Chemical imaging
View article: Chemical Landscape for Tissue Clearing Based on Hydrophilic Reagents
Chemical Landscape for Tissue Clearing Based on Hydrophilic Reagents Open
We describe a strategy for developing hydrophilic chemical cocktails for tissue delipidation, decoloring, refractive index (RI) matching, and decalcification, based on comprehensive chemical profiling. More than 1,600 chemicals were screen…
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Raman Tweezers for Small Microplastics and Nanoplastics Identification in Seawater Open
Our understanding of the fate and distribution of micro- and nano- plastics in the marine environment is limited by the intrinsic difficulties of the techniques currently used for the detection, quantification, and chemical identification …
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Prospects for plasmonic hot spots in single molecule SERS towards the chemical imaging of live cells Open
Single molecule surface enhanced Raman scattering (SM-SERS) is a highly local effect occurring at sharp edges, interparticle junctions and crevices or other geometries with a sharp nanoroughness of plasmonic nanostructures (“hot spots”) fo…
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Nanoscale chemical imaging by photoinduced force microscopy Open
Photoinduced force microscopy resolves nanometer-scale topology with chemical recognition based on material absorption.
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Infrared Imaging and Spectroscopy Beyond the Diffraction Limit Open
Progress in nanotechnology is enabled by and dependent on the availability of measurement methods with spatial resolution commensurate with nanomaterials' length scales. Chemical imaging techniques, such as scattering scanning near-field o…
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Infrared and Raman chemical imaging and spectroscopy at the nanoscale Open
The advent of nanotechnology, and the need to understand the chemical composition at the nanoscale, has stimulated the convergence of IR and Raman spectroscopy with scanning probe methods, resulting in new nanospectroscopy paradigms.
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High Throughput In vivo Analysis of Plant Leaf Chemical Properties Using Hyperspectral Imaging Open
Image-based high-throughput plant phenotyping in greenhouse has the potential to relieve the bottleneck currently presented by phenotypic scoring which limits the throughput of gene discovery and crop improvement efforts. Numerous studies …
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Raman Spectroscopy for Chemical Biology Research Open
In chemical biology research, various fluorescent probes have been developed and used to visualize target proteins or molecules in living cells and tissues, yet there are limitations to this technology, such as the limited number of colors…
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Coherent Raman Scattering Microscopy in Biology and Medicine Open
Advancements in coherent Raman scattering (CRS) microscopy have enabled label-free visualization and analysis of functional, endogenous biomolecules in living systems. When compared with spontaneous Raman microscopy, a key advantage of CRS…
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Perseverance’s Scanning Habitable Environments with Raman and Luminescence for Organics and Chemicals (SHERLOC) Investigation Open
The Scanning Habitable Environments with Raman and Luminescence for Organics and Chemicals (SHERLOC) is a robotic arm-mounted instrument on NASA’s Perseverance rover. SHERLOC has two primary boresights. The Spectroscopy boresight generates…
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Near-Infrared spectroscopy and hyperspectral imaging for non-destructive quality assessment of cereal grains Open
Hyperspectral imaging (HSI) combines spectroscopy and imaging, providing information about the chemical properties of a material and their spatial distribution. It represents an advance of traditional Near-Infrared (NIR) spectroscopy. The …
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Label-free chemical imaging flow cytometry by high-speed multicolor stimulated Raman scattering Open
Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, imaging flow cytometry is a powerful tool in diverse fields including cancer biology, immunology, drug discovery, microbiology, and metabolic en…
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Live-Cell Bioorthogonal Chemical Imaging: Stimulated Raman Scattering Microscopy of Vibrational Probes Open
Innovations in light microscopy have tremendously revolutionized the way researchers study biological systems with subcellular resolution. In particular, fluorescence microscopy with the expanding choices of fluorescent probes has provided…
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Acoustogenic Probes: A New Frontier in Photoacoustic Imaging Open
Photoacoustic imaging (PAI) is a powerful imaging modality capable of mapping the absorption of light in biological tissue via the PA effect. When chromophores are optically excited, subsequent energy loss in the form of heat generates loc…
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Single-Cell Analysis: Visualizing Pharmaceutical and Metabolite Uptake in Cells with Label-Free 3D Mass Spectrometry Imaging Open
Detecting metabolites and parent compound within a cell type is now a priority for pharmaceutical development. In this context, three-dimensional secondary ion mass spectrometry (SIMS) imaging was used to investigate the cellular uptake of…
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Nanoscale simultaneous chemical and mechanical imaging via peak force infrared microscopy Open
A new scanning probe microscopy images the nanoscale world with synergistic force and infrared light at 10-nm spatial resolution.
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Hyperspectral Microscopy of Near-Infrared Fluorescence Enables 17-Chirality Carbon Nanotube Imaging Open
The intrinsic near-infrared photoluminescence (fluorescence) of single-walled carbon nanotubes exhibits unique photostability, narrow bandwidth, penetration through biological media, environmental sensitivity and both chromatic variety and…
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Fundamental developments in infrared spectroscopic imaging for biomedical applications Open
This review covers the rapidly emerging field of infrared chemical imaging focussing on new advances in instrumentation and data acquisition.
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Ultrafast chemical imaging by widefield photothermal sensing of infrared absorption Open
Widefield photothermal microscopy enables ultrafast chemical imaging at submicrometer spatial resolution.
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Volumetric chemical imaging by clearing-enhanced stimulated Raman scattering microscopy Open
Significance Cells form structures and perform functions through intricate 3D tissue organizations. However, due to tissue scattering, coherent Raman microscopy—a powerful method complementary to fluorescence imaging—suffers from limited i…
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Three-dimensional nanoscale molecular imaging by extreme ultraviolet laser ablation mass spectrometry Open
Analytical probes capable of mapping molecular composition at the nanoscale are of critical importance to materials research, biology and medicine. Mass spectral imaging makes it possible to visualize the spatial organization of multiple m…
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Bond-selective imaging by optically sensing the mid-infrared photothermal effect Open
Mid-infrared photothermal microscopy enables vibrational spectroscopic imaging at submicrometer spatial resolution.
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Rapid Identification and Quantification of Microplastics in the Environment by Quantum Cascade Laser-Based Hyperspectral Infrared Chemical Imaging Open
The monitoring of the emerging contaminant, microplastics, in the environment, in water supply, and for food safety is of major interest to science, consumers, and governments. While the chemical analysis of these particles is considered m…
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Nanoscale chemical imaging using tip-enhanced Raman spectroscopy Open
Confocal and surface-enhanced Raman spectroscopy (SERS) are powerful techniques for molecular characterization; however, they suffer from the drawback of diffraction-limited spatial resolution. Tip-enhanced Raman spectroscopy (TERS) overco…
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Hyperspectral imaging spectroscopy: a promising method for the biogeochemical analysis of lake sediments Open
We investigate the potential of hyperspectral imaging spectrometry for the analysis of fresh sediment cores. A sediment-core-scanning system equipped with a camera working in the visual to near-infrared range (400 to 1000 nm) is described …
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Nondestructive, high-resolution, chemically specific 3D nanostructure characterization using phase-sensitive EUV imaging reflectometry Open
A new imaging technique enables nondestructive, 3D characterization of nanostructures, their composition, and interfaces.
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Imaging the Unimaginable: Desorption Electrospray Ionization – Imaging Mass Spectrometry (DESI-IMS) in Natural Product Research Open
Imaging mass spectrometry (IMS) has recently established itself in the field of “spatial metabolomics.” Merging the sensitivity and fast screening of high-throughput mass spectrometry with spatial and temporal chemical information, IMS vis…
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The effect of weathering environments on microplastic chemical identification with Raman and IR spectroscopy: Part I. polyethylene and polypropylene Open
Chemical identification of microplastics is a crucial step in understanding the sources of microplastics and studying the effects of microplastic pollution. A major challenge to microplastics identification is that weathered microplastics …
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Synchrotron macro ATR-FTIR microspectroscopy for high-resolution chemical mapping of single cells Open
Coupling synchrotron IR beam to an ATR element enhances spatial resolution suited for high-resolution single cell analysis in biology, medicine and environmental science.
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Video-rate, mid-infrared hyperspectral upconversion imaging Open
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