Microtubule-associated protein

Tau Protein The Brain Stabilizer, and Treatment by N-K Model.pdf Open

Malik Muhammad Usman · 2025

بِسْمِ اللَّهِ الرَّحْمَٰنِ الرَّحِيمِ ZENODO DEPOSITION DESCRIPTION Title: Tau Protein: The Brain Stabilizer and Complete Alzheimer's Treatment Protocol by N-K Sacred Model Authors: Muhammad Usman Malik Description: This Zenodo deposition presents the re…

Tau Protein The Brain Stabilizer, and Treatment by N-K Model.pdf Open

Malik Muhammad Usman · 2025

بِسْمِ اللَّهِ الرَّحْمَٰنِ الرَّحِيمِ ZENODO DEPOSITION DESCRIPTION Title: Tau Protein: The Brain Stabilizer and Complete Alzheimer's Treatment Protocol by N-K Sacred Model Authors: Muhammad Usman Malik Description: This Zenodo deposition presents the re…

Tau–Mitochondria Interactions in Neurodegeneration: Mechanisms and Therapeutic Potential Open

Yaisa Castillo Casaña, Clorinda Arias, Roberto Coria, Yaisa Castillo Casaña, Clorinda Arias , et al. · 2025

Tau is a microtubule-associated protein encoded by the MAPT gene and is mainly expressed in neurons. Alternative splicing generates preferentially six isoforms differing in N-terminal inserts (0, 1, or 2N) and microtubule-binding repeats (…

Injury-induced tau pathology promotes aggressive behavior in Drosophila without neurodegeneration. Open

Roilea Maxson, Christine J. Smoyer, Megan F Hampton, Yu-Sheng Shen, Kailea Wiese , et al. · 2025

The microtubule-associated protein tau is implicated in neurodegenerative diseases, but its physiological roles remain poorly understood. Here, we find that pan-neuronal expression of human tau (HsTau) in Drosophila coupled with injury tri…

Structure of the microtubule associated protein PRC1 (Protein Regulator of Cytokinesis 1) Open

NIH 3D · 2025

Reviewer #1 (Public review): MCAK recognizes the nucleotide-dependent feature at growing microtubule ends Open

2025

MCAK recognizes the nucleotide-dependent feature at growing microtubule ends Open

Wei Chen, Yin-Long Song, Jian Feng He, Xin Liang · 2025

The growing plus-end is a key regulatory site for microtubule dynamics. MCAK (mitotic centromere-associated kinesin), a microtubule depolymerizing kinesin, is an end-binding regulator of catastrophe frequency. It is intriguing how MCAK spe…

Reviewer #4 (Public review): MCAK recognizes the nucleotide-dependent feature at growing microtubule ends Open

2025

Microtubule dependent changes in cell polarity are required for mammary epithelial cell migration and branching morphogenesis Open

Andrew K. Fraser, Joshua Carreras, Isabel A. Ryan, Jennifer Zoll, Andrew J. Ewald · 2025

Branching morphogenesis requires dramatic changes in cell polarity, proliferation, migration, and actin dynamics to elaborate tubular networks. However, little is known about how microtubules support these cell behaviors in 3D tissues. Usi…

Structure and mechanism of microtubule stabilization and motor regulation by MAP9 Open

Burak Çetin, Aryan Taheri, Mert Golcuk, Brigette Y. Monroy, Jonathan Fernandes , et al. · 2025

Microtubule-associated proteins (MAPs) regulate the organization of microtubules and control intracellular transport, but their individual contributions to microtubule dynamics and motor regulation remain poorly understood. Here, we identi…

NuMA and Ninein: Dynein Cargo-Adaptors Without a Classical Cargo Open

Keying Guo, Andreas Merdes, Keying Guo, Andreas Merdes · 2025

Dynein is a minus-end-directed microtubule motor that transports a variety of cargoes. Cargo specificity is mediated by a class of adaptor proteins that bind to the interface between dynein and dynactin, along the length of the Arp1 filame…

Brain-derived synaptic vesicles have an intrinsic ability to sequester tubulin Open

Tiago Mimoso, Aleksandr Korobeinikov, Alexander Stein, Dragomir Milovanovic, Silvio O. Rizzoli , et al. · 2025

Background The presence and function of microtubules within the synaptic bouton has long been under investigation. In recent years, evidence has accumulated that connects the synaptic vesicle cluster to the local dynamics of microtubule en…

Prefoldin 5 is a microtubule-associated protein that suppresses Tau-aggregation and neurotoxicity Open

Anjali Bisht, Srikanth Pippadpally, Snehasis Majumder, Athulya T Gopi, Abhijit Das , et al. · 2025

Tauopathies represent a major class of neurodegenerative disorders associated with intracellular aggregates of the microtubule-associated protein Tau. To identify molecular modulators of Tau toxicity, we used a genetic screen to identify p…

Chlamydomonas protein kinase MAK phosphorylates FAP256/CEP104 and regulates axonemal microtubule assembly Open

Yi Zhang, Xinjia Yang, Jun-Min Pan · 2025

The formation of cilia involves the assembly of axonemal microtubules, the structural core of the organelle, from tubulins that are delivered by intraflagellar transport and diffusion. Whether and how this process is controlled are not cle…

An atlas of microtubule lattice parameters regulated through ligand binding to the microtubule stabilizing sites Open

D. Lucena Agell, O. Fernández, R. París-Ogáyar, J. Estevez Gallego, D. Ondruskova , et al. · 2025

Microtubules (MTs) are essential cytoskeletal polymers with dual functions: they generate mechanical forces necessary for processes such as mitosis and angiogenesis, and serve as static tracks for intracellular transport by motor proteins.…

The Dishevelled C-terminus interacts with the centrosomal protein Kizuna to regulate microtubule organization during ciliogenesis Open

Maya Lines, Kenan Murray, Anthea Luo, Taewoo Yang, Yoo-Seok Hwang , et al. · 2025

Microtubules (MTs) serve as dynamic scaffolds that support diverse cellular processes, including cell division, intracellular transport, and epithelial morphogenesis. In ciliated cells, MTs not only form the axonemal backbone of cilia but …

Arabidopsis AUGMIN8 Contains Two Independent Microtubule Association Domains Open

Naveen K. Chana, Timothy Cioffi, Sidney L. Shaw, Naveen K. Chana, Timothy Cioffi , et al. · 2025

Plant cells create a plasma membrane‐associated network of microtubules that are nucleated by γ‐tubulin ring complexes primarily through microtubule‐dependent microtubule nucleation (MDMN). This dynamic array organizes into specific patter…

Kinesins in Caenorhabditis elegans neuronal morphogenesis Open

Shinsuke Niwa, Kyoko Chiba · 2025

Neuronal morphogenesis is regulated by intracellular transport and cytoskeletal dynamics. Kinesin superfamily proteins (KIFs), or kinesins, function as molecular motors for intracellular transport and as regulators of the microtubule cytos…

Fluorescent Colchicine for Investigating Mitochondrial–Microtubule Interactions Open

Yogesh Dubey, Sriram Kanvah, Yogesh Dubey, Sriram Kanvah · 2025

Understanding organelle interactions is crucial for unravelling the cellular mechanisms underlying apoptosis, particularly in cancer therapy. Microtubules provide structural and functional support to organelles, and their disruption can ca…

ACLY regulates autolysosome acidification through tubulin acetylation‐mediated assembly of V‐ATPase subunits in Alzheimer's disease model mice Open

Anlan Lin, Xiaoman Dai, Jianmin Chen, Tianqing Han, Qiang Du , et al. · 2025

INTRODUCTION Faulty autolysosome acidification leads to dystrophic neurites—an early event propelling Alzheimer's disease (AD) progression—yet the underlying mechanism remains elusive. METHODS To elucidate the physiological functions of ne…

Structure, interactions, and dynamic self-assembly of tubulin with different tau isoforms Open

Ben Nun, Itai, Cohen Ariel, Fellig, Amos, Laughlin, Patrick, Meidan, Ofir , et al. · 2025

Tubulin nucleation and microtubule (MT) assembly are frequent events in cells. The mechanisms directing these processes are poorly understood, owing to the size of tubulin and its highly dynamic character. The MT-associated tau proteins, i…

CDKL5 regulates the initiation of retrograde axonal transport through CLIP170 –dynactin complex formation Open

Serena Baldin, Claudia Carmone, Giorgia Valetti, Roberta De Rosa, Isabella Barbiero · 2025

Cyclin‐dependent kinase‐like 5 (CDKL5) is a serine–threonine kinase implicated in regulating microtubule (MT) dynamics. Mutations in CDKL5 are associated with a rare neurodevelopmental disease called CDKL5 deficiency disorder (CDD), which …

MATCAP1 preferentially binds an expanded tubulin conformation to generate detyrosinated and ΔC2 α-tubulin Open

Yue Yang, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey · 2025

Microtubules are cytoskeletal filaments with critical roles in cell division, cell motility, intracellular trafficking, and cilium function. In cells, subsets of microtubules are selectively marked by posttranslational modifications (PTMs)…

MCAK Recognizes the Nucleotide-Dependent Feature at Growing Microtubule Ends Open

Wei Chen, Yin-Long Song, Jian Feng He, Xin Liang · 2025

The growing plus-end is a key regulatory site for microtubule dynamics. MCAK (mitotic centromere-associated kinesin), a microtubule depolymerizing kinesin, is an end-binding regulator of catastrophe frequency. It is intriguing how MCAK spe…

Specific Combinations of Physiological Tau Phosphorylation Regulate Tau-Microtubule Interactions in Developing Neurons Open

Nima Naseri, Tian Xu, Sapanna Chantarawong, Steven M. Patrie, Neil L. Kelleher , et al. · 2025

Tau phosphorylation is a defining feature of Alzheimer's disease, yet it also plays an essential physiological role in stabilizing microtubules (MTs) during normal neuronal development. While individual phosphorylation sites have been well…

MAP2 phosphorylation: mechanisms, functional consequences, and emerging insights Open

Jiali Lyu, Andrew G. DeMarco, Robert A. Sweet, Melanie Grubisha · 2025

Microtubule-associated protein 2 (MAP2) is a key regulator of cytoskeletal dynamics and neuronal function. It stabilizes microtubules, shapes dendrites, influences synaptic plasticity, and regulates transportation and protein synthesis thr…

Reviewer #2 (Public review): The microtubule-binding protein EML3 is required for mammalian embryonic growth and cerebral cortical development; Eml3 null mice are a model of cobblestone brain malformation Open

2025

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays Open

Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi , et al. · 2025

Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐transl…

A tubulin-binding protein that preferentially binds to GDP-tubulin and promotes GTP exchange Open

Wesley J. Yon, Taekjip Ha, Yixian Zheng, Ross T.A. Pedersen · 2025

Engine breakdown of lysosomes and related organelles and the resulting physiology Open

Nina Bakker, Marlieke L.M. Jongsma, Jacques Neefjes · 2025

Late endosomes/lysosomes (LE/Lys) and lysosome related organelles (LROs) move dynamically through cells which involves many levels of regulation. To reach their destination, they need to connect to the motor proteins dynein-dynactin, kines…