Binding protein
View article
PRODIGY: a web server for predicting the binding affinity of protein–protein complexes Open
Summary: Gaining insights into the structural determinants of protein–protein interactions holds the key for a deeper understanding of biological functions, diseases and development of therapeutics. An important aspect of this is the abili…
View article
Single-molecule imaging reveals dynamic biphasic partition of RNA-binding proteins in stress granules Open
Stress granules (SGs) are cytosolic, nonmembranous RNA–protein complexes. In vitro experiments suggested that they are formed by liquid–liquid phase separation; however, their properties in mammalian cells remain unclear. We analyzed the d…
View article
Cardiac myosin binding protein-C phosphorylation regulates the super-relaxed state of myosin Open
Significance Cardiac myosin binding protein-C (cMyBP-C) is a sarcomeric protein closely linked to cardiac contractility. Importantly, although the phosphorylation status of cMyBP-C regulates the force and rate of cardiac contraction, the e…
View article
Poly(A) tail length regulates PABPC1 expression to tune translation in the heart Open
The rate of protein synthesis in the adult heart is one of the lowest in mammalian tissues, but it increases substantially in response to stress and hypertrophic stimuli through largely obscure mechanisms. Here, we demonstrate that regulat…
View article
Protein A and Protein G Purification of Antibodies Open
Protein A and Protein G are immunoglobulin-binding proteins expressed in Staphylococcus aureus and Streptococcus sp., respectively, that have been adapted for use in purifying large amounts of IgG. They are available covalently attached to…
View article
The Glycolytic Enzyme Triosephosphate Isomerase of Trichomonas vaginalis Is a Surface-Associated Protein Induced by Glucose That Functions as a Laminin- and Fibronectin-Binding Protein Open
Triosephosphate isomerase of Trichomonas vaginalis ( Tv TIM) is a 27-kDa cytoplasmic protein encoded by two genes, tvtim1 and tvtim2 , that participates in glucose metabolism. Tv TIM is also localized to the parasite surface. Thus, the goa…
View article
Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems Open
Recombinant proteins are commonly expressed in eukaryotic expression systems to ensure the formation of disulfide bridges and proper glycosylation. Although many proteins can be expressed easily, some proteins, sub-domains, and mutant prot…
View article
Higher binding affinity of furin for SARS-CoV-2 spike (S) protein D614G mutant could be associated with higher SARS-CoV-2 infectivity Open
The D614G substitution in the G clade induced flexibility of the S protein, resulting in increased furin binding, which may enhance S protein cleavage and infiltration of host cells. Therefore, the SARS-CoV-2 D614G substitution may result …
View article
The iron chaperone and nucleic acid–binding activities of poly(rC)-binding protein 1 are separable and independently essential Open
Significance Poly(rC)-binding protein 1 (PCBP1) regulates multiple cellular processes; many studies have focused on its role in tumor formation and metastasis through its capacity to bind nucleic acids. PCBP1 binds to DNA and RNA and media…
View article
The emerging role of fatty acid binding protein 5 (FABP5) in cancers Open
Fatty acid binding protein 5 (FABP5, or epidermal FABP) is an intracellular chaperone of fatty acid molecules that regulates lipid metabolism and cell growth. In patient-derived tumours, FABP5 expression is increased up to tenfold, often c…
View article
CRF binding protein facilitates the presence of CRF type 2α receptor on the cell surface Open
Significance Corticotropin releasing factor binding protein (CRF-BP) belongs to the CRF family that is fundamental in the stress response and in the interaction between stress and addiction. The mechanisms by which CRF-BP regulates the CRF…
View article
Revisiting the Rate-Limiting Step of the ANS–Protein Binding at the Protein Surface and Inside the Hydrophobic Cavity Open
8-Anilino-1-naphthalenesulfonic acid (ANS) is used as a hydrophobic fluorescence probe due to its high intensity in hydrophobic environments, and also as a microenvironment probe because of its unique ability to exhibit peak shift and inte…
View article
Ligand Binding Induces Conformational Changes in Human Cellular Retinol-binding Protein 1 (CRBP1) Revealed by Atomic Resolution Crystal Structures Open
Important in regulating the uptake, storage, and metabolism of retinoids, cellular retinol-binding protein 1 (CRBP1) is essential for trafficking vitamin A through the cytoplasm. However, the molecular details of ligand uptake and targeted…
View article
Catechin and Curcumin interact with corona (2019-nCoV/SARS-CoV2) viral S protein and ACE2 of human cell membrane: insights from Computational study and implication for intervention Open
The recent outbreak of the coronavirus (2019n-CoV) is an unprecedented threat for human health throughout the globe. In this regards development of a suitable intervention is the need of the hour. The viral spike protein (S-Protein) and th…
View article
Apolipoprotein A-1 Binding Protein Inhibits Inflammatory Signaling Pathways by Binding to Apolipoprotein A-1 in THP-1 Macrophages Open
These results suggest that AIBP inhibits inflammatory signaling pathways through binding to apoA-1 and stabilizing ABCA1, and subsequent alteration of lipid rafts and TLR4 in the cell membrane.
View article
Glucose-regulated protein 78 substrate-binding domain alters its conformation upon EGCG inhibitor binding to nucleotide-binding domain: Molecular dynamics studies Open
Glucose-regulated protein 78 (GRP78), is overexpressed in glioblastoma, other tumors and during viral and bacterial infections, and so, it is postulated to be a key drug target. EGCG, an ATP-competitive natural inhibitor, inhibits GRP78 ef…
View article
Identification of VPS13C as a Galectin-12-Binding Protein That Regulates Galectin-12 Protein Stability and Adipogenesis Open
Galectin-12, a member of the galectin family of β-galactoside-binding animal lectins, is preferentially expressed in adipocytes and required for adipocyte differentiation in vitro. This protein was recently found to regulate lipolysis, who…
View article
The RNA-Binding Protein PCBP1 Functions as a Tumor Suppressor in Prostate Cancer by Inhibiting Mitogen Activated Protein Kinase 1 Open
Background/Aims: Poly r(C) binding protein (PCBP) 1 or heterogeneous ribonucleoprotein (hnRNP) E1 is a RNA binding protein functional in multiple biological processes. In prostate cancer (PCa), PCBP1 loss was shown to be involved with incr…
View article
Identification of Apolipoprotein A-I as a Retinoic Acid-binding Protein in the Eye Open
All-trans-retinoic acid may be an important molecular signal in the postnatal control of eye size. The goal of this study was to identify retinoic acid-binding proteins secreted by the choroid and sclera during visually guided ocular growt…
View article
Retinol-binding protein 2 (RBP2): biology and pathobiology Open
Retinol-binding protein 2 (RBP2; originally cellular retinol-binding protein, type II (CRBPII)) is a 16 kDa cytosolic protein that in the adult is localized predominantly to absorptive cells of the proximal small intestine. It is well esta…
View article
The calcium‐binding protein ALG‐2 promotes endoplasmic reticulum exit site localization and polymerization of Trk‐fused gene (TFG) protein Open
Apoptosis‐linked gene 2 ( ALG ‐2), which is a gene product of PDCD 6 , is a 22‐ kD a Ca 2+ ‐binding protein. Accumulating evidence points to a role for ALG ‐2 as a Ca 2+ ‐responsive adaptor protein. On binding to Ca 2+ , ALG ‐2 undergoes a…
View article
Removal of Protein-Bound Uremic Toxins Using Binding Competitors in Hemodialysis: A Narrative Review Open
Removal of protein-bound uremic toxins (PBUTs) during conventional dialysis is insufficient. PBUTs are associated with comorbidities and mortality in dialysis patients. Albumin is the primary carrier for PBUTs and only a small free fractio…
View article
Endothelial senescence is induced by phosphorylation and nuclear export of telomeric repeat binding factor 2–interacting protein Open
The interplay among signaling events for endothelial cell (EC) senescence, apoptosis, and activation and how these pathological conditions promote atherosclerosis in the area exposed to disturbed flow (d-flow) in concert remain unclear. Th…
View article
Opposing roles of the two isoforms of ErbB3 binding protein 1 in human cancer cells Open
The different functions of the two isoforms of ErbB3 binding protein 1 (Ebp1), p48 and p42, have recently become the focus of interest as they reveal contradictory roles in cell growth promoting ability. The conformational change that crys…
View article
ONE-HELIX PROTEIN1 and 2 Form Heterodimers to Bind Chlorophyll in Photosystem II Biogenesis Open
Members of the light-harvesting complex protein family participate in multiple processes connected with light sensing, light absorption, and pigment binding within the thylakoid membrane. Amino acid residues of the light-harvesting chlorop…
View article
RNA-binding Protein PCBP2 Regulates p73 Expression and p73-dependent Antioxidant Defense Open
TAp73, a member of the p53 family tumor suppressors, plays a critical rule in tumor suppression and neuronal development. However, how p73 activity is controlled at the posttranscriptional level is not well understood. Here, we showed that…
View article
Retinol-binding protein 2 (RBP2) binds monoacylglycerols and modulates gut endocrine signaling and body weight Open
Retinol-binding protein 2 (RBP2) binds 2-monoacylglycerols affecting their intestinal levels and enteroendocrine responses.
View article
Identification of the Light-Harvesting Chlorophyll a/b Binding Protein Gene Family in Peach (Prunus persica L.) and Their Expression under Drought Stress Open
In higher plants, light-harvesting chlorophyll a/b binding (Lhc) proteins play a vital role in photosynthetic processes and are widely involved in the regulation of plant growth, development, and response to abiotic stress. However, the Lh…
View article
Structural and functional analysis of parameters governing tankyrase-1 interaction with telomeric repeat-binding factor 1 and GDP-mannose 4,6-dehydratase Open
Human tankyrase-1 (TNKS) is a member of the poly(ADP-ribose) polymerase (PARP) superfamily of proteins that posttranslationally modify themselves and target proteins with ADP-ribose (termed PARylation). The TNKS ankyrin repeat domain media…
View article
HIV-1 NC-induced stress granule assembly and translation arrest are inhibited by the dsRNA binding protein Staufen1 Open
The nucleocapsid (NC) is an N-terminal protein derived from the HIV-1 Gag precursor polyprotein, pr55 Gag . NC possesses key functions at several pivotal stages of viral replication. For example, an interaction between NC and the host doub…