Scaffold protein
View article: Figure S3 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling
Figure S3 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling Open
Dose response of PK-59 cells transfected with scramble siRNA and indicated candidate gene siRNAs; and CNKSR1 immunohistochemical staining in pancreas cancer tissues.
View article: Figure S5 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling
Figure S5 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling Open
Selumetinib fails to recruit CNKSR1 and p-AKT to the plasma membrane in CNKSR1 low cell lines; Proximal ligation of CNKSR1 and p-AKT in CNKSR1 low cell lines treated with vehicle vs selumetinib;CNKSR1 p-AKT PLA signal after treatment with …
View article: Figure S6 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling
Figure S6 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling Open
MEK blockade fails to disrupt protein-protein interactions of CNKSR1, KSR1/2, and MEK in CNKSR1low cancer cells.
View article: Table S1 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling
Table S1 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling Open
Lists of cell lines, antibodies, RNAi kinome screen; and matrix drug combination.
View article: Figure S2 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling
Figure S2 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling Open
Secondary validation of candidate genes.
View article: Figure S1 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling
Figure S1 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling Open
Resistance to MEK inhibition in YAPC cells is not due to reactivation of phospho-Extracellular-signal-regulated-kinases (ERK) levels.
View article: Figure S7 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling
Figure S7 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling Open
Schematic layout of the selumetinib versus 26 compounds targeting effector signaling of CNKSR1 screen; and Dose response of MK-2206 and rapamycin in the pancreas cancer cell lines.
View article: Figure S4 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling
Figure S4 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling Open
CNKSR1 promotes MEK inhibition-induced AKT activation.
View article: A de novo missense variant in MIDEAS results in increased deacetylase activity of the MiDAC HDAC complex causing a neurodevelopmental syndrome
A de novo missense variant in MIDEAS results in increased deacetylase activity of the MiDAC HDAC complex causing a neurodevelopmental syndrome Open
MIDEAS is a scaffold protein that, together with DNTTIP1, mediates assembly of the MiDAC histone deacetylase complex. Mice lacking MiDAC die before birth suggesting a key developmental function. Here, we report two unrelated individuals, w…
View article: Sh3rf3 Deficiency drives autism-like behaviors via presynaptic dysfunction in mice
Sh3rf3 Deficiency drives autism-like behaviors via presynaptic dysfunction in mice Open
Autism spectrum disorder (ASD) is a prevalent and complex neurodevelopmental disorder with a strong genetic basis. Although SH3RF3 has been identified as an ASD candidate gene, its biological function and pathophysiological mechanisms rema…
View article: Induced Proximity Approach Enables the Recombinant Production of Polyphosphorylated Silk Proteins with Improved Adhesiveness
Induced Proximity Approach Enables the Recombinant Production of Polyphosphorylated Silk Proteins with Improved Adhesiveness Open
Phosphorylation is considered to play a role in many of the functional properties of silk proteins, affecting their solubility, environmental adaptability, adhesion, and biocompatibility. However, investigating these effects has been hampe…
View article: A novel targeting domain directs essential components of the cytosolic iron–sulfur cluster assembly pathway to the mitochondrion of Toxoplasma parasites
A novel targeting domain directs essential components of the cytosolic iron–sulfur cluster assembly pathway to the mitochondrion of Toxoplasma parasites Open
The assembly of iron–sulfur (FeS) clusters for cytosolic and nuclear proteins is essential for eukaryotic cell biology. This assembly is mediated by the Cytosolic Iron–sulfur Assembly (CIA) pathway, which localizes to the cytosol of most e…
View article: Towards effective RIHT therapies: Identifying potential biomarkers for in vitro thyroid scaffold assessment
Towards effective RIHT therapies: Identifying potential biomarkers for in vitro thyroid scaffold assessment Open
Radiation-induced hypothyroidism (RIHT) affects up to 92% of head and neck cancer patients post-irradiation, greatly impairing quality of life. Current treatments have limitations regarding efficacy and drug interactions. Tissue engineerin…
View article: Towards effective RIHT therapies: Identifying potential biomarkers for in vitro thyroid scaffold assessment
Towards effective RIHT therapies: Identifying potential biomarkers for in vitro thyroid scaffold assessment Open
Radiation-induced hypothyroidism (RIHT) affects up to 92% of head and neck cancer patients post-irradiation, greatly impairing quality of life. Current treatments have limitations regarding efficacy and drug interactions. Tissue engineerin…
View article: Extracellular Matrix Remodeling in Motor Neuron Diseases
Extracellular Matrix Remodeling in Motor Neuron Diseases Open
The extracellular matrix (ECM) constitutes a dynamic scaffold composed of both cellular and non-cellular elements that not only ensure tissue integrity but also regulate signaling events crucial for development and homeostasis. While its d…
View article: Molecular mechanisms shaping the actin filament scaffold of dendritic filopodia
Molecular mechanisms shaping the actin filament scaffold of dendritic filopodia Open
Dendritic spines are the postsynaptic compartment of most excitatory synapses in the vertebrate brain. Their morphology is defined by a complex actin scaffold consisting of branched and unbranched actin filaments (F-actin), which constitut…
View article: Longitudinal characterization of inflammatory plasma protein signatures in ECT response
Longitudinal characterization of inflammatory plasma protein signatures in ECT response Open
Background Electroconvulsive therapy (ECT) is an effective treatment for psychiatric disorders, but its molecular mechanisms are not yet fully elucidated. This study aimed to investigate longitudinal dynamics of inflammatory proteins durin…
View article: Structural insights into scaffold-guided assembly of the Pseudomonas phage D3 capsid
Structural insights into scaffold-guided assembly of the Pseudomonas phage D3 capsid Open
Tailed bacteriophages comprise the largest structural family of viruses with close relatives in archaea and the eukaryotic herpesviruses. The common assembly pathway produces an icosahedrally symmetric protein shell, called capsid, into wh…
View article: 6,7-Dihydroxy-4-methylcoumarin Suppresses Adipogenesis via AMPK and MAPK Signaling with In Silico Analysis of Adipogenic Proteins
6,7-Dihydroxy-4-methylcoumarin Suppresses Adipogenesis via AMPK and MAPK Signaling with In Silico Analysis of Adipogenic Proteins Open
Background/Objectives: Coumarin-based compounds exhibit diverse pharmacological properties, and 4-methylcoumarin (4MC) has emerged as a promising scaffold for drug development. However, its anti-obesity mechanisms remain insufficiently und…
View article: Autophagy and mitophagy at the synapse and beyond: implications for learning, memory and neurological disorders
Autophagy and mitophagy at the synapse and beyond: implications for learning, memory and neurological disorders Open
The human brain is one of the most metabolically active tissues in the body, due in large part to the activity of trillions of synaptic connections. Under normal conditions, macroautophagy/autophagy at the synapse plays a crucial role in s…
View article: A ubiquitin-like protein controls assembly of a bacterial type VIIb secretion system
A ubiquitin-like protein controls assembly of a bacterial type VIIb secretion system Open
Type VII secretion systems (T7SS) are protein translocation machines crucial for virulence and bacterial competition in Gram-positive bacteria. Despite their importance, the structural basis for assembly of type VIIb secretion systems (T7S…
View article: A Guide to Guides: An Overview of SpCas9 sgRNA Scaffold Variants and Modifications
A Guide to Guides: An Overview of SpCas9 sgRNA Scaffold Variants and Modifications Open
The CRISPR/SpCas9 system has revolutionized biology by enabling precise and programmable genome modification. While substantial effort has focused on engineering the SpCas9 protein and spacer sequences, the single-guide RNA (sgRNA) scaffol…
View article: Clathrin adaptors drive phase separation in endocytosis and trafficking
Clathrin adaptors drive phase separation in endocytosis and trafficking Open
Liquid–liquid phase separation (LLPS) underlies the formation of biomolecular condensates that organize cellular processes, including endocytosis and membrane trafficking. Although LLPS has been implicated in clathrin-mediated pathways, th…
View article: Adaptation of the endplate in skeletal muscle of Homer 2 <sup>-/-</sup> mice
Adaptation of the endplate in skeletal muscle of Homer 2 <sup>-/-</sup> mice Open
At the neuromuscular junction, nicotinic acetylcholine receptor (nAChR) dynamics is regulated in a nerve- and activity-dependent manner. Correlated local alterations in myoplasmic [Ca 2+ ] i , induced by IP 3 -sensitive subsynaptic Ca 2+ s…
View article: Brain-Derived Neurotrophic Factor Trafficking via the Regulated Secretory Pathway: Mechanisms and Disease Implications
Brain-Derived Neurotrophic Factor Trafficking via the Regulated Secretory Pathway: Mechanisms and Disease Implications Open
Brain-derived neurotrophic factor (BDNF) plays an essential role in neuronal development and synaptic plasticity. BDNF’s role in neuronal function is dependent on its trafficking through the regulated secretory pathway (RSP). This review p…
View article: <b>A Mechano-Feedback Loop Orchestrated by SUN1/2 Governs Cellular Mechanoadaptation via Lamina-Associated Domain Remodeling</b><b></b>
<b>A Mechano-Feedback Loop Orchestrated by SUN1/2 Governs Cellular Mechanoadaptation via Lamina-Associated Domain Remodeling</b><b></b> Open
SUN1/2, core components of the linker of nucleoskeleton and cytoskeleton (LINC) complex, transmit extracellular mechanical forces to nuclear lamina and chromatin. However, their role in regulating peripheral chromatin in mechanosensing and…
View article: Stability Analysis of Basic Load-Bearing Units in Independent Scaffolding Systems
Stability Analysis of Basic Load-Bearing Units in Independent Scaffolding Systems Open
Scaffolds, as temporary structural support systems in civil engineering, play an essential role during construction. Independent steel scaffold systems, typically composed of assembled steel tubes, can be erected and function as standalone…
View article: The <i>Shigella</i> siphophage Sf11 tail structure and host attachment mechanism
The <i>Shigella</i> siphophage Sf11 tail structure and host attachment mechanism Open
A paucity of reports is available describing the structures of Shigella phages, and these have focused to date on a few short, non-contractile podophages and one long contractile myophage. Here, we report the cryo-EM structure of a Shigell…
View article: Role of tankyrase scaffolding in the β-catenin destruction complex and WNT signaling
Role of tankyrase scaffolding in the β-catenin destruction complex and WNT signaling Open
Aberrant WNT/β-catenin signaling drives tumorigenesis and metastasis in cancer. Although enzymatic inhibitors of tankyrase (TNKS) effectively block AXIN degradation and stabilize the β-catenin destruction complex (DC), they have demonstrat…
View article: eLife Assessment: Role of tankyrase scaffolding in the β-catenin destruction complex and WNT signaling
eLife Assessment: Role of tankyrase scaffolding in the β-catenin destruction complex and WNT signaling Open