Single particle analysis
View article: From Photon to Protein: Using X-ray Lasers for High-resolution Structure Determination with Single Proteins
From Photon to Protein: Using X-ray Lasers for High-resolution Structure Determination with Single Proteins Open
X-ray Free-Electron Lasers (XFELs) promise Single Particle Imaging (SPI) of biomolecules through “diffraction before destruction,” but present experiments involving single proteins are limited by weak scattering signals and significant bac…
View article: Southern Germany cryo-EM BAG: Structural analysis of protein machineries using single particle cryo-EM
Southern Germany cryo-EM BAG: Structural analysis of protein machineries using single particle cryo-EM Open
Cryogenic single-particle electron microscopy (Cryo-EM) has become the method of choice for determining the structures of large macromolecular complexes and membrane proteins – targets that have been challenging for X-ray crystallography. …
View article: InvestigatingSpatial Heterogeneity in Aerosols ChemicalComposition and Implications for Physico-Optical Properties over theNorth Eastern Region of India
InvestigatingSpatial Heterogeneity in Aerosols ChemicalComposition and Implications for Physico-Optical Properties over theNorth Eastern Region of India Open
Total suspended particulates (TSP) samples were collected using a high-volume sampler in a campaign mode observation over seven selected locations in North-East India, during post-monsoon season of 2023. Scanning electron microscopy (SEM) …
View article: Contrasting solubility and speciation of metal ions in total suspended particulate matter and fog from the coast of Namibia – Part 1
Contrasting solubility and speciation of metal ions in total suspended particulate matter and fog from the coast of Namibia – Part 1 Open
The western coast of southern Africa is a region of particular climate interest and crossroads for aerosols of different origins as well as fog occurrences. In this study, we present a comparison between the concentration of dissolved trac…
View article: Automated filtering of particle images in single particle cryoEM
Automated filtering of particle images in single particle cryoEM Open
Continued exponential growth in the number of structures resolved by single particle cryoEM, as seen in the last decade, requires ever more effective data analysis workflows. Datasets are rarely homogeneous, demanding a multistep procedure…
View article: Unraveling theFormation and Evolution of Winter Hazein Beijing: Insights from Vertical Measurements of Particle Size Distributionsand Aerosol Composition
Unraveling theFormation and Evolution of Winter Hazein Beijing: Insights from Vertical Measurements of Particle Size Distributionsand Aerosol Composition Open
Understanding the dynamics of haze formation is essential for addressing air quality and public health concerns in megacities. In this study, we present vertical measurements of size-resolved particle number concentrations and aerosol comp…
View article: PickET: An unsupervised method for localizing macromolecules in cryo-electron tomograms
PickET: An unsupervised method for localizing macromolecules in cryo-electron tomograms Open
Cryo-electron tomography (cryo-ET) datasets are rich sources of information capable of describing the localizations, structures, and interactions of macromolecules. However, most current methods for localizing particles in cryo-electron to…
View article: Source Identificationof Atmospheric Particles viaLow-Voltage Electron Microscopy Image Recognition: A Case Study ofSubmicrometer Particles
Source Identificationof Atmospheric Particles viaLow-Voltage Electron Microscopy Image Recognition: A Case Study ofSubmicrometer Particles Open
Atmospheric particulate matter from different sources exhibits distinct morphological characteristics, which can be automatically extracted through deep learning for source identification. Computer-controlled scanning electron microscopy (…
View article: Spatial MolecularHeterogeneity on BiofunctionalizedParticles Quantified by Three-Dimensional Single-Molecule DNA-PAINT
Spatial MolecularHeterogeneity on BiofunctionalizedParticles Quantified by Three-Dimensional Single-Molecule DNA-PAINT Open
Quantifying and controlling the spatial molecular heterogeneity on biofunctionalized particles is essential for understanding and improving their functionality in bioscience applications. Here, we describe an analysis framework based on si…
View article: Spatial MolecularHeterogeneity on BiofunctionalizedParticles Quantified by Three-Dimensional Single-Molecule DNA-PAINT
Spatial MolecularHeterogeneity on BiofunctionalizedParticles Quantified by Three-Dimensional Single-Molecule DNA-PAINT Open
Quantifying and controlling the spatial molecular heterogeneity on biofunctionalized particles is essential for understanding and improving their functionality in bioscience applications. Here, we describe an analysis framework based on si…
View article: Performance analysis of single particle deposition behavior on different surfaces
Performance analysis of single particle deposition behavior on different surfaces Open
Airborne dust deposition has become a serious problem in many energy and environmental devices. Determining the single-particle deposition behavior may be helpful in designing the anti-dust surface. Hence, in this study, the single particl…
View article: Comparison of PM source profiles identified by different techniques and the potential of utilizing single-particle analysis data in source apportionment
Comparison of PM source profiles identified by different techniques and the potential of utilizing single-particle analysis data in source apportionment Open
View article: Sub-3 Å resolution protein structure determination by single-particle cryo-EM at 100 keV
Sub-3 Å resolution protein structure determination by single-particle cryo-EM at 100 keV Open
Cryoelectron microscopy (cryo-EM) has transformed structural biology by providing high-resolution insights into biological macromolecules. We report sub-3 Å resolution structures using the 100 keV Tundra cryo-TEM, equipped with the Falcon …
View article: Self-supervised learning for generalizable particle picking in cryo-EM micrographs
Self-supervised learning for generalizable particle picking in cryo-EM micrographs Open
We present cryoelectron microscopy masked autoencoder (cryo-EMMAE), a self-supervised method designed to overcome the need for manually annotated cryo-EM data. cryo-EMMAE leverages the representation space of a masked autoencoder to pick p…
View article: Probing Early Particle‐Cell Membrane Interactions via Single‐Cell and Single‐Particle Interaction Analysis
Probing Early Particle‐Cell Membrane Interactions via Single‐Cell and Single‐Particle Interaction Analysis Open
Endocytosis is vital for nutrient uptake and nanomedicine applications, but the biophysics of the pre‐internalization phase remains poorly understood at single‐cell level. This study uses advanced robotic techniques to analyze pre‐internal…
View article: Three-dimensional single-particle reconstruction by atomic force microscopy allows rapid structural-based validation of recombinant SARS-CoV-2 Spike protein from a single topology image
Three-dimensional single-particle reconstruction by atomic force microscopy allows rapid structural-based validation of recombinant SARS-CoV-2 Spike protein from a single topology image Open
Atomic force microscopy (AFM) is a versatile multi-modal imaging method frequently used for structural characterisation of biological surfaces at the nanoscale. However, AFM-based three-dimensional single-particle reconstruction has hither…
View article: Single particle cryo-EM micrographs and particles of OLE RNA dimer
Single particle cryo-EM micrographs and particles of OLE RNA dimer Open
View article: A guide for Single Particle Tracking: from sample preparation and image acquisition to the analysis of individual trajectories
A guide for Single Particle Tracking: from sample preparation and image acquisition to the analysis of individual trajectories Open
Live-cell Single-Particle Tracking (SPT) is a powerful super resolution microscopy approach. It allows us to observe live recordings of individual molecules in living cells at high temporal and spatial resolution (100 Hz, 20 nm). Here, we …
View article: Fast Single-Particle Tracking of Membrane Proteins Combined with Super-Resolution Imaging of Actin Nanodomains
Fast Single-Particle Tracking of Membrane Proteins Combined with Super-Resolution Imaging of Actin Nanodomains Open
Membrane protein dynamics regulates cell functions by initiating downstream signaling cascades. The cell membrane is compartmentalized into nanodomains by actin structures, restricting lateral protein diffusion. Single-particle tracking of…
View article: Chemical Imagingof Atmospheric Biomass Burning Particlesfrom North American Wildfires
Chemical Imagingof Atmospheric Biomass Burning Particlesfrom North American Wildfires Open
The effects of biomass burning aerosols (BBA) on radiative forcing and cloud formation depend on chemical composition and the internal structures of individual particles within smoke plumes. To improve our understanding of the chemical and…
View article: PiP-plex: A Particle-in-Particle System for Multiplexed Quantification of Proteins Secreted by Single Cells
PiP-plex: A Particle-in-Particle System for Multiplexed Quantification of Proteins Secreted by Single Cells Open
Cell signaling is modulated by the secretion of various proteins, which can be used to infer a cell’s phenotype. However, these proteins cannot be readily detected in multiplex by commonly used methods at the single cell level. Here, we pr…
View article: Unveiling single-particle composition, size, shape, and mixing state of freshly emitted Icelandic dust via electron microscopy analysis
Unveiling single-particle composition, size, shape, and mixing state of freshly emitted Icelandic dust via electron microscopy analysis Open
Iceland is a major high-latitude dust source area. Airborne Icelandic dust influences the climate system via interacting with radiation, clouds, and biogeochemical systems, impacts the snow/ice albedo, and air quality. These impacts are se…
View article: Supplementary material to "Unveiling single-particle composition, size, shape, and mixing state of freshly emitted Icelandic dust via electron microscopy analysis"
Supplementary material to "Unveiling single-particle composition, size, shape, and mixing state of freshly emitted Icelandic dust via electron microscopy analysis" Open
View article: Deconvolution to restore cryo-EM maps with anisotropic resolution
Deconvolution to restore cryo-EM maps with anisotropic resolution Open
View article: Unraveling atomic complexity from frozen samples
Unraveling atomic complexity from frozen samples Open
Cryo-electron microscopy (cryo-EM) is a significant driver of recent advances in structural biology. Cryo-EM is comprised of several distinct and complementary methods, which include single particle analysis, cryo-electron tomography, and …
View article: Deconvolution to restore cryo-EM maps with anisotropic resolution
Deconvolution to restore cryo-EM maps with anisotropic resolution Open
With technological advancements in recent years, single particle cryogenic electron microscopy (cryo-EM) has become a major methodology for structural biology. Structure determination by single particle cryo-EM is premised on randomly orie…
View article: Characterizing lead-rich particles in Beijing's atmosphere following coal-to-gas conversion: Insights from single particle aerosol mass spectrometry
Characterizing lead-rich particles in Beijing's atmosphere following coal-to-gas conversion: Insights from single particle aerosol mass spectrometry Open
Coal-to-gas (CTG) policies are important energy transformation strategies to address air pollution issues, but how well it improves atmospheric Lead (Pb) pollution remains poorly understood. By the end of 2018, Beijing had achieved coal-fr…
View article: MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony
MISO: Microfluidic protein isolation enables single particle cryo-EM structure determination from a single cell colony Open
Single particle cryo-EM enables reconstructing near-atomic or even atomic resolution 3D maps of proteins by visualizing thousands to a few million purified protein particles embedded in nanometer- thick vitreous ice. This corresponds to pi…
View article: SAM-EM: Real-Time Segmentation for Automated Liquid Phase Transmission Electron Microscopy
SAM-EM: Real-Time Segmentation for Automated Liquid Phase Transmission Electron Microscopy Open
The absence of robust segmentation frameworks for noisy liquid phase transmission electron microscopy (LPTEM) videos prevents reliable extraction of particle trajectories, creating a major barrier to quantitative analysis and to connecting…
View article: Isotopic analysis of Nd nanoparticles using single particle MC-ICP-MS: A comparative study with single particle-ICP-TOF-MS
Isotopic analysis of Nd nanoparticles using single particle MC-ICP-MS: A comparative study with single particle-ICP-TOF-MS Open