Blastema
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Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration Open
How the axolotl makes a new limb Unlike most vertebrate limbs, the axolotl limb regenerates the skeleton after amputation. Dermal and interstitial fibroblasts have been thought to provide sources for skeletal regeneration, but it has been …
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The art of fin regeneration in zebrafish Open
The zebrafish fin provides a valuable model to study the epimorphic type of regeneration, whereby the amputated part of the appendage is nearly perfectly replaced. To accomplish fin regeneration, two reciprocally interacting domains need t…
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Macrophages and fibroblasts during inflammation and tissue repair in models of organ regeneration Open
This review provides a concise summary of the changing phenotypes of macrophages and fibroblastic cells during the local inflammatory response, the onset of tissue repair, and the resolution of inflammation which follow injury to an organ.…
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A Magnesium‐Enriched 3D Culture System that Mimics the Bone Development Microenvironment for Vascularized Bone Regeneration Open
The redevelopment/regeneration pattern of amputated limbs from a blastema in salamander suggests that enhanced regeneration might be achieved by mimicking the developmental microenvironment. Inspired by the discovery that the expression of…
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The axolotl limb blastema: cellular and molecular mechanisms driving blastema formation and limb regeneration in tetrapods Open
The axolotl is one of the few tetrapods that are capable of regenerating complicated biological structures, such as complete limbs, throughout adulthood. Upon injury the axolotl generates a population of regeneration-competent limb progeni…
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Signalling by Transforming Growth Factor Beta Isoforms in Wound Healing and Tissue Regeneration Open
Transforming growth factor beta (TGFβ) signalling is essential for wound healing, including both non-specific scar formation and tissue-specific regeneration. Specific TGFβ isoforms and downstream mediators of canonical and non-canonical s…
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Transcriptomic landscape of the blastema niche in regenerating adult axolotl limbs at single-cell resolution Open
Regeneration of complex multi-tissue structures, such as limbs, requires the coordinated effort of multiple cell types. In axolotl limb regeneration, the wound epidermis and blastema have been extensively studied via histology, grafting, a…
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Localized epigenetic silencing of a damage-activated WNT enhancer limits regeneration in mature Drosophila imaginal discs Open
Many organisms lose the capacity to regenerate damaged tissues as they mature. Damaged Drosophila imaginal discs regenerate efficiently early in the third larval instar (L3) but progressively lose this ability. This correlates with reduced…
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During <i>Drosophila</i> disc regeneration, JAK/STAT coordinates cell proliferation with Dilp8-mediated developmental delay Open
Significance The larval imaginal discs of the fruit fly are capable of fully regenerating mechanically damaged parts. Wound healing is initiated by the JNK signaling pathway. We followed the subsequent formation of the regenerating blastem…
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Mechanisms of urodele limb regeneration Open
This review explores the historical and current state of our knowledge about urodele limb regeneration. Topics discussed are (1) blastema formation by the proteolytic histolysis of limb tissues to release resident stem cells and mononuclea…
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Lgr6 marks nail stem cells and is required for digit tip regeneration Open
Significance Although full mammalian limbs do not regenerate after amputation, the fingertips of select mammalian species do. Understanding digit tip regeneration at the molecular level can potentially provide insight into designing transl…
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Cross-species metabolomic analysis identifies uridine as a potent regeneration promoting factor Open
Regenerative capacity declines throughout evolution and with age. In this study, we asked whether metabolic programs underlying regenerative capability might be conserved across species, and if so, whether such metabolic drivers might be h…
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Distinct mechanisms underlie oral vs aboral regeneration in the cnidarian Hydractinia echinata Open
Cnidarians possess remarkable powers of regeneration, but the cellular and molecular mechanisms underlying this capability are unclear. Studying the hydrozoan Hydractinia echinata we show that a burst of stem cell proliferation occurs foll…
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Characterization of the Zebrafish Cell Landscape at Single-Cell Resolution Open
Zebrafish have been found to be a premier model organism in biological and regeneration research. However, the comprehensive cell compositions and molecular dynamics during tissue regeneration in zebrafish remain poorly understood. Here, w…
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Macrophages are required to coordinate mouse digit tip regeneration Open
In mammals, macrophages are known to play a major role in tissue regeneration. These cells contribute to inflammation, histolysis, re-epithelialization, re-vascularization and cell proliferation. While macrophages have been shown to be ess…
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The Drosophila Duox maturation factor is a key component of a positive feedback loop that sustains regeneration signaling Open
Regenerating tissue must initiate the signaling that drives regenerative growth, and sustain that signaling long enough for regeneration to complete. How these key signals are sustained is unclear. To gain a comprehensive view of the chang…
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Novel monoclonal antibodies to study tissue regeneration in planarians Open
The monoclonal antibodies described in this paper will be a valuable resource for planarian research. These antibodies have the potential to be used to better understand planarian biology and to characterize phenotypes following RNAi exper…
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Ear wound regeneration in the African spiny mouse <i>Acomys cahirinus</i> Open
While regeneration occurs in a number of taxonomic groups across the Metazoa, there are very few reports of regeneration in mammals, which generally respond to wounding with fibrotic scarring rather than regeneration. A recent report descr…
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Regenerating zebrafish fin epigenome is characterized by stable lineage-specific DNA methylation and dynamic chromatin accessibility Open
Background Zebrafish can faithfully regenerate injured fins through the formation of a blastema, a mass of proliferative cells that can grow and develop into the lost body part. After amputation, various cell types contribute to blastema f…
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Lizard tail skeletal regeneration combines aspects of fracture healing and blastema-based regeneration Open
Lizards are amniotes with the remarkable ability to regenerate amputated tails. The early regenerated lizard tail forms a blastema, and the regenerated skeleton consists of a cartilage tube (CT) surrounding the regenerated spinal cord. The…
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The mammalian blastema: regeneration at our fingertips Open
In the mouse, digit tip regeneration progresses through a series of discrete stages that include inflammation, histolysis, epidermal closure, blastema formation, and redifferentiation. Recent studies reveal how each regenerative stage infl…
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Live imaging reveals the progenitors and cell dynamics of limb regeneration Open
Regeneration is a complex and dynamic process, mobilizing diverse cell types and remodelling tissues over long time periods. Tracking cell fate and behaviour during regeneration in active adult animals is especially challenging. Here, we e…
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Deep evolutionary origin of limb and fin regeneration Open
Salamanders and lungfishes are the only sarcopterygians (lobe-finned vertebrates) capable of paired appendage regeneration, regardless of the amputation level. Among actinopterygians (ray-finned fishes), regeneration after amputation at th…
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Effects of electrical stimulation on rat limb regeneration, a new look at an old model Open
Limb loss is a devastating disability and while current treatments provide aesthetic and functional restoration, they are associated with complications and risks. The optimal solution would be to harness the body’s regenerative capabilitie…
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Oscarella lobularis (Homoscleromorpha, Porifera) Regeneration: Epithelial Morphogenesis and Metaplasia Open
Sponges are known to possess remarkable reconstitutive and regenerative abilities ranging from common wounding or body part regeneration to more impressive re-building of a functional body from dissociated cells. Among the four sponge clas…
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Transdifferentiation is a driving force of regeneration in<i>Halisarca dujardini</i>(Demospongiae, Porifera) Open
The ability to regenerate is widespread in the animal kingdom, but the regenerative capacities and mechanisms vary widely. To understand the evolutionary history of the diverse regeneration mechanisms, the regeneration processes must be st…
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Yap control of tissue growth relies on cell density and F-actin in zebrafish fin regeneration Open
Caudal fin regeneration is characterized by a proliferation boost in the mesenchymal blastema controlled precisely in time and space. This allows a gradual and robust restoration of original fin size. However, how this is established and r…
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Cooperative inputs of Bmp and Fgf signaling induce tail regeneration in urodele amphibians Open
Urodele amphibians have remarkable organ regeneration ability. They can regenerate not only limbs but also a tail throughout their life. It has been demonstrated that the regeneration of some organs are governed by the presence of neural t…
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A Conserved MicroRNA Regulatory Circuit Is Differentially Controlled during Limb/Appendage Regeneration Open
Our extensive analysis of RNA-seq transcriptome profiling studies in three regenerative animal models, that diverged in evolution ~420 million years ago, reveals a common miRNA-regulated genetic network of blastema genes. These comparative…
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Identification of regenerative roadblocks via repeat deployment of limb regeneration in axolotls Open
Axolotl salamanders are powerful models for understanding how regeneration of complex body parts can be achieved, whereas mammals are severely limited in this ability. Factors that promote normal axolotl regeneration can be examined in mam…