DNA synthesis
View article: Table 1_Heat stress suppresses DNA replication and mitosis in barley root apical meristems.xlsx
Table 1_Heat stress suppresses DNA replication and mitosis in barley root apical meristems.xlsx Open
Increasing temperature affects plant development, with the assumption that roots are among the tissues particularly sensitive to heat stress (HS). However, a comprehensive analysis of the impact of high temperature on the dynamics of cell …
View article: Video 1_Heat stress suppresses DNA replication and mitosis in barley root apical meristems.mp4
Video 1_Heat stress suppresses DNA replication and mitosis in barley root apical meristems.mp4 Open
Increasing temperature affects plant development, with the assumption that roots are among the tissues particularly sensitive to heat stress (HS). However, a comprehensive analysis of the impact of high temperature on the dynamics of cell …
View article: Data Sheet 1_Heat stress suppresses DNA replication and mitosis in barley root apical meristems.pdf
Data Sheet 1_Heat stress suppresses DNA replication and mitosis in barley root apical meristems.pdf Open
Increasing temperature affects plant development, with the assumption that roots are among the tissues particularly sensitive to heat stress (HS). However, a comprehensive analysis of the impact of high temperature on the dynamics of cell …
View article: Radioprotective and Radiomitigative Effects of Resveratrol in Radiation-Induced Reproductive Toxicity in Male Mice
Radioprotective and Radiomitigative Effects of Resveratrol in Radiation-Induced Reproductive Toxicity in Male Mice Open
Background: Agents with free radical-scavenging functions may act as radiation modifiers, protectors, or mitigators. Methods: We investigated whether supplementation with resveratrol (RSV) in mice, at different times after the beginning of…
View article: Engineeringand Assay of Enzymes for Expanded GeneticAlphabet Replication
Engineeringand Assay of Enzymes for Expanded GeneticAlphabet Replication Open
Use of noncanonical nucleotides for nucleic acid replication provides a critical way to understand how and why natural nucleotides were chosen by evolution and provides potential applications in biotechnology, diagnostics, therapeutics, an…
View article: Characterisation of ATR kinase mutants in <i>Leishmania major.</i>
Characterisation of ATR kinase mutants in <i>Leishmania major.</i> Open
(A) Growth curve of the indicated cells cultivated in HOMEM medium; cells were seeded at ~2x105 cells.ml-1 at day 0; growth was evaluated for 6 days and cell density assessed every 24 hours; CC1 and Cas9T7 cells were …
View article: Text file of script for sequence analysis.
Text file of script for sequence analysis. Open
All cells possess mechanisms to maintain and replicate their genomes, whose integrity and transmission are constantly challenged by DNA damage and replication impediments. In eukaryotes, the protein kinase Ataxia-Telangiectasia and Rad3-r…
View article: Replication stress leads to low DNA synthesis intensity in mycATRΔC-/- cells.
Replication stress leads to low DNA synthesis intensity in mycATRΔC-/- cells. Open
EdU intensity (A.U.) from positive EdU mycATR or mycATRΔC-/- cells in each condition showed in Fig 3E. An area was drawn around each EdU positive cell’s nucleus based on the DAPI stain and the EdU signal intensity was measured using ImageJ…
View article: Supplementary Materials and Methods from Sequential Targeting of Retinoblastoma and DNA Synthesis Pathways Is a Therapeutic Strategy for Sarcomas That Can Be Monitored in Real Time
Supplementary Materials and Methods from Sequential Targeting of Retinoblastoma and DNA Synthesis Pathways Is a Therapeutic Strategy for Sarcomas That Can Be Monitored in Real Time Open
This file contains the description of supplementary materials and methods that is referred to in the manuscript
View article: Table showing all primers used in this study.
Table showing all primers used in this study. Open
All cells possess mechanisms to maintain and replicate their genomes, whose integrity and transmission are constantly challenged by DNA damage and replication impediments. In eukaryotes, the protein kinase Ataxia-Telangiectasia and Rad3-r…
View article: Supplementary Tables from Sequential Targeting of Retinoblastoma and DNA Synthesis Pathways Is a Therapeutic Strategy for Sarcomas That Can Be Monitored in Real Time
Supplementary Tables from Sequential Targeting of Retinoblastoma and DNA Synthesis Pathways Is a Therapeutic Strategy for Sarcomas That Can Be Monitored in Real Time Open
This file contains 6 supplementary tables referred to in the text of the manuscript
View article: Identification of Fibrillarin and Cajal Bodies Under DNA Replication Stress Conditions in Root Meristem Cells of Allium cepa
Identification of Fibrillarin and Cajal Bodies Under DNA Replication Stress Conditions in Root Meristem Cells of Allium cepa Open
The correct course of DNA replication is crucial to maintaining the integrity of the genome. Any abnormality in this process inevitably leads to replication stress (RS). Hydroxyurea (HU) is a replication stressor widely used to inhibit DNA…
View article: Correction to “Transcriptomic and DNA Methylomic Defects in In Vitro Matured Lamb Oocytes and Potential Improvement of Their Development by Supplementing Phorbol Myristate Acetate During In Vitro Maturation”
Correction to “Transcriptomic and DNA Methylomic Defects in In Vitro Matured Lamb Oocytes and Potential Improvement of Their Development by Supplementing Phorbol Myristate Acetate During In Vitro Maturation” Open
View article: Genome integrity relies on rapid recycling of DNA Pol III in bacteria
Genome integrity relies on rapid recycling of DNA Pol III in bacteria Open
DNA replication requires precise coordination between DNA unwinding and DNA synthesis. In all domains of life, protein–protein interactions at the replisome maintain proximity between the enzymes that catalyze these two activities. Surpris…
View article: PAF15 stabilizes PCNA on DNA and slows down its sliding dynamics
PAF15 stabilizes PCNA on DNA and slows down its sliding dynamics Open
Summary The PCNA-associated factor 15 (PAF15) protein is essential for human genome stability by regulating DNA replication and repair through its interaction with proliferating cell nuclear antigen (PCNA). Despite its significance, the me…
View article: LEM-3/ANKLE1 nuclease prevents the formation of syncytium between postmitotic sister cells and safeguards neuronal differentiation
LEM-3/ANKLE1 nuclease prevents the formation of syncytium between postmitotic sister cells and safeguards neuronal differentiation Open
In animal development, cells exit the cell cycle after a final division to differentiate into specialized types. However, the impact of failed cytokinesis on the fate specification of postmitotic cells, especially neurons, remain poorly un…
View article: Insulin production is sustained during DNA damage-mediated senescence in adult human beta cells
Insulin production is sustained during DNA damage-mediated senescence in adult human beta cells Open
Our findings suggest that the expression of proinsulin-processing enzymes and the production of insulin are sustained in both chemically induced DNA damage-related senescence and in endogenous senescent adult human beta cells. Collectively…
View article: Identification of determinants of high-fidelity DNA synthesis in <i>Mycobacterium smegmatis</i> DnaE1 through <i>in silico</i> and <i>in vivo</i> approaches
Identification of determinants of high-fidelity DNA synthesis in <i>Mycobacterium smegmatis</i> DnaE1 through <i>in silico</i> and <i>in vivo</i> approaches Open
Drug resistance in Mycobacterium tuberculosis presents a major challenge in tuberculosis treatment, highlighting the need to understand the underlying mechanisms. DNA replication plays an important role in the acquisition of drug resistanc…
View article: Adaptive nuclear GTP synthesis promotes glioblastoma treatment resistance and is a targetable vulnerability in patients
Adaptive nuclear GTP synthesis promotes glioblastoma treatment resistance and is a targetable vulnerability in patients Open
Glioblastoma (GBM), the most lethal of all brain cancers, resists therapy by rewiring metabolism and relying on GTP signaling to promote DNA repair and radiation therapy (RT) resistance. How GBM modulates GTP levels for this signaling in r…
View article: Developmental control of DNA damage responses in α- and β-cells shapes the selective beta-cell susceptibility in diabetes
Developmental control of DNA damage responses in α- and β-cells shapes the selective beta-cell susceptibility in diabetes Open
Accumulation of DNA damage drives β-cell dysfunction, senescence, and death in Type 1 and Type 2 diabetes. While α-cell dysfunction also contributes to disease pathology, they remain remarkably resistant to senescence and cell-death. The m…
View article: Quinoline-Based DNA Methyltransferase Inhibitors Featuring Basic Side Chains: Design, Synthesis, and Insight in Biochemical and Anticancer Cell Properties
Quinoline-Based DNA Methyltransferase Inhibitors Featuring Basic Side Chains: Design, Synthesis, and Insight in Biochemical and Anticancer Cell Properties Open
The quinoline DNMT inhibitors 4-21, incorporating basic chains, were designed, synthesized, and evaluated for their ability to inhibit DNMT1 and DNMT3A/3L by directly measuring DNA methylation. Pharmacomodulation yield…
View article: To Investigate the Functional Interplay Between TDR1 and the DNA Repair Pathway in Tardigrades Following Exposure to Ionizing Radiation
To Investigate the Functional Interplay Between TDR1 and the DNA Repair Pathway in Tardigrades Following Exposure to Ionizing Radiation Open
Tardigrades are renowned for their remarkable tolerance to extreme conditions. Their resistance relies not only on some protective proteins such as DSUP, but also on highly efficient DNA repair mechanisms. A novel tardigrade-specific gene,…
View article: Visualizing the Invisible:Dual Click Imaging of Ruthenium-BasedPhotoactivated Chemotherapy Agents and Their DNA Synthesis Inhibitionin Fixed Cancer Cells
Visualizing the Invisible:Dual Click Imaging of Ruthenium-BasedPhotoactivated Chemotherapy Agents and Their DNA Synthesis Inhibitionin Fixed Cancer Cells Open
Like many drugs, ruthenium-based photoactivated chemotherapy (PACT) complexes are hard to follow in cells due to their absence of emissive properties. Here, two alkyne-functionalized Ru-based PACT compounds with the formula [Ru(HCC-tpy)(N̂N…
View article: Visualizing the Invisible:Dual Click Imaging of Ruthenium-BasedPhotoactivated Chemotherapy Agents and Their DNA Synthesis Inhibitionin Fixed Cancer Cells
Visualizing the Invisible:Dual Click Imaging of Ruthenium-BasedPhotoactivated Chemotherapy Agents and Their DNA Synthesis Inhibitionin Fixed Cancer Cells Open
Like many drugs, ruthenium-based photoactivated chemotherapy (PACT) complexes are hard to follow in cells due to their absence of emissive properties. Here, two alkyne-functionalized Ru-based PACT compounds with the formula [Ru(HCC-tpy)(N̂N…
View article: Data from: Real-time imaging of rotation during synthesis by the replisome
Data from: Real-time imaging of rotation during synthesis by the replisome Open
During chromosome replication, unwinding by the helicase and synthesis by the polymerases can lead to overwinding and supercoiling of DNA. The mechanical consequences of these events and resulting local dynamics at the replication fork are…
View article: Microwave‐Enhanced Synthesis of 2‐Styrylquinoline‐4‐Carboxamides With Promising Anti‐Lymphoma Activity
Microwave‐Enhanced Synthesis of 2‐Styrylquinoline‐4‐Carboxamides With Promising Anti‐Lymphoma Activity Open
Diffuse large B‐cell lymphoma (DLBCL) is the most common subtype of non‐Hodgkin lymphoma, characterized by significant clinical and molecular heterogeneity. Here, we report the design and synthesis of a novel series of 2‐styrylquinoline‐4‐…
View article: DDDR-33. EO-4426: A brain-penetrant dual DNA-polymerase α and ribonucleotide reductase inhibitor for high-grade gliomas and other aggressive cancers
DDDR-33. EO-4426: A brain-penetrant dual DNA-polymerase α and ribonucleotide reductase inhibitor for high-grade gliomas and other aggressive cancers Open
BACKGROUND High-grade gliomas (HGG) and other CNS malignancies are marked by replication stress, genomic instability, and resistance to standard-of-care therapies. EO-4426 (tezacitabine) is an orally bioavailable, brain-penetrant small mol…
View article: RBIO-05. Mitochondrial complex I inhibitor, IACS synergistically enhances the radio-therapeutic effect for the intracranial S180 sarcoma mouse model
RBIO-05. Mitochondrial complex I inhibitor, IACS synergistically enhances the radio-therapeutic effect for the intracranial S180 sarcoma mouse model Open
BACKGROUND Radiotherapy (RT) eliminates cancer cells by inducing DNA damage. However, its efficacy is often reduced by mechanisms such as radiation resistance, effective DNA repair, and hypoxia within tumors. Tumor cells utilize metabolic …
View article: Inhibition of kinase HASPIN as a novel anti-tumor strategy to radiosensitize and boost the in situ vaccination effect of radiation 9073
Inhibition of kinase HASPIN as a novel anti-tumor strategy to radiosensitize and boost the in situ vaccination effect of radiation 9073 Open
Description Radiation therapy (RT) is a treatment used in roughly half of all cancer patients at some point over the course of their care. RT induces DNA damage that can kill cells if not properly repaired. DNA damaged by RT may also leak …
View article: The pattern recognition receptors cGAS and IFI16 do not bind and initiate STING-dependent IFN-I signaling on dsDNA fragments containing deoxy-uracil 4473
The pattern recognition receptors cGAS and IFI16 do not bind and initiate STING-dependent IFN-I signaling on dsDNA fragments containing deoxy-uracil 4473 Open
Description DNA damage and cytoplasmic DNA fragments induce type-1 interferons (IFN-1) and enhance responses to immune checkpoint inhibitors. Our previous work showed that ATR inhibitors (ATRi), which target DNA damage response kinases, in…