Ethanol precipitation
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Comparison of capture and storage methods for aqueous macrobial <span>eDNA</span> using an optimized extraction protocol: advantage of enclosed filter Open
Summary Aqueous environmental DNA ( eDNA ) is an emerging efficient non‐invasive tool for species inventory studies. To maximize performance of downstream quantitative PCR (qPCR) and next‐generation sequencing ( NGS ) applications, quality…
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Ultrasound irradiation alters the spatial structure and improves the antioxidant activity of the yellow tea polysaccharide Open
In this study, the impact of ultrasound irradiation on the structural characteristics and antioxidant properties of yellow tea polysaccharides with different molecular weights (Mw) were investigated. Native yellow tea polysaccharide contai…
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Extraction, Structure and Immunoregulatory Activity of Low Molecular Weight Polysaccharide from Dendrobium officinale Open
The ethanol precipitation method has been widely-used for Dendrobium officinale polysaccharides preparation. However, the alcohol-soluble fractions have always been ignored, which causes significant wastes of resources and energies. In thi…
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Physicochemical and Biological Characterization of Fucoidan from Fucus vesiculosus Purified by Dye Affinity Chromatography Open
A comparative study concerning the physicochemical, monomeric composition and biological characters among different fucoidan fractions is presented. Common purification techniques for fucoidan usually involve many steps. During these steps…
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Evaluation of the Efficiency of Ethanol Precipitation and Ultrafiltration on the Purification and Characteristics of Exopolysaccharides Produced by Three Lactic Acid Bacteria Open
Exopolysaccharides (EPS) produced by three Lactic Acid Bacteria strains, Lactococcus lactis SLT10, Lactobacillus plantarum C7, and Leuconostoc mesenteroides B3, were isolated using two methods: ethanol precipitation (EPS-ETOH) and ultrafil…
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Precipitation of RNA with Ethanol Open
Purified RNA may need to be concentrated by precipitation for downstream applications. Precipitation of RNA with ethanol (or isopropanol) is the standard method to recover RNA from aqueous solutions.
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Antioxidant, Anti-Tumour, and Anticoagulant Activities of Polysaccharide from Calocybe indica (APK2) Open
The initial structural features and in vitro biological study of crude polysaccharides from Calocybe indica (CICP) extracted by hot water followed by ethanol precipitation was investigated. High-performance gel permeation chromatography, H…
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Physical and Chemical Properties of Acacia mangium Lignin Isolated from Pulp Mill Byproduct for Potential Application in Wood Composites Open
The efficient isolation process and understanding of lignin properties are essential to determine key features and insights for more effective lignin valorization as a renewable feedstock for the production of bio-based chemicals including…
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Guideline for the extraction, isolation, purification, and structural characterization of polysaccharides from natural resources Open
Polysaccharide is widely distributed in natural resources, and currently attracts scientists' attention for their various bio‐functions including immunomodulatory activity, hypoglycemic activity, hypolipidemic activity, antitumor activity,…
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Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.) Open
A novel neutral polysaccharide designated as PAP1b was isolated from Areca catechu L. by hot water extraction, ethanol precipitation, and column chromatography. PAP1b was mainly composed of mannose, galactose, xylose, and arabinose in a ra…
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Characterization and Antioxidant Activity of the Exopolysaccharide Produced by Bacillus amyloliquefaciens GSBa-1 Open
The exopolysaccharide (EPS) produced by Bacillus amyloliquefaciens GSBa-1 was isolated and purified by ethanol precipitation, and DEAE-cellulose and Sepharose CL-6B chromatographies. The molecular mass of the purified EPS was determined to…
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Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation Open
Supplementary data related to this article can be found at https:// doi.org/10.1016/j.foodhyd.2018.09.009.
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Research progress on the ethanol precipitation process of traditional Chinese medicine Open
Ethanol precipitation is a purification process widely used in the purification of Chinese medicine concentrates. This article reviews the research progress on the process mechanism of ethanol precipitation, ethanol precipitation process a…
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Characterization of polysaccharide fractions from Allii macrostemonis bulbus and assessment of their antioxidant Open
In order to comprehensive understanding of the active substances of Allii macrostemonis bulbus (AMB), this study conducted the extraction, purification, structure and antioxidant activity of AMB polysaccharides. The crude polysaccharides w…
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Purification of Tea saponins and Evaluation of its Effect on Alcohol Dehydrogenase Activity Open
Tea saponins, extracted from a Camellia oleifera cake, were found to have a potent effect on de-alcoholic activity. To obtain highly pure tea saponins, which can better maintain the activity of alcohol dehydrogenase (ADH), this paper prese…
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Extraction, fractionation, and chemical characterisation of fucoidans from the brown seaweed Sargassum pallidum Open
The fucoidans were obtained by extraction with water and gradient precipitation with different concentrations of ethanol. The main structural characterisations and bioactivities were achieved. Four fractions of water-soluble crude fucoidan…
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Physicochemical features and antioxidant activity of polysaccharides from Herba Patriniae by gradient ethanol precipitation Open
Four polysaccharides BJP50, BJP60, BJP70 and BJP80 (total named BJPs) were separated from Herba Patriniae via gradient ethanol precipitation with 50%, 60%, 70% and 80%, respectively. After decolorization and deproteinization, their physico…
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Isopropanol precipitation method for collecting fish environmental DNA Open
The environmental DNA (eDNA) method has been used to estimate the distributions of aquatic species, and both ethanol precipitation and filtration‐based methods are commonly employed to capture eDNA from sampled water. Although filtration‐b…
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Optimization of Enzyme−Assisted Aqueous Extraction of Polysaccharide from Acanthopanax senticosus and Comparison of Physicochemical Properties and Bioactivities of Polysaccharides with Different Molecular Weights Open
To obtain the optimal process for the enzyme−assisted aqueous extraction of polysaccharides from Acanthopanax senticosus, and study the physicochemical properties of polysaccharides of different molecular weights, the extraction of Acantho…
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Characterization of Structural and Physicochemical Properties of an Exopolysaccharide Produced by Enterococcus sp. F2 From Fermented Soya Beans Open
The present study sought to isolate a novel exopolysaccharide (EPS-F2) from Enterococcus sp. F2 through ethanol precipitation, anion-exchange, and gel-filtration chromatography and characterize the physicochemical properties by spectral te…
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Characterization and Rheological Behavior of Dextran from<i> Weissella confusa</i> R003 Open
Dextran from Weissella confusa R003 isolated from sugar cane juice was purified and characterized. Dextran synthesis was performed by fermenting W. confusa R003 in MRS medium containing 10% (w/v) sucrose with continuous shaking at 125 rpm …
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Molecular characterization of polysaccharides in hot-water extracts of Ganoderma lucidum fruiting bodies Open
Polysaccharide components in hot-water extract of Ganoderma lucidum fruiting bodies were separated and their molecular weights and distribution profiles were measured using high-performance size-exclusion chromatography with multi-angle la…
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Isolation, chemical characterization, and immunomodulatory activity of naturally acetylated hemicelluloses from bamboo shavings Open
Bamboo shavings, the outer or intermediate layer of bamboo stems, are the bulk of by-products produced in bamboo processing. In this study we investigated the isolation, chemical characterization, and immunostimulatory activity in vitro of…
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Extraction, characterization, antioxidant activity and rheological behavior of a polysaccharide produced by the extremely salt tolerant Bacillus subtilis LR-1 Open
Fermented meat possesses plenty of microbial resources. In this study, a novel exopolysaccharide (EPS) secreted by the extreme salt-tolerant Bacillus subtilis LR-1 was researched. The optimal extraction conditions of EPS in fermentation br…
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Purification, Structural Characterization, and Biological Activity of Polysaccharides from <i>Lentinus velutinus</i> Open
A polysaccharide (LVP) was purified from fruiting body of Lentinus velutinus by ethanol precipitation fractionation and DEAE and Sephadex G-100 column chromatography. The yield of purified polysaccharide was 0.025%. Molecular characteristi…
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Extraction, Purification, and Structural Characterization of Polysaccharides from Sanghuangporus vaninii with Anti-Inflammatory Activity Open
In order to obtain homogeneous Sanghuangporus vaninii polysaccharides with antioxidant and anti-inflammatory activities, a response surface method (RSM) was used to compare the polysaccharide extraction rate of hot water extraction and ult…
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Influence of Partial Acid Hydrolysis on Size, Dispersity, Monosaccharide Composition, and Conformation of Linearly-Branched Water-Soluble Polysaccharides Open
The effect of partial acid hydrolysis on the physical and chemical properties of galactomannan, arabinoxylan, and xyloglucan was investigated. Polysaccharides were treated at 50 °C with hydrochloric acid for 3–48 h. Portions of isopropanol…
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Extraction, Structural Characterization, and Anti-Hepatocellular Carcinoma Activity of Polysaccharides From Panax ginseng Meyer Open
Polysaccharides are the main active ingredients of ginseng. To extract the most effective polysaccharides against hepatocellular carcinoma (HCC), we isolated and characterized the polysaccharides from the mountain cultivated ginseng (MCG) …
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Green biorefinery for sugar beet pulp valorisation: Microwave hydrothermal processing for pectooligosaccharides recovery and biobutanol production Open
The complete valorisation of sugar beet pulp (SBP) through pectooligosaccharides (POS) extraction and biobutanol production was studied. A central composite experimental design was planned to optimise the time and temperature of the hydrot…
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Characterization and Antioxidant Activity of Mannans from Saccharomyces cerevisiae with Different Molecular Weight Open
Polysaccharides were extracted from natural sources with various biological activities, which are strongly influenced by their chemical structure and molecular weight. In this research, mannans polysaccharides were obtained from Saccharomy…