically Drive Time-Dependent Progression of Vasculogenic Erectile Dysfunction in a Rat Model
Metabolic and Ischemic Stress Synergistically Drive Time-Dependent Progression of Vasculogenic Erectile Dysfunction in a Rat Model Open
Background Erectile dysfunction (ED) is a common male sexual disorder, with the vasculogenic subtype primarily driven by endothelial injury and metabolic dysfunction. Current research often lacks a systematic, time-course analysis of its p…
View article: Exercise-related physiological state classification via a pattern-driven flexible impedimetric e-tongue using holistic sweat analysis
Exercise-related physiological state classification via a pattern-driven flexible impedimetric e-tongue using holistic sweat analysis Open
Sweat analysis has significant potential in personalized health monitoring and exercise physiology assessment due to its non-invasive and real-time nature. However, the complexity of sweat composition limits the applicability of existing p…
View article: Magnetic resonance imaging determination of tissue sodium across the CKD spectrum – associations and implications for health
Magnetic resonance imaging determination of tissue sodium across the CKD spectrum – associations and implications for health Open
Background and Hypothesis Increased tissue sodium (Na+) concentration is associated with adverse clinical outcomes in people with chronic kidney disease (CKD) including hypertension, inflammation and increased cardiovascular disease (CVD).…
View article: The effect of the nonselective TNF inhibitor etanercept, and of the selective TNF inhibitor XPro1595 on lesioned supraspinatus muscle
The effect of the nonselective TNF inhibitor etanercept, and of the selective TNF inhibitor XPro1595 on lesioned supraspinatus muscle Open
The cytokine tumor necrosis factor (TNF), a major regulator of inflammatory responses, exists in both a membrane bound form and a soluble form. We used the nonselective TNF inhibitor etanercept, and the selective inhibitor XPro1595 and com…
View article: Impact of regional and general aerobic exercise on molecular regulators oflipolysis and adipose tissue composition in obese women
Impact of regional and general aerobic exercise on molecular regulators oflipolysis and adipose tissue composition in obese women Open
This randomized controlled trial compared the effects of regional, general, and combined aerobic training on plasma lipolytic markers and subcutaneous fat reduction in obese women with abdominal or gluteofemoral fat accumulation. Sixty wom…
View article: Modulatory Effects of Jujuboside A on Amino Acid Neurotransmitter Profiles in Tic Disorder
Modulatory Effects of Jujuboside A on Amino Acid Neurotransmitter Profiles in Tic Disorder Open
Objective This study investigates the impact of jujuboside A (JuA) on the levels of glutamate (Glu) and gamma‐aminobutyric acid (GABA) in the extracellular fluid of the caudate putamen (CPu) in a rat model of tic disorder (TD). It also com…
View article: Development and Validation of a High Performance Liquid Chromatography Method for the Determination of Bromopride in the Presence of Methylparaben and Propylparaben in Pharmaceutical Formulations
Development and Validation of a High Performance Liquid Chromatography Method for the Determination of Bromopride in the Presence of Methylparaben and Propylparaben in Pharmaceutical Formulations Open
A reversed‐phase high‐performance liquid chromatography method was developed and validated for the determination of bromopride (BROP) in the presence of methylparaben and propylparaben, using isocratic mode, C18 chromatographic column, mob…
View article: BIOM-106. MICROPERFUSION-BASED BIOMARKER ANALYSIS IN GLIOBLASTOMA: INTEGRATING PRECLINICAL AND CLINICAL PERSPECTIVES
BIOM-106. MICROPERFUSION-BASED BIOMARKER ANALYSIS IN GLIOBLASTOMA: INTEGRATING PRECLINICAL AND CLINICAL PERSPECTIVES Open
Glioblastomas (GBMs) are aggressive brain tumors that pose therapeutic challenges. Microperfusion (MP) probes offer a unique method for real-time sampling of extracellular biomarkers and drug responses within the tumor microenvironment. Co…
View article: EXTH-32. Preclinical evaluation of the CNS penetration of a novel series of MTA-cooperative PRMT5 inhibitors including TNG456
EXTH-32. Preclinical evaluation of the CNS penetration of a novel series of MTA-cooperative PRMT5 inhibitors including TNG456 Open
The treatment of central nerve system (CNS) malignancies, including metastases, remains a significant unmet medical need. This is especially true for glioblastoma, where up to 50% of cases have loss of MTAP, which creates a dependency on P…
View article: Human Adipose Tissue 11β‐Hydroxysteroid Dehydrogenase Type 1 Inhibition Without Tachyphylaxis by Clofutriben, a Pseudo‐Irreversible Enzyme Inhibitor
Human Adipose Tissue 11β‐Hydroxysteroid Dehydrogenase Type 1 Inhibition Without Tachyphylaxis by Clofutriben, a Pseudo‐Irreversible Enzyme Inhibitor Open
Inhibitors of 11β‐hydroxysteroid dehydrogenase type 1 (HSD‐1), the pre‐receptor regulator of intracellular active glucocorticoid levels, have promise to treat glucocorticoid excess whether endogenous (neoplastic or physiological elevated c…
View article: Biomarker Analysis of Mechanically Ventilated Adults using Propofol or Dexmedetomidine
Biomarker Analysis of Mechanically Ventilated Adults using Propofol or Dexmedetomidine Open
BACKGROUND: Increased inflammatory cytokines and biomarkers have been related to the degradation of the blood brain barrier. The fragility of this barrier can increase the number of proteins that can enter the central nervous system circul…
View article: Defining metabolic abnormalities in acute human traumatic brain injury with cerebral microdialysis and multimodality monitoring
Defining metabolic abnormalities in acute human traumatic brain injury with cerebral microdialysis and multimodality monitoring Open
Objective We aimed to compare the prevalence and multimodal associations of mitochondrial dysfunction as defined by published cerebral-microdialysis-based criteria versus our novel multimodality-monitoring-based criteria in acute traumatic…
View article: Cerebral microdialysis – mechanism of action and application in clinical practice
Cerebral microdialysis – mechanism of action and application in clinical practice Open
Cerebral microdialysis enables bedside assessment of the concentration of selected metabolites in the patient’s brain. The modern microdialysis device consists of a double-lumen probe. Its interior is made of an impermeable material, while…
View article: CarboCell G/C offers high and prolonged concentrations of gentamicin and clindamycin in bone tissue following intraosseous injection
CarboCell G/C offers high and prolonged concentrations of gentamicin and clindamycin in bone tissue following intraosseous injection Open
Objectives: The novel local antibiotic-eluting depot technology CarboCell G/C has shown great promise in terms of efficacy in an osteomyelitis pig model. The present study aimed to investigate the initial release kinetics of gentamicin and…
View article: Development of a Novel Immunoprecipitation Method for Extracting Monoclonal Antibodies From Brain Tissue and Its Application to Assessing In Vivo Brain Penetration in Mouse via Liquid Chromatography–Mass Spectrometry
Development of a Novel Immunoprecipitation Method for Extracting Monoclonal Antibodies From Brain Tissue and Its Application to Assessing In Vivo Brain Penetration in Mouse via Liquid Chromatography–Mass Spectrometry Open
A novel extraction method was developed to quantify monoclonal antibodies from brain tissue, crucial for pharmacokinetic assessments. We focused on optimizing detergent use for compatibility with liquid chromatography–mass spectrometry ana…
View article: Neuropeptides in the Extracellular Space of the Mouse Cortex Measured In Vivo by Nanodialysis Probe Coupled with LC‐MS
Neuropeptides in the Extracellular Space of the Mouse Cortex Measured In Vivo by Nanodialysis Probe Coupled with LC‐MS Open
Neuropeptides are key neuromodulators in the central nervous system that shape sensory processing, yet their extracellular dynamics in the somatosensory cortex (S1) remain poorly understood. This study applies an innovative membrane‐free s…
View article: Neuropeptides in the Extracellular Space of the Mouse Cortex Measured In Vivo by Nanodialysis Probe Coupled with LC‐MS
Neuropeptides in the Extracellular Space of the Mouse Cortex Measured In Vivo by Nanodialysis Probe Coupled with LC‐MS Open
Neuropeptides are key neuromodulators in the central nervous system that shape sensory processing, yet their extracellular dynamics in the somatosensory cortex (S1) remain poorly understood. This study applies an innovative membrane‐free s…
View article: Abstinence from cocaine self-administration promotes microglial pruning of astrocytes, which drives cocaine-seeking behavior
Abstinence from cocaine self-administration promotes microglial pruning of astrocytes, which drives cocaine-seeking behavior Open
Rodent drug self-administration leads to a compromised ability of nucleus accumbens astrocytes to maintain glutamate homeostasis as well as to reductions in surface area, volume, and synaptic colocalization of astrocyte membranes. However,…