Flow cytometry ≈ Flow cytometry
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Molecular and pharmacological modulators of the tumor immune contexture revealed by deconvolution of RNA-seq data Open
We introduce quanTIseq, a method to quantify the fractions of ten immune cell types from bulk RNA-sequencing data. quanTIseq was extensively validated in blood and tumor samples using simulated, flow cytometry, and immunohistochemistry dat…
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Third-generation <i>in situ</i> hybridization chain reaction: multiplexed, quantitative, sensitive, versatile, robust Open
In situ hybridization based on the mechanism of the hybridization chain reaction (HCR) has addressed multi-decade challenges that impeded imaging of mRNA expression in diverse organisms, offering a unique combination of multiplexing, quant…
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Simultaneous enumeration of cancer and immune cell types from bulk tumor gene expression data Open
Immune cells infiltrating tumors can have important impact on tumor progression and response to therapy. We present an efficient algorithm to simultaneously estimate the fraction of cancer and immune cell types from bulk tumor gene express…
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Characteristics of Peripheral Lymphocyte Subset Alteration in COVID-19 Pneumonia Open
Background In December 2019, novel coronavirus (SARS-CoV-2) pneumonia (COVID-19) was reported in Wuhan and has since rapidly spread throughout China. We aimed to clarify the characteristics and clinical significance of peripheral lymphocyt…
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Novel Markers to Delineate Murine M1 and M2 Macrophages Open
Classically (M1) and alternatively activated (M2) macrophages exhibit distinct phenotypes and functions. It has been difficult to dissect macrophage phenotypes in vivo, where a spectrum of macrophage phenotypes exists, and also in vitro, w…
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition) Open
These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors…
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Immunophenotyping of COVID-19 and influenza highlights the role of type I interferons in development of severe COVID-19 Open
Single-cell RNA sequencing of blood immune cells reveals type I interferon–associated hyper-inflammation in severe COVID-19.
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Flow cytometry: basic principles and applications Open
Flow cytometry is a sophisticated instrument measuring multiple physical characteristics of a single cell such as size and granularity simultaneously as the cell flows in suspension through a measuring device. Its working depends on the li…
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Gene expression markers of Tumor Infiltrating Leukocytes Open
Due to their concise gene set, computational simplicity and utility in tumor samples, these cell type gene signatures may be useful in future discovery research and clinical trials to understand how tumors and therapeutic intervention shap…
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Single-Cell Analysis Reveals Fibroblast Clusters Linked to Immunotherapy Resistance in Cancer Open
A subset of cancer-associated fibroblasts (FAP+/CAF-S1) mediates immunosuppression in breast cancers, but its heterogeneity and its impact on immunotherapy response remain unknown. Here, we identify 8 CAF-S1 clusters by analyzing more than…
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Flow Cytometry: An Overview Open
Flow cytometry is a technology that provides rapid multi‐parametric analysis of single cells in solution. Flow cytometers utilize lasers as light sources to produce both scattered and fluorescent light signals that are read by detectors su…
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Carcinoma-associated fibroblasts promote the stemness and chemoresistance of colorectal cancer by transferring exosomal lncRNA H19 Open
Long non-coding RNAs (lncRNAs) are involved in the pathology of various tumors, including colorectal cancer (CRC). The crosstalk between carcinoma- associated fibroblasts (CAFs) and cancer cells in the tumor microenvironment promotes tumor…
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Exosomes derived from miR-122-modified adipose tissue-derived MSCs increase chemosensitivity of hepatocellular carcinoma Open
The findings suggest that the export of miR-122 via AMSC exosomes represents a novel strategy to enhance HCC chemosensitivity.
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Identification and isolation of a dermal lineage with intrinsic fibrogenic potential Open
Fibroblasts in fibrosis Excess fibrous connective tissue, similar to scarring, forms during the repair of injuries. Fibroblasts are known to be involved, but their role is poorly characterized. Rinkevich et al. identify two lineages of der…
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Redefining Tumor-Associated Macrophage Subpopulations and Functions in the Tumor Microenvironment Open
The immunosuppressive status of the tumor microenvironment (TME) remains poorly defined due to a lack of understanding regarding the function of tumor-associated macrophages (TAMs), which are abundant in the TME. TAMs are crucial drivers o…
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Guidelines for cell viability assays Open
Recently, the interest in the application of cell viability assays has been increasing in various fields. Cell viability assays may be broadly classified as (a) dye exclusion assays, (b) colorimetric assays, (c) fluorometric assays, (d) lu…
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Quality and efficiency assessment of six extracellular vesicle isolation methods by nano‐flow cytometry Open
Extracellular vesicles (EVs) have sparked tremendous interest owing to their prominent potential in diagnostics and therapeutics. Isolation of EVs from complex biological fluids with high purity is essential to the accurate analysis of EV …
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PD-L1 expression and prognostic impact in glioblastoma Open
The incidence of PD-L1 expression in GBM patients is frequent but is confined to a minority subpopulation, similar to other malignancies that have been profiled for PD-L1 expression. Higher expression of PD-L1 is correlated with worse outc…
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Clinical Significance of PD-L1+ Exosomes in Plasma of Head and Neck Cancer Patients Open
Purpose: The microenvironment of head and neck squamous cell carcinomas (HNSCC) is highly immunosuppressive. HNSCCs expressing elevated levels of PD-L1 have especially poor outcome. Exosomes that carry PD-L1 and suppress T-cell functions h…
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Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method Open
Single-cell analysis reveals aspects of cellular physiology not evident from population-based studies, particularly in the case of highly multiplexed methods such as mass cytometry (CyTOF) able to correlate the levels of multiple signallin…
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Microvesicle- and exosome-mediated drug delivery enhances the cytotoxicity of Paclitaxel in autologous prostate cancer cells Open
Cancer cell-derived EVs can be used as effective carriers of Paclitaxel to their parental cells, bringing the drug into the cells through an endocytic pathway and increasing its cytotoxicity. However, due to the increased cell viability, t…
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Affinity-Tuned ErbB2 or EGFR Chimeric Antigen Receptor T Cells Exhibit an Increased Therapeutic Index against Tumors in Mice Open
Target-mediated toxicity is a major limitation in the development of chimeric antigen T-cell receptors (CAR) for adoptive cell therapy of solid tumors. In this study, we developed a strategy to adjust the affinities of the scFv component o…
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Guidelines for the use of flow cytometry and cell sorting in immunological studies <sup>*</sup> Open
The editorial team of the European Journal of
\nImmunology feels it is worthwhile to offer our community guidelines
\nfor the correct use of cytometric techniques in the field of immunology.
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High‐speed centrifugation induces aggregation of extracellular vesicles Open
Plasma and other body fluids contain cell‐derived extracellular vesicles (EVs), which participate in physiopathological processes and have potential biomedical applications. In order to isolate, concentrate and purify EVs, high‐speed centr…
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Atlas of the Immune Cell Repertoire in Mouse Atherosclerosis Defined by Single-Cell RNA-Sequencing and Mass Cytometry Open
Rationale: Atherosclerosis is a chronic inflammatory disease that is driven by the interplay of pro- and anti-inflammatory leukocytes in the aorta. Yet, the phenotypic and transcriptional diversity of aortic leukocytes is poorly understood…
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Tumor immune profiling predicts response to anti–PD-1 therapy in human melanoma Open
This work was funded by a generous gift provided by Inga-Lill and David Amoroso as well as a generous gift provided by Stephen Juelsgaard and Lori Cook.
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Tumour-activated neutrophils in gastric cancer foster immune suppression and disease progression through GM-CSF-PD-L1 pathway Open
Objective Neutrophils are prominent components of solid tumours and exhibit distinct phenotypes in different tumour microenvironments. However, the nature, regulation, function and clinical relevance of neutrophils in human gastric cancer …
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Low-density lipoprotein mimics blood plasma-derived exosomes and microvesicles during isolation and detection Open
Circulating extracellular vesicles have emerged as potential new biomarkers in a wide variety of diseases. Despite the increasing interest, their isolation and purification from body fluids remains challenging. Here we studied human pre-pr…
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Biology and relevance of human acute myeloid leukemia stem cells Open
Evidence of human acute myeloid leukemia stem cells (AML LSCs) was first reported nearly 2 decades ago through the identification of rare subpopulations of engrafting cells in xenotransplantation assays. These AML LSCs were shown to reside…
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Natural killer cell immunotypes related to COVID-19 disease severity Open
The NK cell activation landscape in acute SARS-CoV-2 infection is associated with COVID-19 disease severity.