Aptamer ≈ Aptamer
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PLIP 2021: expanding the scope of the protein–ligand interaction profiler to DNA and RNA Open
With the growth of protein structure data, the analysis of molecular interactions between ligands and their target molecules is gaining importance. PLIP, the protein–ligand interaction profiler, detects and visualises these interactions an…
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Aptamer–field-effect transistors overcome Debye length limitations for small-molecule sensing Open
Transistor sensing in salt solutions Molecular binding to receptors on the surface of field-effect transistors (FETs) can be sensed through changes in transconductance. However, the saline solutions typically used with biomolecules create …
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Active Targeting Strategies Using Biological Ligands for Nanoparticle Drug Delivery Systems Open
Targeting nanoparticle (NP) carriers to sites of disease is critical for their successful use as drug delivery systems. Along with optimization of physicochemical properties, researchers have focused on surface modification of NPs with bio…
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Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets Open
Beyond its extraordinary genome editing ability, the CRISPR-Cas systems have opened a new era of biosensing applications due to its high base resolution and isothermal signal amplification. However, the reported CRISPR-Cas sensors are larg…
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Synthetic, Switchable Enzymes Open
The construction of switchable, radiation-controlled, aptameric enzymes - “swenzymes” - is, in principle, feasible. We propose a strategy to make such catalysts from 2 (or more) aptamers each selected to bind specifically to one of the sub…
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Recent Advances in Biosensor Technology for Potential Applications – An Overview Open
Imperative utilization of biosensors has acquired paramount importance in the field of drug discovery, biomedicine, food safety standards, defense, security, and environmental monitoring. This has led to the invention of precise and powerf…
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Recent Advances in Aptamer Discovery and Applications Open
Aptamers are short, single-stranded DNA, RNA, or synthetic XNA molecules that can be developed with high affinity and specificity to interact with any desired targets. They have been widely used in facilitating discoveries in basic researc…
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Aptamers as Therapeutics Open
Aptamers are single-stranded nucleic acid molecules that bind to and inhibit proteins and are commonly produced by systematic evolution of ligands by exponential enrichment (SELEX). Aptamers undergo extensive pharmacological revision, whic…
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The Limitless Future of RNA Therapeutics Open
Recent advances in the generation, purification and cellular delivery of RNA have enabled development of RNA-based therapeutics for a broad array of applications. RNA therapeutics comprise a rapidly expanding category of drugs that will ch…
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Three decades of nucleic acid aptamer technologies: Lessons learned, progress and opportunities on aptamer development Open
Aptamers are short single-stranded nucleic acid sequences capable of binding to target molecules in a way similar to antibodies. Due to various advantages such as prolonged shelf life, low batch to batch variation, low/no immunogenicity, f…
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Recent Advances in SELEX Technology and Aptamer Applications in Biomedicine Open
Aptamers are short DNA/RNA oligonucleotides capable of binding to target molecules with high affinity and specificity. The process of selecting an aptamer is called Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Thanks …
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Analyte-Responsive Hydrogels: Intelligent Materials for Biosensing and Drug Delivery Open
Nature has mastered the art of molecular recognition. For example, using synergistic non-covalent interactions, proteins can distinguish between molecules and bind a partner with incredible affinity and specificity. Scientists have develop…
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Aptasensor with Expanded Nucleotide Using DNA Nanotetrahedra for Electrochemical Detection of Cancerous Exosomes Open
Exosomes are extracellular vesicles (50-100 nm) circulating in biofluids as intercellular signal transmitters. Although the potential of cancerous exosomes as tumor biomarkers is promising, sensitive and rapid detection of exosomes remains…
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Discovery of Aptamers Targeting the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein Open
The World Health Organization has declared the outbreak of a novel coronavirus (SARS-CoV-2 or 2019-nCoV) as a global pandemic. However, the mechanisms behind the coronavirus infection are not yet fully understood, nor are there any targete…
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Advances and Challenges in Small‐Molecule DNA Aptamer Isolation, Characterization, and Sensor Development Open
Aptamers are short oligonucleotides isolated in vitro from randomized libraries that can bind to specific molecules with high affinity, and offer a number of advantages relative to antibodies as biorecognition elements in biosensors. Howev…
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Getting into the brain: liposome-based strategies for effective drug delivery across the blood–brain barrier Open
This review summarizes articles that have been reported in literature on liposome-based strategies for effective drug delivery across the blood-brain barrier. Due to their unique physicochemical characteristics, liposomes have been widely …
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Orientation and characterization of immobilized antibodies for improved immunoassays (Review) Open
Orientation of surface immobilized capture proteins, such as antibodies, plays a critical role in the performance of immunoassays. The sensitivity of immunodiagnostic procedures is dependent on presentation of the antibody, with optimum pe…
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Small-Molecule Binding Aptamers: Selection Strategies, Characterization, and Applications Open
Aptamers are single-stranded, synthetic oligonucleotides that fold into 3-dimensional shapes capable of binding non-covalently with high affinity and specificity to a target molecule. They are generated via an in vitro process known as the…
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A Global Review on Short Peptides: Frontiers and Perspectives Open
Peptides are fragments of proteins that carry out biological functions. They act as signaling entities via all domains of life and interfere with protein-protein interactions, which are indispensable in bio-processes. Short peptides includ…
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Aptamers Chemistry: Chemical Modifications and Conjugation Strategies Open
Soon after they were first described in 1990, aptamers were largely recognized as a new class of biological ligands that can rival antibodies in various analytical, diagnostic, and therapeutic applications. Aptamers are short single-strand…
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Noble metal nanoparticles in biosensors: recent studies and applications Open
The aim of this review is to cover advances in noble metal nanoparticle (MNP)-based biosensors and to outline the principles and main functions of MNPs in different classes of biosensors according to the transduction methods employed. The …
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Aptamers in the Therapeutics and Diagnostics Pipelines Open
Aptamers are short single-stranded DNA or RNA oligonucleotides that can selectively bind to small molecular ligands or protein targets with high affinity and specificity, by acquiring unique three-dimensional structures. Aptamers have the …
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Upconversion Luminescence-Activated DNA Nanodevice for ATP Sensing in Living Cells Open
Designer DNA nanodevices have attracted extensive interest for detection of specific targets in living cells. However, it still remains a great challenge to construct DNA sensing devices that can be activated at desired time with a remotel…
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Wearable aptamer-field-effect transistor sensing system for noninvasive cortisol monitoring Open
A smartwatch was developed for noninvasive stress biomarker data acquisition that provides real-time feedback to the wearer.
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Chemistry Can Make Strict and Fuzzy Controls for Bio-Systems: DNA Nanoarchitectonics and Cell-Macromolecular Nanoarchitectonics Open
In this review, we introduce two kinds of bio-related nanoarchitectonics, DNA nanoarchitectonics and cell-macromolecular nanoarchitectonics, both of which are basically controlled by chemical strategies. The former DNA-based approach would…
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The biological applications of DNA nanomaterials: current challenges and future directions Open
DNA, a genetic material, has been employed in different scientific directions for various biological applications as driven by DNA nanotechnology in the past decades, including tissue regeneration, disease prevention, inflammation inhibiti…
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Chemical Modifications of Nucleic Acid Aptamers for Therapeutic Purposes Open
Nucleic acid aptamers have minimal immunogenicity, high chemical synthesis production, low cost and high chemical stability when compared with antibodies. However, the susceptibility to nuclease degradation, rapid excretion through renal f…
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Biosensors and sensors for dopamine detection Open
Dopamine is a key catecholamine neurotransmitter and it has critical roles in the function of the human central nervous system. Abnormal release of dopamine is related to neurological diseases and depression. Therefore, it is necessary to …
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Detection and beyond: challenges and advances in aptamer-based biosensors Open
This review provides insight into how current problems of biosensors can be solved by the use of nucleic acid aptamers.
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SELEX: Critical factors and optimization strategies for successful aptamer selection Open
Within the last decade, the application range of aptamers in biochemistry and medicine has expanded rapidly. More than just a replacement for antibodies, these intrinsically structured RNA‐ or DNA‐oligonucleotides show great potential for …